• 한국어병학회지
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• 제35권1호
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• pp.41-46
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• 2022

Since viral haemorrhagic septicaemia virus (VHSV) was first reported in European rainbow trout (Oncorhynchus mykiss) in the 1930s, it has caused high prices in freshwater and saltwater fish around the world, causing enormous economic damage to the aquaculture industry. We have been seeking required countermeasures against viruses because of economic damage to the aquaculture industry. However, commercial vaccines have the limitations of being costly to use in farms and being effective to only one pathogen. The aquaculture industry these days is taking on new alternatives to vaccines, antibiotics and chemicals. In this study, the suitability of antiviral effects against VHSV was evaluated in vitro for various plant extracts to judge their effectiveness. Atriplex gmelinii, Ixeris repens, Arctium lappa, and Sargassum coreanum were tested to know the correlation between the amount of virus and the concentration of extract investigates if these extracts have antiviral effects. Virus and extracts at various concentrations were inoculated simultaneously as 1:1 ratio into EPC cell lines. There are no antiviral effects with Atriplex gmelinii, Ixeris repens and Arctium lappa. Extract of Sargassum coreanum only has the antiviral activity in a dose-dependent manner. These results show that extract of Sargassum coreanum can be used in aquaculture industry as an antiviral materials.

• 식물분류학회지
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• 제51권4호
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• pp.391-394
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• 2021

Arctium tomentosum Mill. (Asteraceae), from Ulaanbaatar in the Khentei phytogeographical region of Mongolia, is recognized here as a new genus and species of the flora of Mongolia. Arctium differs from other genera of Asteraceae by the hooked apex of the involucral bracts. While A. tomentosum is most similar to A. lappa, it is easily distinguished by the glandular hairs of the corolla limb and the widened inner involucral bracts. Taxonomic notes, a description of the morphology, detailed photos, habitat information, the phenology and a distribution map of A. tomentosum are provided.

• 생명과학회지
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• 제22권8호
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• pp.1092-1098
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• 2012

본 연구에서는 우방자를 70% 에탄올로 추출하여 얻어진 추출물을 n-hexane, methylene chloride, ethyl acetate로 순차용매 분획하였다. 각 분획물에 대해 activity-guided isolation을 수행하여 활성물질의 분리 정제를 실시하였다. 활성을 나타내는 물질은 silica gel chromatography (230 mesh), sephadex LH 20, recrystallization method를 이용하여 분리하였다. 각 화합물의 화학구조는 NMR 스펙트럼 데이터 해석하였고 arctiin, arctigenin, diarctigenin, matairesinol 으로 동정하였다. 이들을 human dermal fibroblast HS68 세포에 처리하여 얻어진 상등액은 ELISA kit를 활용하여 procollagen type I 생합성과 MMP-1 저해활성을 측정하였다. 측정결과 procollagen type I 생합성과 MMP-1 저해활성 결과 모두 arctiin이 가장 우수한 결과를 보였다. 이와 같은 결과를 통해 우방자에서 분리한 리그난 화합물들을 이용하여 주름개선 소재로 개발할 수 있을 것으로 사료 된다.

• 생명과학회지
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• 제29권12호
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• pp.1358-1363
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• 2019

본 연구에서는 우엉의 뿌리를 차의 원료로 가공하는 과정에서 필요한 적정한 가열 처리 조건 설정에 대한 자료를 얻고자 열처리 단계의 가열 온도와 시간에 따른 유리아미노산의 함량 변화와 항산화 활성수준 등을 비교하였다. 항산화 활성을 비교하기 위해 실시한 DPPH와 ABTS 라디칼 소거능 측정 결과에서 180℃-4분 가열 처리 조건과 200℃-3분 가열 처리 조건이 비교적 높은 활성을 가지는 것으로 나타났다. 이는 폴리페놀 함량 측정에서 200℃-3분 가열 처리 조건과 180℃-4분 가열 처리 조건에서 각각 62.74 mg/l와 60.09 mg/l로 다른 가열 조건보다 상대적으로 높은 결과를 보인 것과 관련된 것으로 판단되었다. 가열 조건별 우엉차의 유리 아미노산 분석 결과에서 가열온도와 시간이 증가됨에 따라 초기의 아미노산 함량보다 감소되는 경향을 확인할 수 있었다. 특히 phenylalanine, histidine, leucine과 isoleucine 등의 함량 변화가 높은 비율로 나타났으며, aspartic acid, proline 등은 비교적 감소 비율이 낮았다. 전반적으로 180℃에서 3분~4분 가열 조건과 200℃의 2분 가열 조건을 넘어서는 온도와 시간 조건에서는 아미노산 함량이 급격히 감소되었다. 이상의 결과에서 실험이 실시된 항산화 활성과 유리 아미노산 함량을 기준으로 180℃-4분 수준의 가열 처리 조건이 가장 우수한 것으로 판단되었다.

