• Title/Summary/Keyword: 간보호

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A Study on the Antilipidperoxidative Effects of Brazilin and Hematoxylin(I) (천연색소 Brazilin 및 Hematoxylin의 항지질 과산화활성에 관한 연구 (I))

  • 문창규;하배진;이수환;목명수
    • Journal of Food Hygiene and Safety
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    • v.2 no.1
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    • pp.35-40
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    • 1987
  • The antilipidperoxidative effects of Brazilin and Hematoxylin were investigated at the levels of liver~total homogenates, -microsomal fraction, -mitochondrial fraction and the sera of SD-rats intoxicated with $CCl_4$ and ethanol. Both natural dyes markedly inhibited the lipidperoxidation induced by $CCl_4$ and ethanol. Brazilin and Hematoxylin showed the inhibitory effects on the both enzymatic (NADPH-dependent) and nonenzymatic (Ascorbate-induced) lipidperoxidation pathways. but it is supposed that the antilipidperoxidative powers of them mainly result from the inhibition of the nonenzymatic lipidperoxidation.

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Effect of Rheum undulatum Linne extract and Glycyrriza uralensis Fischer extract against arachidonic acid and iron-induced oxidative stress in HepG2 cell and CCl4-induced liver injury in mice (대황과 감초 병용의 항산화 및 간보호효과)

  • Lee, Eun Hye;Baek, Su Youn;Kim, Kwang-Youn;Lee, Seul-Gi;Kim, Sang Chan;Lee, Hyeong Sik;Kim, Young Woo
    • Herbal Formula Science
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    • v.24 no.3
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    • pp.163-174
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    • 2016
  • Objectives : Rheum undulatum Linne and Glycyrriza uralensis Fischer are widely used herbal medicine. In this study, anti-oxidant and liver protective effects of R. undunlatum extract (RUE) and G. uralensis extract (GUE) were investigated in HepG2 cells, respectively. Oxidative stress and liver fibrosis were induced by arachidonic acid (AA) and iron, and CCl4.Methods : MTT assay was assessed for cell viability, and immunoblotting analysis was performed to detect expression of apoptosis related proteins. In addition, reactive oxygen species (ROS) and mitochondrial dysfunction were measured. In vivo, BALB/c mouse were orally administrated with the aqueous extract of 10 mg/kg RUE and 100 mg/kg GUE for 3 days and then, injected with CCl4 0.5 ml/kg body weight to induce acute liver damage. Serum ALT level was measured, and histological change was observed in Harris's hematoxylin and eosin stainResults : RUE and GUE pre-treatment increased relative cell viability in concentration dependent manner and altered the expression levels of apoptosis-related proteins such as procaspase 3, PARP and Bcl-xL. RUE and GUE also inhibited the mitochondrial dysfunction and excessive reactive oxygen species (ROS) production induced by AA and iron. In addition, RUE and GUE activated liver kinase B1 (LKB1), by increasing phosphorylation. Moreover, RUE and GUE treatment decreased liver injuries induced by CCl4, as evidenced by decreases in histological liver damage as well as serum alanine amino transferase (ALT) level.Conclusions : These data suggest that RUE and GUE has anti-oxidant and liver protective effects against AA and iron-induced oxidative stress and CCl4-induced liver injury.

Protective Effect of Tongyuhwalhyeol-tang on Liver Injury in Thioacetamide-induced Rat (Thioacetamide 유발 간손상모델에서 통규활혈탕의 간보호효과)

