• Korean Journal Plant Pathology
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• v.14 no.2
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• pp.136-144
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• 1998

Many cloves of native cultivated garlics in Korea were found to be infested by mites when observed with stereo-microscope. The mite was identified by light and scanning electron microscopic observation as Aceria tulipae. Surveying viruses from the vegetatively propagated garlic, highly flexuous, filamentous particles (700∼800 nm) were detected in Aceria tulipae, local lesions of Chenopodium murale after sap transmissions, mosaic garlic leaves inoculated with mite-borne virus by transmission of Aceria tulipae and naturally infected garlic leaves. The mite-borne virus isolated did not react with antisera of aphid-borne potyviruses (LYSV-G, LYSV-L, WoYSV) or carlavirus (GLV), but reacted with antisera of garlic mite-borne viruses (GV-C, GMbMV). In ultratin sections of mite-borne virus infected garlic tissues, aggregates of virus particles and membrane proliferations were found in the parenchyma cells, but cytoplasmic cylindrical inclusions were not observed. Heavily mite-infested plants showed streaking and malformation due to mite feeding. The mite-borne virus was identified as garlic mite-borne mosaic virus (GMbMV), the mite-borne genus Rymovirus of the Potyviridae by mite transmission, morphology of virus particles, serological relationships, host range, distribution pattern of virus particles and inclusion bodies in the infected cells. The results demonstrate that mite-borne virus is one of the major viruses infecting native cultivated garlic plants showing mosaic or streak symptoms in Korea.

• Korean journal of applied entomology
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• v.37 no.1
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• pp.81-89
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• 1998

In a survey on the pests of stored garlic, an unrecorded gall mite in Korea was found to damage on garlic surface making stripe feeding injuries. The mite was identified as Aceria tulipae (Keifer) 1938, based on the morphological characteristics. Light microscopic and SEM observations on garlics fed by A. tulipae revealed that there was no gall formation by feeding but there was only surface damage symptom. The damaged garlic surface showed symptom of losing gloss in the early stage and the feeding traces under dissecting microscope. Brown stripes appeared in the middle stage of damage and the whole scale turned to dark brown and shrank in the later stage. When the mite infested garlics were fumigated with 3 g/m3 aluminium phosphide for 72 hours in airtight plastic boxes (1 m x 1 m x 1 m), the mortalities of mite were over 90% for Namdo garlics.

• Korean journal of applied entomology
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• v.37 no.1
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• pp.65-71
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• 1998

Survey was conducted on the kinds and densities of pests associated with stored garlic collected from farms of major garlic production areas from 1994 to 1995 in Korea. Aceria tulipae, Rhizoglyphus sp., Ditylenchus dipsaci, and Tyrophagus putrescentiae were frequently detected with high densities and Tarsonemus bilobatus and Aphelenchus avenue showed relatively low detection rates and densities. Detection rate of A. tulipue was 38% in 1994, but the rate was 65% in 1995. Number of the mite ranged from 1 to 4,500 per scale. Detection rate of Rhizoglyphus sp. was 63% in 1994, but the rate was 13% in 1995 and average number of the mite ranged from 1 to 135 per scale in 1994. Garlics damaged by Rhizoglyphus sp. showed decaying symptom. T~rophagusp utrescentiae was detected from 22 farms among 32 farms surveyed in 1994 and from 21 farms among 39 farms surveyed in 1995. However, number of the mite on garlic scale was relatively lower than the other mites and its damages on stored garlic was not determined.

• Journal of Microbiology and Biotechnology
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• v.17 no.11
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• pp.1833-1840
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• 2007

Viruses in garlic plants (Allium sativum L.) have accumulated and evolved over generations, resulting in serious consequences for the garlic trade around the world. These viral epidemics are also known to be caused by aphids and eriophyid mites (Aceria tulipae) carrying Potyviruses, Carlaviruses, and Allexiviruses. However, little is known about viral epidemics in garlic plants caused by eriophyid mites. Therefore, this study investigated the infection of garlic plants with Allexiviruses by eriophyid mites. When healthy garlic plants were cocultured with eriophyid mites, the leaves of the garlic plants developed yellow mosaic strips and became distorted. In extracts from the eriophyid mites, Allexiviruses were observed using immunosorbent electron microscopy (ISEM). From an immunoblot analysis, coat proteins against an Allexivirus garlic-virus antiserum were clearly identified in purified extracts from collected viral-infected garlic plants, eriophyid mites, and garlic plants infected by eriophyid mites. A new strain of GarV-B was isolated and named GarV-B Korea isolate 1 (GarV-B1). The ORF1 and ORF2 in GarV-B1 contained a typical viral helicase, RNA-directed RNA polymerase (RdRp), and triple gene block protein (TGBp) for viral movement between cells. The newly identified GarV-B1 was phylogenetically grouped with GarV-C and GarV-X in the Allexivirus genus. All the results in this study demonstrated that eriophyid mites are a transmitter insect species for Allexiviruses.

• The Plant Pathology Journal
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• v.18 no.5
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• pp.237-243
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• 2002

A survey of virus infection in garlic plants cultivated in Korea was conducted for three years. Most virus-infected garlic plants (Allium sativum) showed typical symptoms on the leaves such as yellow mosaic, stripes, and distortion. Through immunosorbent electron micro-scopy and RT-PCR analysis, the complex mixtures of viruses including garlic viruses of the genus Allerivirus, gaylic strain of Leek yellow stripe virus of the genus Potyvirus, and Garlic latent virus of the genus Carlavirus were identified in the virus-infected garlic plants. Among these viruses, Allexivirus was the most frequently detect-ed in the regions surveyed. Using sets of differential primers for Allexivirus genomes, two members of the genus were amplified and sequenced from the purified viruses. The deduced amino acid sequences for the coat proteins and the nucleic acid binding proteins of two viruses showed high homologies to Garlic virus A (CarV-A) and Garlic virus D (GarV-D) of Allekivirus. This is the first report of GarV-A and GarV-D in Korea. This suggests that Allexivirus in gavlic plants in Korea was mixed and varied. Phylogenetic analyses showed that the genus Allexivirus was diversi(ied by the processes of accumulation and evolution of viruses in garlic plants due to the long period of repeated vegetative propagation.