• Korean Journal of Veterinary Research
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• v.18 no.1
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• pp.27-31
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• 1978

In order to identify unknown Babesia spp. which was isolated from Korean cattle, the morphology of Korean strain was compared with that of Babesia bigemina (Kochinda strain) and Babesia spp. (Miyake strain). Immunofluorescent technique was used to identify the serological character of the parasites. The results obtained were summarized as follows: 1. Korean strain was morphologically very similar to Babesia spp.(Miyake strain) which mostly showed parallel-bigeminate forms, while B. bigemina (Kochinda strain) was mostly round and oval forms. 2. By the indirect fluorescent antibody technique: a) Anti-Babesia spp. and Korean Babesia spp. sera showed a higher antibody titers with Babesia spp. (Miyake strain) antigen (1:500) than with B. bigemina (Kochinda strain) antigen (1:50). b) Anti-Babesia bigemina sera showed a lower titer with Babesia spp. antigen (1:50) than with B. bigemina antigen (1:250). 3. On the basis of morphological and serological confirmantions, a Babesia strain isolated from a Korean cattle was very similar, if not identical, to Miyake strain of Babesia spp.

• Korean Journal of Veterinary Service
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• v.32 no.4
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• pp.377-380
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• 2009

Dogs with canine babesiosis may present with wide variation in the severity of clinical signs, ranging from a hyperacute, shock-associated, hemolytic crisis to an inapparent, subclinical infection. Dogs typically present with the acute form of babesiosis, which is characterized by general findings such as pyrexia, weakness, mucous membrane pallor, depression, hemorrhagic anemia. This study was conducted to investigate the prevalence of babesia spp. infection in dogs of Seogwipo-si. A survey of canine babesia spp. infections among 173 dogs in Seogwipo-si was performed from July 2008 to August 2008. Blood samples were collected from dogs raised outdoors through cephalic or jugular vein and Babesia spp. was diagnosed by examination of blood smear stained with Giemsa stain. Of 173 dogs, 9 dogs (5.2%) were infected with the babesia spp. This result was a little lower than the prevalence of Babesia spp. in dogs of other areas.

• Korean Journal of Veterinary Research
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• v.27 no.2
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• pp.307-316
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• 1987

This study was conducted to identify Babesia sp. strain isolated from the imported beef cattle, Aberdeen-angus heifers, in J farm of Jangsu Prefecture, Cheon-buk Province of Korea, morphologically, etiologically and serologically. Babesia sp. strain was purely isolated through the serial blood passages of three generations against splenectomized calves and one generation of blood passage against non-splenectomized calf(intact calf) by inhibiting the appearance of Theileria sergenti in the blood stream by means of injection of 20% oil pamaquine intramuscularly. In the splenectomized calves, the parasite multiplied markedly in blood stream soon after inoculation and parasitaemia was much severe. An elevated body temperature, anorexia, severe anaemia and icterus were observed clinically. Of three splenectomized calves, two were affected with haemoglobinuria and died. But in the non-splenectomized calf the clinical signs and parasitaemia were very mild. The means of the incubation period, the highest parasitaemia, the highest body temperature and the lowest PCV were 3.5 days, 41.1%, ${42^{\circ}C}$ and 9%, respectively, in the splenectomized calves. In calf erythrocytes Babesia sp. protozoa were polymorphic showing the round, oval, ameboid, piriform and paired piriform etc. The sizes of protozoa were $2.51{\sim}3.91{\times}1.32{\sim}2.19{{\mu}m}$ ($3.20{\times}1.60{\mu}m$). Serologically the isolated Babesia sp. were compared with other parasites, B. ovata, B. bigemina, B. bovis, T. sergenti, A. marginale and A. centrale by using the complement fixation(CF) test. As a result, the antibody titer between the homologous species were high. It was two tubes or more in the CF test. From the results obtained above the pure isolated Babesia species was identical with Babesia ovata Minami and Ishihara, 1980.

• Korean Journal of Veterinary Research
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• v.17 no.2
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• pp.51-61
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• 1977

The smears and tissue sections of organs (liver, lung, kidney, heart, lymph-node, brain) were carried out to compare the detection of parasites in the splenectomized calves artificially infected with B. bigemina and B. argentina, respectively. The results obtained from this experiment were abstracted as follows: 1. In the calves infected with B. argentina the parasites were easily detected by the blood smears and tissue smears but, particularly, the detection of parasites in the kidneys and brain by tissue smears and tissue sections was greater useful. In this experiment, the packing of the parasite-infected red blood cells was found in the capillary vessels of the organs. 2. When the fresh infected-brain was kept at $5^{\circ}C$ refrigerator, Babesia argentina could be detected during 9 days from the brain smears. According to this results, we could do differentiate the parasites from the brain of the dead animal by the doubtful babesiosis in the field. 3. If we use the brain smears and brain sections in order to survey or detect Babesia infections of cattle in Korea, these methods could be worthwhile for the differential diagnosis of B. argentina and B. bigemina.

• Parasites, Hosts and Diseases
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• v.61 no.1
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• pp.72-77
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• 2023

Human babesiosis is a tick-borne disease induced by the genus Babesia and has been significantly reported in the Republic of Korea. This report shows the cases of 2 patients with human babesiosis who traveled to the USA in 2019. The 2 patients experienced fever and had travel histories to babesiosis-endemic regions. The diagnoses of both cases were verified by the identification of Babesia-infected red blood cells on blood smears. One patient was found to be infected with Babesia microti using polymerase chain reaction (PCR) for 18S rRNA, which discovered the phylogenetic link to the B. microti strain endemic in the USA. The 2 patients recovered from fever with subsequent hemoparasite clearance. Babesiosis could be diagnosed in anyone with histories of travel to babesiosis-endemic countries and tick bites. Furthermore, Babesia-specific PCR is required for determining geno-and phenotypic characteristics.