  • Kim, Kyeong Jo;Shin, Mi-Rae;Kim, Soo Hyun;Kim, Su Ji;Lee, Ah Reum;Kwon, Ojun;Roh, Seong-Soo
    • The Korea Journal of Herbology
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    • v.33 no.1
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    • pp.37-46
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    • 2018
  • Objectives : Liver disease is an inflammatory reaction caused by oxidative stress, viral, alcohol, and drug properties. Inflammatory reaction causes hepatitis and chronic hepatitis is persistent, it progresses to liver fibrogenesis and liver cancer. The aim of this study was to confirm the hepatoprotective effect of Tongyuhwalhyeol-tang(Tongqiaohuoxue Decoction) (TH) and Gamtongyuhawlhyeol-tang(GTH) in TAA-induced liver injury animal model. Methods : The antioxidant activities were evaluated through in vitro experiments, such as 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and 2, 2'-azinobis-3-ethyl-benzothiazoline-6-sulfonic acid (ABTS) radical scavenging assays, total polyphenol and total flavonoid content measurement. To confirm the liver protective effect, induced by Thioacetamide (TAA) for 3 days injection at 200 mg/kg rats. TH and GTH were treated 3 days at 200 mg/kg/day. The changes of reactive oxygen species (ROS), peroxynitrite ($ONOO^-$), alanine aminotransferanse (ALT) and aspartate aminotransferase (AST) in serum were analyzed after experiment. Also, expression of anti-inflammation, anti-oxidant related proteins were investigated by western blot analysis. Results : TH was inhibited the antioxidant activities. In the TAA-induced rat, TH decreased ROS, $ONOO^-$, ALT, AST level in serum. Inflammation related protein expressions increased in TAA-induced rat compared to normal rat. However, TH group inhibited the down expression of these proteins. Also, anti-oxidant related protein expressions increased in TH group compared TAA-induced rat. Conclusion : Therefor, these results suggested that TH provided hepatoprotective effects on the hepatic injury leading to the reduction of inflammatory response. In addition, the effect of TH was superior to that of GTH.

A Systematic Review of Group Programs for Community-dwelling Elderly (지역사회 거주 노인 대상의 그룹 프로그램에 대한 체계적 고찰)

  • Jeong, Eun-Hwa;Ju, Yumi
    • Therapeutic Science for Rehabilitation
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    • v.10 no.2
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    • pp.23-36
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    • 2021
  • Objective : The purpose of this study was to analyze group programs for the elderly living in communities and to provide basic data for applying effective occupational therapy programs for the elderly in the community. Methods : From January 2009 to December 2019, articles on group programs for the elderly in the community were searched for in the NDSL, DBPia, Riss, and PubMed databases. Based on the inclusion and exclusion criteria, a total 16 out of the 147 identified studies were selected and analyzed. This review analyzed the characteristics of the participants, contents of the group program, session and duration of the intervention, outcome measures, and effect of the group program. Results : Single group pre-post test studies with evidence level III were most common (8, 50.0%), and five (31.3%) of the studies were conducted on the community-dwelling elderly. Across all the 16 studies, there were 10 types of group programs, and the most frequently used was an exercise group program (25.0%). According to the analysis of the group program period, sessions and time, the intervention period was most commonly 12 weeks (37.5%), with 8 sessions or 12 sessions (25.0%). The intervention time in the group program was most commonly 60 minutes (43.8%). Conclusion : This study can be used as a basis for the development of effective group programs for the elderly and patients with dementia in community-based long-term care services.

Hepatoprotective effect of Paeoniae radix via Nrf2 activation (Nrf2 활성화(活性化)를 통한 작약(芍藥)의 간보호효과(肝保護效果))

  • Lee, Soo Hwan;Jung, Ji Yun;Park, Sang Mi;Jegal, Kyung Hwan;Byun, Sung Hui;Cho, Il Je;Kim, Sang Chan;Kim, Kwang Joong;Kim, Young Woo
    • The Korea Journal of Herbology
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    • v.31 no.1
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    • pp.33-40
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    • 2016
  • Objectives : Liver is one of the largest organs in the human, and has a function of detoxification and energy sensing to prevent severe disease. Paeoniae radix has been used to treat a variety of liver diseases such as hepatitis and chronic hepatic failure. Although P. radix has been used as an medicinal herb for a long time, the effects of P. radix on severe oxidative stress and its action mechanism on the liver was not clearly verified.Methods : This study investigated the protective effects of P. radix extract (PRE), and the underlying mechanism of its action in the liver. tert-butyl hydroperoxide (t-BHP) and carbon tetrachlroride (CCl4) were used to induce oxidative stress in the HepG2 hepatocyte cell line and Sprague-Dawley rats, respectively.Results : t-BHP significantly induced cell death and ROS production in HepG2 cell, as indicated by MTT and FACS analysis. However, pretreatment of PRE inhibited a decrease in cell viability and H2O2 production in the HepG2 cells. PRE also blocked the ability of t-BHP to damage in mitochondrial membrane transition. More importantly, PRE induced Nrf2 activation and antioxidant Phase II enzyme, which may have a role in the effects of PRE. In mice, PRE inhibited the liver damage induced by CCl4.Conclusions : PRE inhibited oxidative stress and hepatic damages as mediated with Nrf2 activation. This study unveil, in part, the effect and mechanism of old medicinal herb, P. radix.

