• Journal of Veterinary Clinics
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• v.28 no.3
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• pp.307-309
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• 2011

A 1-year-old, intact female, Yorkshire terrier was referred with diarrhea and depression. Fecal examination indicated overgrowth of yeast-like organisms. CBC and blood smear revealed severe leukemoid reaction with degenerative changes of neutrophils. Radiographic examination showed decreased serosal detail and organomegaly, suggesting pyometra with peritonitis. Thus, the dog was suspected as pyometra with peritonitis. Culture and molecular analysis of the yeast-like organisms revealed that overgrown yeast was Candida parapsilosis.

• KSBB Journal
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• v.13 no.1
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• pp.6-12
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• 1998

In order to produce xylitol from hemicellulose hydrolysate which is widely used as a substrate, the development of strain such as catabolite derepressed mutant is required. After treatment of Candida sp. with EMS, GM-17 and PM-34 as catabolite derepressed mutant were isolated from Candida guilliermondii and Candida parapsilosis, respectively. Mutant GM-17 and PM-34 simultaneously assimilated xylose and glucose during the fermentation. The specific xylose reductase and xylitol dehydrogenase activities of mutant strains were also higher than those of wild strains in glucose medium and mixed medium of glucose and xylose. The xylitol productivity and yield of mutant GM-17 and PM-34 were improved as compared to the wild types in the mixed medium. The xylitol productivity and yield of mutant GM-17 were 0.09 g/L·hr and 0.56 g-xylitol/g-xylose, and those of mutant PM-34 were 0.21 g/L·hr and 0.52 g-xylitol/g-xylose in the mixed medium, respectively.

• Journal of Korean Neurosurgical Society
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• v.47 no.4
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• pp.295-297
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• 2010

Candida is a relatively rare cause of spinal infections that commonly affects immunocompromised patients. A 70-year-old woman, who underwent a lumbar discectomy on L5-S1 two months earlier, was admitted to our department complaining of persistent back and leg pain. Magnetic resonance imaging showed irregular enhancing mass lesion in L5-S1 intervertebral space, suggest of pyogenic discitis with epidural abscess. The surgery was performed via retroperitoneal approach and the infected material at L5-S1 intervertebral space was removed. The histological examination of the specimen revealed chronic inflammation involving the bone and soft tissue, and a culture of the excised material was positive for Candida parapsilosis. The patient received intravenous fluconazole for 4 weeks after surgery and oral fluconazole 400 mg/day for 3 months after surgery. The patient made a full recovery with no symptoms 6 months after surgery. We present a rare case of spondylodiscitis after a lumbar discectomy due to Candida parapsilosis and discuss treatment option with a review of the literatures.

• Journal of the Korean Chemical Society
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• v.55 no.2
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• pp.256-261
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• 2011

A new series of bis acetylated hybrid pyrazoles were synthesized and characterized by their melting point, elemental analysis, MS, FT-IR, one-dimensional $^1H$, and $^{13}C$ NMR spectroscopic data. All the synthesized compounds were tested for their in vitro antifungal activities against Candida sp. namely Candida albicans, Candida glabrata, Candida parapsilosis, Candida dubliniensis and Candida tropicalis. A close inspection of the in vitro anticandidal activity profile in differently electron donating ($CH_3$ and $OCH_3$) and electron withdrawing (-F, -Cl, and Br) functional group substituted phenyl rings of novel hybrid pyrazoles exerted strong anticandidal activity against all the tested Candida species.

• Journal of Microbiology and Biotechnology
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• v.19 no.1
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• pp.65-71
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• 2009

Microbial oxidoreductive systems have been widely used in asymmetric syntheses of optically active alcohols. However, when reused in multi-batch reaction, the catalytic efficiency and sustainability of non-growing cells usually decreased because of continuous consumption of required cofactors during the reaction process. A novel method for NADPH regeneration in cells was proposed by using pentose metabolism in microorganisms. Addition of D-xylose, L-arabinose, or D-ribose to the reaction significantly improved the conversion efficiency of deracemization of racemic 1-phenyl-1,2-ethanediol to (S)-isomer by Candida parapsilosis cells already used once, which afforded the product with high optical purity over 97%e.e. in high yield over 85% under an increased substrate concentration of 15 g/l. Compared with reactions without xylose, xylose added to multi-batch reactions had no influence on the activity of the enzyme catalyzing the key step in deracemization, but performed a promoting effect on the recovery of the metabolic activity of the non-growing cells with its consumption in each batch. The detection of activities of xylose reductase and xylitol dehydrogenase from cell-free extract of C. parapsilosis made xylose metabolism feasible in cells, and the depression of the pentose phosphate pathway inhibitor to this reaction further indicated that xylose facilitated the NADPH-required deracemization through the pentose phosphate pathway in C. parapsilosis. moreover, by investigating the cofactor pool, the xylose addition in reaction batches giving more NADPH, compared with those without xylose, suggested that the higher catalytic efficiency and sustainability of C. parapsilosis non-growing cells had resulted from xylose metabolism recycling NADPH for the deracemization.

