Applied Chemistry for Engineering (공업화학)

The Korean Society of Industrial and Engineering Chemistry (한국공업화학회)
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Thunberginol C Suppresses MMP-1 Expression in Hs68 Human Dermal Fibroblasts

  • Si Hyeon Kim (Department of Cosmetic Science, Semyung University) ;
  • Yong Min Kim (Department of Cosmetic Science, Semyung University)
  • Received : 2025.08.29
  • Accepted : 2025.09.30
  • Published : 2025.10.10
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Abstract

Aging, characterized by deep wrinkles, is caused by matrix metalloproteinases (MMPs) that are induced by reactive oxygen species (ROS) and cytokines. In particular, the expression of MMP-1 decomposes collagen and accelerates aging. In this study, we evaluated the inhibitory effect of Thunberginol C (TBC) on tumor necrosis factor-𝛼 (TNF-𝛼)-induced MMP-1 expression in human dermal fibroblasts (Hs68 cells). Quantitative real-time PCR and ELISA demonstrated that TBC (10~20 µM) significantly suppresses MMP-1 expression. In contrast, TBC did not affect the phosphorylation of ERK or p38 in the MAPK pathway. However, it effectively inhibited JNK phosphorylation and consequently reduced the activation of the AP-1 components c-Jun and c-Fos. DCF-DA assays confirmed that TBC confers its antioxidant effect by reducing intracellular ROS production. These results suggest that TBC can be used to delay skin aging.

Keywords

Acknowledgement

This research was supported by the Regional Innovation System & Education (RISE) program through the (Chungbuk Regional Innovation System & Education Center), funded by the Ministry of Education (MOE) and the (Chungcheongbuk-do), Republic of Korea.(2025-RISE-11-008-03)

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