• Title/Summary/Keyword: Antibiosis

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Antibiosis of Pediocin-Producing Pediococcus sp. KCA1303-10 Against Listeria monocytogenes in Mixed Cultures

  • Ahn, Cheol;Kim, Chung-Hoi;Shin, Hyun-Kyung;Lee, Young-Min;Lee, Yeon-Sook;Ji, Geun-Eog
    • Journal of Microbiology and Biotechnology
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    • v.13 no.3
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    • pp.429-436
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    • 2003
  • Pediocin K1 is a bacteriocin produced by Pediococcus sp. KCA 1303-10, isolated from traditionally fermented flatfish in Korea. Pediocin K1-dependent antibiosis and pediocin K1-independent antibiosis against Listeria monocyrogenes were investigated by comparing antibiosis potential of the ped+ wild-type strain of Pediococcus sp. KCA1303-10 with that of the ped- mutant strain in 3 different media at 3 different temperatures. In the synthetic MRS-APT medium, bacteriocin (pediocin K1)-dependent antibiosis (BDA) acted as the major driving force of overall antibiosis at the initial stage before the pH of the media was not sufficiently lowered, while bacteriocin-independent antibiosis (BIA) took over the major role at the late stage of antibiosis by killing otherwise resistant cells in the modium. The role of BDA increased as the temperature of the system decreased. The antibiosis potential of BDA among the overall antibiosis of Pediococcus against Listeria at $37^{\circ}C$ was calculated as 46%, and as 75% at $25^{\circ}C$. In the skim milk medium, antibiosis of Pediococcus against Listeria was weakened more than 4 log cycles compared to that of the synthetic medium; however, BDA worked as the main antibiosis force regardless of the culturing temperature in the skim milk medium. In the bean soup medium, BDA also worked as the major killing mechanism against Listeria, but BIA played as another suppressing mechanism against otherwise pediocin-resistant Listeria population. These results suggest that a large portion of the inhibitory action of the ped+Pediococcus sp. KCA1303-10 was attributable to the bacteriocin produced by the strain and that viable Pediococcus sp. KCA1303-10 was superior to the purified bacteriocin in suppressing the occurrence of the bacteriocin-resistant Listeria monocytogenes in food systems.

The Study of Antibiosis of the Fabrics Dyed by Wormwood Extract (쑥 추출액을 이용한 염색직물의 항균성에 관한 연구)

  • 박영희;남윤자;김동현
    • Journal of the Korean Society of Clothing and Textiles
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    • v.24 no.1
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    • pp.67-76
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    • 2000
  • Dyeing fastness and antibiosis effects of fabrics dyed with wormwood, which has been used in folk medicines and dietary life in Korea for a long time, were studied through dyeing small overcoats, which are used as diapers, with wormwood extract. As for the dying fastness test results, the fastness of decoloration was not that good but the pollution level showed a relatively positive result. As for the antibiosis effect results, the antibiosis of the dyed sample was better than the original sample on 3 kinds of bacteria, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis and on 2 kinds of the fungi(Eumycetes). Aspergillus niger and Candida albicans. Especially, the sample that was mordant with CaCl2 and FeSO4·7H2O showed the best antibiosis effect. On the other hand, the sample that showed the best antibiosis effect was the refined fabric, which is a fabric that is not dyed.

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Effect on Plant Growth and Antibiosis of Rice Straw Liquor Extracted from Rice Straw (볏짚에서 추출한 짚초액의 식물성장에 대한 영향과 항균 효과)

  • Kang, Hwa-Young;Kim, Se-Hoon;Kim, Young-Ju;Park, Sang-Sook
    • Journal of Environmental Health Sciences
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    • v.35 no.3
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    • pp.178-186
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    • 2009
  • Rice straw liquid was prepared from rice straw using simple pyrolysis furnace, and its effects on plant growth and antibiosis were investigated. Effects of straw liquid on plant growth of Oryza sativa L., Glycine max Merr. and Lactuca sativa L. and antibiosis of bacteria and mold of rice straw liquid were studied. Th rice straw liquid showed good results on plant growth and multiplication. Rice straw liquid showed a little antibiosis on bacteria but non antibiosis on mold. Rice straw liquid had minimal inhibitory concentration of 2.5-5% for bacteria.

