• Animal cells and systems
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• 제4권1호
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• pp.77-85
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• 2000

This study has demonstrated the molecular variation of 5S rRNA genes in 15 Allium subgenus Rhizirideum and 1 Allium subg. Allium. For cloning of the 5S rRNA genes, PCR products were obtained from amplification with oligonucleotide primers which were derived from the conserved coding region of 5S rRNA genes. These amplified PCR products were cloned and identified by FISH and sequence analysis. The 5S rRNA loci were primarily located on chromosomes 5 and/or 7 in diploid species and various chromosomes in alloploid species. The size of the coding region of 5S rRNA genes was 120 bp in all the species and the sequences were highly conserved within Allium species. The sizes of nontranscribed spacer (NTS) region were varied from 194 bp (A. dektiude-fustykisum, 2n=16) to 483 bp (A. sativum). Two kinds of NTS regions were observed in A. victorialis var. platyphyllum a diploid, A. wakegi an amphihaploid, A. sacculiferum, A. grayi, A. deltoide-fistulosum and A. wenescens all allotetraploids, while most diploid species showed only one NTS region. The species containing two components of NTS region were grouped with different diploid species in a phylogenetic tree analysis using the sequences of 5S rRNA genes and adjacent non-coding regions.

• Journal of Plant Biotechnology
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• 제47권1호
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• pp.15-25
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• 2020

Allium L. is one of the largest genera of the Amaryllidaceae family, with more than 920 species including many economically important species used as vegetables, spices, medicines, or ornamental plants. Currently, DNA barcoding tools are being successfully used for the molecular taxonomy of Allium. A total of 46 Allium species were collected from their native areas, and DNA was extracted using the IBRC DNA extraction kit. We used specific primers to PCR amplify matK. DNA sequences were edited and aligned for homology, and a phylogenetic tree was constructed using the neighbor-joining method. The results show thymine (38.5%) was the most frequent and guanine (13.9%) the least frequent nucleotide. The matK regions of the populations were quite highly conserved, and the amount of C and CT was calculated at 0.162 and 0.26, respectively. Analysis of the nucleotide substitution showed C-T (26.22%) and A-G (8.08%) to have the highest and lowest percent, respectively. The natural selection process dN/dS was 1.16, and the naturality test results were -1.5 for Tajima's D and -1.19 for Fu's Fs. The NJ dendrogram generated three distinct clades: the first contained Allium austroiranicum and A. ampeloprasum; the second contained A. iranshahrii, A. bisotunense, and A. cf assadi; and the third contained A. rubellum and other species. In this study, we tested the utility of the matK region as a DNA barcode for discriminating Allium. species.

• 식물분류학회지
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• 제51권2호
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• pp.141-146
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• 2021

Allium stenodon (Amaryllidaceae) is reported as a returning member of the Korean flora. This species proved to be the only member of the section Sikkimensia among Korean Allium species, and it is easily distinguished by its cylindrical bulbs with fibrous to sometimes subreticulate tunics and blue to purple-blue perianth. Previously recognized A. baekdusanense is treated as an additional synonym of A. stenodon. Photographs and complete descriptions of A. stenodon are provided together with a dichotomous key to the Korean Allium species. In addition A. stenodon is newly recorded in Jilin province in China.

• 식물분류학회지
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• 제33권3호
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• pp.295-301
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• 2003

한반도 중부지방의 강변을 따라 생육하는 부추속(부추과) 1분류군을 국내 미기록종으로 보고한다. 이 분류군은 지금까지 중국에만 분포하는 것으로 알려졌던 Allium longistylum Baker로서, 국내 생육지의 특성을 고려하여 국명을 '강부추로' 신청하였다. 이 분류군에 대한 형태적 특징을 기재하였고, 도해 및 생태 사진을 제시하였다.

• Journal of Forest and Environmental Science
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• 제27권1호
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• pp.33-37
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• 2011

This study was carried out to establish a proper cultivation site and diagnose the drought-tolerance of Allium ochotense leaves and Allium microdictyon leaves by using pressure - volume curve method. The Allium microdictyon leaves had more lower osmotic water potential ${\psi}osat$, and osmotic water potential at turgor loss point ${\psi}otlp$ than Allium ochotense leaves. Then, the Allium microdictyon leaves was slightly higher drought-tolerance than Allium ochotense leaves. Therefore, We suggest that growth of the two Allium species was appropriate for relative moisture forest.

