• Journal of the Korean Chemical Society
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• v.37 no.12
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• pp.1060-1067
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• 1993

A series of New N-[1-(benzotriazol-1-yl)-X-substituted benzyl]-Y-substituted aniline derivaties (S) have been synthesized. And the rate of hydrolysis was investigated kinetically in 25% (v/v) aqueous methanol at 25$^{\circ}C$. On the basis of rate equations, solvent effect $m {\ll} 1,\; n \leq 3\; and\; m {\ll} l$), salt effect, general base catalysis, substituent effect (${\rho}_{xy}$ > > 0), and hydrolysis products analysis, it may be concluded that the hydrolysis of N-[1-(benzotriazol-1-yl)benzyl]aniline proceeds the "A-$S_N2$" mechanism below pH 12.0, while above pH 13.0, the hydrolysis proceeds through a typical "$S_N2$" mechanism.

• Journal of Microbiology
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• v.43 no.6
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• pp.475-486
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• 2005

Fungal secondary metabolites constitute a wide variety of compounds which either playa vital role in agricultural, pharmaceutical and industrial contexts, or have devastating effects on agriculture, animal and human affairs by virtue of their toxigenicity. Owing to their beneficial and deleterious characteristics, these complex compounds and the genes responsible for their synthesis have been the subjects of extensive investigation by microbiologists and pharmacologists. A majority of the fungal secondary metabolic genes are classified as type I polyketide synthases (PKS) which are often clustered with other secondary metabolism related genes. In this review we discuss on the significance of our recent discovery of chalcone synthase (CHS) genes belonging to the type III PKS superfamily in an industrially important fungus, Aspergillus oryzae. CHS genes are known to playa vital role in the biosynthesis of flavonoids in plants. A comparative genome analyses revealed the unique character of A. oryzae with four CHS-like genes (csyA, csyB, csyC and csyD) amongst other Aspergilli (Aspergillus nidulans and Aspergillus fumigatus) which contained none of the CHS-like genes. Some other fungi such as Neurospora crassa, Fusarium graminearum, Magnaporthe grisea, Podospora anserina and Phanerochaete chrysosporium also contained putative type III PKSs, with a phylogenic distinction from bacteria and plants. The enzymatically active nature of these newly discovered homologues is expected owing to the conservation in the catalytic residues across the different species of plants and fungi, and also by the fact that a majority of these genes (csyA, csyB and csyD) were expressed in A. oryzae. While this finding brings filamentous fungi closer to plants and bacteria which until recently were the only ones considered to possess the type III PKSs, the presence of putative genes encoding other principal enzymes involved in the phenylpropanoid and flavonoid biosynthesis (viz., phenylalanine ammonia-lyase, cinnamic acid hydroxylase and p-coumarate CoA ligase) in the A. oryzae genome undoubtedly prove the extent of its metabolic diversity. Since many of these genes have not been identified earlier, knowledge on their corresponding products or activities remain undeciphered. In future, it is anticipated that these enzymes may be reasonable targets for metabolic engineering in fungi to produce agriculturally and nutritionally important metabolites.

• Atmosphere
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• v.16 no.2
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• pp.85-96
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• 2006

The six-year (2000~2005) record of aerosol optical thickness (AOT or $\tau$) data from the Moderate Resolution Imaging Spectroradiometer (MODIS) was analyzed over the Northeast Asia. The MODIS AOT standard products (MOD04_L2) over both ocean and land were collected to evaluate the spatial and temporal variability of the atmospheric aerosols over the study region ($32^{\circ}N{\sim}42^{\circ}N$ and $115^{\circ}E{\sim}133^{\circ}E$). The monthly averaged AOT result revealed slight changes(${\pm}0.002{\tau}/month$), which was almost unchangeable, over Korea. In contrast, the large AOT values (> 0.6) and a significant AOT increase (> 0.004 ${\tau}/month$) over East China were observed. For the analysis of spatio-temporal variability of AOT values, study area was divided by six sectors (I: North-East China, II: East China, III: Yellow Sea, IV: Korea Peninsular, V: East Sea, and VI: South Sea and Western part of Japan). The considerable result showed that particularly high AOT contribution was observed over sector I (32.5%) and II (25.5%) where some major urban and industrialized areas and agricultural fields are located and other cases were observed 13.2%, 14.6%, 7.1%, 7.0% over sector III, IV, V, and VI, respectively. In addition, yearly AOT changes based on seasons are observed differently at each sector but increasing trends reveal in summer and fall over all sectors.

