• The Korean Journal of Mycology
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• v.21 no.2
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• pp.146-156
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• 1993

Boletus is a symbiotic ectomycorrhizal flesh fungi forming mycorrhizas with trees of Pinaceae, Fagaceae and Betulaceae. The species in the genus have relatively strong host specificity to enhance the growth of host plants and some of them are flavorful. But Korean rarely consumes these kinds of mushrooms and B. edulis has not been reported in this country. In the genus twenty six species have been reported in Korea, but the number is expected to increase as collection efforts are intensified. Keys to the families of Boletaceae and Strobilomycetaceae, to the genus of Boletaceae and to the species of Boletus were provided based on published keys and the descriptions of species reported in Korea. However, the key to the Boletus species did not include all the species occurring in Korea and not all the ones in the key are indigenous.

• The Korean Journal of Mycology
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• v.10 no.4
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• pp.207-212
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• 1982

Three species of Genus Boletus are described and illustrated in this paper. They are Boletus pseudocalopus Hongo, B. quercinus Hongo, and B. laetissimus Hongo and are newly recorded for the Korean fungus flora. Also authors designate their Korean names.

• The Korean Journal of Mycology
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• v.6 no.2
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• pp.11-14
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• 1978

Attempts were made to investigate the sterol components of Boletus aerus Bull. which grows wildly in Korea. Its carpophores were collected in Gyeong-Gi Province and extracted with chloroform and methanol. A sterol was isolated and identified as ergosterol by T.L.C., G.L.C. and chemical tests.

• The Korean Journal of Mycology
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• v.43 no.1
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• pp.68-70
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• 2015

Mushrooms are valued as a nutritional food and also as an important source of useful medicinal components. They produce various secondary metabolites which have interesting biological activities and unique chemical structures. As part of our ongoing investigation on chemical constituents and bioactive components of Korean native mushrooms, compound 1, an indole alkaloid, was isolated from the fruiting body of Boletus umbriniporus. B. umbriniporus is characterized by its yellow flesh, which changes to pallid blue when exposed to air, and its chemical constituent has not been reported. Chemical structure of compound 1 was determined to be flazin on the basis of ESI-mass, $^1H$ NMR, $^{13}C$ NMR, $^1H-^1H$ COSY, HMQC, and HMBC analysis. This compound was isolated from the fruiting body of B. umbriniporus for the first time.

• Journal of Plant Biology
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• v.30 no.4
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• pp.225-247
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• 1987

Meiosis and postmeiotic mitosis in Boletus rubinellus were examined ultrastructurally. Meriosis occurred at the apex of the basidium. A sausage-shaped spindle pole body(SPB) was observed along with the presence of synaptonemal complexes during pachytene and a diglobular SPB was present on late pachytene or diplotene nuclei. During metaphase I, the monoglobular SPB at the spindle pole was surrounded bya membrane and the nuclear enveloope was discontinuous. At anaphase I, the chromosomes became better defined and formed a central spindle. The nucleolus was extruded from the nucleus. During anaphase I, the SPB was excluded from the chromosomal region by a membrane and both poles were fully separated to opposite sides of the basidial wall. In meiosis II, the two nuclei divided synchronously and the spindles were parallel. The spindles were smaller than in meiosis I, while the SPB was approximately the same size as that of the similar stage in meiosis I. During anaphasetelophase II, the SPB was surrounded by a cap of endoplasmic reticulum (ER) that delimited it from the spindle. The postmeiotic interphase nuclei migrated to the mid-region of the basidium before migration to the spores. The SPB at this stage was diglobular. A postmeiotic mitosis occurred within the basidiospore, and the plane of the spindle was obique to the long axis of the spore. The spindle and SPB were smaller than at meiosis I and there were fewer nonchromosomal microtubules. At anaphase, the nucleolus was present inside the nuclear envelope but lateral to the spindle.