• Korean Journal of Veterinary Research
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• v.39 no.5
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• pp.965-973
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• 1999

This study was carried out to investigate the effects of vitamin E on the prevention and treatment of Babesia gibsoni. Fifteen mongrel dogs, uninfected with Babesia spp, were assigned to three groups according to vitamin E(${\alpha}-tocopherol$) concentrations in the RBC. The concentrations in each of the three groups were, respectively : ${\alpha}-tocopherol$ in RBC less than $30{\mu}g/{\mu}l$(Group I), $30{\mu}g/{\mu}l{\sim}60{\mu}g/{\mu}l$(Group II), more than $60{\mu}g/{\mu}l$(Group III). Artificial infection was accomplished by injecting $2{\times}10^7{\sim}2{\times}10^8$ erythrocyte of Babesia gibsoni-infected dog into the cephalic vein. We investigated the clinical signs, vitamin E concentrations in RBC and serum, Vitamin A concentrations in serum, hematological values, white blood cell(WBC) viability and RBC membrane osmotic fragility after infection of Babesia gibsoni for a period of 20 days at 5 day intervals. The results obtained are summarized as follows : 1. After infection by Babesia gibsoni, clinical examination revealed depression, anorexia, pale mucous membranes, dark brown urine and diarrhea in proportion as time went on. After 10 days of infection, one dog each of Groups I, II and III revealed depression and anorexia. Two dogs in Group I and one dog each of Groups II and III showed dark brown urine after 15 days. Diarrhea was observed in one dog in each of the 3 groups after 20 days of infection. 2. After 5 days of infection, two dogs in each of Groups I, II and III showed Babesia gibsoni in RBC of blood smear stained with Giemsa. At the 15th day after infection with Babesia gibsoni, they were observed in all experimental animals. After both 5 days and 10 days of infection, the rate of Babesia gibsoni parasitized RBC(permillage, ‰) was 1‰, and increased as time went on. 3. After 5 days of infection by Babesia gibsoni, Group I, which had the lowest vitamin E concentration, showed significantly decreased RBC and PCV levels(p < 0.01). Group II and group III also showed significantly decreased RBC and PCV levels after 15 days of infection(p < 0.05). Particularly after 10 days of infection, Group I showed lower values in RBC and PCV levels compared to Groups II and III. WBC, RBC, fibrinogen and total protein levels between the groups did not differ during experimental periods. 4. According to the WBC differential counts, the ratios of neutrophil to lymphocyte showed a tendency to be slightly higher in Group III (more than $60{\mu}g/{\mu}l$) than in Groups I and II. 5. WBC viability did not differ between the groups. 6. RBC membrane osmotic fragility did not differ between the groups.

• Parasites, Hosts and Diseases
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• v.49 no.4
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• pp.437-440
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• 2011

Babesia spp. were detected from 4 asymptomatic pukus captured on a game ranch in central Zambia in October 2008. Blood smears were examined in 4 species of aymptomatic free-ranging antelopes, namely the puku (Kobus vordanii), reedbuck (Redunca arundinum), bushbuck (Tragelaphus sylvaticus), and kudu (Tragelaphus strepsiceros), and showed the presence of Babesia parasites only in the puku. In the puku, the prevalence of babesiosis was estimated at 33.3% (n=12), while the overall prevalence in all examined animals was 8.5% (n=47). The parasites showed morphological characteristics of paired ring-like stages with the length varying between $1.61{\mu}m$ and $3.02{\mu}m$ ($mean=2.12{\mu}m$, n=27; $SD=0.76{\mu}m$). Both the infected and non-infected pukus showed good body condition scores (BCS), while the dominant tick species detected from all animals were Rhipicephalus appendiculatus, Rhipicephalus spp., and Boophilus spp. To our knowledge this is the first report of Babesia spp. infection in pukus in Zambia. These findings suggest that wildlife could play an important role in the epidemiology of babesiosis in Zambia.

• Journal of the korean veterinary medical association
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• v.20 no.3
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• pp.161-168
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• 1984

The clinical observation of the Babesia gibsoni which was broken out in the Shep-herd dogs by Haemaphysalis longiconis was surveyed around the mountain area of Hap Gok Ri in Gan Won Do through year. The results observed were as follows; 1. The ticks appea

• Journal of Veterinary Clinics
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• v.17 no.2
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• pp.346-348
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• 2000

In previous studies, high fever, inappentence, increased respiratory rate, muscle tremors, anemia, jaundice, loss of weight hemoglobinenuria and hemoglobinuria were found in dogs infected intraerythrocytic protzoan parasites of the gennus Babesia. These changes were mainly caused by the intravascular hemolysis. In this study, a Pit Bull Terrier, which was infected by babesia, was investigated clinical signs and patterms of blood. The findings from this study indicated that dog with severs clinical signs shown erythropenia, lymphocytophilia, neutropenia, thrombocytopenia and eosinpenia. Furthermore, it presenter low level of hemglobin and hematocrit. However MCV, MCH and MCHC did not decreased as compare to normal dogs.

• Parasites, Hosts and Diseases
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• v.40 no.1
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• pp.33-40
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• 2002

Attempts were made to isolate and identify Korean bovine Babesia parasite. Blood samples were collected from Holstein cows in Korea. and Babesia parasites were propagated in SCID mice with circulating bovine red 1)food cells for isolation. The isolate was then antigenically and genotypically compared with several Japanese isolates. The Korean parasite was found to be nearly identical to the Oshima strain isolated from Japanese cattle, which was recently designated as Babesia ovata oshimensis n. var. Haemaphusalis longicornis was the most probable tick species that transmitted the parasite .