• Title/Summary/Keyword: 간보호

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Antioxidative and Hepatoprotective Effect of Compounds Isolated from Galla Rhois(Rhus javanica Linne) (오배자 성분의 항산화 및 간보호 효과)

  • Cha, Bae-Cheon;Lee, Sung-Bae;Rhim, Tae-Jin;Lee, Kwang-Hoe
    • Korean Journal of Medicinal Crop Science
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    • v.8 no.2
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    • pp.157-164
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    • 2000
  • The major components were isolated from the n-hexane, EtOAc and BuOH extract of Galla Rhois(Rhus Javanica Linne). Their structures were characterized as syringic acid, gallic acid methylester, protocatechuic acid, gallic acid and 1, 2, 3, 4, $6-penta-O-galloyl-{\beta}-D-glucose$. This study was carried out to investigate the biological activities of isolated compounds. Five compounds were tested for hepatoprotective effects on CCl4-induced cytotoxicity in primary cultured rat hepatocytes and antioxidative effect on Ferric-Thiocyanate method and TBA method. As a result, isolated five compounds showed stronger antioxidative activity than tocopherol, and the antioxidative activity of gallic acid methylester, protocatechuic acid and syringic acid were similar to that of BHA on Ferric-Thiocyanate method. Specially 1, 2, 3, 4, $6-penta-O-galloyl-{\beta}-D-glucose$ showed stronger effect of lipid-peroxidation inhibition than BHA. Gallic acid appeared stronger inhibitory effect of malondialdehyde on TBA method. Hepatoprotective effect of 1, 2, 3, 4, $6-penta-O-galloyl -{\beta}-D-glucose$ was similar or even higher than that of glycyrrhizin on primary cultured rat hepatocyte cytotoxicity.

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The Classification, Origin, Collection, Determination of Activity, Purification, Production, and Application of Agarases (Agarase의 분류, 기원, 확보, 활성파악, 분리정제, 생산 및 응용)

  • Lee, Dong-Geun;Lee, Sang-Hyeon
    • Journal of Life Science
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    • v.22 no.2
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    • pp.266-280
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    • 2012
  • Agar is a cell wall component of macro red algae that can be hydrolyzed by agarase. Agarases are classified into ${\alpha}$-agarase (E.C. 3.2.1.158) and ${\beta}$-agarase (E.C. 3.2.1.81), in accordance with their cleavage pattern, and can be grouped in the glycoside hydrolase (GH)-16, -58, -86, -96, and -118 family according to the amino acid sequences of the proteins. Many agarases and/or their genes have been detected, isolated, and recombinantly expressed from bacteria, and metagenomes have their origins in sea and terrestrial environments. Products of agarases, agarooligosaccharides and neoagarooligosaccharides, represent wide functions such as antitumor, immune stimulation, antioxidation, prebiotic, hepa-protective, antibacterial, whitening, and moisturizing effects; hence, broad applications would be possible in the food industry, cosmetics, and medical fields. In addition, agarases are also used as a tool enzyme for research. This paper reviews the sources, purifications and detection methods, and application fields of agarases. The role of agarases in agar metabolism and the function of their enzymatic products are also surveyed.

Evaluation of antitumor. hepatoprotective and antimutagenic potentials of Phellinus gilvus (Phellinus gilvus의 항암활성, 간보호 및 항돌연변이성에 대한 평가)

  • Kang, Eun-Hee;Kim, Kil-Soo;Park, Seung-Chun
    • Korean Journal of Veterinary Research
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    • v.48 no.1
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    • pp.17-26
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    • 2008
  • This study was carried out to evaluate the antitumor, hepatoprotective and antimutagenic activities on hot water extract of Phellinus gilvus (PGE). Growth of tumor in mice that were orally given $0.25,0.5,1.0,2.0g\;kg^{-1}$ dose of PGE was inhibited in a dose-dependent manner (p < 0.05). The hepatoprotective effect of PGE in the carbon tetrachloride ($CCl_4$)-intoxicated rats was studied. In $CCl_4$ + PGE group, PGE was orally administered with 100 mg/kg/day dose 7 days before the treatment of $CCl_4$. The serum activity of aspartate aminotransferase and alanine aminotransferase in $CCl_4$ + PGE group were decreased at a rate of 59.6% and 54.1% compared with those in $CCl_4$ group, respectively (p < 0.05). Also, total cholesterol and triglyceride in $CCl_4$ + PGE group were significantly decreased at a rate of 90% and 73.6% compared with those in $CCl_4$ group (p < 0.05). In the Ames test, we confirmed PGE doesn't have any activity as a mutant, and PGE showed inhibitory effect against mutagenesis induced by 2-amino fluride and sodium azide in Salmonella typhimurium TA98, TA100 and TA1535 in a dose-dependent manner. From the above results, we may suggest that PGE might have useful as a material for functional food and/or animal pharmaceutics.