• The Korean Journal of Mycology
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• v.4 no.1
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• pp.17-25
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• 1976

Among the higher fungi which wore collected in the Geong Gi Province and Seoul Korea, nine species, i.e., Mycena haematopoda, Amanita griseofarinosa, Pholiota spectabilis. Pholiota vermiflua, Boletus erythroporus, Russula alutacea, Russula chameleontina, Russula xcampelina and Polyporus sulphureus, were found to be unrecorded species in Korea. The morphological, histological and microchemical characteristics of these fungi were reported.

• The Korean Journal of Mycology
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• v.11 no.1
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• pp.9-13
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• 1983

Ectomycorrhizal fungi in Pinus $rigida{\times}taeda$ and poplar stands (Populus $alba{\times}glandulosa$ and others) were collected in 1981 and 1982. A total of 39 mushrooms representing 15 genera were identified from the pine stand, and the major genera were Amanita, Lactarius, Russula, Laccaria, and Boletus. From the poplar stands 16 mushrooms representing 8 genera were identified and 13 of them were the same found in the pine stand. Amanita, Russula, Laccaria, and Leccinum are the major genera in the poplar stands. A single species of Boletus was found in large number in pine and poplar stands. Lactarius was not observed in the poplar stands, while Leccinum was not found in the pine stand. Cantharellus lutescens which has not been reported in Korea was collected in the pine stand.

• Mycobiology
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• v.31 no.1
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• pp.23-31
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• 2003

With universal primer ITS1-F, the specific DHJ2 primer was developed to detect the Ectomycorrhizal(ECM) root tips in soil and to identify the species of ECM fungi, as based on DNA sequences of rDNA stored in GeneBank of NCBI. This primer was designed with the common sites of rDNA of Amanita and Boletus, and was also designed with several DNA programs provided by NCBI. The DNA fragments synthesized by PCR were calculated to be 1,000 to 1,200 bps of DNA located to 18s to 28s rDNA to contain two variable sites of ITS, indicating much diversities for specific species or ecotypes of ECM fungi. The primer DHJ2 reacted with the genomic DNA's extracted from the tissues of basidiocarp at the rate of 73 of 80 fungi collected produced single bands with a 1,100 bps length. The DNA fragment synthesized with the genomic DNA that extracted from eight ECM tips of Pinus densiflora was confirmed and analysized to the rDNAs of ECM in full sequences, and informed to be a ECM fungal species in the forest.

• The Korean Journal of Mycology
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• v.23 no.1 s.72
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• pp.28-36
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• 1995

To investigate the antibacterial activities of 53 species of mushrooms for Gram-positive bacteria, bioactive substances from mushrooms were extracted with petroleum ether, 80% ethanol, and distilled water. The 14 extracts of 12 species of mushrooms, largely with 80% ethanol, showed antibacterial activities against 6 species of Gram-positive bacteria selectively. Especially, the extracts of Thylopilus PS-5, Pulveroboletus PS-7, and Russula PS-9 showed various antibacterial activities against several bacteria. Minimal inhibitory concentration (MIC) of the 80% ethanol extracts from Pulveroboletus PS-7 and Boletus PS-8 was $300\;{\mu}g/ml$ against Bacillus subtilis, and that of the 80% ethanol extracts from Trichaptium PS-3 was $500\;{\mu}g/ml$ against Micrococcus luteus. MIC of other extracts with antibacterial activities was above $1000\;{\mu}g/ml$.

• The Korean Journal of Mycology
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• v.10 no.1
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• pp.15-19
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• 1982

The fungal flora of Mt. Gyeryong National Park was investigated for the foundation of the nature studying garden and the nature conservation of the area during the period of July to October 1981. The fungi growing wildly in the area were collected and classified into 19 families, 53 genera, 93 species of Basidiomycetes and 5 families, 7 genera, 8 species of Ascomycetes. Species of Amanita, Boletus, Suillus, Russula, Lactarius, Laccaria and Marasmius were frequently observed during the hot and humid period. Well-developed communities of Lactarius piperatus, Russula emetica and Ramaria spp. were also found in Mt. Gyeryong. Unrecorded species of fungi in Mt. Gyeryong studied were not included in this report.