Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
권호 표지
DOI QR코드

DOI QR Code

Field Efficacy of Domestically Cultivated Dalmatian Pyrethrum (Tanacetum cinerariifolium) Extract against Myzus persicae and Palpita indica in Pepper and Cucumber Fields

국내 재배 달마시안 제충국(Tanacetum cinerariifolium) 추출물의 포장 시험을 통한 복숭아혹진딧물 및 목화바둑명나방에 대한 방제 효과

  • Ka Hee Cho (Digital Crop Hospital Research Center, Chonnam National University) ;
  • Jin Hee Lee (Environment-Friendly Agriculture Research Institute, Jeollanamdo Agricultural Research & Extension Services) ;
  • Young Cheol Kim (Digital Crop Hospital Research Center, Chonnam National University)
  • 조가희 (전남대학교 디지털작물병원연구센터) ;
  • 이진희 (전라남도농업기술원 친환경농업연구소) ;
  • 김영철 (전남대학교 디지털작물병원연구센터)
  • Received : 2026.05.11
  • Accepted : 2026.05.26
  • Published : 2026.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

Botanical insecticides, particularly extracts from Dalmatian pyrethrum, are considered eco-friendly alternatives to synthetic pesticides. This study evaluated the field efficacy of a 95% ethanol extract of domestically cultivated pyrethrum flowers in 2025. Field trials were conducted in pepper and cucumber fields to assess control efficacy against Myzus persicae in pepper, and the lepidopteran pest Palpita indica in cucumber. In pepper fields, pyrethrum extract exhibited relatively high efficacy against M. persicae, achieving a control value of 79.5 ± 4.5%, second only to the chemical pesticide (cyantraniliprole) with 93.1 ± 4.0%. Against P. indica in cucumber fields, pyrethrum extract showed low efficacy with a control value of 12.8 ± 23.8%, whereas the chemical treatment achieved 73.4 ± 27.1%. These results indicate that domestically cultivated pyrethrum extract is effective against M. persicae under pepper cultivation, but shows limited efficacy against lepidopteran pests such as P. indica. Therefore, its practical application may be suitable for the green peach aphid, while additional strategies such as improved formulation or combined application with microbial insecticides are required to improve efficacy against chewing insects.

식물성 살충제는 화학 살충제의 잔류, 비표적 생물에 대한 영향, 약제저항성 발달 등의 문제를 보완할 수 있는 친환경 방제 수단으로 주목받고 있다. 본 연구에서는 국내에서 재배된 달마시안 제충국(Tanacetum cinerariifolium) 꽃에서 95% 에탄올로 추출한 피레트린 함유 추출물의 포장 방제 효과를 평가하였다. 2025년 전라남도 나주시 시험포장에서 고추와 오이를 대상으로 무처리, 화학 살충제(cyantraniliprole), 유기농자재 마늘 추출물, 제충국 추출물의 4처리를 완전임의배치법으로 배치하여 수행하였다. 고추 포장에서 발생한 복숭아혹진딧물(Myzus persicae)에 대해 제충국 추출물이 79.5 ± 4.5%의 방제가를 나타내어 화학 살충제(93.1±4.0%) 다음으로 높은 방제 효과를 보였다. 그러나 오이 포장의 목화바둑명나방(Palpita indica) 유충에 대해서는 제충국 추출물의 방제가가 12.8±23.8%로 화학 살충제(73.4 ± 27.1%)에 비해 매우 낮은 효과를 보였다. 이러한 결과는 국내 재배 제충국 추출물이 고추에 발생하는 복숭아혹진딧물에 대해서는 실용적 방제 잠재력을 가지나, 저작성 나비목 해충에 대해서는 단독 처리만으로는 한계가 있음을 시사한다. 향후 제형 개선 및 미생물 살충제와의 병용 처리 등 통합적 방제 전략이 필요할 것으로 판단된다.

Keywords

Acknowledgement

본 연구는 농촌진흥청 농업과학기술 연구개발사업(과제번호: RS-2022-RD010417)의 지원을 받아 수행되었습니다.