Antibiosis and Bacteriocin Production of Lactic Acid Bacteria Isolated from Kimchi

  • Bae, Sung-Sook;Cheol Ahn
    • Preventive Nutrition and Food Science
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    • v.2 no.2
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    • pp.109-120
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    • 1997
  • In order to elucidate roles of lactic acid bacteria(LAB) for the antibiosis occurring in th fermenting environment of Kimchi, 2.052 strains of LAB were isolated from Kimchi. Fifty tow strains which showed antagonistic effect against 4 indicator strains were finally selected and investigated. Based upon responses to protease treatment, antibiosis of the 52 strains of LAB were classified into 3 types. Type A antibiosis resulted from action of antibiotic-like substances which were not affected by protease treatment and which had broad action spectra against even natural inhabitants of Kimchi. Type B antibiosis was due to bacteriocin-like substances which were very sensitive to treatment of protease and more effective against foreign bacteria than original inhabitant microflora. Type C antibiosis was owing to proteinaceous compounds which were activated or induced by the presence of protease and then exerted antibacterial activities. Therefore, lactic acid bacteria appeared to contribute to antibiosis of Kimchi by the concerted action of these three different types of antibacterial compounds. As one of model system for type B bacteriocin, the antagonistic compound produced by LAB31-9 as well as th producer strain itself was further charaacterized. Strain LAB31-9 was identified as L. casei. Bacteriocin produced by LAB31-9 was proteinaceous and stable over wide range of pH and to various solvents, but very labile to heat treatment. Its mode of action was bactericidal. Based upon these data, bacteriocin produced by LAB31-9 was named as 'caseicin K319'. Genetic determinant for the bacteriocin production of LAB31-9 was located in the chromosome.

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A Study on the Dyeability and Antibiosis of Fabrics Dyed with Solanum Nigrum Extract (까마중 추출물을 이용한 천연 염색직물의 염색성 및 항균성)

  • Park, Young-Hee
    • Journal of the Korean Society of Costume
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    • v.57 no.4 s.113
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    • pp.61-69
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    • 2007
  • The purpose of this study was to identify the utility value with material of natural dyeing and to examine the dyeability and antibiosis of fabrics dyed with Solanum nigrum extract. The results obtained are as follows. First, the color tone of dyed fabrics was tinged with from yellow series to green series, in the chrominance the fabric dyed with Fe mordant was showed the highest value. Second, colorfastness to dyeing showed difference as to mordants, but colorfastness to dyeing except for sunlight colorfastness showed the good colorfastness result on the whole. Third, in the test results of antibiosis, the dyed both cotton and silk fabrics showed the very excellent antibiosis effect for bacteria but showed the insignificant effect for fungus.

A study on the antibiotic effect using the d-limonene oil extracted to wasted mandarin peels in Cheju (제주 감귤피에서 추출한 d-limonene 오일의 항균 효과에 대한 연구)

  • Oh, Eun-Ha;Im, Ho-Sub;Yoon, Cheol-Hun
    • Journal of the Korean Applied Science and Technology
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    • v.26 no.3
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    • pp.350-356
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    • 2009
  • The objection of this research is to conform of practicable possibility and recycling of producing junk after citrus fruits is processed. In the middle of practicable possibility, with verification of antibiosis that tactiling sensibility in a microorganism. With extracting d-limonene oil that have 70${\sim}$90% a component of oil out of junk citrus peel, making certain the about 12000ppm(1.2%) concentration of it. By means of antibiosis property over the a pathogenic bacterium as well as a residence bacterium, considerating the limit of application against daily living supplies needs to antibiosis. Antibiosis effect of a stationing bacterium in the body permanently and by means of antibiosis verification of special bacteria Propionibacterium Acnes that causative skin trouble is selected, in based the antibiotic sencitivity test check up result of minimal inhibitory concentration(MIC).

