This study evaluates the performance of three AI instance segmentation models YOLOv8l-seg, Detectron2, and StarDist for microbubble diameter analysis. A ground truth dataset of 100 test images was prepared using Roboflow, and model performance was assessed using eight metrics: FPS, AP, Precision, Recall, PQ, RQ, SQ, and Boundary F1 score. YOLOv8l-seg achieved the highest FPS and Recall but tended to overestimate bubble diameters. Detectron2 showed the most balanced and accurate performance, with the smallest deviation from ground truth, while StarDist exhibited good segmentation accuracy but frequent false detections of small particles, leading to underestimation. Diameter analysis was conducted using an equivalent circle algorithm, with results compared via probability and cumulative density functions, scatter plots, and Bland-Altman analysis. Overall, Detectron2 demonstrated the highest reliability and accuracy, suggesting its strong potential for automated and high-volume micro-bubble diameter measurements.