• Archives of Pharmacal Research
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• 제18권6호
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• pp.462-463
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• 1995

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2007년도 추계학술발표회
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• pp.363.2-363.2
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• 2007

• 한국자원식물학회지
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• 제21권6호
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• pp.413-419
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• 2008

Phenolics level, total flavonoid content, and antioxidant were determined from the methanol extracts of the eight medicinal plants using roots. Total phenolics were found as the highest levels in the methanol extracts of Arctium lappa, and followed by Youngia sonchifolia and Cirsium japonicum. Total amount of the each phenol compounds were detected in C. japonicum extracts ($319.2\;mg\;kg^{-1}$) as the greatest component, and followed by A. lappa ($96.3\;mg\;kg^{-1}$) and Y. sonchifolia ($22.9\;mg\;kg^{-1}$). Total flavonoid content showed the highest amount in methanol extracts from A. lappa (68.1 mg $100\;g^{-1}$) and followed by Y. sonchifolia (11.2 mg $100\;g^{-1}$) Lycoris radiate extracts (87.2%) had the highest nitrite scavenging activity and followed by A. lappa (81.5%) and Y. sonchifolia (77.5%). Methanol extracts of A. lappa at 25 mg $100\;g^{-1}$ exhibited the highest DPPH radical scavenging activity by 90.8%, even though less activity than synthetic antioxidants Vitamin C or butylated hydroxytoluene (BHT). Level of polyphenols was highly correlated with anti oxidative activity ($r^2$ = 0.85). The results suggest that several medicinal plants selected had the potent biological activities, and that their activities were differential depending on plant species.

• 한국약용작물학회지
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• 제24권5호
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• pp.351-359
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• 2016

Background: We investigated the optimal aqueous extraction conditions for recovery of high yields of total phenolic compounds from roots of Arctium lappa L. (burdock, Asteraceae), and we compared their antioxidant capacity. Methods and Results: The antioxidant activity of the extracts was tested using 2,2-diphenyl-1-picrylhydrazyl, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)-diammonium salt, and oxygen radical absorbance capacity assays. In addition, the major phenolic compounds present in the extracts were determined by high performance liquid chromatography analysis. Our results suggest that the roasted burdock $100^{\circ}C$, 15 min extract exhibited the strongest radical scavenging activity and possessed the highest concentration of phenolic compounds. The polyphenol content of both dried burdock and roasted burdock significantly increased with increase in the extraction temperature and time. Conclusions: These results indicated a relationship between phenolic compound levels in burdock and their free radical scavenging activities. This suggests that phenolic compounds significantly increase the antioxidant potential of burdock extracts.

• 한국식품조리과학회지
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• 제33권3호
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• pp.325-332
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• 2017

Purpose: The study investigated the quality characteristics of cookies prepared 0, 10, 20 and 30% burdock (Arctium lappa L.) powder. Methods: Cookies were manufactured with 0%, 10%, 20%, and 30% wheat flour substituted for burdock powder. Results: Density of the dough showed no significant differences with increasing amount of burdock powder, whereas pH of the dough decreased with increasing burdock powder content. The pH of cookies decreased with increasing burdock powder content, whereas $^{\circ}brix$ of cookies increased with burdock powder content. The moisture contents of cookies increased with increasing burdock powder content. The spreadability of cookies decreased with increasing burdock powder content. The L-value of cookies decreased, a-value increased, and b-value decreased with increasing burdock powder content. The hardness of cookies decreased with increasing burdock powder content. Total polyphenol contents and DPPH radical scavenging activity increased with increasing burdock powder content (p<0.05). In the sensory evaluation, appearance, color, flavor, and texture were highest in the control group, whereas sweet taste and savory taste were highest in the added group. For overall acceptance of cookies, 10% burdock powder cookies showed higher values than the control and other samples. Conclusion: Results suggest that cookies manufactured with 10% burdock powder are the most optimal product.

• 한국자원식물학회지
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• 제25권1호
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• pp.1-6
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• 2012

본 연구는 우엉추출물의 항염증효과를 측정하였다. 인간 유래 폐상피세포 A549를 이용하여 염증반응을 유발하고 유지시키는데 중요한 역할을 나타낸다고 알려진 ICAM-1의 억제적 조절 효과를 평가하였으며, 염증매개인자로 알려진 NO와 이의 생성에 영향을 미치는 iNOS의 발현조절에서도 억제효과가 있음을 확인하였다. 또한, 우엉추출물이 인간각질형성세포주의 세포증식에 효과가 있음을 나타내었다. 본 연구에서 사용되는 폐상피세포는 호흡기질환의 천식 등의 알러지 및 만성염증연구에 주로 활용되고 있으며, 각질형성세포는 아토피 피부염의 만성 염증반응에서 주요하게 확인하는 요소이다. 따라서 본 연구결과에서 나타난 우엉추출물의 항염증 효과는 다양한 염증반응에서도 특히, 알러지 및 아토피 피부염 질환을 더욱 악화시키는 만성 염증에 있어서 우엉추출물이 유용한 소재로 사용될 수 있음을 시사한다고 할 수 있다.