This study attempted to investigate vocal characteristics and differences in gender and voice classification among classical singers. Twenty-three female singers (M = 23.1 yrs, SD = 3.6 yrs, average 6.3 yrs singing experience, all classified as sopranos) and twenty male singers (M = 25.2 yrs, SD= 3.6 yrs, average 6. 3 yrs singing experience, 8 tenors, 12 baritones) were recruited to participate in the present study. Speaking fundamental frequency (FO), closed quotient (CQ), MPT (Maximum Phonation Time), breathing types, maximum inspiratory pressure (MIP), maximum expiratory pressure (MEP), and singers' formants were measured. In addition, vibratory patterns were observed using stroboscopy. Sfo, singing CQ, breathing types, formant frequency in singers' formants, MIP, MEP, and MPT were significantly different from gender to gender. Generally, singers' formants were observed in male singers and also the pattern of singers' formants was different between tenors and baritones. Lower singing CQ values were observed than speaking CQ values in the female singers (P<.001). Furthermore, MEP, MIP, and singing CQ were significantly lower for female singers than for males singers (P<.001). MPT and speaking FO, however, were not significantly different between tenors and baritones.