The Antibiosis of Moisture Water Included the Sophora Radix Extracted by Ethanol Solvent on Bacteria (고삼(苦蔘)(Sopora Radix) 에탄올 추출물(抽出物)을 이용(利用)한 화장수(化粧水)의 항균성(抗菌性))

  • Park, Seon-Yeong;Nam, Yun-Ja;Kim, Dong-Hyun
    • Journal of Fashion Business
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    • v.7 no.5
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    • pp.13-16
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    • 2003
  • The aim of study was to elucidate the antibiosis of the Sophora Radix extracts by ethanol solvent on bacterias. The antibiosis on bacterias was examined in moisture water included the Sophora Radix extract. The results are as follows; Minimum inhibitory concentration (MIC) of Sopora Radix extracts showed markedly lower on gram positive bacterias($0.0125{\sim}0.025$) such as aureus, B. subtilis, S. epidermids than that on gram negative bacterias such as E. coli, P. aeruginosa(>10). The MIC of moisture water included the Sophora Radiix which extracted by ethanol solvent was very low which meaned good antibiosis($0.0063{\sim}0.5$). The antibiosis of moisture water included the Sophora Radiix was improved by adding the ethanol on some baterias. In conclusion, Sophora Radix had excellent antibacterial effect. Therefore, it is expected that Sophora Radix can be practically used as the natural material of functional cosmetics for preventing the Bacterial inhibitory in skin.

The Experimental Study on Antibiosis of Decoctions Made by Cassiae Semen, Celosiae Semen and Buddlejae Flos (결명자(決明子), 청상자(靑葙子), 밀몽화(密蒙花) 전탕액(前湯液)의 항균성에 대한 실험적 연구)

  • Seo, Hyung-Sik
    • Journal of Pharmacopuncture
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    • v.13 no.2
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    • pp.75-83
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    • 2010
  • Objectives : This experimental study was performed to investigate antibiosis of decoctions made by Cassiae Semen(CaS), Celosiae Semen(CeS) and Buddlejae Flos(BF). Methods : Decoctions made by CaS. CeS and BF were prepared. After administering decoctions made by CaS. CeS and BF on bacterial species(Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Aspergillus niger, Fusarium oxysporum, Condida albicans) which cause Keratitis, the size of inhibition zone and MIC(Minimum Inhibition Concentration) were measured Results : The inhibition zone on bacterial species didn't appear, after administering decoctions made by CaS, CeS and BF. Conclusions : This experimental study is showed that decoctions made by CaS, CeS and BF don't have antibiosis on bacterial species which cause Keratitis.

Antibiosis Evaluation of Antimicrobial Mortar by Artificial Accelerating Test for Biochemical Corrosion (생화학적 부식 인공촉진시험에 의한 항균모르타르의 항균성능 평가)

  • Koo, Kyung-Mo;Shin, Kwan-Soo;Roh, Kyung-Min;Lee, Eui-Bae;Kim, Gyu-Yong;Kim, Moo-Han
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2007.11a
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    • pp.33-36
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    • 2007
  • In this study, an antibiosis of antimicrobial mortar developed to reduce biochemical corrosion of sewage facilities concrete was evaluated. First, artificial acceleration test reflecting similarly biochemical corrosion of mortar was developed. Antimicrobial mortar specimen were experimented in this test and antibiosis of it was evaluated by SEM and EDX. As a results of the study, sulfur-oxidizing bacteria on the plain specimen were survived for 20 weeks in this test. But sulfur-oxidizing bacteria on antimicrobial specimen were survived less than the plain, and sulfur-oxidizing bacteria were externally distorted and destroyed. So the antibiosis of an antimicrobial mortar was verified by it.

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