• Title/Summary/Keyword: ANP

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Autoradiographic Localization of Atdal Natriuretic Peptide Binding Sites in the Pig Ovary (돼지 난소 Atrial Natriuretic Peptide 결합 부위의 자가방사법에 의한 검증)

  • 김성주;김선희
    • The Korean Journal of Zoology
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    • v.38 no.4
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    • pp.523-530
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    • 1995
  • Specific affinity binding sites for atrial natriuretic peptide (ANP) were Investigated in the pig ovarian tissues by in vitro autoradiographic techniques. In the pig ovary, the highest binding sites for 12514abeiled rANP(l~28) were localized in the granulosa cell layer of the forncles. The binding sies on theca layer of the ovarian follicles were mainly localized in the external layer, but none was observed In the Internal layer. In the corpus luteum, the binding site was not observed. The specific bindings of 200 pM of l2Sl4abelled rANP(l~28) to granulosa and theca externa layers were reversed completely by excess concentration (1 ~4) of unlabelled rANP(l~28) but not by 10 ~ of unrelated peptides, human angiotensin II and arginine vasopressin. The binding was also displaced by 1 ~ of desiGIn18, Ser19, Gly2O, leu21, Gly22I ANP(4~2g) (C- ANF) as a spedfic ligand of the ANP clearance receptor. Therefore these results indicate ~hat the biological and the clearance ANP receptors exist in the theca externa and granulosa layer of the pig ovary, and suggest that the ANP receptors may be related with the regulatory lundion of the ovarian follicular development including oocyte maturation.

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Characteristics of hypoxia-induced ANP Secretion in Perfused Beating Atria (허혈성 자극에 의한 심방이뇨 호르몬 분비 반응의 특성)

  • Kim, Kong-Soo;Kim, Min-Ho;Kim, Chang-Gon;Kim, Suk-Kee;Cho, Kyung-Woo;Cui, Xun
    • Journal of Chest Surgery
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    • v.33 no.5
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    • pp.398-406
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    • 2000
  • Background: Cardiac atrium is an endocrine gland secreting a family of natriuretic peptides. The secretion of atrial natriuretic peptide(ANP) had been shown to be controlled by variable factors. The change in atrial dynamics have been considered as one of the most prominent stimuli for the stimulation of ANP secretion. Hypoxic stress has been shown to increase cardiac ANP secretion. However, the mechanism by which hypoxia increases ANP secretion cardiac ANP secretions. However, the mechanism by which hypoxia increases ANP secretion has not to be defined. Therefore, the purpose of the present study was tow-fold: to develop a protocol to defined the effect of hypoxia on ANP secretion in perfused beating rabbit atria and to clarify the mechanism responsible for the accentuation by hypoxia of ANP secretion. Material and Method: Experiments have been done in perfused beating rabbit atria. ANP was measured by radioimmunoassay. Result: Hypoxic stimulus with nitrogen decreased atrial stroke volume. The decrease in atrial stroke volume recovered basal level during the period of recovery with oxygen. ANP secretion and the concentration of perfusate ANP in terms of extracellular fluid(ECF) translocation which reflects the rate of myocytic release of ANP were increased by hypoxia and returned to basal levels during the recovery. Changes in ECF translocation paralleled by hypoxia and returned to basal levels during the recovery. Changes in ECF translocation paralleled to that of atrial stroke volume. At the start of recovery in atrial storke volume, ECF tranalocation incrased for several minutes. The above responses were stable and reproducible. Glibenclamide treatment prevented the recovery in atrial stroke volume. Increments by hypoxia of ANP secretion and ANP concentration were suppressed by glibenclamide. Conclusion: These results indicate that hypoxia incrased atrial myocytic ANP release and that the mechanism responsible for the accentuation is partially related to the change in K+ATP channel activity.

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Effects of activation of protein kinase C on the regulation of atrial natriuretic peptide(ANP) by isolated perfused left atria (백서의 심방관류모델에서 protein kinase C의 활성화가 atrial natriuretic peptide(ANP) 조절에 미치는 영향)

  • Kang, Chang-won;Kang, Hyung-sub;Lee, Ho-il
    • Korean Journal of Veterinary Research
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    • v.37 no.4
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    • pp.735-744
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    • 1997
  • 심방근 세포는 심방이뇨호르몬을 합성, 저장 그리고 분비하며, 세포내외 이온의 농도, 수분균형 및 혈압 등을 조절하는 것으로 알려져 있다. 또한 심방근의 인장자극에는 Atrial Natriuretic Peptide(ANP)를 2단계(분비, 유리)의 과정으로 이루어져 있으며, 이에 따른 심방이뇨호르몬의 분비 조절기전에 대하여서는 명확히 알려져 있지 않다. 따라서 본 연구는 백서의 심방근 적출관류 모델을 이용하여 protein kinase C와 ANP 조절의 상관관계를 밝히고 분비와 유리의 과정중 어떠한 과정을 이용하여 분비자극에 영향을 주는지를 관찰하기 위하여 본 실험을 실시하였다. PKC 활성제인 PMA(phorbol 12-mystrate 13-acetate)는 ANP의 유리를 현저하게 증가시켰으며, PKC 억제제인 H-7(1-(5-isoquinoline sulfonyl)-2-methyl piperazine dihydrochlo-ride)에 의해 유리를 억제시켰다. PMA와 H-7을 동시에 처리한 경우 PMA에 의하여 증가된 ANP의 유리가 H-7에 의하여 차단됨을 관찰할 수 있었다. 따라서 백서의 관류 심방에서의 ANP 분비 증가는 PKC 활성화에 의하여 이루어지며, ANP분비의 2단계중 ANP 유리에 영향을 줌을 알 수 있었다.

