• Title/Summary/Keyword: Anticancer activity

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Anticancer Effect of Hydrophobic Protein Fraction from Red Ginseng by Clonogenic Assay (Clonogenic Assay에 의한 홍삼 소수성단백질 분획의 항암효과)

  • Kim, Chang-Han;Lee, Myung-Sub;Lee, Kyung-Ho
    • Journal of Ginseng Research
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    • v.19 no.1
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    • pp.27-30
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    • 1995
  • We established the model of clonogenic assay with cancer cell lines such as SW-156(kidney), SNU-5(stomach), Hep G2(liver), and WiDr(colon), and we investigated anticancer effect of hydrophobic protein fraction(N-fraction) from Korea red ginseng by using this model. The results of clonogenic assay showed that N-fraction had anticancer activity against SNU-5 above 100 $0.2\mu\textrm{g}$/ml concentration, and did not exhibit anticancer activity against cell lines such as SW-156, WiDr, and Hep G2 up to 1,000 $\mu\textrm{g}$/ml concentration. This result suggests that N-fraction has specially anti-stomach cancerous effect.

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Synthesis and Biological Evaluation of Militarin Derivatives (밀리타린 유도체의 합성 및 생리활성 평가)

  • Che, Haiyan;Yoon, Deok Hyo;Kim, Tae Woong;Sung, Gi-Ho;Park, Haeil
    • YAKHAK HOEJI
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    • v.56 no.6
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    • pp.372-376
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    • 2012
  • Militarin was isolated from Cordyceps militaris, a caterpillar-grown traditional medicinal mushroom and reported to possess anticancer activities against several tumor cells. A number of militarin derivatives bearing different numbers of ethylene glycol unit were synthesized and evaluated for their biological activities related with anticancer and antiinflammatory effects. Among militarin derivatives tested, the compounds 2 and 5 exhibited anticancer and antiinflammatory activities.

Anticancer Effects of Natural Medicinal Plant Extracts on Oral Carcinoma Cells (천연 약용식물 추출물의 구강상피세포암 세포주에 대한 항암효과)

  • 김정희;현진원;김여갑
    • Biomolecules & Therapeutics
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    • v.7 no.2
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    • pp.153-157
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    • 1999
  • The anticancer effect of medicinal plants against two oral carcinoma cells, A253 and SCC-25 were investigated in this study. Methanol extracts from 63 medicinal plants, which have anticancer activities against other cancers such as stomach, hepatocellular or colon carcinomas, were prepared and screened for their anti- oral cancer activity by using MTT assay. Thirty one samples showed anti-oral cancer activity against either cell line used, however, other 32 samples had no anti-oral cancer activity. Among these samples methanol extract of Caesalpinia sappan revealed the strongest anti-oral cancer activity. The $IC_{50}$/ values of this extract against A253 and SCC-25 cells were 16 and 25 $\mu$g/m1, respectively. Fractions of n-hexane, dichloromethane, ethylacetate, n-buthanol and water were prepared from methanol extracts of Caesalpinia sappan, Anthriscus sylvestris, Rhus japonica, Curcuma arowatica, Inula helenium, Sinoarnudinaria reticulata, and Polygonum cuspidatum, respectively. Among these 35 fractions the n-hexane fraction of Inula helenium showed the strongest anti-oral cancer activity, the $IC_{50}$/ value was 1.6$\pm$0.3 $\mu\textrm{g}$/ml. Ten other fractions showed $IC_{50}$/ values lower than 10 $\mu\textrm{g}$/ml.

