• Title, Summary, Keyword: Biocompatibility

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Study on Biocompatibility and Mineralization Potential of Capseal

  • Bae, Kwang Shik;Chang, Seok Woo;Kum, Kee Yeon;Lee, Woo Cheol
    • Journal of Korean Dental Science
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    • v.7 no.1
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    • pp.1-5
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    • 2014
  • Purpose: Capseal I and Capseal II are calcium silicate and calcium phosphate based experimental root canal sealers. This study sought to evaluate the biocompatibility and mineralization potential of Capseal I and Capseal II. Materials and Methods: The biocompatibility and mineralization related gene expression (alkaline phosphatase [ALP], bone sialoprotein [BSP], and osteocalcin) of Capseal I and Capseal II were compared using methylthiazol tetrazolium assay and reverse transcription-polymerization chain reaction analysis, respectively. The results were analyzed by Kruskal-Wallis test. A P-value of <0.05 was considered significant. Result: Both Capseal I and Capseal II were favorable in terms of biocompatibility, influencing the messenger RNA expression of ALP and BSP. Conclusion: Within the limitation of this study, Capseal is biocompatible, with mineralization promoting potential; thus, it could be a promising root canal sealer.

Characterization of Silk Fibroin/S-carboxymethyl Kerateine Surfaces: Evaluation of Biocompatibility by Contact Angle Measurements

  • Lee, Kuen-Yong
    • Fibers and Polymers
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    • v.2 no.2
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    • pp.71-74
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    • 2001
  • Surface characterization of materials has been considered critical in the development of biomaterials, as many unfavorable responses from the body occur at the interface between a material and the body component. The contact angle measurement is one means to characterize the surface properties and to correlate them to the biocompatibility of materials. In this paper, surface characteristics of silk fibroin/S-carboxymethyl kerateine, representative fibrous proteins, were investigated by contact angle measurements of ESCA. The biocompatibility of the blends was evaluated based on minimal interfacial free energy concept, and compared with other potential biomaterials. It was also hypothesized that the enhanced surface polarity of the blends was generated from the conformational transition of proteins. This approach to evaluate the biocompatibility of materials based on surface characteristics may find wide utility in many biomedical applications.

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Effect of Bioactive Glass Addition to the TTCP/DCPA Based Injectable Bone Substitute for Improved Biocompatibility

  • Sadiasa, Alexander;Sarkar, Swapan Kumar;Franco, Rose Ann;Yang, Hun-Mo;Lee, Byong-Taek
    • Proceedings of the Materials Research Society of Korea Conference
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    • pp.52.1-52.1
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    • 2011
  • In this work, the effect of the addition of bioactive glass in the biocompatibility and mechanical behavior of conventional TTCP/DCPA based bone cement were investigated. The cement was initially modified with chitosan and HPMC which cross-linked with citric acid to improved mechanical properties.The injectable bone substitutes were further modified by adding varying amounts of bioactive glass (0%, 10%, 20% and 30%) and its effects on the biocompatibility of the material were studied. Afterbio-glass powders were mixed with the optimized composition for HPMC and citric acid content,the IBS was incubated at $37^{\circ}C$ at different time intervals and showed progressive formation of HAp with increasing time. Mechanical properties like Vickers hardness and compressive strength were found to increase with the increasing amount of bioactive glass addition and that setting time was shortened. The fabricated IBS morphologies were further characterized using SEM. MTT assay was performed to check the cell cytotoxicity and cell proliferation for 1, 3 and 5 days. Cell morphology, adhesion and proliferation behavior of cell in the IBS by culturing MG-63 cells on the IBS for 20, 60 and 90 mins and 1, 3 and 5 days was also investigated. All the results showed increasing biocompatibility as the bioglass content increased. MTT results found the materials to be cytocompatible and SEM images showed that cells attached and proliferated successfully.

