• Proceedings of the Korean Society of Precision Engineering Conference
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• 2009.10a
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• pp.393-394
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• 2009

• KSBB Journal
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• v.24 no.3
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• pp.279-286
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• 2009

Streptomyces is well known for their ability to synthesize enormous varieties of antibiotics as secondary metabolites. Among them, S. avermitilis produces avermectins, a group of antiparasitic agents used in human and veterinary medicine. However, S. avermitilis also produces oligomycin, which is a potential toxic inhibitor of oxidative phosphorylation in mammalian cells. Therefore, we decided to disrupt oligomycin synthetase gene to prevent co-production of oligomycin in S. avermitilis. To create plasmid for disruption, the smallest gene of oligomycin synthetase gene cluster was obtained by PCR from S. avermitilis chromosome. Then, apramycin resistance gene was inserted in oligomycin synthetase gene for selection. After transformation of this plasmid, oligomycin synthetase gene (olmA5) in the chromosome was displaced with disruption cassette on the plasmid via homologous recombination. As a result of this gene replacement, we obtained mutants (olmA5::apra) that no longer makes the toxic oligomycin. And the mutants confirmed by PCR and HPLC analysis. However, showed no increasement of avermectin production in the mutant was observed.

• Journal of Climate Change Research
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• v.9 no.4
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• pp.377-384
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• 2018

The chamber method is widely used for measuring methane emission from paddy rice fields. The closed static chamber has advantages of easy installation and removal in the field and low manufacturing cost. However, the manual chamber method requires a lot of labor and has a limited sampling time and frequency. To overcome the disadvantages of the manual chamber, the auto-chamber system is used for measuring methane emission. We compared the differences in methane flux between the auto-chamber and manual chamber. To investigate methane emissions by the two methods, a chamber was installed for each of the following treatments : control without rice straw (NA), spring plowing after autumn rice straw application (SPRA) and autumn plowing after autumn rice straw application (APRA). The total methane emission was lowest in the control and highest in APRA with both methods. There was no significant difference in total methane emission between the methods, but dynamic fluctuation in methane with temperature change was accurately measured in the auto-chamber. Measuring methane emission with an auto-chamber system is expected to reduce uncertainty and increase accuracy, accompanied by labor reduction.

• Transactions of the Korean Society of Automotive Engineers
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• v.19 no.1
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• pp.38-44
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• 2011

Remanufacturing is restoring or manufacturing the worn-out or discarded components of an end of life product in order to bring it to the "like new" condition. The aim is to reprocess used products in such a manner that the quality of the products is as good as or better than the new one, in terms of appearance, reliability and performance. This paper investigates the automotive remanufacturing industry in Europe. To further knowledge in this field, the paper focuses on the remanufacturing of the automotive components of end-of-life vehicles. The paper scope emphasizes key remanufacturing companies, which are identified and were surveyed in terms of their business structures. The research aims to address the potential for growth within the remanufacturing industry, with regard to various players. The state of the art in remanufacturing of automotive equipment will be identified.

• Journal of the Korean Society for Precision Engineering
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• v.27 no.3
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• pp.58-65
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• 2010

Remanufacturing is a process that restores old products to perform like new, while saving energy, reducing consumption of natural resources, and lowering environmental emissions. By extending the product life cycle, remanufacturing approaches enable closed loop material cycles that are ultimately necessary for a sustainable society. This paper provides some description of the current automotive remanufacturing enterprise, with a particular emphasis on key vehicle components that are currently remanufactured. The analysis yields two major conclusions. First, volume of the USA automotive after sales and remanufacturing industry market is estimated. Second, market price of a remanufactured component in the automotive sector is surprisingly uncorrelated with the number of companies engaged in remanufacturing that component - at least for companies registered with the Automotive Parts Remanufacturing Association (ARPA).