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Isolation of the ppar-${\gamma}$ ligands from the stem of the Zanthoxylum Schinifolium and their structure activity relationships

  • Nam, Jeong-Bum;Lee, Jeong-Hyung;Kim, Young-Ho;Lee, Jung-Joon
    • Proceedings of the PSK Conference
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    • 2002.10a
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    • pp.380.2-380.2
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    • 2002
  • Peroxisome proliferator-activated receptor (PPAR)-$\gamma$ is a nuclear hormone receptor family that plays an important role in the transcriptional regulation of genes in cellular lipid and energy metabolism. In our search for Iigands for PPAR-$\gamma$ from natural resources. two phenylpropanoids. 3.4.5-Trimethoxy cinnamylalcohol (1) and 3.4.5- Trimethoxy cinnamaldehyde (2). were isolated as PPAR-$\gamma$ agonists from the MeOH extracts of Zanthoxylum schinifolium Sieb. & ZUCCo (Rutaceae) by activity-guided fractionation. These two compoundS bind and activated PPAR-$\gamma$ transcriptional activity in a dose dependent manner assessed by ligand-binding assay. While the maximum activities for PPAR-$\gamma$ of these compounds were comparable with that of rosiglitazone. which is currently used in the treatment of Type II diabetes. the potency of these compounds were much weaker than rosiglitazone ($ED_{50}$=t.2$\mu\textrm{M}$) with the $ED_{50}$ values of 9.08 and 4.08 $\mu\textrm{M}$. respectively. To examine the structure-activity relationship of phenylpropanoids. we prepared several phenylpropanoid derivatives and measured the activity. We observed that substituents at 4'- position could playa key role in determining the potency for PPAR-$\gamma$ agonistic activity .

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The Role of Substituents of ar-Turmerone for its Anticancer Activity

  • Oh, Won-Geun;Baik, Kyong-Up;Jung, Sang-Hun;Ahn, Byung-Zun
    • Archives of Pharmacal Research
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    • v.15 no.3
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    • pp.256-262
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    • 1992
  • For the evaluation of the role of substituents of ar-turmerone for its anticancer activity, ar-turmerone (1a) and its analogs like 2-methyl-6-(4'-methyphenyl)-2-octen-4-one (1b), 2-methyl-6-phenyl-2-hepten-4-one (1c), 2-methyl-6-phenyl-2-octen-4-one (1d) and 2 methyl-6-(trans-4'-methylcyclohexyl)-2-hepten-4-one (1e) were preparedd and their cytotoxic activities against $L_{1210}$ cell were determined. Omission of methyl group at para-position dose not variate the cytotoxicity of ar-turmerone. Elongation of alkyl group at 6-position decreases $ED_{50}$ value. Saturation of aromatic ring of ar-turmerone markedly decreases the cytotoxicity. Therefore the smaller size of alkyl group at 6-position and aromatic ring of ar-turmerone should be essential for exhibiting its anticancer activity.

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Characteristic Features of Cytotoxic Activity of Flavonoids on Human Cervical Cancer Cells

  • Sak, Katrin
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.19
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    • pp.8007-8018
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    • 2014
  • Cervical cancer is the most common gynecologic malignancy worldwide and development of new therapeutic strategies and anticancer agents is an urgent priority. Plants have remained an important source in the search for novel cytotoxic compounds and several polyphenolic flavonoids possess antitumor properties. In this review article, data about potential anticarcinogenic activity of common natural flavonoids on various human cervical cancer cell lines are compiled and analyzed showing perspectives for the use of these secondary metabolites in the treatment of cervical carcinoma as well as in the development of novel chemotherapeutic drugs. Such anticancer effects of flavonoids seem to differentially depend on the cellular type and origin of cervical carcinoma creating possibilities for specific targeting in the future. Besides the cytotoxic activity per se, several flavonoids can also contribute to the increase in efficacy of conventional therapies rendering tumor cells more sensitive to standard chemotherapeutics and irradiation. Although the current knowledge is still rather scarce and further studies are certainly needed, it is clear that natural flavonoids may have a great potential to benefit cervical cancer patients.

