• Preventive Nutrition and Food Science
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• 제11권4호
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• pp.278-284
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• 2006

The beneficial effects of digested onion extracts have been assessed by antimutagenic and anticancer activities by Ames test and SRB test. The total phenolic acids and flavonoids in onion extracts were determined. Red and yellow onions contain more phenolic acids and flavonoids than those in the white onion. Digested, extracts showed antimutagenic activity and anticancer activity, and it appears that the antimutagenic activity of digested extracts of onion against mutagens and anticancer activities were related to their phenols and flavonoids contents. Moreover, the extracts inhibited the proliferation of four human tumorigenic cell lines such as HT-29 (colon), MCF-7 (breast), DU-145 (prostate) and HepG2 (liver), in a dose-dependent manner. Phenolic acids and flavonoids caused oxidative damage to the cancer cell lines and induced apoptosis. Generally, red onion extracts showed effective antimutagenic and anticancer activity, and the digested red onion extracts elicited stronger antimutagenic activity than those of the onion extracts without digestion.

• 한국미생물·생명공학회지
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• 제27권1호
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• pp.15-22
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• 1999

Various lactic acid bacteria were isolated from Korean Kimchi in order to study their antimutagenic substances. Ames test using Salmonella typhimurium TA98 and TA100 showed the strain KLAB21 to have the highest antimutagenic activity among the 230 isolated strains against MNNG (N-methyl-N'-nitro-N-nitrosoguanidine), NPD (4-nitro-O-phenylenediamine), NQO (4-nitrosoquinoline-1-oxide) and AFB1 (aflatoxin B1). The strain was identified as Lactobacillus plantarum based on its morphological, cultural and physiological characteristics. Antimutagenic activity of L. plantarum KLAB21 was found in culture supernatant suggesting the bacterium secrete antimutagenic substance in the media. No mutagenic activity was found in the culture supernatant. The isolated strain L. plantarum KLAB21 showed much higher antimutagenic activity than L. plantarum IAM1261 which is being used industrially for fermented milk production. The antimutagenic activity of L.plantarum KLAB21 was reconfirmed by the spore-rec assay using spores of Bacillus subtilis H17($Rec^+$) and M45($Rec^-$).

• 한국지역사회생활과학회지
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• 제15권1호
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• pp.49-55
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• 2004

The purpose of this study is to research the antimutagenic and antioxidative effects of water dropwort and small water dropwort by Ames test and by measuring malondialdehyde(MDA) production. Water dropwort and small water dropwort were extracted with methanol and then further fractionated to hexane, chloroform, ethyl acetate, butanol and water, stepwise. The methanol extracts from both samples reduced the mutagenicities by aflatoxin $B_1(AFB_1)$ in Salmonella typhimurium TA 98. The production of MDA also decreased when the methanol extracts were added to the system. The hexane, chloroform and ethyl acetate fractions revealed higher antimutagenic activities against $AFB_1$ than the butanol and water fractions. Among the five fractions, the ethyl acetate fraction showed the highest level of antioxidant activity. From the results of the experiment, water dropwort and small water dropwort seem to be good antimutagenic and antioxidative sources of food.

• Preventive Nutrition and Food Science
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• 제2권2호
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• pp.155-161
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• 1997

The mutagenic and antimutagenic activities of juices from 20 common edible wild herbs found in Korea were investigated using the spore-rec assay and Ames test. The juices of Hemerocallis fulva and Capsella bursapastoris exhibited a little induction or inhibition of mutagenesis in the presence of selected metal ions, but juices of most edible wild herbs did not affect on the mutagenesis in the spore-rec assay. In the other hand, all of the juices strongly inhibited the mutagenesis induced by benzo[a]pyrene, 2-amino-fluorene, and 3-amino-1,4-dimethyl-5H-pyridol tested on Salmonella typhimurium TA98 or TA100 in the presence of S-9 mix. The antimutagenic effects increased as the concentration of the mutagens increase. The results suggest that concentration of samples or types of various mutagen interact to affect the antimutagenic potential of the juices in the TA98 and TA100 strain.

