The purpose of this study was to establish the origin of Korea tobacco, by homogeneity of the names and folk tales for the tobaccos introduced among three countries. According to the literatures that had written the origin concerning tobacco during the survival period of the Korean author, Korea tobacco came from Japan, for the first time, in 1611~1612. Six year s after the tobacco was introduced, in 1617~1618, tobacco seed also came from Japan. And 10 year safter the tobacco was introduced, in 1621~1622, there was no person that do not smoke. The Korea tobacco name, Dambago(淡婆姑), was the same as Japan tobacco name, Dambago(淡婆姑), but it was not the same as China tobacco name, Tambaku(淡巴菰). The Korea tobacco's folk tale, Dambago(淡婆姑) story, was the same as Japan tobacco's folk tale, Dambago(淡婆姑) story, but it was not the same as China tobacco's folk tale, Tambaku(淡巴菰) or Banhonhyang(返魂香) stories. This finding suggests that Korea tobacco may surely came from Japan, considering homogeneities of the names and the folk tales in the tobaccos introduced among three countries.

The purpose of this study is to classify the tobacco names in the world, to investigate the regional distribution of the classified type, and to show origin of names according to the type. The names of tobacco used in this study was 50. The type of tobacco names was classified by the presence or absence of nasal sound(morn) on the first syllable, the Number of syllable, and the structure of consonants and vowels of tobacco names. Type I (Dambago) has the nasal sound on the first syllable. The proportion of Type I(Dambago) was 28%. And the rest(Type I~Type V ; 72%) has no nasal sound. Type II(Tabaco) has three syllables, and its proportion was 20%. Type III(Tabac) has the two syllables and the structure of T+vowels+B+vowels. And its proportion was 30%. Type IV(Tutun) has the two syllables and the structure of T+vowels+T+vowels. And its proportion was 12%. Type V(Duhan) has the two syllables and the structure of D+vowels+H(V)+vowels. And its proportion was 10%. The world's most widely distributed type was Type I(Dambago). regional distribution of the world's tobacco names were clustered by the type. 72% of Type I(Dambago) was distributed in Asia. The etymology of Type I(Dambago) was only 14% Tambaku and the other is not yet known. The etymology of Type I(Dambago) seems to be derived from the Haitian Tambaku(meaning a tobacco pipe). 88% of Type II(Tabaco) and III(Tabac) were distributed in Europe. The etymology of Type II(Tabaco) and Type III(Tabac) were 84% Spanish "Tabaco". 100% of Type IV(Tutun) and V(Duhan) were distributed in Europe. The etymology of Type IV(Tutun) and Type V(Duhan) were 100% Turkish tutun and duhan, respectively. This finding suggests that the etymology of Type I(Dambago) is certainly may be Haitian "Tambaku(meaning a tobacco pipe)".

Urinary mutagenicity is widely recognized as a useful biomarker for the assessment of mutagen exposure level in human. In this study, we optimized the several parameters affecting the activity of Urinary mutagenicity using highly sensitive mutation test(microsuspension assay) instead of the conventional Ames test. First of all, we chose YG1024 as a highly sensitive strain from three str ains of Salmonella typhimurium(TA98, TA100, YG1024) using r epr esentative mutation substances, such as Benzo[a]pyrene, 2-Aminonaphthalene, 2-amino-3-methyl-9H-pyrido[2,3-b]indole($MeA{\alpha}C$) and cigarette total particulate matter(TPM). And we established the several kinds of test conditions such as number of bacter ia, concentr ation of metabolic activation system and incubation time for the most sensitive reaction. Also, we optimized efficient pre-treatment method using commercial C18 column. As a r esults, this method was shown a aver age of 94 % recovery value and 13 % relative standard deviation. When we compared the Urinary mutagenicity between several participants, we confirmed that compar ative measurements were possible for different levels of urine mutagenicity. In conclusion, the optimized highly sensitive mutation test to measure the Urinary mutagenicity may be useful in biological monitoring of mutagen exposure level.

Genome walking is a par ticular application for identifying sequences of unknown genomic regions adjacent to a known region. Many genome walking methods based on polymerase chain reaction (PCR) are available. Even if earlier techniques suffer from low reproducibility, inefficiency, and non-specificity, improved strategies have been developed. In this study, we present an alternative strategy: the genomic DNA is digested with restriction enzymes. After cytosine overhangs at 5' ends, the fragments are ligated to linker adaptor s had guanine overhang at 3' ends. Then nested PCR is performed. The improvements in this strategy focus on two points. The first is the C tailing method using Pfu polymerase instead of the A tailing method based on nontemplate-dependent terminal transferase activity of Taq polymerase. Therefore unintended modification of target DNA can be prevented without A tailing error. The second point is the use of C/G-specific ligation had advantage in the ligation efficiency compared with A/T-specific ligation. Therefore, the C-G linker PCR method increases ligation efficiency between digested genomic DNA and adaptor DNA. As a result, the quantity of target DNA to amplify by PCR is enriched. We successfully used G-C linker PCR to retrieve flanking regions bordering the phophinothricin resistance gene in genetically modified tobacco (GMO).