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Validation of Prediction Equations of Energy Values of a Single Ingredient or Their Combinations in Male Broilers

  • Alvarenga, R.R.;Rodrigues, P.B.;Zangeronimo, M.G.;Oliveira, E.C.;Mariano, F.C.M.Q.;Lima, E.M.C.;Garcia, A.A.P. Jr;Naves, L.P.;Nardelli, N.B.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.28 no.9
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    • pp.1335-1344
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    • 2015
  • A set of prediction equations to estimate the nitrogen-corrected apparent metabolizable energy (AMEn) of individual ingredients and diets used in the poultry feed industry was evaluated. The AMEn values of three energy ingredients (maize, sorghum and defatted maize germ meal), four protein ingredients (soybean meal, maize gluten meal 60% crude protein, integral micronized soy and roasted whole soybean) and four diets (three containing four feedstuffs, complex diets, and one containing only corn-soybean meal, basal diet) were determined using a metabolism assay with male broilers from 1 to 7, 8 to 21, 22 to 35, and 36 to 42 days old. These values were compared to the AMEn values presented in the tables of energy composition or estimated by equation predictions based on chemical composition data of feedstuffs. In general, the equation predictions more precisely estimated the AMEn of feedstuffs when compared to the tables of energy composition. The equation AMEn (dry matter [DM] basis) = 4,164.187+51.006 ether extract (% in DM basis)-197.663 ash-35.689 crude fiber (% in DM basis)-20.593 neutral detergent fiber (% in DM basis) ($R^2=0.75$) was the most applicable for the prediction of the energy values of feedstuffs and diets used in the poultry feed industry.

Planning Large Program of Stellar Maser Study with KaVA

  • Cho, Se-Hyung;Imai, Hiroshi
    • The Bulletin of The Korean Astronomical Society
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    • v.39 no.2
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    • pp.114-114
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    • 2014
  • We present our activities linking to planning of possible forms of large program to study on circumstellar H2O and SiO maser sources with KaVA. A great advantage of KaVA for the stellar maser observations is the combination of the unique capability of the multi-frequency phase referencing technique of KVN and the dual-beam astrometry of VERA with the KaVA's relative dense antenna configuration. We have demonstrated this advantage through the test observations conducted by the KaVA Evolved Stars Sub-working Group since 2012 March. Snapshot KaVA imaging is confirmed to be possible in integration time of 0.5 hour at the 22 GHz band and 1.0 hour at the 43 GHz band in typical cases. This implies that large snapshot imaging surveys towards many H2O and SiO stellar masers are possible within a reasonable machine time (e.g., scans on ~100 maser sources within 200 hours). This possibility enables us to select the maser sources, which are suitable for future long-term (10 years) intensive (biweekly-monthly) monitoring observations, from 1000 potential target candidates selected from dual-frequency band (K/Q-bands) KVN single-dish observations. The output of the survey programs will be used for statistical analysis of the structures of individual stellar maser clumps and the spatio-kinematical structures of circumstellar envelopes with accelerating outflows. The combination of astrometry in milliarcsecond(mas) level and the multi-phase referencing technique yields not only trigonometric parallax distances to the masers but also precise position reference for registration of different maser lines. The accuracy of the map registration affects interpretation of the excitation mechanism of the SiO maser lines and the origin of the variety of the maser actions, which are expected to reflect periodic behaviors of the circumstellar envelope with stellar pulsation. Currently we are checking the technical feasibility of KaVA operations for this combination. After this feasibility test, the long-term monitoring campaign program will run as one of KaVA's legacy projects.

