• Title, Summary, Keyword: 유충

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Comparative Analysis of Nutrients and Hazardous Substances in Zophobas atratus Larvae (탈지 전, 후 아메리카왕거저리(Zophobas atratus) 유충의 영양성분 및 유해물질 비교분석)

  • Kim, Sun Young;Kim, Hong Geun;Ko, Hyeon-Jin;Kim, Mi Ae;Kim, In Woo;Seo, Minchul;Lee, Joon Ha;Lee, Hwa Jeong;Baek, Minhee;Hwang, Jae Sam;Yoon, Hyung Joo
    • Journal of Life Science
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    • v.29 no.12
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    • pp.1378-1385
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    • 2019
  • The nutritional composition and optimal eating stage of the super mealworm, Zophobas atratus (Coleoptera : Tenebrionidae), were investigated to explore its use as a food ingredient. It was determined that 10th instar larvae were most suitable for eating in terms of nutritional value as well as economic aspects. To improve the quality of powder production, the nutritional value of 10th instar larvae before and after degreasing was analyzed. After drying the larvae powder, crude protein was the most abundant nutrient both before (52.3%) and after (60.6%) degreasing while crude fat measured 36.3% and 21.7% before and after degreasing, respectively. In terms of essential amino acids, leucine levels were highest and 1.3 times greater after degreasing (4.5%) than before (3.5%). Oleic acid, the highest unsaturated fatty acid in larvae, was 31.7% after degreasing which was 1.1 times higher than before (33.2%). Among various major minerals, potassium was most abundant and 1.4 times higher after degreasing (1267.0 mg/100 g) than before (879.3 mg/100 g). Harmful substances were 1.3 to 2.0 times lower in the degreased larvae, although mercury or pathogenic bacteria were not detected in either group. We therefore conclude that degreased Z. atratus larvae are more suitable for eating than before degreasing.

Enzyme-histochemical Studies of LDH and SDH on Developing Spirometro erinocei. (Spirometra erinacei의 발육에 따른 LDH와 SDH의 조직화학적 연구)

  • 곽기훈;김창환
    • The Korean Journal of Zoology
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    • v.35 no.3
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    • pp.362-371
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    • 1992
  • Spiromeiru erinocer익 유충(sparganum)을 전이숙주(paratenic host)인 흰쥐와 종숙주인 고양이에 감염시켜 발육단계에 따라 회수해서 유충과 성충조직의 LDH(lactate dehydrogenase)와 SDH(succinate dehydrogenase)의 분포상 및 동위효소 유형을 효소조직화학적 방법과 전기영동법으로 조사하였다. 첫째, 유충조직에서의 LDH분포는 발육과정에 따른 차이는 없었으며 표피층과 실질근층에 비해 표피근층이 많이 분포하였다. 성체조직에서도 표피근층에 많이 분포하였는데 특히 성체의 표피층에서의 LOH 활성은 수태편절에서 상당히 많은 활성을 보인 반면 다른 편절의 표피층에서는 LDH의 활성이 없었다. 둘째, SDH의 조직분포는 유충과 성충 모두 표피근층에 많이 분포하58h으며 유충에서의 발육 후기는 발육초기보다 SDH의 분포량이 증가하였다. 셋째, LDH등위효소 유형은 유충과 성충에서 2개씩 나타났는데 유충에서는 M.W. 140 Kd가, 성충에서는 56 Kd가 주분획(major band)이었다. 넷째, SDH등위효소는 유충에서 1개(132 Kd), 성충에서 2개(132 Kd, 45 Kd)가'분리되었으며 성충에서 활성이 높게 나타난 band는 45 Kd인 단백질이었다.

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Pupation Site Selection Behavior of Plodia interpunctella (Lepidoptera: Pyralidae) in Response to Its Parasitoid, Bracon hebetor (Hymenoptera: Braconidae) (보리나방살이고치벌에 대한 행동 반응으로서의 화랑곡나방 유충의 용화장소 선택)

  • Shin, Jae-Hyub;Nam, Young-Woo;Ryoo, Mun-Il
    • Korean journal of applied entomology
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    • v.51 no.3
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    • pp.215-222
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    • 2012
  • Pupation site selection behavior of Plodia interpunctella (H$\ddot{u}$bner) in response to its larval parasitoid, Bracon hebetor Say, was evaluated. Under the presence of the parasitoid, more moth larvae pupated in a layer deeper than 5 mm. Because the parasitoid searched for the host mostly in the surface layer (< 5 mm), larvae in deeper layers were able to successfully escape attack by the parasitoid. However, this behavior did not affect the numerical response of the parasitoid. The parasitoid laid more eggs per host larvae when the number of attacked larvae decreased.