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A Study for Biocompatibility of Acupuncture′s Metal Materials, - Focused on Biological Character - (침 금속재료에 대한 생체적합성 연구 -생물학적 특성을 중심으로-)

  • Baek Yong-Hyeon;Chung In-Tae;Lee Sang-Hoon;Lee Jae-Dong;Choi Do-Young
    • The Journal of Korean Medicine
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    • v.25 no.3
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    • pp.180-190
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    • 2004
  • Objectives : To present criteria for acupuncture standardization, which will improve quality of acupuncture and secure safety, through studies of the biocompatibility of acupuncture materials. Methods : Acupuncture needles distributed in Korea were studied. Intracutaneous reaction tests and sterility tests were executed to study the biocompatibility of acupuncture. Results & Conclusions : All 4 kinds of acupuncture needles distributed in Korea were found suitable for medical use, through intracutaneous reaction tests and sterility tests. However, the medical instrument standards of the Korea Food and Drug Administration lack criteria concerning intracutaneous reaction tests and sterility tests. The criteria for these tests should be standardized in order to secure the biocompatibility of acupuncture needles.

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Comparative Biocompatibility of Metal Implants in Connective Tissue of Abdominal Wall of the Mouse (마우스의 복벽에서 결합조직에 대한 금속 이식체의 생체적합성 비교)

  • 김국렬;이민호;김병일;민병운;김명훈;최은상;조현욱
    • Toxicological Research
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    • v.20 no.1
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    • pp.13-20
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    • 2004
  • The purpose of this study is to compare the biocompatibility of commercial purity Ti, Ti-6AI-4V and Ti-6AI-7Nb alloy specimens with and without surface treatment in mouse abdominal connective tissue in vivo. Each metal was implanted into specific abdominal subdermal tissue site of female mouse. After 4 weeks, the implants were removed and abdominal tissues were fixed, dehydrated and embedded in glycol methacrylate resin. And the tissues were histologically prepared for microscopical evaluation. It was characterized by the presence of connective tissue with fibrous capsule surrounding the implant. The fibrous tissue surrounding the implant was studied to determine the biocompatibility of implanted metals. The average thickness of the fibrous capsule formed around the implant was much thinner for the hydrogen peroxide added hydrochloric acid solution-treated specimen than for the others. The results of this evaluation indicate that modification of the surface properties of titanium and titanium alloy implants changes the biological properties in the abdominal connective tissue. In conclusion, these observations suggest that the proper surface treatment performed in the study is effective for the improvement of biocompatibility.

A Study of Biocompatibility of Acupuncture's Metal Materials - Focused on Biological Character (2) - (침 금속재료에 대한 생체적합성 연구 - 생물학적 특성을 중심으로(2) -)

  • Jung In-Tae;Kang Jung-Won;Baek Yong-Hyeon;Lee Sang-Hoon;Lee Jae-Dong;Choi Do-Young
    • The Journal of Korean Medicine
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    • v.26 no.2
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    • pp.166-175
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    • 2005
  • Objecitve: To present criteria for acupuncture standardization, which will improve the quality of acupuncture and secure safety, through studies of the biocompatibility of acupuncture needles. Methods: Acupuncture needles distributed in Korea were studied. Acute poisonous tests and pyrogenicity materials tests were executed to study the biocompatibility of acupuncture needles. Results & Conclusions: All 3 brands of acupuncture needles commonly distributed in Korea were found suitable for medical use, through acute poisonous tests and pyrogenicity materials tests. However, the medical instrument standards of the Korea Food and Drug Administration for acupuncture lack criteria concerning acute poisonous tests and pyrogenicity materials tests. The criteria for these tests should be standardized in order to secure the biocompatibility of acupuncture needles.

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Biocompatibility of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Copolyesters Produced by Alcaligenes sp. MT-16

  • Choi, Gang-Guk;Kim, Hyung-Woo;Kim, Young-Baek;Rhee, Young-Ha
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.10 no.6
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    • pp.540-545
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    • 2005
  • Poly(3-hydroxybutyrate-co-3-hydroxyvalerate), poly(3HB-co-3HV), copolyesters, with 3-hydroxyvalerate (3HV) contents ranging from 17 to 60 mol%, were produced by Alcaligenes sp. MT-16, and their biocompatibility evaluated by the growth of Chinese hamster ovary (CHO) cells and the adsorption of blood proteins and platelets onto their film surfaces. The number of CHO cells that adhered to and grew on these films was higher with increasing 3HV content. In contrast, the tendency for blood proteins and platelets to adhere to the copolyester surfaces significantly decreased with increasing 3HV content. Examination of the surface morphology using atomic force microscopy revealed that the surface roughness was an important factor in determining the biocompatibility of theses copolyesters. The results obtained in this study suggest that poly(3HB-co-3HV) copolyesters, with >30 mol% 3HV, may be useful in biocompatible biomedical applications.

