• Title, Summary, Keyword: 혈액조성

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hEPO 형질전환 돼지 후대의 임신기간내 혈액조성 변화

  • 이연근;이현기;이풍연;김성우;김정호;박진기;장원경
    • Proceedings of the KSAR Conference
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    • pp.38-38
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    • 2003
  • 본 연구는 사람 조혈촉진유전자(hEPO)가 도입된 형질전환돼지 Fl 수컷과 암컷간 교배로 F2 생산시, Fl 모돈에 대한 임신기간(약 114일)내 혈액조성(적혈구, 백혈구, Hemoglobin, Hematocrit value, MCV, MCH, MCHC, 혈소판) 변화를 구명하기 위하여 실시하였다. 시험에 이용된 암퇘지는 형질전환 F1 암컷 2마리와 일반돼지 1마리였고 교배와 동시에 임신기간동안(114일) 5일 간격으로 5ml의 혈액을 경정맥(jugular vein)에서 채취하였으며 혈액조성분석은 Celltac MEK 5108K(Nihon Kohden, Japan)를 이용하였는데 얻어진 결과는 다음과 같다. 임신기간중 혈액내 백혈구의 변화는 일반돼지의 경우 13.8($\pm$2.4)$\times$$10^3$/ul 로임신기간내에 일정한 변화양상을 보였으나 형질전환돼지의 경우 29.4($\pm$19.4)$\times$$10^3$와 22.5($\pm$14.1)$\times$$10^3$/ul 으로 일반돼지보다는 높게 나타났으며 임신기간내에 매우 높은 변화 양상을 나타내었다. 또한 적혈구는 일반돼지(7.2$\pm$0.7 $\times$$10^{6}$/ul)에 비하여 형질전환돼지(11.5$\pm$0.5 와 11.9$\pm$0.5 $\times$$10^{6}$ul)에서 매우 높게 나타났으며 변화양상은 일반돼지와 마찬가지로 작게 나타났다. 적혈구와 함께 Hemoglobin(Hb)과 평균혈구용적율(Hematocrit value ;, HCT) 모두 형질전환돼지가 일반돼지보다 높게 나타났으며 변화양상 또한 작게 나타남으로서 사람 조혈촉진유전자(hEPO)가 형질전환된 돼지에서는 지속적으로 발현되어 조혈촉진이 이루어지고 있으며, 조혈촉진에 의해 헤모글로빈 및 적혈구가 생성되어 혈액내 높은 혈구용적율(HCT)을 이루고 있음을 알 수 있었다. 적혈구 평균용적(Mean Corpuscular Volume ; MCV), 평균적혈구혈색소량(Mean Corpuscular Hemoglobin ; MCH), 평균적혈구혈색소농도(Mean Corpuscular Hemoglobin Concentration ; MCHC) 그리고 혈소판(Platelets) 분석결과 형질전환돼지가 일반돼지보다 약간 높은 수치를 나타냈으며 변화양상 또한 유사한 결과를 나타내었다. 이와 같은 시험분석결과를 토대로 사람 조혈촉진유전자(hEPO)가 형질전환된 돼지는 유즙으로 발현할 수 있도록 형질전환 되었음에도 불구하고 헤모글로빈 및 적혈구가 증가함으로서 형질전환돼지 개체의 혈장으로도 사람 조혈촉진인자가 분비하고 있음을 간접적으로 알 수 있었다. 정상돼지 보다 형질전환돼지가 약 30% 높은HCT 수준을 보였으며 이러한 현상은 사람에서는 적혈구증다증(erythrocytosis)으로 분류되고 있다. 이에 대한 고찰은 형질전환돼지 자체의 생리적 문제점(side effects)에 대한 해결과 더불어 기존의 인간질병에 대한 모델동물로서의 이용 가능성을 제시할 수 있는 것으로 사료된다.

