• Asian-Australasian Journal of Animal Sciences
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• v.20 no.10
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• pp.1485-1489
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• 2007

Achaete-scute like 2 (ASCL2) gene encodes a member of the basic helix-loop-helix transcription factor which is essential for the maintenance of proliferating trophoblasts during placental development. ASCL2 gene preferentially expresses the maternal allele in the mouse. However, it escapes genomic imprinting in the human. In this study, the complete open reading frame consisting of 193 amino acids of ASCL2 gene was obtained. Sequence analysis indicated that a C-G mutation existed in the 3' region between Meishan and Large White pigs. The polymorphism was used to determine the monoallelic or biallelic expression with RT-PCR-RFLP in pigs of Large $White{\times}Meishan$ $F_1$ hybrids. Imprinting analysis indicated that the ASCL2 gene expression was biallelic in all the tested tissues (heart, liver, spleen, lung, kidney, stomach, small intestine, skeletal muscle, fat, uterus, ovary and pituitary). PCR-RFLP was used to detect the polymorphism in 270 pigs of the "$Large\;White{\times}Meishan$" $F_2$ resource population. The statistical results showed highly significant associations of the genotypes and fat meat percentage (FMP), lean meat percentage (LMP) and ratio of lean to fat (RLF) (p<0.01), and significant associations of the genotypes and loin eye area (LEA) and internal fat rate (IFR) (p<0.05).

• The Korean Journal of Vision Science
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• v.20 no.4
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• pp.469-482
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• 2018

Purpose : We investigated the change of spherical and comma aberrations after wearing aspheric soft contact lens (ASCL) in young myopes. Methods : Fifty young myopes ($23.15{\pm}1.70years$, spherical equivalent: $-2.90{\pm}1.75D$) were recruited and refractive errors were corrected using ASCL (Biotrue, Bausch+Lomb, USA). High order aberrations were measured in the 4 mm pupil size using the wavefront analyze and pupil sizes were measured with a pupillometer at the modes of scotopic condition (light off) at 3.5 m in the 100 lx illuminance condition. Results : Spherical aberrations and coma aberration of the 20s myopes were $0.026{\pm}0.031{\mu}m$ and $0.078{\pm}0.039{\mu}m$ respectively, and $0.019{\pm}0.026{\mu}m$ and $0.082{\pm}0.038{\mu}m$ after ASCL wear that spherical aberration was decreased and coma aberration was increased. However, spherical aberration was decreased in the 68% of the subject have positive spherical aberration, and increased in the 11% of the subject have negative spherical aberration. Coma aberration was increased in the 53% of the subject, did not change in the 19% of the subjects, and decreased in the 28% of the subject. Spherical aberration was not different with the refractive errors in low and moderate myopies, however, coma aberrations was higher in the higher myopes. Conclusion : In a scotopic condition without accommodation stimuli, spherical aberration is decreased after wearing ASCL, however in the subject have negative spherical aberration spherical aberration could be increased, and which is thought to be the influence of contact lens design and pupil size.

• The Transactions of the Korean Institute of Power Electronics
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• v.2 no.3
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• pp.11-19
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• 1997

본 논문에서는 스위치드 리럭턴스 전동기의 토크 리플 또는 소음을 감소하기 위한 방법으로써 순시 전압제어방식을 제시하였다. 이 방식은 상 정류의 제곱의 합ㅇ르 제어하여 토크 리플을 제거할 수 있다. 제안방식에 대해 해석하였으며, 이 방식의 이론적인 타당성을 입증시키기 위해 소프트웨어 페키지 ASCL에 의해 시뮬레이션하였다. 실험에 의해 SRM 토크 리플의 제거를 확인하였다.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.45 no.2
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• pp.114-123
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• 2017

Little Intelligent Nanosatellite of KAIST(LINK) is a 2U-size CubeSat which is developed by Aerospace Systems & Control Lab.(ASCL) of KAIST as a part of the international cooperation project QB50. The objective of the QB50 project is to carry out atmospheric research within the lower thermosphere and ionosphere and CubeSats are planned to be deployed at the International Space Station(ISS) from the first quarter of 2017. To implement this objective, a flight model(FM) of LINK has been successfully developed and the design and performance of the satellite have been verified by performing environment and function tests in accordance with acceptance requirement level. This paper describes the development of flight model and the results of vibration and thermal vacuum test.

• Biomolecules & Therapeutics
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• v.31 no.3
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• pp.264-275
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• 2023