Hepatoprotective Activities of Curcumin, Demethoxycurcumin and Bisdemethoxycurcumin (Curcumin, demethoxycurcumin 및 bisdemethoxycurcumin의 간보호 작용)

  • Cheon, Ho-Jun;Park, Jin-Goo;Kim, Yeong-Shik;Kang, Sam-Sik;Chi, Xing-Fu;Lee, Jung-Joon;Lee, Sun-Mee
    • Korean Journal of Pharmacognosy
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    • v.38 no.2 s.149
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    • pp.139-147
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    • 2007
  • The aim of this study was to investigate the protective activity of curcumin, demethoxycurcumin and bisdemethoxycurcumin, isolated from Curcuma longa Linne, on hepatocyte injury induced by carbon tetrachloride (CCl$_4$,10 mM), t-butyl hydroperoxide (TBH, 0.5 mM) and D-galactosamine (GaIN,30 mM). Primary cultures of rat hepatocyte (18 h culture) were treated with CCl$_4$, TBH or GaIN and various concentrations (0.1, 1, 10 and 100 ${\mu}$M) of curcumin, demethoxycurcumin and bisdemethoxycurcumin CCl$_4$ significantly increased the levels of lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate aminotransferase (AST). The increases in LDH, ALT and AST levels were inhibited by curcumin. Demethoxycurcumin and bisdemethoxycurcumin decreased the levels of AST. Curcumin inhibited the increases in ALT and AST levels induced by TBH. The increased levels of LDH, ALT and AST induced by TBH were inhibited by bisdemethoxycurcumin. GaIN markedly increased the levels of LDH, ALT and AST. These increases were significantly inhibited by bisdemethoxycurcumin. The increase in AST level was inhibited by curcumin. These results suggest that curcumin and bisdemethoxycurcumin have potent hepatoprotective activities.

Hepatoprotective Effect of Bark of Phellodendron amurense RUPR. on Liver Damage Induced by Carbon Tetrachloride (사염화탄소에 의한 간손상에 대한 황백의 간보호 효과 연구)

  • Kwak, Chang-Geun;Kim, Jae-Eun;Choi, Dall-Yeong;Jeong, Han-Sol;Shin, Sang-Woo;Joo, Myoung-Su;Ha, Ki-Tae
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.25 no.4
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    • pp.620-627
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    • 2011
  • We evaluated the hepatoprotective activity of crude hot-water extracts of the traditional Korean medicinal herb, Phellodendri Cortex (PC; Bark of Phellodendron amurense RUPR.), in an experimental model of hepatic damage induced by carbon tetrachloride ($CCl_4$). The serum marker of liver damage, sGOT, sGPT and sALP, were significantly decreased in the liver of the PC treated rats, compared with that of $CCl_4$ treated group. The histological observation of liver section of rats, showed the same protective effect of PC treatment. And the protective activity of PC was more significant in the post-treated group than pre-treated group. The significant decrease of malodialdehyde and increase of glutathion, catalase activity were observed in the liver homogenate of PC treated rats. Based on these findings, it is suggested that PC has potent hepatoprotective effects and the mechanism of the protection may be related to antioxidation pathways.