References

  1. Adamczyk-Szabela, D., Anielak, P., Wolf, W.M., 2013. Influence of digestion procedure and residual carbon on manganese, copper, and zinc determination in herbal matrices by atomic absorption spectrometry. J. Anal. Methods Chem. 2013, 980101.
  2. Antonious, G.F., 2004. Persistence of pyrethrum on leaves and soil exposed to sunlight. J. Environ. Sci. Health B 39, 211-225.
  3. Arogundade, O., Ajose, T., Matthew, J.O., Osijo, I.A., 2021. Current and emerging pests and diseases of cucumber (Cucumis sativus L.) in Africa, in: Wai-Kuan Ng, H. (Ed.), Cucumber economic values and its cultivation and breeding. IntechOpen, London, pp. 179-198.
  4. Bahlai, C.A., Xue, Y., McCreary, C.M., Schaafsma, A.W., Hallett, R.H., 2010. Choosing organic pesticides over synthetic pesticides may not effectively mitigate environmental risk in soybeans. PLoS ONE 5, e11250. https://doi.org/10.1371/journal.pone.0011250
  5. Bass, C., Nauen, R., 2023. The molecular mechanisms of insecticide resistance in aphid crop pests. Insect Biochem. Mol. Biol. 156, 103937. https://doi.org/10.1016/j.ibmb.2023.103937
  6. Bass, C., Puinean, A.M., Zimmer, C.T., Denholm, I., Field, L.M., Foster, S.P., Gutbrod, O., Nauen, R., Slater, R., Williamson, M.S., 2014. The evolution of insecticide resistance in the peach potato aphid, Myzus persicae. Insect Biochem. Mol. Biol. 51, 41-51. https://doi.org/10.1016/j.ibmb.2014.05.003
  7. Blackman, R.L., Eastop, V.F., 2007. Taxonomic issues, in: van Emden, H.F., Harrington, R. (Eds.), Aphids as crop pests. CABI, Wallingford, pp. 1-29.
  8. Capinera, J.L., 2001. Handbook of vegetable pests. Academic Press, San Diego.
  9. Casida, J.E., 1980. Pyrethrum flowers and pyrethroid insecticides. Environ. Health Perspect. 34, 189-202. https://doi.org/10.1289/ehp.8034189
  10. Casida, J.E., Quistad, G.B., 1995. Pyrethrum flowers: Production, chemistry, toxicology, and uses. Oxford University Press, New York.
  11. Cho, K.H., Kim, H.J., Lee, J.H., Kim, Y.C., 2024. Evaluation of insecticidal ingredients and efficacy of extracts from pyrethrum flowers cultivated in South Korea. Korean J. Appl. Entomol. 63, 253-263.
  12. Cordova, D., Benner, E.A., Sacher, M.D., Rauh, J.J., Sopa, J.S., Lahm, G.P., Selby, T.P., Stevenson, T.M., Flexner, L., Gutteridge, S., Rhoades, D.F., Wu, L., Smith, R.M., Tao, Y., 2006. Anthranilic diamides: A new class of insecticides with a novel mode of action, ryanodine receptor activation. Pestic. Biochem. Physiol. 84, 196-214. https://doi.org/10.1016/j.pestbp.2005.07.005
  13. Després, L., David, J.P., Gallet, C., 2007. The evolutionary ecology of insect resistance to plant chemicals. Trends Ecol. Evol. 22, 298-307. https://doi.org/10.1016/j.tree.2007.02.010
  14. Elliott, M., 1976. Properties and applications of pyrethroids. Environ. Health Perspect. 14, 1-13. https://doi.org/10.1289/ehp.76141
  15. Field, L.M., Emyr Davies, T.G., O'Reilly, A.O., Williamson, M.S., Wallace, B.A., 2017. Voltage-gated sodium channels as targets for pyrethroid insecticides. Eur. Biophys. J. 46, 675-679. https://doi.org/10.1007/s00249-016-1195-1
  16. Fu, Y., Colella, S., Uzest, M., 2025. Hemipteran vectors of styletborne plant viruses: aphids lead the charge. Insect Sci. 32, 1-18. https://doi.org/10.1111/1744-7917.70187
  17. Giné, A., López-Gómez, M., Vela, M.D., Ornat, C., Talavera, M., Verdejo-Lucas, S., Sorribas, F.J., 2014. Thermal requirements and population dynamics of root-knot nematodes on cucumber and yield losses under protected cultivation. Plant Pathol. 63, 1446-1453. https://doi.org/10.1111/ppa.2014.63.issue-6
