• Journal of dental hygiene science
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• v.9 no.4
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• pp.427-433
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• 2009

Nuclear factor I-C (NFI-C) null mice demonstrated aberrant odontoblast differentiation and abnormal dentin formation. In order to elucidate the mechanisms responsible for these changes, we evaluated the expression of dentin matrix gene after over-expression and inactivation of NFI-C in MDPC-23 cells by reverse transcription-polymerase chain reaction (RT-PCR) analysis. Collagen type I (Col I), osteocalcin (OC), and dentin sialophosphoprotein (DSPP) expression was decreased after inactivation of NFI-C. However, bone sialoprotein (BSP) expression was dramatically increased after inactivation of NFI-C. ALP and DMP4 expression was not changed after inactivation of NFI-C. The expression of alkaline phoshatase (ALP) and dentin matrix protein 4 (DMP4) was increased after over-expression of NFI-C, while Col I, OC, DSPP, and BSP expression was decreased. These findings suggest that odontoblasts after loss of NFI-C lost the phenotype of odontoblasts and acquired those of osteoblasts.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.8
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• pp.3301-3306
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• 2015

Nucleolin (C23) is an important anti-apoptotic protein that is ubiquitously expressed in exponentially growing eukaryotic cells. In order to understand the impact of C23 in radiation therapy, we attempted to investigate the relationship of C23 expression with the radiosensitivity of human non-small cell lung cancer (NSCLC) cells. We investigated the role of C23 in activating the catalytic subunit of DNA-dependent protein kinase (DNA-PKcs), which is a critical protein for DNA double-strand breaks (DSBs) repair. As a result, we found that the expression of C23 was negatively correlated with the radiosensitivity of NSCLC cell lines. In vitro clonogenic survival assays revealed that C23 knockdown increased the radiosensitivity of a human lung adenocarcinoma cell line, potentially through the promotion of radiation-induced apoptosis and adjusting the cell cycle to a more radiosensitive stage. Immunofluorescence data revealed an increasing quantity of ${gamma}$-H2AX foci and decreasing radiation-induced DNA damage repair following knockdown of C23. To further clarify the mechanism of C23 in DNA DSBs repair, we detected the expression of DNA-PKcs and C23 proteins in NSCLC cell lines. C23 might participate in DNA DSBs repair for the reason that the expression of DNA-PKcs decreased at 30, 60, 120 and 360 minutes after irradiation in C23 knockdown cells. Especially, the activity of DNA-PKcs phosphorylation sites at the S2056 and T2609 was significantly suppressed. Therefore we concluded that C23 knockdown can inhibit DNA-PKcs phosphorylation activity at the S2056 and T2609 sites, thus reducing the radiation damage repair and increasing the radiosensitivity of NSCLC cells. Taken together, the inhibition of C23 expression was shown to increase the radiosensitivity of NSCLC cells, as implied by the relevance to the notably decreased DNA-PKcs phosphorylation activity at the S2056 and T2609 clusters. Further research on targeted C23 treatment may promote effectiveness of radiotherapy and provide new targets for NSCLC patients.

• Korean journal of applied entomology
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• v.37 no.2
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• pp.159-162
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• 1998

Effect of temperature on oviposition and developmental period of Oulema oryzae K. on riceplant were investigated. With the given set of temperatures of 15, 20, 23, 25, and 28"C, developmentalperiod from egg to adult emergence was shorten as temperature increased. Average number of eggs perfemale 0. oryzae increased as temperature increased from 15$^{\circ}$C to 23"C, then decreased at 25$^{\circ}$C and 28"C. Based on this result, developmental threshold temperatures for egg was estimated to be 6.4"C. Totaleffective temperatures above the thresolds required for hatching were estimated to be 75.8 degree-daysfor egg. It seemed that the optimum temperature for oviposition of Oulema oryzae on rice plant was 23"C.a oryzae on rice plant was 23"C.quot;C.

• Journal of Bio-Environment Control
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• v.4 no.2
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• pp.144-151
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• 1995

This experiment was carried out to investigate the effect of different nutrient solution temperatures on the growth and quality of welsh onion in winter season. The fresh weight increased with the increase of temperature within the range of 13-23$^{\circ}C$. The top length was longer 18 and 23$^{\circ}C$ than at 13$^{\circ}C$, the dry weight was the highest at 18$^{\circ}C$. The potassium content in plant was the highest at 18$^{\circ}C$, calcium and magnesium contents were higher at 13 and 18$^{\circ}C$ than at 23$^{\circ}C$. The vitamin C content decreased with the increase of nutrient solution temperature, but there was no statistical significance within treatments. The pyruvic acid was higher at 13 and 18$^{\circ}C$ than at 23$^{\circ}C$, while nitrate content was lower at 13 and 18$^{\circ}C$ than 23$^{\circ}C$. Therefore, for growth and quality of welsh onion, it was concluded that 18$^{\circ}C$ may be suitable nutrient solution temperature in winter season.

