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Parthenogenetic Activation and Development of Freshly Matured Bovine IVM Oocytes (체외성숙 직후 소 난포란의 단위발생과 체외발육능)

  • 정희태;임석기;오세훈;박춘근;양부근;김정익
    • Korean Journal of Animal Reproduction
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    • v.21 no.1
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    • pp.71-78
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    • 1997
  • This study was conducted to investigate the activation condition of freshly matured bovine IVM oocytes for use as a cytoplasmic recipient in nuclear transfer. Bovine oocytes matured in vitro for 22-24 h were treated with various activation conditions. In Experiment 1 in vitro matured oocytes were treated with electric stimuIus (ES; 2 pulses of 1.25 kV/cm for 70 ${\mu}{\textrm{s}}$ec, each pulse 1 sec apart), ethanol (ET; 7%, 5min) , Ca$^2+$-ionophore(A23187; 10$\mu\textrm{g}$/ml, 5min) and cycloheximide(CH; 10$\mu\textrm{g}$/ml, 6 h). Activation rates were similar in treatments with ES, ET and A23187(48.8~54.3%), however, significantly reduced with CH treatment(15.9%, P

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