• 한국식품위생안전성학회지
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• 제10권3호
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• pp.133-138
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• 1995

In vitro antimutagenic activity of methanol extract from brrwn rice and its physico-chemical characteristics were investigated using Salmonella typhimurium reversion assay and SOS chromotest. Methanol extracts of brown rice were not mutagenic compared with direct and indirect, mutagenicities of 4NQO (4-nitroquinoline oxide), 2NF(2-nitrofluorene), Trp-p-1(3-Amino-1,4-dimethyl-5H-pyrido-[4,3-b]indole), and Trp-p-2(3-Amino-1-methy-5H-pyrido-[4,3-b]indole). Antimutagenic activity against the indirect mutagenicties induced by Trp-p-1, Trp-p-2 and AFB1 (aflatoxin B1) was found in methanol extract. Even though antimutagenic activity showed dose-dependent, it remained constant at inhibition rate ranging 60~90% when the concentration was abov 3mg/plate in the S. typhimurium reversion assay and 0.2~0.6 mg/assay in the SOS chromotest. The antimutagenic activity of the methanol extracts was stable at various pH (2, 7 and 10), temperatures (60, 80 and 10$0^{\circ}C$)and heation times (2, 4, 6, 8, 10 min at 10$0^{\circ}C$).

• Applied Biological Chemistry
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• 제39권6호
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• pp.419-423
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• 1996

SOS chromotest 방법을 이용하여 천연물에 포함된 항변이원성 물질의 활성을 정량화 할 수 있는 항변이원 활성단위 측정법을 개발하였다. 이 방법에 의하여 시료의 항변이원 활성을 특정 화학적 변이원이 유도하는 변이원성이 표준측정 조건하에서 50% 억제되는 시료의 양으로 수치화하는 것이 가능하게 되었다. 확립된 방법으로 쌀의 품종별 항변이원 활성 및 기타 곡류의 항변이원 활성을 조사하였다. 그 결과 변이품종 쌀인 자도와 향도가 취반용으로 사용되는 추청보다 항변이원 활성이 높았고, 일반적으로 검정콩이나 팥과 같은 잡곡의 항변이원 활성이 현미보다 높았다.

• 생명과학회지
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• 제5권1호
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• pp.26-32
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• 1995

The antimutagenic effects of insoluble dietary fibers(IDF) extracted from some green-yellow vegetables(kale, carrot, spinach, broccoli and soybean sprout) and soybean by binding the carcinogens of MeIQ (2-amino-3,4- dimethyl-imidazo(4,5-f) quinoline) and Trp-P-2(3-amino-1-methyl-5H-pyrido[4,3-b] indole) in Salmonella tylhimirium TA100 and TA98 were studied. All of the insoluble dietary fiber samples which binded MeIQ exhibited high antimutagenic effects by removing the mutagen. Among the samples, IDFs from kale and soybean showed strong binding capacity fo the carcinogen and revealed about 90% of the antimutagenic activity. the IDF samples showed somewhat lower binding capacity to the Trp-p-2. The lignin which extracted from kale, soybean and carrot, and the cellulose strongly removed the mutagenicity of MeIQ by the binding. Among the samples, the level of lignin in kale revealed the highest(about 10%), and it seemed that the higher content of lignin in kale is one of the reasons to increase its antimutagenic effect.

