• Title/Summary/Keyword: Bemisia tabaci

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Feeding Behaviors of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) and Changing of Feeding Behaviors to Cyantraniliprole (EPG를 이용한 담배가루이(Bemisia tabaci)의 섭식행동과 Cyantraniliprole에 대한 섭식변화)

  • Kwon, Hye-Ri;Youn, Young-Nam
    • Korean Journal of Agricultural Science
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    • v.41 no.2
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    • pp.119-124
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    • 2014
  • Feeding behaviors of the sweet potato whitefly, Bemisia tabaci, and changing of their feeding behaviors were recorded and analyzed with an Electrical Penetration Graph (EPG) analysis against cyantraniliprole. The characteristic patterns of feeding behaviors investigated were as follows; NP (non probing), pathway phase waveform, waveform of feeding xylem. In results, B. tabaci did not almost feeding the phloem. And It was not appeared potential drop during recording time. It was predominantly indicated pathway activity waveform and waveform of xylem feeding. After treatment of cyantraniliprole with recommended concentrations to tomato plants, EPG waveforms were recorded during 3 hours. Cyantraniliprole treatment showed longer time to first feeding xylem than untreated (P=0.043). It was showed shorter duration of first feeding xylem than untreated (P=0.017). And it was showed longer NP (non-probing) time than untreated. Consequently, It was supposed that cyantraniliprole was effect of anti-feeding or avoidance to B. tabaci.

Morphological Characteristics of Bemisia tabaci(Gennadius) (Homoptera: Aleyrodidae) and Discrimination of Their Biotypes in Korea by DNA Makers (담배가루이 Bemisia tabaci(Gennadius)(Homoptera: Aleyrodidae)의 형태적 특징과 DNA 표식자에 의한 biotype 판별)

  • 이명렬;안성복;조왕수
    • Korean journal of applied entomology
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    • v.39 no.1
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    • pp.5-12
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    • 2000
  • The sweetpotato whiteflies, Bemisia tabaci(Gennadius), were found recently in Korea on Glycine max, Euphorbia pulcherrima, and Rosa hybrida. The biotype identity of Bemisia tabaci in Korea was determined by several DNA markers including the random amplified polymorphic DNAs, and restriction fragments length polymorphism of mitochondrial 12S and 16S rRNA genes. The electromorph profiles of DNA fragments from the rose(Jincheon) and poinsettia(Seoul) populations in Korea are both identical to those of B biotypes distributed in Australia, Israel, and Japan. The populations of B. tabaci collected on Glycine max, Ipomea batatas, and Perilla frutescens in different localities retained the same DNA markes with the population from Lonicera japonica and shikoku of Japan. These populations are non-B biotype and considered as an indigenous type in the Far Eastern Asia Region including Korea and Japan, Morphological Characteristics of B. Tabaci were also observed by the scanning electron microscope and described with the comparison to the other important whitefly pest, Trialeurodes vaporariorum (Westwood).

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Change of population density of tobacco whitefly (Bemisia tabaci, Aleyrodidae, Hemiptera) by RNAi (RNAi에 의한 담배가루이(Bemisia tabaci, 가루이과, 노린재목)의 개체군 밀도변화)

  • Ko, Na-Yeon;Youn, Young-Nam
    • Korean Journal of Agricultural Science
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    • v.42 no.1
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    • pp.7-13
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    • 2015
  • Ninety genes randomly selected from tobacco whitefly (Bemisia tabaci) cDNA library was studied for selecting target gene in order to control of tobacco whitefly using TRV-VIGS vector (tobacco rattle virus-virus induced gene silencing vector) with RNAi. First of all, the occurrence of B. tabaci adult according to agro-infiltration of TRV was no significant difference. And that of TRV inserted tobacco whitefly cDNA showed a significant difference in each sample. P CV and N CV sample were more than 80% could be confirmed in 5 samples, for example, wh11, wh36, wh46, wh50 and wh71. Lastly, the occurrence of nymph and egg also showed a significant difference in each sample. That could be confirmed in 11 samples, for example, wh01, wh09, wh10, wh15, wh16, wh23, wh24, wh48, wh64 and wh66. In case of wh46, wh50 and wh71 sample could be confirmed that occurrence of B. tabaci adult was many, but occurrence of B. tabaci nymph and egg was a little. So sample showed a physioecological good effect to control of whitefly need to be investigated variation of gene expression in whitefly body using qRT-PCR through individual test.

