• The Transactions of The Korean Institute of Electrical Engineers
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• v.57 no.1
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• pp.137-141
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• 2008

Biochip, which implements bioanalytical process on a tiny surface, is one of candidates for medical diagnosis, drug screening, and molecular sensing. In general, a type of biochip based on microfluidics is composed of microcomponents including microchannel, pump, and valve, which require complicated processes. In this study, gray-scale photolithography(GSPL) was applied to fabricate a biochip with multiple layers. A mould for casting PDMS(polydimethylsiloxane) channel, was fabricated using GSPL. A gray-photomask was prepared by printing gray patterns on a high-quality glossy paper followed by photoreducing by 10:1 onto the photo-film. The formation of multiple layers was studied according to the change of gray level of pattern and the developing time. A biochip composed of a weir(multiple layer structure) and a reaction chamber in a single microchannel was fabricated in a glass plate. Finally, we investigated the application of biochip to antigen-antibody reaction by packing the microbead coated with antibody.

• Proceedings of the KIEE Conference
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• 2003.11c
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• pp.488-491
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• 2003

Biochip-based diagnostic technology is an effective, time- and money- saving way. But, biochip analyzer including CCD imaging system has a complete optical system. It is one of reasons that the cost of biochip analyzer is expensive with CCD imaging system. In this paper, We suggested the simple and effective optical system for biochip analyzer with CCD imaging system. It consists of two parts with the same structure but opposite direction. Each part consists of achromatic doublet and meniscus. Suggested optical system has less lenses than existing system and more efficiently.

• BMB Reports
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• v.46 no.5
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• pp.270-275
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• 2013

It is essential to analyze rare mutations in many fields of biomedical research. However, the detection of rare mutations is usually failed due to the interference of predominant wild-type DNA surrounded. Herein we describe a sensitive and facile method of detecting rare point mutation on the basis of allele-specific amplification in emulsion PCR. The identification and selective amplification of rare mutation are accomplished in one-pot reaction. The allele-specific primers coupled on magnetic beads allow the exclusive amplification and enrichment of the mutant amplicons. The productive beads bearing mutant amplicons are subsequently stained with the fluorescent dyes. Thus, the rare point mutations with a percentage as low as 0.1%, can be detected by fluorescent analysis. The relative percentages of mutation among different samples can be roughly accessed by counting the fraction of fluorescent positive beads through flow cytometry.

• Proceedings of the KSME Conference
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• 2003.04a
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• pp.1073-1077
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• 2003

This study aims to investigate the fabrication process of nano silicon mold using electron beam lithography (EBL) to generate the nanometer level patterns by nano-imprinting technology. the nano-patterned mold including 100mm pattern size has been fabricated by EBL with different doses ranged from 22 to 38 ${\mu}C/cm^2$ on silicon using the conventional polymethylmetharcylate(PMMA) resist. The silicon mold is fabricated with various patterns such as circles, rectangles, crosses, oblique lines and mixed forms, The effect of dosage on pattern density in EBL is discussed based on SEM (Scannning Electron Microscopy) analysis of fabricated molds. The mold surface is modified by hydrophobic fluorocarbon (FC) thin films to avoid the stiction during nano-imprinting process.

• Design & Manufacturing
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• v.2 no.2
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• pp.25-28
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• 2008

Micro fabrication of biochip such like lab-on-a-chip becomes increasingly important. In this study, we designed and manufactured of new molds which were main factors for forming process in order to mass produce of biochip using forming process. Forming analysis of biochip was performed by Moldflow software. Results of this study are able to design and manufacture the mold which can be easy to eject the workpiece by using the slide mechanism for biochip.

• Proceedings of the Korean Society Of Semiconductor Equipment Technology
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• 2004.10a
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• pp.33-48
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• 2004

• Proceedings of the Korean Society for Bioinformatics Conference
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• 2006.02a
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• pp.63-69
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• 2006

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2005.07a
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• pp.655-656
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• 2005

In the future, plastic based system will play a crucial role in modem life, for examples, transparent display or disposable electronics and so on. In this paper, we introduced a new method to fabricate the metal line on the plastic substrate. Metal lines were fabricated by hot embossing and CMP process on PMMA (polymethylmethacrylate) substrates. A Si mold was made by wet etching process and a PMMA wafer was cut off from I mm thick PMMA sheet. A 100 nm thick Al was deposited on PMMA wafers. The Al deposited PMMA wafer and the Si mold carefully sandwiched which was directly imprinted by hot embossing. After imprinting process, a residual Al layer was removed by CMP process. Finally, we found the entire process may be very useful to fabricate the metal line on plastic substrates.

• Proceedings of the Korean Operations and Management Science Society Conference
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• 2004.05a
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• pp.155-158
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• 2004

The aim of this study is to map the structure of biochip research field and analyse ex-ante feasibility in R&D planning stage using Co-word analysis. We used SCI Database as a source data to analyze the intellectual structure of biochip research in two different periods (1994${\sim}$1999, 1994${\sim}$2002).

• Journal of Korea Technology Innovation Society
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• v.6 no.4
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• pp.508-517
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• 2003

Biochip, that is a fusion of BT/IT/NT, is the fundamental technology of bio-engineering in Post-genome Era. It is not only the tool to analyze the huge bio-informations but also the technology necessary for screening of new medicine and medical examination. Except some technological fields, biochip area has a good chance of occupying advantageous position in the world technology community by early aggressive investment, because a new access to this technology is possible without a past imitation process. This study is to analyze the trend of development of domestic and foreign biochip technology and its current market status. Finally it presents some suggestions for the government policy considering the possibility of its development in Korea.