Parkinson's disease (PD) is a common neurodegenerative disorder characterized by tremors, bradykinesia, and rigidity. PD is caused by loss of dopaminergic (DA) neurons in the midbrain substantia nigra (SN) and therefore, replenishment of DA neurons via stem cell-based therapy is a potential treatment option. Astrocytes are the most abundant non-neuronal cells in the central nervous system and are promising candidates for reprogramming into neuronal cells because they share a common origin with neurons. The ability of neural progenitor cells (NPCs) to proliferate and differentiate may overcome the limitations of the reduced viability and function of transplanted cells after cell replacement therapy. Achaete-scute complex homolog-like 1 (Ascl1) is a well-known neuronal-specific factor that induces various cell types such as human and mouse astrocytes and fibroblasts to differentiate into neurons. Nurr1 is involved in the differentiation and maintenance of DA neurons, and decreased Nurr1 expression is known to be a major risk factor for PD. Previous studies have shown that direct conversion of astrocytes into DA neurons and NPCs can be induced by overexpression of Ascl1 and Nurr1 and additional transcription factors genes such as superoxide dismutase 1 and SRY-box 2. Here, we demonstrate that astrocytes isolated from the ventral midbrain, the origin of SN DA neurons, can be effectively converted into DA neurons and NPCs with enhanced viability. In addition, when these NPCs are inducted to differentiate, they exhibit key characteristics of DA neurons. Thus, direct conversion of midbrain astrocytes is a possible cell therapy strategy to treat neurodegenerative diseases.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.12 no.2
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• pp.157-157
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• 1998

이 논문은 브리시리스 직류전동기의 구동 인버터의 실시간 데이터를 이용한 고장진단에 관한 것이다. 구동 인버터의 고장유형을 파악하여 주요 고장증세별로 분류하고, 고장결과를 예측하여 ASCL로 시뮬레이션함으로써 지식 베이스로 구성하였다. 구동 인버터에 대해 실시간으로 감시된 데이터는 전문가 시스템의 추론기관에서 시뮬레이션된 지식베이스와 비교하게 된다. 고장이 발생하면, 운전을 중지시킨 후, 전문가 추론을 함으로써 고장원인을 진단한다. 이로써 구동 인버터에 대해 전문적인 지식을 갖고 있지 않는 사용자에게, 고장원인 제거 및 수리대책에 관한 전문가의 지식을 신속히 제공하는 것이다.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.12 no.2
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• pp.29-37
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• 1998

This paper describes the fault diagnosis based on real-time data of the inverter system for brush less DC motor drive. After identifying all the fault types in the inverter system, a preliminary typical analysis of fault types has been classified into the key fault symptoms. The predicted fault performances are then substantiated by using ACSL(Advanced Continuous Simulation Language), and the simulated results are composed of knowledge-base. The real-time measured data from the inverter system are compared with the simulated knowledge-base through the inference engine of expert system, which have been used to diagnose the fault causes. If some faults may occur in the inverter system, this system will be stopped. And then the expertise of elimination and remedial strategies about the fault causes, will be supplied rapidly to operator who doesn't know well about the inverter drive system.system.

• Genomics & Informatics
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• v.8 no.4
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• pp.185-193
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• 2010

Histone deacetylation and demethylation are epigenetic mechanisms implicated in cancer. Studies regarding the role of modulation of gene expression utilizing the histone deacetylase inhibitor scriptaid and the demethylating agent 5-azacytidine in HL-60 leukemia cells have been limited. We studied the possibility of recovering epigenetically silenced genes by scriptaid and 5-azacytidine in human leukemia cells by DNA microarray analysis. The first group was leukemia cells that were cultured with 5-azacytidine. The second group was cultured with scriptaid. The other group was cultured with both agents. Two hundred seventy newly developed genes were expressed after the combination of 5-azacytidine and scriptaid. Twenty-nine genes were unchanged after the combination treatment of 5-azacytidine and scriptaid. Among the 270 genes, 13 genes were differed significantly from the control. HPGD, CPA3, CEACAM6, LOC653907, ETS1, RAB37, PMP22, FST, FOXC1, and CCL2 were up-regulated, and IGLL3, IGLL1, and ASS1 were down-regulated. Eleven genes associated with oncogenesis were found among the differentially expressed genes: ETS1, ASCL2, BTG2, BTG1, SLAMF6, CDKN2D, RRAS, RET, GIPC1, MAGEB, and RGL4. We report the results of our leukemia cell microarray profiles after epigenetic combination therapy with the hope that they are the starting point of selectively targeted epigenetic therapy.

• Molecules and Cells
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• v.40 no.6
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• pp.426-433
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• 2017

Ephrin-A5 has been implicated in the regulation of brain morphogenesis and axon pathfinding. In this study, we used bacterial homologous recombination to express a LacZ reporter in various ephrin-A5 BAC clones to identify elements that regulate ephrin-A5 gene expression during mesencephalon development. We found that there is mesencephalon-specific enhancer activity localized to a specific +25.0 kb to +30.5 kb genomic region in the first intron of ephrin-A5. Further comparative genomic analysis indicated that two evolutionary conserved regions, ECR1 and ECR2, were present within this 5.5 kb region. Deletion of ECR1 from the enhancer resulted in disrupted mesencephalon-specific enhancer activity in transgenic embryos. We also found a consensus binding site for basic helix-loop-helix (bHLH) transcription factors (TFs) in a highly conserved region at the 3'-end of ECR1. We further demonstrated that specific deletion of the bHLH TF binding site abrogated the mesencephalon-specific enhancer activity in transgenic embryos. Finally, both electrophoretic mobility shift assay and luciferase-based transactivation assay revealed that the transcription factor Ascl1 bound the bHLH consensus binding site in the mesencephalon-specific ephrin-A5 enhancer in vitro. Together, these results suggest that the bHLH TF binding site in ECR1 is involved in the positive regulation of ephrin-A5 gene expression during the development of the mesencephalon.