Journal of Marine Bioscience and Biotechnology (한국해양바이오학회지)

The Korean Society for Marine Biotechnology (한국해양바이오학회)
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Whitening effect of red stage Haematococcus pluvialis ethanol extract

Red stage Haematococcus pluvialis 에탄올 추출물의 미백효과

  • Seo-Rin Jung (Department of Medical Science, Soonchunhyang University) ;
  • Wook-Chul Kim (Department of Medical Science, Soonchunhyang University) ;
  • Yun-Su Lee (Department of Medical Science, Soonchunhyang University) ;
  • Taesoo Kim (Department of Biology, Soonchunhyang University) ;
  • Sang Mok Jung (Research Institute for Basic Science, Soonchunhyang University) ;
  • Hyun Woung Shin (Department of Biology, Soonchunhyang University) ;
  • Seung-Hong Lee (Department of Medical Science, Soonchunhyang University)
  • 정서린 (순천향대학교 의료과학과) ;
  • 김욱철 (순천향대학교 의료과학과) ;
  • 이윤수 (순천향대학교 의료과학과) ;
  • 김태수 (순천향대학교 생명과학과) ;
  • 정상목 (순천향대학교 기초과학연구소) ;
  • 신현웅 (순천향대학교 생명과학과) ;
  • 이승홍 (순천향대학교 의료과학과)
  • Received : 2025.03.20
  • Accepted : 2025.05.19
  • Published : 2025.06.30
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Abstract

Haematococcus pluvialis reportedly exhibits various beneficial effects, such as anti-oxidant and anti-obesity properties. However, its anti-melanogenic effect, particularly that of red-stage H. pluvialis ethanol extract (HPEE), has not yet been investigated in B16F10 melanoma cells or in a zebrafish model. Therefore, this study aimed to investigate the anti-melanogenic activity of HPEE. The results showed that HPEE strongly inhibited Tyrosinase (TYR) activity. In addition, HPEE treatment significantly reduced cellular tyrosinase activity and melanin content in a dose-dependent manner in α-melanocyte-stimulating hormone (α-MSH)-stimulated B16F10 melanoma cells without inducing cytotoxicity. HPEE also downregulated the expression of melanogenesis-related proteins, including TYR, tyrosinase-related protein-1 (TRP-1), and tyrosinase-related protein-2 (TRP-2), by suppressing the microphthalmia-associated transcription factor in α-MSH-stimulated B16F10 melanoma cells. In an in vivo zebrafish model, HPEE significantly inhibited melanin synthesis in a dose-dependent manner without inducing developmental toxicity. These findings indicate that HPEE exhibits anti-melanogenic activity in vitro and in vivo, and may serve as a potential skin-whitening agent in pharmaceutical and cosmeceutical applications.

Keywords

Acknowledgement

본 연구는 연구개발특구진흥재단의 '지역혁신 메가프로젝트' 사업으로 수행되었습니다.(과제명:해양바이오 전략소재 메타 플랫폼화 원천기술개발, 과제번호: 2023-DD-UP-0007)

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