Infection of Parasitic Anisakis Type Larvae (Nematoda) from Some Rockfishes, Sebastes spp. (볼락류 Sebastes spp.에 있어서 선충류 아니사키스 유충의 감염)

  • Chun, Kae-Shik
    • Journal of Food Hygiene and Safety
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    • v.17 no.4
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    • pp.206-209
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    • 2002
  • From February through March 2002, sixteen rockfishes representing three species were collected from Oregon Coast Aquarium and Newport fish market and examined far nematodes. During this study, a total of 157 Anisakis type larvae were recovered from Sebastes spp. and parasitized for an average infection prevalence of 50.0%. The yellowtail rockfish, S. flavidus, darkblotched rockfish, S. crameri, and canary rockfish, S. pinniger examined were infected with 110 Anisakis simplex larva, 30 Contracaecum spp. larva and 17 Pseudoterranova decipiens larva. Of the rockfishes examined, 75.0% carried Sebastes crameri, and 12.5% S. flavidus and S. crameri, respectively. The sites of infection far Anisakis type larvae were the intestine, omentum, and stomach walls. A. simplex larvae infected 60.0% on S. crameri and 33.3% on S.flavidus and S. pinniger respectively. The prevalence of Contracaecum sp. larvae and Pseudoterranova decipiens larvae on S. pinniger was 33.3%. The intensities of A. simplex larvae ranged from 1 to 87 in S. flavidus, S. crameri, and S. pinniger. The intensity of Contracaecum spp. larvae and P. decipiens larvae ranged from 0 to 30 in S. pinniger.

Relationship between Egg Size and Quantitative Characteristics in Silkworm, Bombyx mori (교잡원종에 있어서 잠란의 크기가 계량형질에 미치는 영향)

  • 김충곤;노시갑
    • Journal of Sericultural and Entomological Science
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    • v.34 no.2
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    • pp.13-19
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    • 1992
  • This study was investigated the relationship between egg size and manifestation of quantitative characteristics such as larval growth, cocoon weight and cocoon shell weight etc. There was a high positive correlation between egg size and body weight of newly hatched larva. Until 2nd instar, larval growth was affected by egg size. The body weight of grown larva from big eggs were heavier on all strains except for strain El6 and the larval duration from big egg were shorter on the Japanese and the Chinese skins. These facts show that the characteristics of larval body weight and larval duration were affected more by the strain than by the egg size. The cocoon characteristics of big eggs were better in comparison than those of regular eggs.

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Mosquito Larvicidal Activity of Synechocystis PCC6803 Transformed with the cry11Aa gene to Culex tritaeniorhynchus and Anopheles sinensis (Cry11Aa 유전자로 형질전환된 Synechocystis PCC6803의 작은빨간집모기와 중국얼룩날개모기 유충에 대한 살충효과)

  • 이대원
    • Korean journal of applied entomology
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    • v.43 no.1
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    • pp.35-41
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    • 2004
  • Bacillus thuringiensis produces crystal proteins toxic to medically and agriculturally important pests during sporulation. To improve the activity of insecticidal crystal protein in applying to mosquito larval control, an expression vector, pSyn4D harboring the mosquitocidal cry11Aa gene under control of psbA promoter of Amaranthus hybridus was constructed. This expression vector was transformed into Synechocystis PCC6803 and a transformant, Tr2C was selected with kanamycin. The mosquitocidal cry11Aa gene was stably integrated Into genomic DNA of Tr2C in PCR detection using cry11Aa-specific primers. The transformant expressed 72-kDa Cry11Aa protein and median lethal time (LT$\sub$50/) was approximately 2.1 days for Culex tritaeniorhynchus larvae and 0.7 day for Anopheles sinensis larvae, respectively. These results suggest this transformant can be used for mosquito larval control as a biological control agent.

Infestation of larval trematodes from fresh-water fish and brackish-water fish in River Hyungsan, Kyungpook Province, Korea (형산강 유역 담수어와 반철수어에 있어서 흡충류 피낭유충 기생상)

  • 주종윤
    • The Korean Journal of Parasitology
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    • v.22 no.1
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    • pp.78-84
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    • 1984
  • In order to determine the infestation patterns for metacercaria of digenetic trematodes, several kinds of fresh-water and brackish-water fish were caught in River Hyungsan, Kyungpook Province, Korea and examined from March to October in 1983. They were collected by netting, fishing with rod and line, or using the bait of crushed oil cake in transparent plastic bowls, and were dissected into the flesh, scales and 6ns with tail, and then examined for the presence and infestation density of larval trematodes. Of the 11 kinds of fish examined, the encysted larvae of Exorchis oviformis were found all of them, Metagonimus yokogawai from 9 kinds, Cyathocotyle orientalis, Metacercaria hasegawai and Metorchis orientalis from 6 kinds, alld Clonorchis sinensis from 4 Kinds in the present study. In the intensity of infestation with Clonorchis sinensis, the Korean shiner, Gnathopogon atromaculatus Nichols et Pope, was the most heavily infected species, the average number of metacercariae per grata of fish flesh being 45.5, followed by the striped shiner, Puntungia hirxi Ibersenstein 11.6, and Southern top-Inouthed minnow, Pseudorasbora larva (Temminck et Shlegel) , 9.7. The intensity of infestation with the iwetagonimus larvae in 8 species of fresh-water fish was relatively low, and the average number per gram of flesh varied from 0.23 to 9.1. In comparison, the brackish-water fish were more heavily infected and the mean number of cysts per gram of flesh in the sweetfish, Plecoglossus altiwelis, was 32.9. The results of this study generally indicate that Clonorchis sinensis and Metagcnimus yckogawai infection may occur by consuming raw fresh-water fish caught in the river Hyungpook, Kyungpook Province, Korea.