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Huge Lymphangioma Developed After Arteriovenous Fistula for Hemodialysis -A case of report- (혈액투석을 위한 동정맥루 조성술후 발생한 거대림프관종 -1례 보고-)

  • 안상구;김우종
    • Journal of Chest Surgery
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    • v.29 no.9
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    • pp.1028-1030
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    • 1996
  • Arteriovenous fistula Is the most widely used mean of vascular access for long-term hemodialysis in patients with end-stage renal disease. Lymphangioma associated with arteriovenous fistula is very rare, seemed to be developed from Iymphatic fluid accumulation. Lymphangioma is benign neoplasm, arises de hobo or secondary to surge y or irradiation, and affects almost any part of the body served by the Lwphasic system. Treatment of choise for Iymphangioma Is surgical excision. We repo$\ulcorner$t a case of procedure using Gore-Tex graft between left brachial artery and cephalic vein for vascular access of hemodialysis in 59 year old female, with successful surgical removal.

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돼지 과배란 처리에 의한 배란상태와 혈액 성분과의 비교분석

  • 이연근;이현기;박진기;이풍연;이주영;정학재;장원경
    • Proceedings of the KSAR Conference
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    • pp.232-232
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    • 2004
  • 본 연구는 돼지의 발정동기화에 의한 과배란 유기시 돼지 난소의 배란상태(황체수)와 혈액내 혈액성분(WBC, RBC Hb, HCT, MCV, MCH, MCHC, Platelet)과의 비교분석을 통하여 다배란에 따른 표지(Marker) 혈액성분을 구명하기 위하여 실시하였다. 돼지 난소의 배란상태를 구명하기 위하여 30두의 성축 암컷을 이용하였으며, 발정 동기화는 PG 600(PMSG 400 IU, HCG 200IU)을 주사한 후 10일 후에 Regumate porcine을 6일간 급여한 후에 PMSG 주사하고 2일 후에 hCG주사한 다음날 자연종부를 실시하여 수정에 이르게 하였다. (중략)

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Effect of Feeding the High Levels of Microcapsulated Inulin on Egg and Blood Lipid Profile in Laying Hens (산란계의 혈액 및 계란지질에 관한 고수준의 미세캡슐화 이눌린 급여효과)

  • Park, Sang-Oh;Park, Byung-Sung
    • Journal of the Korean Applied Science and Technology
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    • v.29 no.2
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    • pp.214-223
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    • 2012
  • This study was carried out to investigate the feeding effects of microcapsulated inulin (MI) from Jerusalem artichoke (Helianthus tuberosus L.) on blood lipid, fatty acid composition and cholesterol of egg in laying hens. Hyline brown layers of 25 weeks old were subjected to one of the following treatments for 4 weeks: a control group without MI, 800 ppm, 900 ppm, and 1,000 ppm. Compared with control group, levels of triacylglyceride and cholesterol in blood and egg cholesterol decreased significantly in groups MI groups. Egg saturated fatty acid was lower in MI groups than control group but not unsaturated fatty acid was significantly high in MI groups as compared to the group without MI. The results suggest that the addition of microcapsulated inulin to a laying hens' diet can improve egg quality by reducing blood lipids.

Effects of Dietary Supplementation of Mulberry Leaves and Dandelion Extracts on Performance and Blood Characteristics of Chickens (뽕잎과 민들레 추출물 급여가 육계의 생산성 및 혈액성상에 미치는 영향)

  • Park, Chang-Ill;Shon, Joong-Cheon;Kim, Young-Jik
    • Korean Journal of Poultry Science
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    • v.37 no.2
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    • pp.173-180
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    • 2010
  • This study was conducted to investigate the effects of dietary supplementation of mulberry leaves and dandelion extracts on performance, proximate composition, heating loss, drip loss, and blood characteristics of chickens. One hundred sixty broiler chicks were fed diets for five weeks containing 1% mulberry leaves extracts (T1), 2% mulberry leaves extracts (T2), 1% dandelion extracts (T3), and 2% dandelion extracts (T4). At the end of five week feeding experiment, broiler were slaughtered, and stored at $4^{\circ}C$. T2 resulted in much better weight gain and feed conversion than other treatment groups. As storage time increased, all treatment groups resulted in increased heating loss and drip loss (P<0.05) but no significant difference were observed among the treatment groups. In blood composition, GOT (glutamic oxaloacetate transaminase), GPT (glutamic pyrubic transaminase), total cholesterol, and triglyceride were significantly decreased by the supplementation of mulberry leaves and dandelion extracts compared to the control (P<0.05). Especially, T4 was significantly (P<0.05) more effective in improving blood composition compared to other treatment groups. However, no significant difference (P>0.05) were found in LDL-cholesterol among all treatment groups. In conclusion, these data indicate that compared to other treatments, supplementation of 2% dandelion extracts (T4) were most effective in decreasing total cholesterol and triglyceride and increasing HDL-cholesterol.