Synthesis of New 4-(tert-Octyl)phenol Derivatives and Their Anticancer Activity against Human Prostate and Lung Cancer Cell Lines

  • Che, Haiyan;Fang, Yuanying;Gurung, Santosh K.;Luo, Jun;Yoon, Deok Hyo;Sung, Gi-Ho;Kim, Tae Woong;Park, Haeil
    • Bulletin of the Korean Chemical Society
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    • v.35 no.7
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    • pp.2038-2042
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    • 2014
  • 4-(tert-Octyl)phenol derivatives bearing the D-mannitol substructure (6a, 6b, 7) were prepared from $\small{D}$-mannitol and evaluated their anticancer activity against human lung (A549) and prostate (Lncap, Du145, PC3) cancer cell lines. Among derivatives tested, the bis(tert-octyl)phenoxy compound 7 exhibited strongest proliferation inhibitory activities against human cancer cell lines tested, especially high sensitivity to human Du145 prostate cancer cells ($IC_{50}=7.3{\mu}M$).

Protective Effects of Vitamin C on Cisplatin Naphrotoxicity

  • Choung, Se-Young;Kong, Jae-Myeong
    • Archives of Pharmacal Research
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    • v.17 no.1
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    • pp.11-16
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    • 1994
  • Cis-dichlorodiammineplatinum(II)(cisplatin) is one of the most effective antitumor agents currently available for cancer therapy. However, its clinical use has been limited by its severe side effects, especially nephrotoxicity. To evaluate the effect or radical scavengers on cisplatin nephrotoxicity in rats, cisplatin and Vitamin C were given intraperitoneally. Remarkable protective effects of Vitamin C against nephrotoxicity of cisplatin were observed when Vitamin C was administered to rats 1hr before cisplatin injection. hepatotoxicity induced by combination treament of cisplatin and Vitamin C was evaluated by measuring serum glutamic pyruvate transmainase(sGPT) and serum glutamic oxalate transminase(sGOT). Combination treatment did not affect the levels of sGPT and sGOT, and any combination treatment did not induce metallothionein biosynthesis in kidny, Vitamin C which has radical scavenging effect induce metallothionein biosynthesis in kidney. Vitamin C which has radical scavenging effect directly reduced nephrotoxicity of cisplatin in vivo. Thus, it seems that free radical is the cause of cisplatin nepthrotoxicity. Also, combination treatment did not reduce anticancer activity of cisplatin. The present results indicate that Vitamin C, when it is given with cisplatin, may provide protection against cisplatin nephrotoxicity without reducing anticancer activity.

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Synthesis of Polyamides Containing N-Methylpyrrole and N-Methylimidazole and Their Anticancer Activity

  • Yuan, Gu;Xiao, Junhua;Huang, Weiqiang;Tang, Feili;Zhou, Yawei
    • Archives of Pharmacal Research
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    • v.25 no.5
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    • pp.585-589
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    • 2002
  • Three hairpin polyamides were designed and synthesized by a haloform reaction and DCC/HOBt coupling reaction without amino protection and deprotection. Their anticancer activity were investigated with three kinds of cell lines-hepatic carcinoma, lung carcinoma and gastric carcinoma, and the values of $IC_{50}$ were at range of $10^{-7}~10^{-8}M$.

Physiological Activities of Opuntia humifusa Petal (천년초 꽃잎의 생리활성)

  • Jung, Bok-Mi;Shin, Mi-Ok
    • Korean journal of food and cookery science
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    • v.27 no.5
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    • pp.523-530
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    • 2011
  • This study was conducted to investigate antimicrobial, antioxidant and anticancer activities of Opuntia humifusa (OH) petal extracts. The methanol and hexane extracts of OH petals showed their highest antimicrobial activity against Clostridium perfringens. The OH petal butanol fraction had the best antioxidative peroxynitrite scavenging activity among OH petal extracts. The DPPH scavenging activity of OH petals was lower than the peroxynitrite scavenging effect. The hexane and methanol fractions at a concentration of 200 ${\mu}g$/mL inhibited proliferation >80% in four kinds of human cervical cancer cells(B16F10, HepG2, HT-29 and MCF-7). In particular, the anticancer effect against B16F10 human skin cancer cells at the same concentration was higher than that in the other cancer cells.