• Microbiology and Biotechnology Letters
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• v.13 no.4
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• pp.371-376
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• 1985

We investigated the growth parameters of Candida parapsilosis isolated from spoiled red ginseng extract. The optimum pH range for C. parapsilosis was 6.0, whereas the minimum and maximum pH values that permitted growth were 3.0 and 11.0, respectively. For cells grown in PG medium plus 0 and 60％ sucrose, the optimum water activity(Aw) values were 0.98 and 0.97, respectively. The optimum temperature for C. parapsilosis were 3$0^{\circ}C$ at an Aw of 0.90 in 4.8％ potato dextrose broth with 18％ sucrose (PGS). Cations inhibiting the growth of C parapsilosis were L $i^{+}$ $Ca^{2+}$, $Mg^{+2}$, $K^{+}$ in decreasing order, while anions were S $O_4$$^{2-}$, N $O_3$$^{[-10]}$ , C $l^{[-10]}$ .TEX> .

• Journal of Mycology and Infection
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• v.23 no.4
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• pp.105-110
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• 2018

Background: Otomycosis is a fungal infection that comprises 7~10% of outer ear infections. Although the occurrence is higher in humid climates, relatively few studies have investigated otomycosis occurrences in humid environments. While recurrent chronic otitis media discharge in the ear creates a milieu in which otomycosis is likely to occur, investigations of otomycosis co-occurring with chronic otitis media have been rare. Objective: To examine the characteristics of patients with otomycosis co-occurring with chronic otitis media and identify causative fungi. Methods: The study included 60 patients with chronic otitis media who presented typical otomycosis findings in the outer ear canal and the presence of fungi. Patients were treated in the department of otolaryngology, Daegu Catholic University Medical Center, between July 2011 and June 2018. Results: The mean patient age was 57.77 years, and our study included 20 men and 40 women (p=0.010). The lesion was on the right in 39 patients and on the left in 21 (p=0.020). Ear discharge was the most common chief complaint at diagnosis. Of the 54 patients over age 19, 10 had diabetes (18.5%). Aspergillus was causative in 29 patients and Candida in 31. Aspergillus niger was identified in 15 patients, Aspergillus sp. in 14, Candida parapsilosis in 12, Candida sp. in six, and Candida albicans in five. Conclusion: Otomycosis and chronic otitis media co-occurrences increase with age. The Aspergillus and Candida genera were similar in proportion. A. niger was the most common Aspergillus species, while C. parapsilosis was the most common Candida.

• Journal of Ginseng Research
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• v.13 no.1
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• pp.66-70
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• 1989

The specific growth rate was investigated to find the of(emits of major ginseng components in fractions extracted by several solvents on the growth of Candida parapsilosis. The cell proliferation of C. purapsilosis was inhibited by fraction A(hexane soluble components), while enhanced by fraction B(Et2O soluble components), C(AcOEt soluble components), E and F(dialysis outer and inner extract). The additive stimulant effect of cell proliferation was exerted by fraction D(crude saponinE) and B, C or E.

• Microbiology and Biotechnology Letters
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• v.24 no.4
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• pp.507-511
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• 1996

Medium optimization for xylitol production from xylose by Candida parapsilosis ATCC 22019 mutant was performed. Effect of various nitrogen sources on xylitol production was investigatied. Of inorganic nitrogenous compounds, ammonium sulfate was effective for xylitol production and yeast extract was the most suitable orangic nitrogen nutrient for enhancement of xylitol production. Effect of inorganic salts such as KH$_{2}$PO$_{4}$ and MgSO$_{4}$-7H$_{2}$0 on xylitol production was also studied. Optimal medium was selected as xylose of 50 g/l, yeast extract of 5 g/l, (NH4$_{4}$)$_{2}$SO$_{4}$ of 5 g/l, KH$_{2}$PO$_{4}$ of 5 g/l, MgSO$_{4}$-7H$_{2}$O of 0.2 g/l. In a fermentor by using the optimal medium, a final xylitol concentration of 37 g/l could be obtained from 50 g/l of xylose with a xylitol yield of 74% and a xylitol productivity of 0.58 g/1-hr. At 300 g/l xylose, fermentation was also carried out and then a final xylitol concentration of 242 g/l was obtained at 272 hours. It was corresponding to xylitol yield of 80.7% and xylitol productivity of 0.58 g/1-hr.

• Korean Journal of Food Science and Technology
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• v.28 no.4
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• pp.756-761
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• 1996

Influence of acetic acid on xylitol production from xylose using Candida parapsilosis KFCC 10875 was investigated at the different concentrations of acetic acid. Acetic acid was totally consumed below 1.0 g/l of its concentration, whereas partially consumed above 3.0 g/l and remained in the medium during xylitol fermentation. Cell growth, xylose consumption, and xylitol production decreased when acetic acid concentration was increased. Specific growth rate of cell and specific consumption rate of xylose also decreased with increasing the concentration of acetic acid. However, the xylitol yield from xylose and specific production rate of xylitol were maximum at 1.0 g/l of acetic acid. The inhibitory effect of acetic acid on xylitol fermentation increased when pH was decreased.