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Infection status with trematode metacercariae in the fresh-water fish from Chunamchosuchi (pond), Uichang-gun, Kyongsangnam-do, Korea (경남 의창군 주남저수지산 담수어의 흡충류 피낭유충 감염상)

  • 손운목;최연순
    • The Korean Journal of Parasitology
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    • v.35 no.3
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    • pp.165-170
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    • 1997
  • The present study was performed to analyze the infection status of trematode metacercariae in fishes caught from Chunamchosuchi (pond) located in Uichang-gun. Kyongsangnam-do. A total of 130 freshwater fish of 5 species was collected by a fish net and fish traps from November, 1995 to May, 1996. They were examined under a stereomicroscope after artificial digestion with pepsin-HCI solution. A total of 8 species or metacercaria, i. e. Clonorchis sinensis, Echinochnsnw japonicas, Cvathocotwle orientalis, Diplostomun sp.. Metorchis orientalis. Holostephcnw nipponicw, Exorchis oviformis and unidentified echinostome, was detected from them. The metacercariae of C. sinenesis were found in 8/20 (40.0%) Accnthorhodeus Qsmwsi, 20/20 (100%) Cutter breuiccudc, 31/45 (68.9) Cultriculus eigenmanni and 21/25 (84.0%) Pseunorasborc pronga, and the average number of metacercariae detected in each fish species were 1.9, 31.7. I5.3, and 73.0. From the above results, it was confirmed that fresh-water fishes from Chunamchosuchi (pond) were highly infected with metacercariae of avian trematode, i.e. C. orientolis, H. nipponicus. M. oui,entnlis, E. jcponicw and Diplostonum sp., and 4 species of fish. P. parvc, C. breuiccudn, C. eigenmnnni and A. asmussi, were infected with metacaecariae of C. sinensis.

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Immunohistochemical Detection of the Grolwth Hormone-like Substance in Sparganum of Spirometra erinacei (고충(Sparganum)에서 성장호르몬 유사물질의 면역조직화학적 검출)

  • 김명옥;최완성김창환
    • The Korean Journal of Zoology
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    • v.35 no.2
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    • pp.173-182
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    • 1992
  • 흰쥐에 Spirometro rrinoce측 제3기 유충(고충 sparganum)이 감염되었을때 유충에서 생성된 성장호르몬 유사물질이 흰쥐 성장에 미치는 영향을 조사하기 위하여, 감염 기일이 경과에 따라 흰쥐의 뇌하수체와 유충의 체내에서 성장호르몬 분비세포를 면역조직화학염색으로 검색하였다. 유충의 표피와 표피성 근육층 및 근질 근육층에서 면역반응성을 띄는 성장 호르몬이 동정되었으며 감염시기의 경과에 따라 성장호르몬 분비세포의 수가 점차 증가하였다. 이와는 대조적으로 흰쥐의 뇌하수체에서는 성장호르몬 분비세포의 수가 감염 기일의 경과에 따라 정상대조군에 비하여 점차 감소 하였으며, 감염후 3개월이 경과되면 다시 증가하여 정상대조군의 수준으로 회복되었다. 또한 유충에 감염된 기일의 경과에 따른 횐쥐의 혈중 성장호르몬 농도변화를 dot-ELISA 방법으로 추정한 결깍 정상대조군의 성장호르몬의 양과 유의한 차이는 없었다. 결론적으로 유충의 체내에서 생성된 성장호르몬 유사물질이 전이숙주인 흰쥐의 성장을 유도할 것으로 사료된다.

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Leaf Characteristics of Leguminous Plants and the Biology of Tobacco Cutworm, Spodoptera litura Fabricius: I. The Larbal Development and Leaf Feeding Amount (두류 품종별 잎특성과 담배거세미나방의 생태 연구: I. 유충발육과 식엽량)

  • 배순도
    • Korean journal of applied entomology
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    • v.38 no.3
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    • pp.217-224
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    • 1999
  • This study was conducted to determine the larval development of tobacco cutworm, Spdodoptera litura Fabricius, reared on leaves of different leguminous plants of 11 varieties or cultivars, and to measure amount of leaves fed by the larva. Larval duration ranged from 11.5 to 15.7 days depending on different food with the shortest on geomjeongkong-1 and the longest on daek-wangddangkong. Among 6 larval development stages, the 1st instar stages was the longest(3.2~5.0 days) while the 4th instar was the shortest (1.0~1.5 days). In general, amount of leaves consumed was increased with larval age, and consumed from 5 to 74% of total food only during the last instar stage. And female consumed more food than male. While, larval mortality and the sex-ratio seem to have no relation with the amount of food per species.

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