Selection of Lecanicillium Strains with High Virulence against Developmental Stages of Bemisia tabaci

  • Park, Hee-Yong;Kim, Keun
    • Mycobiology
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    • v.38 no.3
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    • pp.210-214
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    • 2010
  • Selection of fungal strains with high virulence against the developmental stages of Bemisia tabaci was performed using internal transcribed spacer regions. The growth rate of hyphae was measured and bioassay of each developmental stage of B. tabaci was conducted for seven days. All of the fungal strains tested were identified as Lecanicillium spp., with strain 4078 showing the fastest mycelium growth rate (colony diameter, 16.3 $\pm$ 0.9 mm) among the strains. Compared to strain 4075, which showed the slowest growth rate, the growth rate of strain 4078 was increased almost 2-fold after seven days. Strains 4078 and Btab01 were most virulent against the egg and larva stages, respectively. The virulence of fungal strains against the adult stage was high, except for strains 41185 and 3387. Based on the growth rate of mycelium and level of virulence, strains 4078 and Btab01 were selected as the best fungal strains for application to B. tabaci, regardless of developmental stage.

A Report on Mixed Occurrence of Tobacco Whitefly (Bemisia tabaci) Biotypes B and Q in Oriental Melon Farms in Kyungpook Province, Korea (담배가루이 생태형 B와 Q가 같이 발생하는 경북 참외재배 지역 보고)

  • Kim, Eunsung;Kim, Yonggyun
    • Korean journal of applied entomology
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    • v.53 no.4
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    • pp.465-472
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    • 2014
  • The tobacco whitefly, Bemisia tabaci, infest the Oriental melon and give significant economic damage along with its virus-vectoring activity. Various biotypes of B. tabaci have been well known and are classified depending on the severity of crop damage and insecticide susceptibility. In this study, B. tabaci adults were collected in the melon fields located in Poongchun-myeon, Andong, Korea and diagnosed on their biotypes using PCR molecular markers. From the all the 11 greenhouses, B. tabaci biotype Q was identified. In addition, biotype B adults were also found from the 4 greenhouses. These results report the first occurrence of B. tabaci at the Oriental melon farms in Gyeongbuk province with mixed infection by the two biotypes in the area.

Occurrence of Bemisia tabaci JpL (Hemiptera: Aleyrodidae) on Jeju Island in Korea (제주도 지역 담배가루이 JpL의 발생현황)

  • Park, Yujeong;Nam, Hwa Yeun;Lee, Joon-Ho
    • Korean journal of applied entomology
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    • v.58 no.3
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    • pp.203-207
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    • 2019
  • Bemisia tabaci (Hemiptera: Aleyrodidae) is one of the major insect pests causing economic damages to over 900 different crops in the world. Especially, B. tabaci JpL (L. japonica) is known as a species living only in Japan and Korea so far, and mainly in Lonicera japonica and specific plant species. This study was conducted to investigate the occurrence and spread of B. tabaci JpL on Jeju Island in 2019. Of the total 25 areas surveyed, 1,003 individuals of B. tabaci JpL adults were collected from 24 L. japonica and one Conyza sumatrensis. All populations identified belonged to the JpL species. The result of this study showed that the population of B. tabaci JpL has increased at very high speeds to date compared to the previous reports in Korea.

Reassessment of the Taxonomic Status of the Bemisia tabaci Complex (Hemiptera: Aleyrodidae) Based on Mitochondrial COI Gene Sequences (미토콘드리아 COI 유전자 분석을 통한 담배가루이 종복합군의 분류학적 재평가)