  18. Isman, M.B., 2006. Botanical insecticides, deterrents, and repellents in modern agriculture and an increasingly regulated world. Annu. Rev. Entomol. 51, 45-66. https://doi.org/10.1146/ento.2006.51.issue-1
  19. Kang, D.H., Lee, Y., Moon, H.H., Kim, S.E., Koo, H.N., Kim, H.K., Kim, G.H., 2024. Combined effect of afidopyropen, chlorfenapyr and cyantraniliprole to insecticide-resistant cotton aphid, Aphis gossypii (Hemiptera: Aphididae). Korean J. Appl. Entomol. 63, 53-61.
  20. Konecka, E., Kaznowski, A., Grzesiek, W., Nowicki, P., Czarniewska, E., Baranek, J., 2020. Synergistic interaction between carvacrol and Bacillus thuringiensis crystalline proteins against Cydia pomonella and Spodoptera exigua. BioControl 65, 447-460. https://doi.org/10.1007/s10526-020-10011-4
  21. Kostić, M., Popović, Z., Brkić, D., Milanović, S., Sivčev, I., Stanković, S., 2008. Larvicidal and antifeedant activity of some plantderived compounds to Lymantria dispar L. (Lepidoptera: Lymantriidae). Bioresour. Technol. 99, 7897-7901. https://doi.org/10.1016/j.biortech.2008.02.010
  22. Li, S., Xu, C., Du, G., Wang, G., Tu, X., Zhang, Z., 2021. Synergy in efficacy of Artemisia sieversiana crude extract and Metarhizium anisopliae on resistant Oedaleus asiaticus. Front. Physiol. 12, 642893. https://doi.org/10.3389/fphys.2021.642893
  23. Lybrand, D.B., Xu, H., Last, R.L., Pichersky, E., 2020. How plants synthesize pyrethrins: safe and biodegradable insecticides. Trends Plant Sci. 25, 1240-1251. https://doi.org/10.1016/j.tplants.2020.06.012
  24. Mantzoukas, S., Eliopoulos, P.A., 2020. Endophytic entomopathogenic fungi: A valuable biological control tool against plant pests. Appl. Sci. 10, 360. https://doi.org/10.3390/app10010360
  25. Matsuo, N., 2019. Discovery and development of pyrethroid insecticides. Proc. Jpn. Acad. Ser. B Phys. Biol. Sci. 95, 378-400. https://doi.org/10.2183/pjab.95.027
  26. Moon, H.H., Lee, Y., Kang, D.H., Kim, S.E., Kim, H.K., Koo, H.N., Kim, G.H., 2024. Resistance monitoring and analysis of point mutations to λ-cyhalothrin, imidacloprid, and flupyradifurone in field-collected populations of Myzus persicae (Hemiptera: Aphididae). Korean J. Appl. Entomol. 63, 33-42.
  27. Ngegba, P.M., Cui, G., Khalid, M.Z., Zhong, G., 2022. Use of botanical pesticides in agriculture as an alternative to synthetic pesticides. Agriculture 12, 600. https://doi.org/10.3390/agriculture12050600
  28. Ochieng, T.A., Akutse, K.S., Ajene, I.J., Kilalo, D.C., Muiru, M., Khamis, F.M., 2024. Interactions between Bacillus thuringiensis and selected plant extracts for sustainable management of Phthorimaea absoluta. Sci. Rep. 14, 9299. https://doi.org/10.1038/s41598-024-60140-4
  29. Park, C.G., Kim, H.Y., Lee, J.H., 2009. Sex pheromone composition of the cotton caterpillar, Palpita indica (Lepidoptera: Pyralidae), in Korea. J. Asia-Pac. Entomol. 12, 53-57. https://doi.org/10.1016/j.aspen.2009.07.003
  30. Pavela, R., 2009. Effectiveness of some botanical insecticides against Spodoptera littoralis Boisduvala (Lepidoptera: Noctuidae), Myzus persicae Sulzer (Hemiptera: Aphididae) and Tetranychus urticae Koch (Acari: Tetranychidae). Plant Prot. Sci. 45, 161-167. https://doi.org/10.17221/16/2009-PPS
