• Journal of Applied Biological Chemistry
• /
• 제43권4호
• /
• pp.207-210
• /
• 2000

As a typical example of the screening for a microbial biocatalyst from nature, isolation of nitrilesynthesizing microorganisms, characterization of a new enzyme aldoxime dehydratase, and its function in the aldoxime-nitrile pathway are introduced. Catalytic properties of some of our enzymes were improved through a direct evolutionary approach.

• Bulletin of the Korean Chemical Society
• /
• 제33권10호
• /
• pp.3173-3174
• /
• 2012

• Bulletin of the Korean Chemical Society
• /
• 제26권6호
• /
• pp.975-978
• /
• 2005

• BMB Reports
• /
• 제31권3호
• /
• pp.296-302
• /
• 1998

A potentiometric flow injection biosensor for the analysis of anti-cholinesterases (anti-ChEs), based on inhibition of enzyme activity, was developed. The sensor system consists of a reactor with acetylcholinesterase (AChE) immobilized on controlled pore glass and a detector with an $H^{+}-selective$ PVC-based membrane electrode. The principle of the analysis is based on the fact that the degree of inhibition of AChE by an anti-ChE is dependent on the concentration of the anti-ChE in contact with AChE. The sensor system was optimized by changing systematically the operating parameters of the sensor to evaluate the effect of the changes on sensor response to ACh. The optimized biosensor was applied to the analysis of paraoxon, an organophosphorus pesticide. Treatment of the inhibited enzyme with pyridine-2-aldoxime fully restored the enzyme activity allowing repeated use of the sensor.

• 대한화학회지
• /
• 제52권1호
• /
• pp.52-56
• /
• 2008

제법을 위한 쉽고 효과적인 이 방법은 solvent-free하에서 H3PMo12O40 촉매를 사용해서 개선되었다. 이 방법의 큰 이점은 조작의 간편성, 더 작은 촉매량, 선택성, 온화한 반응조건,짧은 반응 시간 그리고 높은 수율이다. 재생된 촉매는 어떤 정제도 없이 다시 사용되어질 수 있다.

• Applied Biological Chemistry
• /
• 제32권4호
• /
• pp.401-407
• /
• 1989

생리활성을 갖는 신규물질 탐색의 일환으로 1,3 치환 -5-chloropyrazole-4-carbonyl chloride를 합성한 뒤 8종의 aldoxime 또는 ketoxime과 친핵 반응시켜 15종의 pyrazole-oxime ester를 얻었으며, 이들의 구조를 $^1H,\;^{13}C-NMR$ 및 IR 등으로 확인하였다. 이 유도체들의 R. solani, P. oryzae, B. cinerea, P. graminearum, P. capsici 및 G. cingulata에 대한 균사생육억제효과를 검정한 결과, oxime 부위$(R^3)$가 친전자성기로 치환된 방향족인 경우가(III, XIII, XIV) 포화알킬기인 경우보다 우수한 효과를 보여주었다.

• 한국군사과학기술학회지
• /
• 제20권4호
• /
• pp.587-596
• /
• 2017

In this study, multi-chambered single autoinjector(2in1) and KMARK-1 containing atropine and 2-PAM(pyridine-2-aldoxime methylchloride) were administered to the beagle's muscle, and blood samples were taken for a certain period of time to compare and evaluate the pharmacokinetic profiles of the two drugs. Male beagles were used and classified into two test groups(G1, G2), and crossover pharmacokinetic studies were performed in two test groups. Blood samples were collected from the jugular vein for analysis after administration. The 90 % confidence interval(CI) for log transformed data indicated that the Cmax for both atropine(log 0.9683 ~ log 1.113) and 2-PAM(log 0.9453 ~ log 1.214) was within the limits of bioequivalence criteria, but the AUC for atropine(log 1.1786 ~ log 1.3238) failed to meet this criteria. This is expected as the amount of atropine dose is 25 % higher for the test as compared to the reference formulation. In summary, in view of the ATNAA(antidote for nerve agent of US) authorization, the Cmax equivalence was more important than AUC equivalence, so in this study, we also focused on verifying the equality of Cmax between the two autoinjectors.