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Development and Application of Classroom Homepage Using Wiki (지식공유기법을 활용한 학급 홈페이지의 개발 및 적용)

  • Kim, Yu-Song;Yoo, In-Hwan
    • Journal of The Korean Association of Information Education
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    • v.10 no.1
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    • pp.13-22
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    • 2006
  • Students should be able to find information and resources using the Internet. Also students need to have composite intellectual abilities to select information, convert to knowledge and communicate it to other people. However, most school and classroom homepages provide students with information but not contents that help them produce knowledge. This lowers the rate of connection to homepage. Thus, the purpose of this study is to develop and apply homepage, which helps students make knowledge from comprehended and interpreted information(Knowledge.Sharing Technique; KST). In this study we use 'WIKI KST' and 'Q&A KST' to share knowledge. WIKI KST is 'an encyclopedia that we make,' which means that students upload their prior knowledge or knowledge produced from comprehended and interpreted information and create new knowledge to be added to their own knowledge and others'. As a result, the abilities to comprehend and interpret acquired information are improved and the ability to change implicit knowledge to explicit knowledge is also improved. Students create new knowledge to share their knowledge and, as a result, the rate to connection to homepage gets higher.

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Relationship Between Body Image and Self-esteem in Patients with Chronic Renal Failure (만성신부전증 환자의 신체상과 자아존중감의 관계)

  • Sohn Sue-Kyung;Lee Myung-Hwa;Song Myung-Sook
    • Journal of Korean Academy of Fundamentals of Nursing
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    • v.8 no.2
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    • pp.160-171
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    • 2001
  • Purpose: The purpose of study was to identify the relationship between body image and self-esteem in patients with chronic renal failure. Method The subjects of study were 97 patients with chronic renal failure who were outpatients at K medical center. B hospital, H hospital in Busan. Data were collected from Nov 15th to Dec. 17th, 1999. The instruments used for study were the Body Cathexis Scale and Rogenberg's Self-esteem Scale. Collected data were analyzed by frequency, percentage, mean, standard deviation, t-test, ANOVA, Scheffe test, and Pearson's correlation coefficients. Result: 1 The mean score for body image was $137.46{\pm}22.21$, the mean mark $2.92{\pm}0.47$, and the mean score of self-esteem was $30.80{\pm}8.76$, the mean mark $3.08{\pm}0.87$. 2. There was a significant difference in the score for body image according to educational level (F=5.189, P=.002) and type of residence (F=6.095. P=.001). 3. There was a significant difference in the score for self-esteem according to age (F=3.615, P=.009), educational level (F= 4.772, P=.004), marital status (F=3.498 P=.019) and presence of children (F=2.511, P=.014). 4. Body image in patients with chronic renal failure patients showed significant correlation with self-esteem (r=.519, P=.000). Conclusion: From this study, a relationship between body image and self-esteem in patients with chronic renal failure was identified. Therefore, nursing interventions are needed to promote body image and self esteem in patients with chronic renal patients.

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Cloning, Expression, and Characterization of a Hyperalkaline Phosphatase from the Thermophilic Bacterium Thermus sp. T351

  • Choi Jeong-Jin;Park Jong-Woo;Shim Hye-Kyung;Lee Suk-Chan;Kwon Moo-Sik;Yang Joo-Sung;Hwang Heon;Kwon Suk-Tae
    • Journal of Microbiology and Biotechnology
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    • v.16 no.2
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    • pp.272-279
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    • 2006
  • The gene encoding Thermus sp. T351 alkaline phosphatase (T351 APase) was cloned and sequenced. The gene consisted of 1,503 bp coding for a protein with 500 amino acid residues including a signal peptide. The deduced amino acid sequence of T351 APase showed relatively low similarity to other Thermus APases. The T351 APase gene was expressed under the control of the T7lac promoter on the expression vector pET-22b(+) in Escherichia coli BL21 (DE3). The expressed enzyme was purified by heat treatment, and $UNO^{TM}$ Q and $HiTrap^{TM}$ Heparin HP column chromatographies. The purified enzyme exhibited high activity at extremely alkaline pHs, reaching a maximum at pH 12.0. The optimum temperature of the enzyme was $80^{\circ}C$, and the half-life at $85^{\circ}C$ was approximately 103 min. The enzyme activity was found to be dependent on metal ions: the addition of $Mg^{2+}$ and $CO^{2+}$ increased the activity, whereas EDTA inhibited it. With p-nitrophenyl phosphate as the substrate, T351 APase had a Michaelis constant ($K_{m}$) of $3.9{\times}10^{-5}M$. The enzyme catalyzed the hydrolysis of a wide variety of phosphorylated compounds.