A review of biocompatibility of zirconia and bioactivity as a zirconia implant: In vivo experiment (지르코니아의 생체적합성과 임플란트로서의 생체활성에 대한 연구: In vivo 실험 문헌 고찰)

  • Suh, Da-Won;Kim, Young-Kyun;Yi, Yang-Jin
    • The Journal of Korean Academy of Prosthodontics
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    • v.57 no.1
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    • pp.88-94
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    • 2019
  • Increasing demands for esthetic dental treatment, zirconia, which has high mechanical and esthetic properties, had been applied more and more in clinics. Therefore, assessment of biocompatibility of zirconia is necessary. In this article, a review of in vivo studies of zirconia compatibility was performed. In vivo studies showed zirconia had great biocompatibility both on soft and hard tissue. Studies with various animals and patients reported high biocompatibility of zirconia. In terms of bone synthesis and bone adhesion, zirconia showed similar biocompatible properties to titanium. On the other hand, zirconia could be used as implant. For using as an implant, various methods of Hydroxyapatite (HA) coating had been suggested. Since HA coating on titanium implant showed some problems such as low bonding strength and degeneration of HA, HA-zirconia composite, HA-coated zirconia, and HA-zirconia functionally graded material (FGM) or intermediate layer of alumina had been proposed. These methods showed higher bonding strength and biocompatibility.

A review of biocompatibility of zirconia: In vitro experiment (지르코니아의 생체적합성에 대한 연구: In vitro 실험 문헌 고찰)

  • Suh, Da-Won;Kim, Young-Kyun;Yi, Yang-Jin
    • The Journal of Korean Academy of Prosthodontics
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    • v.56 no.4
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    • pp.391-395
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    • 2018
  • Increasing demands for zirconia material in clinics, assessment of biocompatibility of zirconia is essential. In this article, a review of in vitro studies of zirconia compatibility was performed. Zirconia showed great biocompatibility at in vitro studies with various cell lines such as fibroblasts, osteoblasts, and lymphocytes. Many studies reported that zirconia caused no cytotoxicity or mutation. Zirconia also showed less bacterial adhesion. There were no adverse effects except for small reduced strength with in vitro study mimicking long-term exposure of body fluid. According to the study with ostoblast-like cells, zirconia could regulate genes of immunity, molecular transport, and cell cycle. Such gene regulating was considered as one of the reasons of zirconia biocompatibility. With biocompatibility of zirconia powders, in vitro studies had controversial conclusions. It seems that zirconia powders might have cytotoxicity.

The biocompatibility and mechanical properties of plasma sprayed zirconia coated abutment

  • Huang, Zhengfei;Wang, Zhifeng;Yin, Kaifeng;Li, Chuanhua;Guo, Meihua;Lan, Jing
    • The Journal of Advanced Prosthodontics
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    • v.12 no.3
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    • pp.157-166
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    • 2020
  • PURPOSE. The aim of this study was to evaluate the clinical performance and reliability of plasma sprayed nanostructured zirconia (NSZ) coating. MATERIALS AND METHODS. This study consisted of three areas of analysis: (1) Mechanical property: surface roughness of NSZ coating and bond strength between NSZ coating and titanium specimens were measured, and the microstructure of bonding interface was also observed by scanning election microscope (SEM). (2) Biocompatibility: hemolysis tests, cell proliferation tests, and rat subcutaneous implant test were conducted to evaluate the biocompatibility of NSZ coating. (3) Mechanical compatibility: fracture and artificial aging tests were performed to measure the mechanical compatibility of NSZ-coated titanium abutments. RESULTS. In the mechanical study, 400 ㎛ thick NSZ coatings had the highest bond strength (71.22 ± 1.02 MPa), and a compact transition layer could be observed. In addition, NSZ coating showed excellent biocompatibility in both hemolysis tests and cell proliferation tests. In subcutaneous implant test, NSZ-coated plates showed similar inflammation elimination and fibrous tissue formation processes with that of titanium specimens. Regarding fatigue tests, all NSZ-coated abutments survived in the five-year fatigue test and showed sufficient fracture strength (407.65-663.7 N) for incisor teeth. CONCLUSION. In this study, the plasmasprayed NSZ-coated titanium abutments presented sufficient fracture strength and biocompatibility, and it was demonstrated that plasma spray was a reliable method to prepare high-quality zirconia coating.