• 생명과학회지
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• 제11권5호
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• pp.432-438
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• 2001

우리나라 동치미 발효에 관여하는 유산균의 항돌연변이활성을 조사하기 위하여 숙성중의 동치미부터 300여주의 유산균을 분리하였다. 이들 균주들의 항돌연변이활성이 활성을 Salmonella enterotica serovar typhimurium TA100과 TA98을 이용하여 조사하고 직접 변이원 MNNG, NPD, 4-NQO와 간접변이원으로서 AFB1에 대하여 항돌연변이 활성이 가장 우수한 DLAB19균주를 최종 선별하였다. 최종 선별한 DL-AB19의 형태학적, 배양학적 및 생리학적 특성을 조사하여 Bergeys Manual Systematic Bacteriology의 분류 기준에 따라 동정한 결과 Leuconostoc mesenteroides subsp. cremoris 또는 그 유연균으로 동정하였다. Leu. mesenteroides subsp. cremoris DLAB19의 배양 상징액의 MNNG, NPD, 4-NQO 및 AFB1에 대한 항돌연변이 활성은 100$\mu$ι/plate 첨가시활성이 가장 높게 나타났다. 또한 B. subtilis의 포자를 이용한 spore-assay 방법을 사용하여 MNNG와 4-NQO에 대한 항돌연변이 활성을 가지고 있음을 재확인하였다.

• 한국식품저장유통학회지
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• 제5권4호
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• pp.386-391
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• 1998

김치로부터 분리 동정된 Lactobacillus plantarum KLAB21 균주에 있어서 항돌연변이 활성물질 생산을 위한 최적 조건을 조사하였다. 탄소원으로서 glucose 첨가시 가장 높은 항돌연변이 활성을 나타내었으며, 질소원으로서는 yeast extract와 bactopeptone 첨가시 활성이 우수하였다. 탄소원인 glucose의 농도는 2%에서, 질소원인 yeast extract와 bactopeptone의 농도는 1%에서 가장 우수한 항돌연변이 활성을 나타내었다. 항돌연변이 활성의 최적 배양 조건은 초기 pH 배양온도, 배양속도 각각 7.0, 37$^{\circ}C$, 150rpm이었다. 상기의 최적 조건에서 36시간 배양시 가장 높은 항돌연변이 활성을 나타내었는데 S. typhimurium TA100과 S. typhimurium TA98을 이용한 조사결과 항돌연변이 활성이 각각 73.95%, 59.47%이었다.

• 한국식품과학회지
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• 제30권3호
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• pp.688-692
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• 1998

천연생리활성물질의 개발과 미이용자원의 효율적 재이용방안을 모색하기 위하여 13여종의 한국산 야생 버섯류를 에탄올과 물로 추출하여 각 시료추출물의 항변이원성 물질 검색을 시도하였다. B(a)P과 MNNG의 돌연변이 유발성에 미치는 야생버섯류의 에탄올 추출물과 물추출물의 항돌연변이 효과의 검토는 Salmonella typhimurium TA98과 TA100을 이용한 Ames 실험계를 이용하였으며 B(a)P에 대해 꽃구멍장이버섯(Polyporus dispansus), 깔때기꾀꼬리버섯(Cantharellus infundibuliformis), 진갈색주름버섯(Agaricus subrutilescens), 테미로버섯(Daedalea dickinsii), 목장말똥버섯 (Panaeolus papilionaceus) 및 턱수염버섯(Hydnum repandum) 등의 에탄올추출물에서 비교적 강한 항변이 원성을 나타내었다. 물추출물의 경우는 B(a)P에 대해서 턱수염버섯(Hydnum repandum)이 TA100에서 가장 강한 저해효과를 나타내었고 대부분의 시료는 낮거나 저해활성이 보이지 않았다. 직접변이원인 MNNG에 대해서는 야생버섯류의 에탄올추출물중 5종만 항변이원성이 관찰되었고, 물추출물의 경우는 꽃구멍장이버섯(Polyporus dispansus)이 가장 강한 저해효과를 보였으나 대부분의 버섯류는 낮거나 저해 효과를 거의 나타내지 않았다. 그중 직, 간접 변이원에 대해 가장 강한 저해 효과를 나타낸 꽃구멍장이 버섯의 에탄올추출물은 농도가 높을수록 항변이원성 효과가 증가하는 경향을 보였다.