Giant Venous Aneurysm after Brachiocephalic Arteriovenous Shunt for Hemodyalisis Access -A case report- (혈액투석을 위한 상완동맥-두정맥단락 수술 후 발생한 거대한 정맥의 동맥류 -1예 보고-)

  • Chon, Soon-Ho;Lee, Chul-Burm
    • Journal of Chest Surgery
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    • v.39 no.11
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    • pp.861-863
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    • 2006
  • We report a case of a 46-year-old man with end-stage renal failure who developed a giant aneurysm after a brachiocephalic arteriovenous shunt. The patient had complaints of pulsating pain and swelling of his left upper extremity The patient had abandoned use of the arteriovenous shunt and had a second arteriovenous shunt procedure over his right extremity. The giant venous aneurysm was removed just distal to his anastomosis. The patient's postoperative course was uneventful.

The Relationship between Cell-mediated Immunity and Subtypes of Lymphocyte in BAL Fluid and Peripheral Blood in Patients with Pulmonary Tuberculosis (폐결핵 환자의 말초 혈액 및 기관지 폐포세척액내의 임파구 아형과 세포성 매개면역과의 관계)

  • Uh, Soo-Taek;Cha, Mi-Kyung;Lee, Sang-Moo;Kim, Hyun-Tae;Chung, Yeon-Tae;Woo, Jun-Hee;Kim, Yong-Hun;Park, Choon-Sik
    • Tuberculosis and Respiratory Diseases
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    • v.39 no.4
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    • pp.334-342
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    • 1992
  • Background: The activated T lymphocyte by inhalaed mycobacterial antigen may evoke cell-mediated immunity in patients with active pulmonary tuberculosis. These activated lymphocyte may influence the response of tuberculin-purified protein derivative (PPD) in skin test. But occasionally, anergy to PPD appear in patients with pulmonary tuberculosis in spite of active stage. Thus we evaluated the effect of change of subtypes of lymphocyte in bronchoalveolar lavage fluid (BAL) and peripheral blood on anergy to PPD in patients with active pulmonary tuberculosis. Method: We performed tuberculin skin test and flow-cytometry analysis of lymphocytes obtained from BAL fluid and peripheral blood in 11 healthy normal volunteers and 20 patients with active pulmonary tuberculosis. Results: 1) The composition of lymphocyte significantly increased in patients with active pulmonary tuberculosis when compared with that in healthy control ($25.2{\pm}4.8$ vs $6.5{\pm}1.3%$, p<0.01), but composition of monocyte significantly decreased ($69.6{\pm}5.7$ vs $89.2{\pm}1.4%$, p<0.05) in analysis of BAL fluid. 2) There were no differences in compositions of cells in BAL fluid between responders and no-responders to PPD. 3) The compositions of CD3 (+), CD4 (+), CD3 (+) IL-2R (+), CD3 (+) HLA-DR (+) significantly increased in BAL fluid when compared with those in peripheral blood in patients with active pulmonary tuberculosis. But the composition of CDS (+), CD4/CDS were not different between BAL fluid and peripheral blood. 4) There were no correlations between response to PPD and compositions of cells and lymphocyte subtypes in BAL fluid and peripheral blood in all patients with tuberculosis, responders, and no-responders, respectively. Conclusion: From these results, we suggest no direct relationship between compositions of inflammatory cells in bronchoalveolar lavage fluid and we could not rule out the possibility of compartmentalization of activated lymphocyte involving in anergy to PPD in skin test in patients with active pulmonary tuberculosis.

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