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Postnatal Changes in Atrial Compliance and Stretch-Induced ANP Secretion in Rabbits

  • Kim, Suhn-Hee;Lee, Kyung-Sun;Kim, Sung-Zoo;Seul, Kyung-Hwan;Cho, Kyung-Woo
    • The Korean Journal of Physiology and Pharmacology
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    • v.4 no.5
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    • pp.393-401
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    • 2000
  • To define the postnatal changes in ANP secretion in response to mechanical stretch and atrial compliance, experiments have been done in perfused nonbeating rabbit atria with different ages: 1-day, 1-, 2-, 3-, 4-, and 8-wk-old. In 1-day-old-rabbits, an increase in intraatrial pressure resulted in an increase in atrial volume, which was higher than that in 1-wk-old rabbits. Increases in atrial volume stimulated the secretion of ANP with concomitant translocation of extracellular fluid (ECF) into the atrial lumen. However, mechanically stimulated ECF translocation was lower in 1-day-old rabbits than that in 1-wk-old rabbits. Therefore, positive relationship between mechanically stimulated ECF translocation and ANP secretion was shifted upward in 1-day-old rabbits, as compared to 1-wk-old rabbits. Changes in atrial volume and ECF translocation were gradually increased with aging and reached the peak value at 4 wk. The stretch-induced ANP secretion in terms of ECF translocation (the interstitial ANP concentration) was also increased with aging and reached the peak value at 4 wk. The interstitial ANP concentration was dependent on the atrial content of ANP. These data suggest that the higher level of atrial ANP secretion is related to the postnatal changes in atrial volume and unidentified factor.

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Effects of Intrarenal Arterial Infusion of Pro-Atrial Natriuretic Peptides on Renal Function in Unanesthetized Rabbits (가토 신장기능에 미치는 Pro-Atrial Natriuretic Peptide의 영향)

  • Lee, Jeong-Eun;Cho, Kyung-Woo;Kim, Suhn-Hee
    • The Korean Journal of Physiology
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    • v.24 no.1
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    • pp.131-144
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    • 1990
  • It is well known that the atrial natriuretic peptide (ANP) has a prepro-hormone of 151 amino-acids which loses their hydrophobic signal peptide to form 126 amino acid prohormone. The whole prohormone is released and then cleaved by proteases into more than one circulating forms. Recently, Winters et al. (1988a, b) reported that high concentrations of N-terminal fragments of prepro-ANP $(26{\sim}55),\;(56{\sim}92)\;and\;(104{\sim}123)$ were detected in human plasma. However, their physiological roles have not been established. The present study was conducted to determine whether the N-terminal fragments of pro-ANP have any effect on the renal function and to compare the effect with those of G-terminal fragments of pro-ANP The results indicate that intrarenal arterial infusions of prepro-ANP $(26{\sim}41),\;(26{\sim}55),\;(56{\sim}92)\;and\;(104{\sim}123)$ induced no significant changes in renal function. Whereas ${\alpha}-human$ ANP $(prepro-ANP,\;124{\sim}151)$ and pro-ANP caused a significant increase in urine volume, renal plasma flow, glomerular filtration rate, urinary excretions of sodium, chloride and potassium, and fractional excretion of sodium. These results suggest that the N-terminal fragments of pro-ANP are ineffective, while the C-terminal fragments retain the natriuretic and diuretic activities.

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Fuzzy ANP Application for Vender Prioritization (공급업체 우선순위 선정을 위한 Fuzzy ANP의 활용)

  • Jung, Uk
    • Journal of the Society of Korea Industrial and Systems Engineering
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    • v.34 no.2
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    • pp.9-18
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    • 2011
  • Vender prioritization process is one of the most critical tasks of production and logistics management for many companies. Determining the most critical criteria for vender prioritization process is a vital means for a purchasing company to improve its supply chain productivity. This study discuss the use of a Fuzzy analytic network process (Fuzzy ANP) model which is an efficient tool to handle the fuzziness of the data involved in deciding the preferences of different criteria which are not independent. Also, the comparison of classical ANP and Fuzzy ANP is described using simulation with triangular distribution random number generation. It is shown that Fuzzy ANP model possesses some attractive properties and could be used as an alternative to the known vender prioritization methods.