Analysis on the Use of Welfare Services of Elderly Long-term Care Grade Accredited and Unidentified (노인장기요양 등급인정자와 등급 외자의 지역사회복지서비스 이용 실태분석)

  • Lee, Yong-Jae;Kim, Hyo-Sim
    • Journal of Digital Convergence
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    • v.17 no.11
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    • pp.29-37
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    • 2019
  • Elderly people want to live in the community even if they are in poor health. However, there is no integrated care support system suitable for the health and functional status of the elderly. So the elderly are choosing living facilities. The purpose of this study is to explore exploring whether the elderly are applying for a long-term care certification. First, mild geriatric patients were mainly using home care services such as visitation care. However, some of the milder elderly were enrolled in nursing homes. And the service that does not fit the functional status of the elderly is used. Second, it is concentrated on the use of visiting care services. Third, elderly people outside the class did not receive sufficient help for daily life, and the use of community welfare services such as the elderly welfare center was low. As a result, long-term care admitters are not able to continue to live in the community even though their health and functioning status is mild, and elderly people out of grade are unable to properly use the necessary community care services. The condition is likely to deteriorate.

The Structural Relationship among Job Stress, Human Rights Behavior, Social Support and Job Satisfaction (노인장기요양시설 종사자들의 직무스트레스와 인권옹호행동, 직무만족의 구조적 관계: 사회적 지지의 매개효과를 중심으로)

  • Kim, Keunhong;Song, Jiwon
    • 한국노년학
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    • v.37 no.3
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    • pp.747-762
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    • 2017
  • Recently, as the number of patients with dementia using nursing homes increases, the interest about the quality of care services is increasing. Job satisfaction of caregivers is very important factor in the quality of care service. In addition, as perception of human rights is heightened, human rights behavior in nursing homes is also recognized as an important factor. The purpose of this study is to investigate the structural relationship between job satisfaction and human rights behavior, job stress, and social support of dementia workers who have significant influence on quality of care service. For this purpose, 210 questionnaires were collected from 300 dementia workers working in the elderly nursing home, day night care center, and domiciliary visit care center in Gyeonggi province. After excluding 13 questionnaires, the questionnaires were analysed by AMOS and SPSS 21.0. The results of the analysis are following. It was found that job stress significantly affects the social support directly (-.276). Also, social support significantly affects job satisfaction (.315). On the other hand, human rights advocacy has a direct effect on job satisfaction (.175) and an indirect effect (.102), showed a total effect of (.277). This shows that social support partially mediates human rights advocacy and job satisfaction. Job stress had a direct effect on job satisfaction(-.217) and an indirect effect(-.095), indicating a total effect of (-.312), and social support partially mediated job stress and job satisfaction Could know. The results of this study suggest that in order to improve the job satisfaction of the dementia workers, it is necessary to increase the human rights advocacy behavior and reduce the job stress in an environment that recognizes the social support.

Application of Lee Silverman Voice Treatment-BIG(LSVT-BIG) Intervention to Improve Motor Functions and Quality of Life in People With Parkinson Disease (파킨슨병 환자에게 Lee Silverman Voice Treatment-BIG(LSVT-BIG) 프로그램의 적용이 운동기능과 삶의 질에 미치는 효과)

  • Park, Kang-Hyun;Kim, Jae-Hwan;Jang, Jong-Sik
    • Therapeutic Science for Rehabilitation
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    • v.8 no.1
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    • pp.73-84
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    • 2019
  • Objective: The purpose of this study is to compare the effect of Lee Silverman Voice Treatment-BIG(LSVT-BIG) intervention which consisted of standardized exercise programs and occupation-based activities for people with Parkinson Disease(PD) on motor functions and quality of life Methods: This study applied a one group pretest-posttest design. The experiment was divided into two parts: pre intervention and post intervention period. Before and after LSVT-BIG intervention, Unified Parkinson's Disease Rating Scale(UPDRS), Time up and go(TUG), Parkinson's Disease Questionnaire-39(PDQ-39) were used to measure the participants' motor functions and quality of life. Based on the LSVT-BIG protocol, three participants received 16 one-hour sessions over 4 weeks by a certified occupational therapist. The results were analyzed by using SPSS. Results: There were improvements in UPDRS and TUG. Additionally, PDQ-39 scores decreased in all participants, which means that their quality of life was improved. Conclusions: The study demonstrated positive effects of LSVT-BIG intervention on motor functions and quality of life of patients with PD.