  • Lee, Wonhoon;Lee, Gwan-Seok
    • Korean journal of applied entomology
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    • v.56 no.2
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    • pp.107-120
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    • 2017
  • Bemisia tabaci (Hemiptera: Aleyrodidae) is one of the most important insect pests in the world. In the present study, the taxonomic status of B. tabaci and the number of species composing the B. tabaci complex were determined based on 550 COI gene sequences of B. tabaci. Genetic divergence within B. tabaci ranged from 0% to 27.8% (average 11.1%). This result indicates that the B. tabaci complex is composed of multiple species that may belong to different genera or subfamilies. A phylogenetic tree constructed based on 217 COI gene sequences without duplications revealed that the B. tabaci complex is composed of a total of 43 putative species, including a new species, Java. In addition, genetic divergence within nine species (Australia, Asia II 1, Asia II 6, Asia II 7, Asia II 10, Mediterranean, New world, New world 2, Sub Saharan Africa 1) indicates that 4.0% is reasonable to be used as a threshold of species boundaries within the B. tabaci complex, and species with high intraspecific genetic divergences can be related with cryptic species.

Efficiency of LED Trap on Controlling Tobacco Whitefly, Bemisia tabaci Adults in Greenhouse (온실에 발생하는 담배가루이 성충에 대한 LED 트랩 방제효과)

  • Jeon, Ju-Hyun;Lee, Sang-Guei;Lee, Hoi-Seon
    • Journal of Applied Biological Chemistry
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    • v.57 no.3
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    • pp.243-245
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    • 2014
  • To evaluate light-emitting diode (LED) as potential attractants for Bemisia tabaci adults, attractiveness of white and yellow LED traps were investigated in greenhouse. The yellow LED trap showed the most attractive to B. tabaci adults, followed by a similarly attraction to the white LED trap, whereas the control (no light trap) was little attractive to B. tabaci adults. These results suggested that yellow and white LED traps could be used for environment-friendly insect pest control.

Selection of Entomopathogenic Fungi Against Bemisia tabaci and Culture Characterization on Spent Coffee Grounds Medium (담배가루이에 대한 곤충병원성 곰팡이 선발 및 커피박 배지에서의 배양 특성 )

  • Jang, Ji-Won;Heo, In-Ji;Hwang, Dong-Young;Kim, Seul-Ki;Shin, Tae-Young
    • Korean Journal of Organic Agriculture
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    • v.31 no.4
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    • pp.427-439
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    • 2023
  • The silverleaf whitefly, Bemisia tabaci, is a major pest distributing worldwide damaging over 900 host plant species, and is highly resistant to chemical pesti- cides. Due to the high pesticide resistance of whitefly, there is a need for alternatives to chemical control. Entomopathogenic fungi are candidates for biological pesticide that can overcome the resistance problem of chemical pesticide. Therefore, in this study, we tested pathogenicity of the entomopathogenic fungi to select high insec- ticidal activity against whitefly. As a result, IPBL-C (Cordyceps fumosorosea) and IPBL-F (Metarhizium pinghaense) isolates showed high insecticidal activity against whitefly. Additionally, as a result of culturing the selected isolates on spent coffee grounds medium, the conidia of IPBL-F produced on coffee grounds medium showed five times higher heat stability after heat treatment at 45℃ for one hour than conidia produced on PDA medium.

Effect of Cooking Oil and Yolk Mixture on Control of Bemisia tabaci on Fruit Vegetables (난황유를 이용한 과채류 발생 담배가루이 방제)

  • Park, Jong-Ho;Hong, Sung-Jun;Han, Eun-Jung;Shim, Chang-Ki;Kim, Min-Jeong;Kim, Yong-Ki;Kim, Seok-Cheol
    • Korean Journal of Organic Agriculture
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    • v.25 no.1
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    • pp.149-159
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    • 2017
  • This study was conducted to develop an organic control method of tobacco whitefly (Bemisia tabaci) by using cooking oil and yolk mixture (COY). Mortality rate against B. tabaci nymph on paprika leaf was 94.5% by COY treatment and it was not significantly different from that by pyridaben treatment. Mortality rate against B. tabaci nymph on tomato leaf was 41.4% by COY treatment and it is lower than that by neem extract. But mortality rates of B. tabaci adult by COY and neem extract were no significant differences. Oviposition of B. tabaci were decrease respectively 75.8% and 52.7% by COY on paprika and tomato leaves. In paprika and tomato greenhouses, damaged by B. tabaci, COY was sprayed twice and resulted in respectively 100% and 38% control values.