Plant Community Survey and Analysis for Restoration of Vegetation in Coal-mined Spoil Lands - A Case Study of Hamtae Coal-mined Spoil Lands in Taebaek City, Kangwondo - (폐탄광 경석지 식생 복원을 위한 식물군집 조사·분석 - 강원도 태백시 함태탄광 폐탄광지를 사례로 -)

  • Kim, Bo-Hyun;Kim, Kyung-Hoon;Kim, Hyea-Ju;Kim, Doo-Ha
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.3 no.4
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    • pp.33-42
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    • 2000
  • This study is carried out to investigate plant community structure, actual vegetation map and flora for examining the relationship between naturally introduced vegetation and surrounding vegetation in Hamtae coal-mined spoil lands. According to the analysis of vegetation, surrounding vegetation was divided into five plant community groups : Pinus koraiensis community, Pinus densiflora - Quercus mongolica community, Q. mongolica - Deciduous broadleaved trees community, Betula schmidtii - B. davurica community, Larix leptolepis community. Robinia pseudo-acacia, which had been planted, was dominant species in coal-mined spoil lands. Young trees, which were naturally introduced, such as B. schmidtii, B. davurica etc., partially appeared with the high density($15{\sim}19ea/100m^2$) in coal-mined spoil lands. The flora of this area was listed as 54 taxa; 22 families, 42 genus, 50 species, 3 varieties and 1 forma. Especially Spodiopogon sibiricus, Miscanthus sinensis, Arundinella hirta, Artemisia capillaris, Artemisia iwayomogi and Youngia denticulata appeared with the high frequency. It is estimated that the proper way for harmonious and effective revegetation is to target B. schmidtii - B. davurica communities and to seed B. schmidtii - B. davurica mixing highly frequent herbaceous species.

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Monoclonal Antibody to CD9 Inhibits Platelet-induced Human Endothelial Cell Proliferation

  • Ko, Eun-Mi;Lee, In Yong;Cheon, In Su;Kim, Jinkoo;Choi, Jin-Suk;Hwang, Jong Yun;Cho, Jun Sik;Lee, Dong Heon;Kang, Dongmin;Kim, Sang-Hyun;Choe, Jongseon
    • Molecules and Cells
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    • v.22 no.1
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    • pp.70-77
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    • 2006
  • Platelets are anucleate cytoplasmic fragments derived from bone marrow megakaryocytes, and endothelial cells constitute the barrier between bloodstream and adjacent tissues. Although platelets are thought to regulate the biological functions of endothelial cells, the molecular mechanisms involved are poorly understood. With human umbilical vein endothelial cells and freshly isolated platelets, we established an in vitro model of platelet-induced endothelial cell proliferation. Platelets stimulated endothelial cell proliferation in a dose-dependent manner and transwell experiments with semi-permeable membranes suggested that direct cell-to-cell contacts were required. We developed mAbs against platelets and selected a mAb that blocks their proliferative effect. We purified the antigen by immunoprecipitation and identified it by Q-TOF MS analysis as the tetraspanin CD9. Since both platelets and endothelial cells expressed CD9 strongly on their surfaces we carried out a pre-treatment experiment that showed that CD9 molecules on the endothelial cells participate in the mitogenic effect of the platelets. The inhibitory effect of our mAb was comparable to that of a well-known functional anti-CD9 mAb. These results suggest that the tetraspanin CD9 plays an important role in endothelial regeneration.

A Study on Menstrual Symptoms and Pattern according to the Sasang Constitution in High School Girls in Pusan (여고생의 사상체질(四象體質)에 따른 월경양상에 관한 연구)

