• Journal of The Korean Society of Grassland and Forage Science
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• v.21 no.4
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• pp.253-258
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• 2001

This pot experiment was conducted to find out the forage productivity and quality in a grasslclover sward as affected by the application of three different fertilizers; double superphosphate(DS), fused Mg-phosphate (FP), and complex fertilizer(CF) on newly reclaimed hilly soil. This part was concerned with the mutual balances of mineral nutrients in the soils and mixed grass/clover sward in relation to grass tetany hazard. The results obtained are summarized as follows : 1. Concentration of exchangeable Mg and relative proportion of Mg to CEC in the soils before experiment were considerably below the critical level for good forage growth and prevention of grass tetany. It seems that these properties would be able to handicap by liming and NPK applications. 2. Comparing with the critical level for likelihood of tetany(Mg <0.2%, K >2.5%, and W(Ca+Mg) >2.2 in forages), mean concentration of Mg ranged from 0.14 in DS plot and 0.18 in FP plot to 0.24% in CF plot. Meanwhile, hazards of grass tetany in relation to the %K and ratio of K/(Ca+Mg) were not recognized. 3. Comparing with the optimum level of Carp(% ratio)=2.0 in forages for animal health, these ranged from 6.1 to 7.1. (Key words : Grass tetany, Fertilizer. Soil. Mineral nutrients)

• Journal of Environmental Health Sciences
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• v.22 no.2
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• pp.10-18
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• 1996

Experiments were carried out to find the possibility of an economic production of single cell protein(SCP) in mixed culture by Cellulomonas sp. KL-6 and a second organism. The second organism, strain LI-10, was isolated from the large intestines of a mouse. 1. When these strains were mixed, cell growth and carboxymethyl cellulase (CMCase) activity were increased to about 63% and 161%, respectively compared with that of single culture of strain KL-6. We found the mixed culture as a proper method of degradation of cellulose in our study. 2. Strain LI-10 was identified as E. coli. 3. This strain produced trace amounts of cellobiose, but glucose was not found in detectable amounts in the filter paper(FP) medium. 4. $CaCO_3$ injected in the medium at the ratio of 0.1% not only enhanced cell growth but also was effective as an acid neutralizing agent. 5. When this organism was cultured under the optimal medium (glucose 0.1%, $NH_4Cl$ 0.1%, yeast extract 2.0%, $KH_2PO_4$ 0.1%, KCl 0.05%, pH 7.2 and a temperature 30$\circ$C) for 5 days, a cell mass produced 1.18 g/l. The results showed the increase of cell mass up to 300% compared to 0.28 g/l produced in CMC medium.