A study on the degree of influence of technology by AHP and ANP (AHP와 ANP를 이용한 기술기여도에 관한 연구)

  • Hong, Du-Wha;Chung, Min-Yong
    • Journal of the Korea Safety Management & Science
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    • v.8 no.4
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    • pp.167-180
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    • 2006
  • The ANP(Analytic Network Process), though based on the AHP(Analytic Hierarchy Process), is a system for the analysis, synthesis, and justification of complex decisions with the capability to model non-linear relations between the elements. ANP allows the decision makers to leap beyond the traditional hierarchy to the interdependent environment of network modeling. The ANP is designed for problems characterized by the added complexity of interdependencies such as feedback and dependencies among problem elements. Using a network approach makes it possible to represent and analyze interactions, incorporate non-linear relations between the elements, and synthesize mutual effects by a single logical procedure. This study intends to evaluate the contribution of technology in intangible assets by the AHP and ANP.

Prokinetic Activity of Ethanolic Extracts from Dried Citrus unshiu Peels in Mice (귤나무 과피 유래 한약재 주정 추출물의 위장관 운동 촉진 효과)

  • Lee, Hyun-Tai
    • Journal of Life Science
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    • v.24 no.3
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    • pp.260-265
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    • 2014
  • Dried Citrus unshiu peels (Aurantii Nobilis Pericarpium; ANP) are used as a traditional folk medicine for the treatment of gastrointestinal (GI) motility disorders in East Asia, including Korea. In the present study, an ethanolic extract of ANP (ANP-E) exhibited no significant toxicity in mice, even at an oral dose of 5 g/kg. The effects of ANP-E on GI motor function were investigated by measuring the intestinal transit rate (ITR) of Evans blue in normal mice and mice with experimental GI motility dysfunction (i.e., peritoneal irritation by acetic acid; PIA). In normal mice, ANP-E significantly increased the ITR in a dose-dependent manner. The ITR in the PIA mice was significantly retarded compared to that in the normal mice. However, ANP-E significantly inhibited this retardation in a dose-dependent manner. Furthermore, in all the models, the potency of ANP-E appeared to be same or higher than that of cisapride, which was used predominantly for the treatment of various GI motility disorders in humans in the 1900s but was removed from the market in 2000 due to fatal side effects. The results suggest that ANP-E has potential as a new prokinetic agent that could be used as a substitute for cisapride.

Changes of the Plasma Atrial Natriuretic Peptide during Myocardial Infarction in Rats (흰쥐 심근경색 모델에서 혈장 Atrial Natriuretic Peptide의 변화)

  • Ahn, Dong-Choon;Kim, In-Shik
    • Journal of Veterinary Clinics
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    • v.29 no.2
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    • pp.148-153
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    • 2012
  • Atrial natriuretic peptide (ANP) is associated with the variety of disorders of myocardial function. The effect, however, of myocardial infarction (MI) on ANP has not been fully described. Thus, this study investigated the effect of experimental MI, induced by left coronary artery ligation, on ANP secretion. Male Sprague-Dawley rats aged 60 d underwent ligation of the left anterior descending coronary artery to induce MI and were compared with a group that underwent a sham operation. Rats of sham operation had a similar procedure without having the suture tightened around the coronary artery. Animals were sacrificed at 1, 3, 6, 12, and 18 h or 1, 3, 5, 7, 14, and 30 d after the procedure. MI size was assessed by planimetry and perimetry, and plasma ANP levels were determined by radioimmunoassay. Mean infarct size was 39.6-44.5% of the left ventricle after coronary occlusion in experimental groups. No significant differences were observed in infarct size among groups. In contrast, the concentration of plasma ANP was significantly higher at 1, 3, 6, 12, 18, and 24 h after left coronary artery ligation than in sham animals. This parameter, however, did not differ significantly at 3, 5, 7, 14, and 30 d after ligation compared with sham-ligated controls. These results demonstrate that plasma ANP levels are markedly increased in the early phase of MI in the male rat and can be a useful biomarker for diagnosis in acute MI.

Extracorporeal Circulation Influence on Plasma Atrial Natriuretic Peptide (승모판막질환자의 체외순환술에 따른 혈중 atrial natriuretic peptide의 변화)

  • 이형민;이동협;이정철;한승세
    • Journal of Chest Surgery
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    • v.26 no.2
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    • pp.102-107
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    • 1993
  • Human atria play an important role in extracellular homeostasis through release of atrial natriuretic peptide. To evaluate the relationship between plasma level of atrial natriuretic peptide (ANP) and many changes which can develop during extracorporeal circulation, we studied 16 patients undergoing, 12 cardiac operation and 4 thoracic operation. Plasma level of ANP in cardiac patients group was significantly higher and more changeable than thoracic patients group. After aortic cross clamp release, blood was filled at right atrium and right atrial pressure was rapidly increased. At the same time, plasma level of ANP was rised suddenly. Increase of ANP level was correlated (p<0.05) with the increase of total bypass time, but was not correlated statistical with aortic cross clamp time. ANP level did not fall rapidly after aortic cross clamp while both atria were completely empty. This result was explained by intraoperative hypothermia at that time, which can inactivate plasmal endopeptidase and catalytic receptors of ANP. The ANP level of atrial fibrillation group in cardiac patients were generally higher than normal sinus group, but there was no statistical correlation.

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