Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Lactobacillus rhamnosus GR-1 alleviates Escherichia coli-triggered bovine endometrial epithelial cells damage via the reactive oxygen species-mitochondrial pathway

  • Xiaowei Feng (College of Veterinary Medicine, Hebei Agricultural University) ;
  • Yan Li (College of Veterinary Medicine, Hebei Agricultural University) ;
  • Xiangfu Wen (College of Veterinary Medicine, Hebei Agricultural University) ;
  • Mingque Feng (College of Veterinary Medicine, Hebei Agricultural University) ;
  • Tianxiong Jin (College of Veterinary Medicine, Hebei Agricultural University) ;
  • Muhammad Shahid (Center of Microbiology and Biotechnology, Veterinary Research Institute) ;
  • Yan Sun (College of Veterinary Medicine, Hebei Agricultural University) ;
  • Jiawei Liu (College of Veterinary Medicine, Hebei Agricultural University) ;
  • Bei Liu (College of Veterinary Medicine, Hebei Agricultural University) ;
  • Jia Cheng (College of Veterinary Medicine, Hebei Agricultural University) ;
  • Mingchao Liu (College of Veterinary Medicine, Hebei Agricultural University)
  • Received : 2025.01.15
  • Accepted : 2025.05.15
  • Published : 2025.09.01
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Abstract

Objective: The objective of this study was to evaluate the function of the reactive oxygen species (ROS)-mitochondrial pathway in attenuating Escherichia coli (E. coli) induced apoptosis in bovine endometrial epithelial cells (BENDs) by Lactobacillus rhamnosus (L. rhamnosus) GR-1. Methods: The BENDs were exposed to preincubation with and without L. rhamnosus GR-1 for 3 hours (h) and they were later subject to E. coli for 6 h. The release of lactate dehydrogenase (LDH), the expression of oxidative factors, adhesion and invasion of E. coli, the expression of mitochondrial membrane potential (MMP), apoptotic rate and apoptosis-associated protein expression were observed. Then, ROS expression, MMP level and cell rate apoptosis rate were further detected after the intervention of antioxidant n-acetyl-l-cysteine (NAC). Results: L. rhamnosus GR-1 was capable of obviously alleviating the content of LDH, ROS expression, E. coli adhesion and invasion, the apoptotic rate and MDA concentrations in BENDs induced by E. coli (p<0.01). In addition, L. rhamnosus GR-1 could notably promote the levels of antioxidant factors (SOD, GSH, T-AOC) (p<0.01), inhibit the depolarization of MMP (p<0.01), and levels of apoptosis-related factors (Bax, Caspase-3 and Cyt-c), and promote anti-apoptosis factor (Bcl-2) (p<0.01). Moreover, after the intervention of antioxidant NAC, ROS expression, MMP depolarization, and apoptotic rate induced by E. coli in BENDs were decreased (p<0.01). Conclusion: In E. coli-induced apoptosis in BENDs, therapeutic strategies aimed at down-regulating ROS and MDA and up-regulating antioxidant factors may maintain mitochondrial function and provide theoretical support for the use of probiotics in bovine endometritis.

Keywords

Acknowledgement

The authors want to deliver our thanks to laboratory members who assisted us to enhance the paper with their professional technical assistance, helpful comments and advice.

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