• Journal of Plant Biology
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• 제20권2호
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• pp.71-76
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• 1977

The present paper was carried out to clear up the polyploidal constitution and the banding pattern of three species in tetraploidal Allium(X=8) through the studies of meiosis, karyotype and G-bands. From the meiotic data and G-banding patterns obtained under this study, it is confirmed that A. tuberosum and A. chinense are autotetraploids, and A. senescens is allotetraploid. Some aneuploids out of the employed species were found; A. senescens is 2n=34, and A. chinense is 2n=33. The chromosome types of these species are meta-and submetacentrics except the sat-chromosomes and the f-chromosomes. G-bands of these species are generally located in the end of each arm. A. senescens is similar in the quantity of heterochromatin with A. chinense, but A. tuberosum has a little than the other species. The quantity of heterochromatin is higher in small groups of chromosome than large ones, and higher in short arms than long arms.

• 동아시아식생활학회지
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• 제11권1호
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• pp.33-43
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• 2001

The effects of the addition of herbs belonging to Allium species on the quality and storage characteristics of kamaboko were studied. The herbs employed in the study were garlic (Allium sativum for.Pekinenese Makino), leek(.Allium tuberosum Roth), onion(Allium cepa Linnaeus), and onion skin. water activity(Aw) , pH, TBA, VBN, microbial load, textural characteristics and sensory evaluation were tested. 1. Aw of all the samples decreased on storage, the Aw on lower day ranged from 0.937~o.950. All thesamples containing herbs retained the pH 6 ~7 during the entire storage period even though it decreased gradually on storage. 2. In general, TBA and VBN of the samples containing herbs were lower than the control. The sample containing 3% onion skin showed the lowest TBA and VBN value. 3. The total plate count of the samples containing herbs was low compared to the control even though the total count increased during the storage. The samples containing 3% garlic and 3% onion skin showed the lowest total plate count on 20 days of storage. 4. The sample with garlic showed remarkably low value in sensory evaluation. The samples containing onion and onion skin, however, reached to the high sensory points as storage period increased. They received high points in taste as well as overall acceptance.

• 한국작물학회지
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• 제46권4호
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• pp.328-333
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• 2001

Allium is one of the largest genera, which has more than 700 species. PCR by URP (universal rice primer) primers was carried out to get phylogenetic information on 26 species, 62 accessions of subgenus Rhizirideum. The accessions were divided into seven groups at 0.76 similarity level. A. tuberosum (Chinese chives) and A. ramosum represented high similarity of 0.91. A. montanum, A. nutans, A. senescens, A. libani, A. odorum, A. austrosibiricum, and A. narcissiflorium grouped at 0.80 similarity. Some of the wild species, such as A. prostratum, A. polyrhizum, A. odorum, and A. mongolicum, showed different band patterns according to polyploidy, occurrence of B-chromosome, collection site, and origin.

• 한국농림기상학회지
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• 제11권4호
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• pp.252-255
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• 2009

한국에 자생하는 Allium속 식물 중에서 개발가치가 있는 산달래, 달래, 야생부추 및 산파를 대상으로 표고 및 지역별 자생지의 특성을 조사하여 육종 및 재배의 기초자료를 얻고자 수행하였다. 산달래와 달래는 모두 300m 이하의 지대에 주로 자생하고, 그 한계는 산달래는 1,000m, 달래는 800m였다. 야생부추는 100m 이하의 저지대에서부터 1,000m 이상의 고지대에 이르기까지 보다 광범위하게 자생하였고, 산파는 900m 이상의 고지대에서만 자생하였다. 산달래가 달래보다 넓고 광범위하게 자생하였고, 자생지의 수도 산달래가 달래에 비하여 많았다. 산달래는 경기도, 강원도, 전라도와 경상도 지역에서, 달래는 충청도와 제주지역에서 비교적 많이 자생하였다. 산달래는 내륙지방이나 해안지방에서도 자생하나, 달래는 해안지방보다는 내륙지역에 주로 자생하였다.

• 한국균학회지
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• 제23권2호통권73호
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• pp.139-143
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• 1995

산마늘에서 분리한 Cladosporium sp.는 Allium속(屬) 식물에서 보고된 C. allii나 C. allii-cepae와는 연쇄상의 다격벽(多隔壁) 분생포자(分生胞子)와 무격벽(無隔壁) 분생자경(分生子梗)을 형성하는 점에서 구별된다. 이 균은 기타 다른 기질(基質)에서 보고된 Cladosporium spp.와 비슷한 점이 없다. 따라서 이 균은 신종(新種)으로 판단되며, Cladosporium alliicola H.D. Shin & U. Braun으로 명명(命名)한다.