Anti-obesity Effect of Ethanol Extracts from Silkworm (Bombyx mori) Pupae Powder Fermented with Cordyceps militaris in the Primary Adipocytes and High Fat Diet-induced Obesity Model Mice (일차 지방세포와 고지방식이로 유발한 비만모델동물에서 Cordyceps militaris로 발효시킨 누에 (Bombyx mori) 가루 에탄올 추출물의 항비만 효과)

  • Kim, Ji Eun;Lee, Mi Rim;Choi, Jun Young;Park, Jin Ju;Kim, Hye Ryeong;Song, Bo Ram;Choi, Young Whan;Kim, Kyung Mi;Hwang, Dae Youn
    • Journal of Life Science
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    • v.28 no.7
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    • pp.786-794
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    • 2018
  • Silkworm (Bombyx mori) pupae have been widely worked in wound dressing, hepatoprotective activity, antigenotoxicity, control of glucose level and anti-cancer activity. To investigate the anti-obesity activity of ethanol extract of silkworm pupae powder fermented with Cordyceps militaris (ESfC), the free glycerol release and cAMP concentration as well as fat accumulation were measured in the primary adipocytes of SD (Sprague Dawley) rats and high fat diet (HFD)-treated C57BL/6 mice treated with 12 weeks. Firstly, the presence of the cordycepin with lipid lowering effect was confirmed in ESfC using HPLC analysis. The level of free glycerol and cAMP concentration was significantly increased in the primary adipocytes treated with high dose of ESfC ($400{\mu}g/ml$) although these levels were consistently maintained in other dose ESfC treated groups. In HFD-induced obesity model, the increased fat weight and size of adipocytes in HFD+Vehicle treated group was recovered in HFD+ESfC treated group. Also, the liver weight and the number of lipid droplets were higher in HFD+Vehicle treated group than No treated group. But, this level was significantly decreased in HFD+ESfC treated group compared with HFD+Vehicle treated group. Furthermore, a similar recovery was detected on the phosphorylation of periliphin and HSL, and ATGL expression. Overall, the results of the present study provide some scientific evidences that ESfC can stimulate lipolysis in primary adipocytes and prevent fat accumulation in HFD-treated obesity model, and therefore have the potential for use as anti-obesity agents to treat obese patient.

Hepatic Protective Effect and Single-dose Toxicity Study of Water Extract of Cordyceps militaris Grown upon Protaetia dreujtarsis (굼벵이 유래 밀리타리스 동충하초 열수추출물의 간기능개선 효과 및 단회독성 평가)

  • Jo, Wol-Soon;Nam, Byung-Hyouk;Oh, Su-Jung;Choi, Yoo-Jin;Kang, Eun-Young;Hong, Sook-Hee;Lee, Sang-Ho;Jeong, Min-Ho
    • Korean Journal of Food Science and Technology
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    • v.40 no.1
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    • pp.106-110
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    • 2008
  • This study was designed to evaluate the single dose toxicity and the protective effect of water extract of Cordyceps militaris grown upon Protaetia dreujtarsis (CMPD extract) on liver damage on carbon tetrachloride ($CCl_4$)- induced acute hepatotoxicity in Sprague-Dawley (SD) rats. The CMPD extract was once administered orally to both sexes of rats at dose of 2,000, 1,000 and 500 mg/kg body weight, the recommended maximum limit dose for acute toxicity. Neither significant toxic signs nor death was observed during the observation period. These results indicate that $LD_{50}$(lethal dose of 50%) of CMPD extract is greater than 2,000 mg/kg body weight in SD rats. To investigate also the effect of hepatoprotection of CMPD extract, SD rats were orally treated with CMPD extract (50, 25 and 12.5 mg/kg body weight) or silymarin (25 mg/kg body weight) before and after administration of $CCl_4$ (2 mL/kg body weight, 20% $CCl_4$ in olive oil). Treatment with CMPD extract or silymarin could decrease the GPT (glutamic-pyruvic transaminase) and GOT (glutamic-oxaloacetic transaminase) levels in serum when compared with $CCl_4$-treated group. Therefore, the results of this study show that CMPD extract can be proposed to protect the liver against $CCl_4$-induced hepatic damage in rats.