  • Park, Hae-Woong;Kim, Dong-Il
    • The Journal of Korean Obstetrics and Gynecology
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    • v.20 no.1
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    • pp.169-185
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    • 2007
  • Purpose : The purpose of this study was to investigate menstrual symptoms and patterns of high school girls by Sasang constitution and relationship between such symptoms and Sasang constitution. Methods : For the study, QSCCII and 'M.B.Q' employed to analyze the data collected from 581 high school girls in Pusan from July 11 to July 20, 2006. Results : As for menstrual characteristics of the subjects by Sasang constitution Taeeumins start menarche is relatively early in contrast with Soeumins who experienced menarche relatively late(P=.012). This survey proves that menses last relatively long for Soeumin whereas Soyangins have relatively short period of menstruation(P=.043). Furthermore, Taeeumins account for majority of those who have much quantity of menstruation with 22.7% while Soeumins account for majority with 13.2%. From the analysis into menstrual symptoms of the subjects put under 5 categories of Sasang constitution, it became evident that Soeumins turned out to have most negative affect(11.8${\pm}$3.78) (P=.053). Soeumins scored the highest points in the response area of autonomic reactions(10.6${\pm}$3.24)(P=.014). In the total points of menstrual symptoms including the above 5 sub-categories, Soeumins recorded the highest scores(52.4${\pm}$12.5), followed by Soyangins(50.0${\pm}$12.2) and Taeeumins in order, showing that there is no statistically significant difference(F = 2.859, p = .058) among them although Soeumins seem to feel slightly more menstrual symptoms than Soyangins and Taeeumins. Conclusion : We hope these results could be helpful to diagnosis and treatment of mestrual diseases.

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Ecological Environment of Native Habitats and Host Plant in Mistletoe (Viscum album var. coloratum) (겨우살이의 서식지생태환경과 기주식물)

  • Lee, Bo-Duk
    • Korean Journal of Plant Resources
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    • v.22 no.5
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    • pp.389-393
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    • 2009
  • Regional distribution of mistletoe (Viscum album var.coloratum) and its habitual environments were investigated in order to obtain the basal data on the artificial propagation to cope with its increasing consume for medicine. Mistletoes inhabited throughout the overall region of the South Korea investigated. They were parasitic mainly to the Quercus spp. including Q. serrata and rarely to the Castanea crenata var. dulcis, Prunus serrulata var. spontanea, Alnus japonica, and Pyrus pyrifolia, etc. Mistletoes were not observed on the conifers such as Pinus densiflora and Pinus koraiensis and some deciduous broad-leaved trees species such as Zelkova serrata, Diospyros kaki, Acer mono, Acer palmatum, and Morus alba. Their habitats were located from zero to 1,200 m above sea level nevertheless the direction or slope of the mountains, suggesting that artificial propagation can be carried out nation widely to the well-grown parasite tree species. Parasitic specificity related to the physical and chemical characteristic of the epidermal tissues will be studied further.

Immobilization of Keratinolytic Metalloprotease from Chryseobacterium sp. Strain kr6 on Glutaraldehyde-Activated Chitosan

  • Silveira, Silvana T.;Gemelli, Sabrine;Segalin, Jeferson;Brandelli, Adriano
    • Journal of Microbiology and Biotechnology
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    • v.22 no.6
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    • pp.818-825
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    • 2012
  • Keratinases are exciting keratin-degrading enzymes; however, there have been relatively few studies on their immobilization. A keratinolytic protease from Chryseobacterium sp. kr6 was purified and its partial sequence determined using mass spectrometry. No significant homology to other microbial peptides in the NCBI database was observed. Certain parameters for immobilization of the purified keratinase on chitosan beads were investigated. The production of the chitosan beads was optimized using factorial design and surface response techniques. The optimum chitosan bead production for protease immobilization was a 20 g/l chitosan solution in acetic acid [1.5% (v/v)], glutaraldehyde ranging from 34 g to 56 g/l, and an activation time between 6 and 10 h. Under these conditions, above 80% of the enzyme was immobilized on the support. The behavior of the keratinase loading on the chitosan beads surface was well described using the Langmuir model. The maximum capacity of the support ($q_m$) and dissociation constant ($K_d$) were estimated as 58.8 U/g and 0.245 U/ml, respectively. The thermal stability of the immobilized enzyme was also improved around 2-fold, when compared with that of the free enzyme, after 30 min at $65^{\circ}C$. In addition, the activity of the immobilized enzyme remained at 63.4% after it was reused five times. Thus, the immobilized enzyme exhibited an improved thermal stability and remained active after several uses.