Protective Effect of Spatholobi Caulis in Thioacetamide induced Acute Liver Injury of Rat (Thioacetamide로 유발한 간손상 모델에서 계혈등(鷄血藤)의 간보호 효과)

  • Oh, Min Hyuck;Shin, Mi-Rae;Roh, Seong-Soo
    • The Korea Journal of Herbology
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    • v.36 no.2
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    • pp.31-42
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    • 2021
  • Objectives : This study was undertaken to investigate the hepatoprotective effect of Spatholobi Caulis water extract (SC) to thioacetamide (TAA)-induced acute liver injury (ALI) in rats. Methods : The rats were injected intraperitoneally with TAA (200 mg/kg body weight) and orally administered SC (100 or 200 mg/kg b.w.) daily for 3 days. Liver biomarkers were assessed by serum glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), and ammonia levels. Malondialdehyde (MDA) was measured both serum and liver tissue. In addition, nicotinamide adenine dinucleotide phosphate (NADPH) oxidase, anti-oxidant, and inflammation-related proteins were investigated by western blot analysis. Histological examination further confirmed though hematoxylin and eosin stain. Results : The SC treatment reduced liver function markers like GOT and GPT and also remarkably decreased ammonia level. Moreover, the elevated MDA level in TAA-induced group was significantly reduced by SC treatment. NADPH oxidase expression associated with oxidative stress including NOX2, NOX4, and p47phox markedly inhibited by SC administration. SC treatment exerted anti-oxidant effect through the increase of anti-oxidant enzyme including superoxide dismutase (SOD), Catalase, and heme oxygenase-1 (HO-1). The protein expressions of inflammatory cytokines such as tumor necrosis factor-�� (TNF-��), IL-6, and IL-1�� induced by nuclear factor-kappa B (NF-��B) activation were modulated through blocking the phosphorylation of inhibitor of nuclear factor ��B�� (I��B)��. SC treatment also improved histological alterations. Conclusion : These findings suggested that SC administration may be a potential candidate for the prevention or treatment of ALI.

Antioxidative and Hepatoprotective Activity of Colured-Scented and Korean Native Rice Varieties Based on Different Layers (특수 유색미, 향류미 및 한국 재래종 벼 종자의 층위별 항산화 및 간보호 활성)

  • 박희준;곽태순;정원태;최종원
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.28 no.1
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    • pp.191-198
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    • 1999
  • Fifteen varieties of Oryza sativa mainly including those of Korean native rice were exactly cutted into three layers such as L1 layer(the outer part of 92% from rice center), L2 layer(the part of 81∼91% from the rice center) and L3 layer(the inner part of 80% from the rice center) We biologically evaluated the antioxidative effect on the every layer(L1, L2 and L3) of all the varieties, by observing malondialdehyde (MDA) produced by galactosamine in the mouse. L1 layer of some varieties showed significant antiox idative effect, while L2 and L3 layer didn't show the activity. It was also suggested that coloured rice(Suwon 425 and Sanggaehangbyeolna) showed stronger activity than other general rices, probably due to high contents of anthocyanins. Successively, we evaluated antihepatotoxic effect, based on the determination of serum ALT and AST activity. Some varieties of only L1 layer, except for L2 and L3 layer, significantly decreased the serum ALT and AST activity. This finding indicate that oral diet of some raw rice are able to protect hepatotoxicities. Among all the samples tested, L1 layer of Suwon 425 showed the strongest antihepatotoxic effect. From quantitative analysis on ferulic acid derivatives, it was found that the more it enters from the surface into the rice center, the more those secondary metabolites contents were highly reduced. These findings above suggested that Suwon 425 could be a promising candidate for the development of health rice food.

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Hepatoprotective and Blood Alcohol Lowering Effects of Fruit Peduncle Extract of Hovenia dulcis var. Koreana in the In Vitro and in Vivo Animal Models (헛개나무(Hovenia dulcis var. koreana) 과병 열수추출분획물의 간보호 및 혈중 알콜 저하 작용)