• Journal of Mushroom
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• v.1 no.1
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• pp.34-43
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• 2003

This study was carried out to determine the proper pin-heading induction time(spawn running time) when different incubation temperature were applied to Pleurotus ostreatus(Chunchu 2ho, Suhan 1ho, Heukpyung). Incubation period was 17 days at 23 and 21 days at 17 for Chunchu 2ho, 17 days at 23 and 26 for Heukpyung, and 22~23 days at 17~23 for Suhan 1ho. Incubation period for Suhan 1ho was not significantly affected by incubation temperature. The time required for initial pin-heading was 4~5 days at 17, 20, 23 and 26 for Chunchu 2ho as well as Heukpyung, and was 3 days at 17, 20, 23 and 5~6 days at 26 for Suhan 1ho. As the incubation period became longer, the available fruit-bodies at Chunchu 2ho were made more but they were short. The yield of Chunchu 2ho and Heukpyung increased when incubated for 22~27 days at 20~23 and that of Suhan 1ho also increased when incubated for 22~23 days at 17~23.

• Korean Journal of Food Science and Technology
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• v.7 no.2
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• pp.74-81
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• 1975

Nonvolatile organic acids were determined in raw Chinese cabbage, Kimchis fermented at $6^{\circ}{\sim}7^{\circ}C$ and at $22^{\circ}{\sim}23^{\circ}C$. Total acids were isolated by ion-exchange chromatography and quantitative determination of individual acids was performed by silicic acid partition column chromatography. Acids were identified qualitatively by paper chromatography. Palatability of Kimchis was evaluated by nine pannels. Results were as follows: Maleic, fumaric, lactic, succinic, malonic, oxalic, glycolic, malic, citric, tartaric, sulphuric and phosphoric acid were found in raw Chinese cabbage. Kimchis fermented at $6^{\circ}{\sim}7^{\circ}C$, and at $22^{\circ}{\sim}23^{\circ}C$. In raw Chinese cabbage, most of organic acids were in salt form and malic acid was highest in amount. In Kimchi fermented at $6^{\circ}{\sim}7^{\circ}C$, the amount of lactic acid and succinic acid was higher than that in Kimchi fermented at $22^{\circ}{\sim}23^{\circ}C$, and, tartaric, oxalic, malic, malonic, maleic, glycolic and fumaric acids were less than those in Kimchi fermented at $22^{\circ}{\sim}23^{\circ}C$. Kimchi fermented at $6^{\circ}{\sim}7^{\circ}C$ had a more flavorous sour taste than Kimchi fermented at $22^{\circ}{\sim}23^{\circ}C$. It is assumed that large amount of lactic acid and succinic acid in Kimchi fermented st $6^{\circ}{\sim}7^{\circ}C$ have a connection with flavorous sour taste.

• Korean journal of applied entomology
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• v.36 no.4
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• pp.311-316
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• 1997

This study was conducted to evaluate the effect of temperatures on the egg and nymphal development andadult longevity of green mirid bug, Cvrtorhinus lividipennis Reuter. In addition, predation on eggs of brownplanthopper, Nilupurvurcz lugens ~t:l and small brown planthopper, L~orlelphux .striatellus Fallen by C.lividipennis were studied at different temperatures. Hatchability of C. lividipennis was about 96% at 23"C,88% at 26"C, 75% at 29$^{\circ}$C and 64% at 32$^{\circ}$C. Egg duration of C. lividipmnis was 1 1.0 days at 23"C, 10.0 at26"C, 6.7 days at 29$^{\circ}$C and 5.6 days at 32$^{\circ}$C. Nymphal duration of C. 1i1~idiperzni.ws as 13.7 days at 23"C,12.7 days at 26$^{\circ}$C. 10.2 days at 29$^{\circ}$C and 9.1 days at 32$^{\circ}$C regardless of food sources. Nymphaldevelopment was the shortest at 4th instar and the longest at 1st instar irrespective of temperatures and foodsources. Adult longevity of C. livirlipennis was was about 22.0 to 23.5 days at 23$^{\circ}$C. 19.0 to 20.0 days at26"C, 16.0 to 17.0 days at 2Y0C, and 1 1.0 to 12.0 days at 32$^{\circ}$C. There was no significant difference in adultlongevities on food sources. Number of eggs comsumed by adult C. lividipennis were about 56 to 61 and 56to 57,56 to 60 and 47 to 49,43 to 46 and 40 to 42, and 28 to 30 and 26 to 27 at 23'C. 20$^{\circ}$C. 29$^{\circ}$C and 32"C,respectively. Egg consumption by adult C. lividiprrznis was slightly higher at female and on N. lugens eggthan at male and on L. striatellus egg. Dail 2.0 to 3.0 eggs were consumed by adult C. lividipc,nni.s.ail 2.0 to 3.0 eggs were consumed by adult C. lividipc,nni.s.