  31. Pavela, R., 2016. History, presence and perspective of using plant extracts as commercial botanical insecticides and farm products for protection against insects - a review. Plant Prot. Sci. 52, 229-241. https://doi.org/10.17221/31/2016-PPS
  32. Pavela, R., Benelli, G., 2016. Essential oils as ecofriendly biopesticides? Challenges and constraints. Trends Plant Sci. 21, 1000-1007. https://doi.org/10.1016/j.tplants.2016.10.005
  33. Pigott, C.R., Ellar, D.J., 2007. Role of receptors in Bacillus thuringiensis crystal toxin activity. Microbiol. Mol. Biol. Rev. 71, 255-281. https://doi.org/10.1128/MMBR.00034-06
  34. Selby, T.P., Lahm, G.P., Stevenson, T.M., Hughes, K.A., Cordova, D., Annan, I.B., Barry, J.D., Benner, E.A., Currie, M.J., Pahutski, T.F., 2013. Discovery of cyantraniliprole, a potent and selective anthranilic diamide ryanodine receptor activator with crossspectrum insecticidal activity. Bioorg. Med. Chem. Lett. 23, 6341-6345. https://doi.org/10.1016/j.bmcl.2013.09.076
  35. Sharma, S., Sood, A.K., Ghongade, D.S., 2022. Assessment of losses inflicted by the aphid, Myzus persicae (Sulzer) to sweet pepper under protected environment in north western Indian Himalayan region. Phytoparasitica 50, 51-62. https://doi.org/10.1007/s12600-021-00951-7
  36. Shin, W., Kim, G., Park, N., Kim, J., Cho, K., 2002. Effect of host plants on the development and reproduction of cotton caterpillar, Palpita indica (Saunder). Korean J. Appl. Entomol. 41, 269-273.
  37. Shipp, J.L., Wang, K., Binns, M.R., 2000. Economic injury levels for western flower thrips (Thysanoptera: Thripidae) on greenhouse cucumber. J. Econ. Entomol. 93, 1732-1740. https://doi.org/10.1603/0022-0493-93.6.1732
  38. Singh, K.S., Cordeiro, E.M.G., Troczka, B.J., Pym, A., Mackisack, J., Mathers, T.C., Duarte, A., Legeai, F., Robin, S., Bielza, P., Williamson, M.S., 2021. Global patterns in genomic diversity underpinning the evolution of insecticide resistance in the aphid crop pest Myzus persicae. Commun. Biol. 4, 847. https://doi.org/10.1038/s42003-021-02373-x
  39. Soderlund, D.M., 2012. Molecular mechanisms of pyrethroid insecticide neurotoxicity: recent advances. Arch. Toxicol. 86, 165-181. https://doi.org/10.1007/s00204-011-0726-x
  40. Sparks, T.C., Nauen, R., 2015. IRAC: Mode of action classification and insecticide resistance management. Pestic. Biochem. Physiol. 121, 122-128. https://doi.org/10.1016/j.pestbp.2014.11.014
  41. Statistics Korea, 2024. Vegetable production (fruits and vegetables) [Electronic data]. Agricultural Area Survey, Statistics Korea, Daejeon. https://kostat.go.kr (accessed on 1 May, 2026).
  42. Sun, W., Shahrajabian, M.H., Cheng, Q., 2020. Pyrethrum, an organic and natural pesticide. J. Biol. Environ. Sci. 14, 41-44.
  43. Suraweera, D.D., Groom, T., Taylor, P.W.J., Jayasinghe, C.S., Nicolas, M.E., 2017. Dynamics of flower, achene and trichome development governs the accumulation of pyrethrins in pyrethrum (Tanacetum cinerariifolium) under irrigated and dryland conditions. Ind. Crops Prod. 109, 123-133. https://doi.org/10.1016/j.indcrop.2017.07.042
  44. Wang, H., Peng, H., Li, W., Cheng, P., Gong, M., 2021. The toxins of Beauveria bassiana and the strategies to improve their virulence to insects. Front. Microbiol. 12, 705343. https://doi.org/10.3389/fmicb.2021.705343
  45. Yooboon, T., Pengsook, A., Ratwatthananon, A., Pluempanupat, W., Bullangpoti, V., 2019. A plant-based extract mixture for controlling Spodoptera litura (Lepidoptera: Noctuidae). Chem. Biol. Technol. Agric. 6, 5. https://doi.org/10.1186/s40538-019-0143-6