• The Plant Pathology Journal
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• v.35 no.5
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• pp.406-416
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• 2019

Strawberry, an important fruit crop, is susceptible to a large number of pathogens that reduce fruit quality and productivity. In this study, the effect of a biostimulant prepared from Ascophyllum nodosum extract (ANE) (0.1%, 0.2%, and 0.3%) was evaluated on powdery mildew progression under greenhouse and field conditions. In the greenhouse, application of 0.2% ANE showed maximum reduction in powdery mildew progression as compared to the control. Forty-eight hour post-inoculation, foliar spray of 0.2% ANE reduced spore germination by 75%. Strawberry leaves sprayed with ANE showed higher total phenolic and flavonoid content in response to powdery mildew infection. Furthermore, application of ANE elicited defense response in strawberry plants by induction of defense-related enzymes, such as phenylalanine ammonia lyase, polyphenol oxidase, and peroxidase activity. In field conditions, foliar spray of 0.2% ANE showed a reduction of 37.2% of natural incidence of powdery mildew infection as compared to the control. ANE sprayed plant also reduces the severity of powdery mildew infection under natural conditions. These results indicate that application of ANE induces the strawberry plant's active defense against powdery mildew infection by induction of secondary metabolism and regulating the activities of defense-related enzymes.

• Journal of the Korea Organic Resources Recycling Association
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• v.10 no.4
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• pp.81-85
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• 2002

This study was carried out to investigate the effect of organic fertilizer(OM-1 : $300kgha^{-1}$, OM-2 : $600kgha^{-1}$), microorganism(ML : liquid form, MP : powder form) and seaweed extract ; ascophyllum nodosum(AF : foliar application, AI ; irrigation) on the growth and yield of the chinese cabbage. At the treatment of organic fertilizer OM-2 have appeared the best yield. According to the application of microorganism appeared certainly increasing yield of cabbage by application of powder form. The excess application of organic fertilizer OM-2 should not be occurred fertilizer damages and increased on the growth and yield by use of microorganism, In application of sewweed extract treatment, the cabbage yield increased by irrigation method.

• ALGAE
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• v.29 no.3
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• pp.237-247
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• 2014

Polysaccharides prepared from marine algae sometimes contain contaminants such as polyphenols and endotoxins that may mislead their bona fide biological activities. In this study, we examined bioactive contaminants in commercially available fucoindan from Fucus vesiculosus, along with ascophyllan and fucoidan from Ascophyllum nodosum. F. vesiculosus fucoidan inhibited the growth of Vibrio alginolyticus in a concentration-dependent manner ($0-1,000{\mu}g\;mL^{-1}$). However, the antibacterial activity of the fucoidan significantly reduced after methanol-extraction, and the methanol-extract showed a potent antibacterial activity. The extract also showed cytotoxicity to RAW264.7 and U937 cells, and induced apoptotic nuclear morphological changes in U937 cells. These results suggest that the antibacterial activity of the fucoidan is partly due to the methanol-extractable contaminants that can also contribute to the cytotoxicity on RAW264.7 and U937 cells. On the other hand, the activities to induce secretion of nitric oxide and tumor necrosis factor-${\alpha}$ from RAW264.7 cells were observed in the fucoidan even after methanol extraction, and the extract had no such activities. Our observations suggest that commercially available fucoidan should be purified prior to biochemical use.