• THE INTERNATIONAL COMMERCE & LAW REVIEW
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• v.47
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• pp.379-402
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• 2010

The Korea Foreign Trade Act(KFTA) was revised the penal provisions of the breach of the Country of Origin Labeling(COOL) recently. The ceiling of penalties became to 5 years for imprisonment, one hundred or three hundred million won for fine. The level of penalties are adjudged quite fair but the amount of penalty should be increased according to the profits from the breach or the nature of crime in some cases. The problems of the penalties are differences between KFTA and other related laws. There are related several laws on the breach of the COOL such as KFTA, Unfair Trade related Law, Customs Law, Consumer Protection Law, Law of COOL on Agricultural and Marine products etc. The penal provisions of the breach of the COOL has more heavier level than other the breach because of the criminal qualities. The problems are the penalty differences between the KFTA and the Unfair Trade Law under the Ministry of Knowledge Economy. The KFTA's penal provisions need to equate with Unfair Trade Law as long as same breaches on the COOL. The government can also consider some policies to rigid enforcement of breaches on the COOL. There are the Country of Origin Tracking system, the RoO Paparazzi System, Make public the names of habitual RoO Violators, Correction Order of breach of the COOL etc.

• Journal of Microbiology and Biotechnology
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• v.26 no.2
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• pp.248-254
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• 2016

A soil metagenome contains the genomes of all microbes included in a soil sample, including those that cannot be cultured. In this study, soil metagenome libraries were searched for microbial genes exhibiting lipolytic activity and those involved in potential lipid metabolism that could yield valuable products in microorganisms. One of the subclones derived from the original fosmid clone, pELP120, was selected for further analysis. A subclone spanning a 3.3 kb DNA fragment was found to encode for lipase/esterase and contained an additional partial open reading frame encoding a wax ester synthase (WES) motif. Consequently, both pELP120 and the full length of the gene potentially encoding WES were sequenced. To determine if the wes gene encoded a functioning WES protein that produced wax esters, gas chromatography-mass spectroscopy was conducted using ethyl acetate extract from an Escherichia coli strain that expressed the wes gene and was grown with hexadecanol. The ethyl acetate extract from this E. coli strain did indeed produce wax ester compounds of various carbon-chain lengths. DNA sequence analysis of the full-length gene revealed that the gene cluster may be derived from a member of Proteobacteria, whereas the clone does not contain any clear phylogenetic markers. These results suggest that the wes gene discovered in this study encodes a functional protein in E. coli and produces wax esters through a heterologous expression system.

• Journal of Microbiology and Biotechnology
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• v.23 no.2
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• pp.161-166
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• 2013

At present, acetylcholinesterase (AChE) inhibitors are the first group of drugs to treat mild to moderate Alzheimer's disease (AD). Although beneficial in improving cognitive and behavioral symptoms, the effectiveness of AChE inhibitors has been questioned since they do not delay or prevent neurodegeneration in AD patients. Therefore, in the present study, in order to develop new and effective anti-AD agents from lichen products, both the AChE inhibitory and the neuroprotective effects were evaluated. The AChE inhibitory assay was performed based on Ellman's reaction, and the neuroprotective effect was evaluated by using the MTT method on injured PC12 cells. One AChE inhibitor ($IC_{50}$ = 27.1 ${\mu}g/ml$) was isolated by means of bioactivity-guided isolation from the extract of lichen-forming fungus Cladonia macilenta, which showed the most potent AChE inhibitory activity in previous screening experiment. It was then identified as biruloquinone by MS, and $^1H$- and $^{13}C$-NMR analyses. The inhibitory kinetic assay suggested that biruloquinone is a mixed-II inhibitor on AChE. Meanwhile, biruloquinone improved the viability of the $H_2O_2$- and ${\beta}$-amyloid-injured PC12 cells at 1 to 25 ${\mu}g/ml$. The protective effects are proposed to be related to the potent antioxidant activities of biruloquinone. These results imply that biruloquinone has the potential to be developed as a multifunctional anti- AD agent.

• Korean Journal of Food Science and Technology
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• v.7 no.2
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• pp.96-99
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• 1975

In order to establish an optimal flour milling method for naked barley, the tempering conditions, milling after pearling and mixed flour milling of naked barley with wheat were studied, and the following results were obtained. 1. For the tempering of naked barley, treatment for 48 hours at a moisture level of 13. 5% and addition of 0. 5% water prior to the flour milling is the most useful procedure. 2. The pearling of naked barley before or after tempering lowers the ash content in the flour, but the yield is reduced considerably and two steps of processing make the procedure unsuitable. 3. For the mixed flour milling, the mixing ratio of naked barley to wheat ranging from 10 : 90 to 20 : 80 is optimal.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 2003.10a
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• pp.226.2-227
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• 2003