  • 나천수;정남철;양규환;김세현;정하숙;동미숙
    • YAKHAK HOEJI
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    • v.48 no.1
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    • pp.34-40
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    • 2004
  • Hovenia dulcis which is distributed in Korea, China and Japan is known to show hepatoprotctive effect and reduce the acute alcohol toxicity. In this study, the hepatoprotective effect against the chemically induced experimental liver injury models and lowering effect of blood alcohol level in animal models acutely administered alcohol by the peduncle extracts of Hovenia dulcis var. koreana were investigated. HdfHW-1, and HdfM-1 which are the extracts of fruit peduncles and young branches with hot water or 70% methanol and followed with 100% methanol, were significantly reduced the $CCl_4$ or D-galactosamine/LPS induced damage in sliced liver. The hot water or methanol extracts of fruit peduncle protected dose-dependently against $CCl_4$ induced toxicity in primary hepatocyte culture and particularly, the amount of LDH release was reduced to the control level at 500 $\mu\textrm{g}$/ml of hot water extracts. HdfHW-1 also decreased the hepatotoxicity induced by $CCl_4$ in rats. The active components of HdfHW-1 seemed to be high molecular weights because 0.2 M NaCl HdfHW-1 fraction was the most effective among NaCl fractions of HdfHW-1 eluted with various concentrations of NaCl on DEAE 650C column chromatography. HdfM and HdfHW were significantly reduced the levels of blood alcohol in rats and mice administered 40% alcohol. These results indicated that the hot water or methanol extracts of fruit peduncle of Hovenia dulcis var. koreana have hepatoprotective effect and may be reduce alcohol toxicity.

Antioxidant, Antiinflammation and Hepatoprotective activity of Schizandrae Fructus processed with differenciated steaming number (증숙 오미자의 항산화, 항염증 및 간보호 효과 비교 연구)

  • Choo, Byung Kil;Chung, Ki Hun;Seo, Young-Bae;Roh, Seong-Soo
    • The Korea Journal of Herbology
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    • v.28 no.2
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    • pp.83-92
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    • 2013
  • Objectives : We investigated differances of physiological functionalities in the steamed Schisandrae Fructus. Methods : The samples were extracts of dryed schisandrae fructus without steaming process (S0), extracts of schisandrae fructus with three times steamed (S3), extracts of schisandrae fructus with five times steamed (S5) and extracts of schisandrae fructus with seven times steamed (S7). We analyzed contents of schisandrin, gomisin, total polyphenol and flavonoid and antioxidant activities. We researched antiinflammation effects for Raw264.7 cells. To evaluate liver protective activity, we measured AST, ALT and gamma-GTP in serum of alcoholic mice. Results : As the steaming number of schisandrae fructus increase, the contents of schisandrin and gomisin were more increased. The contents of total polyphenol of S5 and S7 were significantly increased compared to that of S0. DPPH free radical scavenging activities of S5 and S7 were significantly increased compared to that of S0, ABST radical scavenging activities of S3 and S5 were significantly increased compared to that of S0 in vitro. The NO production of all sample was significantly decreased compared to control, PGE2 release of S3, S5 and S7 were significantly decreased compared to control. IL-$1{\beta}$ release of S5 and S7 were significantly decreased. AST, ALT and gamma-GTP of S3, S5 and S7 were significantly decreased compared to control. Conclusions : We think that extracts of schisandrae fructus with steaming process may have more potential efficacy than a schisandrae fructus without steaming process.

Hepatoprotective Activities of Rosa davurica Root Extract in Rats Intoxicated with Bromobenzene (브로모벤젠으로 유도된 간독성 흰쥐에서 생열귀나무 뿌리의 간보호활성)

  • Park, Jong-Cheol;Hur, Jong-Moon;Hwang, Young-Hee;Choi, Myeong-Rak;Kim, Suk-Nam;Choi, Jong-Won
    • Journal of Life Science
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    • v.13 no.2
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    • pp.230-235
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    • 2003
  • To investigate hepatoprotective activities of the root extract of Rosa davurica, the activities of hepatic enzymes, aminopyrine N-demethylase, aniline hydroxylase, glutathione S-transferase and epoxide hydrolase in rats intoxicated with bromobenzene were studied. Pretreatment with the methanol extract from the roots of Rosa davurica did not show any significant effects on the increases of the activities of aminopyrine N-demethylase and aniline hydroxylase, enzymes forming toxic epoxide by bromobenzene. There was no change in glutathione S-transferase activity by Rosa davurica. However, the activity of epoxide hydrolase, and epoxide-removing enzyme, was increased 33% by the administration of 500 mg/kg of the methanol extract. From the results, the protection of Rosa davurica against bromobenzene-induced hepatotoxicity is thought to be via enhancing the activity of epoxide hydrolase, an enzyme removing toxic epoxide rather than through epoxide-producing system.