• Natural Product Sciences
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• v.14 no.3
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• pp.152-155
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• 2008

An HPLC-ESI-MS method has been developed to identify and quantify two main tetracyclic triterpenes, alisol B 23-acetate and alisol C 23-acetate in the Alismatis Rhizoma (Taeg-Sa). The relative distribution of the two triterpenes in the methanolic extract of commercially available Alismatis Rhizoma was established by selective ion monitoring (SIM) mode via electrospray ionization (ESI) source. Regression equations revealed good linear relationship, and the correlation coefficients were 0.999 and 0.998 for alisol B 23-acetate and alisol C 23-acetate, respectively, between the peak areas of the components and their concentration in a range of $0.06-2.0{\mu}g/mL$. It was found that there were significant differences in the amount of alisol B 23-acetate and alisol C 23-acetate between Korean and Chinese origins. The results showed that this method could be used to identify the two components in Alismatis Rhizoma with high sensitivity and selectivity.

• Journal of Aquaculture
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• v.13 no.2
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• pp.107-117
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• 2000

Optima for temperature, salinity and light intensity for Nitzschia closterium, Chlorella salina, I내초교냔 galbana and Tetraselmis subcordiformis, which are widely used in bivalve hatcheries of Shandong Province in China, were estimated. The temperature optimum was 23 $^{\circ}C$ for N. closterium and I. galbana and 25 and 27$^{\circ}C$ for C. salina and T. subcordiformis, respectively. That for salinity was 23${\textperthousand}$ for N. closterium and T, subcordiformis, but was 33${\textperthousand}$ for C. salina and I. galbana. In general, all the four microalgae grew faster under 6,000 lux than under 4,000 lux. Growth of N. closterium was faster at $25^{\circ}C$ and dropped abruptly >$25^{\circ}C$, and that of C. salina and T. subcordiformis progressively increased upto $25^{\circ}C$ but dropped beyond 27$^{\circ}C$. T. subcordiformis was the most eurythermal among the 4 species. For mass culture of microalgae in Korea, N. closterium and C. salina are suitable during spring and autumn but C. salina and I. galbana during summer. T. subcordiformis is suitable for culture throughout the year.

• Korean Journal of Animal Reproduction
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• v.16 no.1
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• pp.63-76
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• 1992

This study was carried out to investigate the appearance time of the second polar body for producing Gynogenesis or Triploid which could be obtained by arresting the second polar body by cold shock, and then blastoderm was used to measure fertility that revealed the nature of oogenesis, the effects of water temperature on fertility, hatchability, abnormality, viability and growth rate, and the water temperature and the breeding methods to prevent early death of larvae in Korean loach(Misgurnus anguillicaudatus) ; the results obtained in this study were summarized as follows. The second polar body was observed ont he surface of plasma disc close to micropyle within 10~40 min after fertilization at 29$^{\circ}C$. Artificial inseminatin had to be done immediately after the egg spawning because the spermatozoa of loach their mobility within 2 minutes when they were exposed to water. The amount of time needed to reach at blastoderm stage was 12 hours if fertilized eggs were incubated at 16$^{\circ}C$, 8 hours at 19$^{\circ}C$, 6 hours at 21$^{\circ}C$, 5 hours at 23$^{\circ}C$, 4 hours at 26$^{\circ}C$ and 3 hours 30 min at 29$^{\circ}C$ showing the shorter time for development of eggs at higher temperature. Fertilization rates in water temperatures of 19$^{\circ}C$, 21$^{\circ}C$, 23$^{\circ}C$, and 26$^{\circ}C$ were higher than those of water temperatures, 16$^{\circ}C$ and 29$^{\circ}C$. Water temperatures at 19$^{\circ}C$, 21$^{\circ}C$, and 23$^{\circ}C$ showed higher hatching rates that those of 16$^{\circ}C$, 26$^{\circ}C$, and 29$^{\circ}C$, while abnormal rates in 16$^{\circ}C$, 19$^{\circ}C$, 21$^{\circ}C$ and 23$^{\circ}C$ were lower than that of 26$^{\circ}C$ and 29$^{\circ}C$. Water temperatures at 16$^{\circ}C$, 19$^{\circ}C$, 21$^{\circ}C$, 23$^{\circ}C$ and 26$^{\circ}C$ respectively, were more different than 29$^{\circ}C$ in survival rates. The embryos were hatched at 72 hours after fertilization in 16$^{\circ}C$ water temperature, 48 hours in 19$^{\circ}C$, 40 hours in 21$^{\circ}C$, 32 hours in 23$^{\circ}C$, 25 hours in 26$^{\circ}C$, and 16 hours in 29$^{\circ}C$. Within three days after hatched out, the larvage grew 3mm in total length, the yolk granules were entirely consumed and the head and the trunk became thicker. Within 45 days after hatched out, the larva grew 25mm at 29$^{\circ}C$, 21mm at 26$^{\circ}C$, 16mm at 23$^{\circ}C$, 15mm at 21$^{\circ}C$, 12mm at 16$^{\circ}C$ in a 30 litreglass aquarium.