The degree of milling is an important factor in terms of the nutritional value and the economic return of the milled rice. This study was to investigate the changes in compositions of the oils obtained from rice with different degree of milling. The content of total tocols in rice significantly (p＜0.05) decreased, as degree of milling increased. The relative ％ of -tocopherol in brown rice had lower than those in milled rices whereas the relative ％ of -tocotrienol, and -tocotrienol in brown rice higher than those in milled rices. -Oryzanol content significantly (P<0.05) decreased from 198.5 mg/kg rice for brown rice (0% milling) to 65.5 125.0 mg/kg rice for rice samples with 5.6, 8.0, and 9.6 ％ milling. -Sitosterol was most abundant sterol representing 50 ％ - 56% of the total sterol content in all analyzed samples. The content of total sterol as well as each sterol isomers in rice significantly (P<0.05) decreased, as degree of milling increased. A similar trend was observed in changes of octacosanol and squalene.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 2003.10a
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• pp.187-187
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• 2003

To examine the effect of packaging method on strawberry, tomato, and plum quality, the rate of weight loss, Hunter a value, decay rate, anthocyanin contents, and microbial (total bacterial counts, mold and yeast, and pseudomonas) changes were determined during storage. Strawberry was packaged with low density polyethylene (LDPE). Tomato and plum were packaged with high density polyethylene film (HDPE). Strawberries, tomatoes, and plums were then stored at 4$^{\circ}C$ and 20$^{\circ}C$, respectively. LDPE package was the most effective on the decrease of decay rate of strawberry and the rate of weight loss for packaged strawberry was lower than that of the non-packaged. HDPE package was the most effective on the rate of weight loss during storage of tomatoes and plums regardless of storage temperature. Hunter a value increased during storage. Anthocyanin contents of plums increased overall with increasing storage time, and plums stored without package were changed more than those with package. Microbial changes of strawberry, tomato, and plum stored at 4$^{\circ}C$ and 20$^{\circ}C$ were monitored during storage. Packaging method did not affect the microbial change, yet temperature did affect the microbial change significantly. These results indicate that storage of these commodities at 4$^{\circ}C$ should be recommended in terms ,of microbial safety as well as quality and shelf-life.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 2003.10a
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• pp.174-175
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• 2003

현재 유통되고 있는 일반백미의 경우 소비자가 밥을 짓기 위해서는 쌀을 씻어야하는 번거로움 뿐만아니라 쌀 중량의 약 15배의 물이 소요되고, 또한 이때 발생된 쌀뜨물이 수질오염의 원인이 되기 때문에 무세미가공 및 보급 필요성이 점차 높아지고 있다. 무세미 가공분야의 선진국인 일본의 경우 ‘92년부터 습식 무세미조제기가 실용화되기 시작, 현재 사다께 등 6개사에서 기계장치를 생산보급 하고 있으며, 무세미의 유통도 일반화되어가고 있는 추세이다. 국내의 경우 무세미조제기는 근래에서야 습식 무세미 조제설비가 국산화되어 보급초기 단계 있으며, 무세미의 안정적 가공 및 보급을 위해서는 앞으로 많은 연구가 이루어져야 할 것으로 생각된다. 특히 습식의 경우 쌀이 물에 접촉하는 시간이 길 경우 품질저하의 우려가 크고, 쌀중량의 1.4배에 해당하는 물이 가공과정에 필요하기 때문에 물사용량을 억제할수 있는 가공기술의 개발 필요성이 커지고 있다. 따라서 본 연구에서는 물사용량을 최소화 할 수 있는 무세미 조제시스템을 개발하고자 하였으며, 주요결과를 요약하면 다음과 같다. 시작기는 연마 및 공기세척부, 정전기 세척부, 미세가수세척부로 구성하여 쌀이 단계적으로 세척될 수 있도록 제작되었다. 성능시험 결과, 각 세척공정별 세척수의 탁도 감소효과는 연마 및 공기크리닝부에서 22.67ppm, 정전기 크리닝부에서 8.33ppm, 미세가수세척부에서 17.34 ppm이 감소되는 것으로 나타났다. 특히 정전기세척부의 탁도감소 효과는 다른 두공정에 비해 작지만 제거가 쉽지 않은 미세한 쌀겨들을 제거하여 탁도를 개선을 시켰기 때문에 미세가수세척장치에서 가수량을 적게 사용하는데 기여한 것으로 판단된다. 가공시 적정 탁도를 확보할 수 있는 가수량은 430cc/kg로 기존의 습식에 비해 약 69%정도 세척수 절감효과가 있었다. 이때 미세가수세척부의 원통스크린 회전수는 108, 205rpm범위가 적정한 것으로 나타났다. 쌀의 품위는 탁도가 가공전 97.33ppm(일반백미)에서 가공후 최대 48.00ppm으로 낮아졌으며, 백도도 가공전 36.80에서 42.80으로 향상되어 씻지 않고도 밥을 지을수 있는 무세미 가공이 가능하였다. 이밖에 쇄립률은 가공전 5.30%에서 7.37%로 다소 증가하는 것으로 나타났고, 함수율은 가공전 15.60%에서 15.80%로 약 0.2%가 증가하였으나 기존의 연구결과에 비춰볼 때 문제가 되지 않을 것으로 여겨진다.