• Journal of Biomedical Engineering Research
• /
• v.44 no.3
• /
• pp.176-181
• /
• 2023

During cancer treatment, the patient's response to drugs appears differently at the cellular level. In this paper, an image-based cell phenotypic feature quantification and key feature selection method are presented to predict the response of patient-derived cancer cells to a specific drug. In order to analyze the viability characteristics of cancer cells, high-definition microscope images in which cell nuclei are fluorescently stained are used, and individual-level cell analysis is performed. To this end, first, image stitching is performed for analysis of the same environment in units of the well plates, and uneven brightness due to the effects of illumination is adjusted based on the histogram. In order to automatically segment only the cell nucleus region, which is the region of interest, from the improved image, a superpixel-based segmentation technique is applied using the fluorescence expression level and morphological information. After extracting 242 types of features from the image through the segmented cell region information, only the features related to cell viability are selected through the ReliefF algorithm. The proposed method can be applied to cell image-based phenotypic screening to determine a patient's response to a drug.

• Proceedings of the Korean Society for Agricultural Machinery Conference
• /
• 2000.11b
• /
• pp.227-235
• /
• 2000

Agricultural products are easily deformable its shape because of some external forces. However, these force behavior is difficult to measure quantitatively. Until now, many researches on the mechanical property was performed with various methods such as material testing, chemical analysis and non-destructive methods. In order to investigate force behavior on the cellular unit of agricultural products, electro-microscope based 3D image processing method will contribute to analysis of plant cells behavior. Before image measurement of plant cells, plant sample was cut off cross-sectioned area in a size of almost 300-400 ${\mu}$ m units using the micron thickness device, and some of preprocessing procedure was performed with fixing and dyeing. However, the wall structure of plant cell is closely neighbor each other, it is necessary to separate its boundary pixel. Therefore, image merging and shrinking algorithm was adopted to avoid disconnection. After then, boundary pixel was traced through thinning algorithm. Each image from the electro-microscope has a information of x,y position and its height along the z axis cross sectioned image plane. 3D image was constructed using the continuous image combination. Major feature was acquired from a fault image and measured area, thickness of cell wall, shape and unit cell volume. The shape of plant cell was consist of multiple facet shape. Through this measured information, it is possible to construct for structure shape of unit plant cell. This micro unit image processing techniques will contribute to the filed of agricultural mechanical property and will use to construct unit cell model of each agricultural products and information of boundary will use for finite element analysis on unit cell image.

• KIPS Transactions on Software and Data Engineering
• /
• v.5 no.6
• /
• pp.267-272
• /
• 2016

Microscope cell image is an important indicator for obtaining the biological information in a bio-informatics fields. Since human observers have been examining the cell image with microscope, a lot of time and high concentration are required to analyze cell images. Furthermore, It is difficult for the human eye to quantify objectively features in cell images. In this study, we developed HCS algorithm for automatic analysis of cell image using an OpenCV library. HCS algorithm contains the cell image preprocessing, cell counting, cell cycle and mitotic index analysis algorithm. We used human cancer cell (MKN-28) obtained by the confocal laser microscope for image analysis. We compare the value of cell counting to imageJ and to a professional observer to evaluate our algorithm performance. The experimental results showed that the average accuracy of our algorithm is 99.7%.

• Journal of Korea Multimedia Society
• /
• v.18 no.10
• /
• pp.1180-1188
• /
• 2015

In this paper, a parallel cell contour line extraction algorithm using CUDA, which has no inner contour lines, is proposed. The contour of a cell is very important in a cell image analysis. It could be obtained by a conventional serial contour tracing algorithm or parallel morphology operation. However, the cell image has various damages in acquisition or dyeing process. They could be turn into several inner contours, which make a cell image analysis difficult. The proposed algorithm introduces a min-max coordinates table into each CUDA thread block, and removes the inner contour in parallel. It is 4.1 to 7.6 times faster than a conventional serial contour tracing algorithm.

• ETRI Journal
• /
• v.34 no.1
• /
• pp.138-141
• /
• 2012

This letter proposes a real-time digital image stabilization system for cell phone cameras without the need for frame memory. The system post-processes an image captured with a safe shutter speed using an adaptive denoising filter and a global color correction algorithm. This system can transfer the normal brightness of an image previewed under long exposure to the captured image making it bright and crisp with low noise. It is even possible to take photos in low-light conditions. By not needing frame memory, the approach is feasible for integration into the size-constrained image sensors of cell phone cameras.

• Proceedings of the Korean Society of Broadcast Engineers Conference
• /
• 2009.01a
• /
• pp.751-754
• /
• 2009

This paper deals with the Cell-based distributed processing for generating free viewpoint images by merging multiple differently focused images. We previously proposed the method of generating free viewpoint images without any depth estimation. However, it is not so easy to realize real-time image reconstruction based on our previous method. In this paper, we discuss the method to reduce the processing time by dimension reduction for image filtering and Cell-based distributed processing. Especially, the method of high-speed image reconstruction by the Cell processor on SONY PLAYSTATION3(PS3) is described in detail. We show some experimental results by using real images and we discuss the possibility of real-time free viewpoint image reconstruction.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
• /
• 2012.10a
• /
• pp.874-877
• /
• 2012

This paper proposes the new method based on contour following method with directional angle to segment the cell into the nuclei. The object image was the Thyroid Gland cell image that was diagnosed as normal and abnormal(two types of abnormal : follicular neoplastic cell, and papillary neoplastic cell), respectively. The nuclei were successfully diagnosed as normal and abnormal. this paper, improved method of digital image analysis required in basic medical science for diagnosis of cells was proposed. The object image was the Thyroid Gland cell image with difference of chromatin patterns. To segment the cell nucleus from background, the region segmentation algorithm by edge tracing was proposed. And feature parameter was obtained from discrete Fourier transformation of image. After construct a feature sample group of each cells, experiment of discrimination was executed with any verification cells. As a result of experiment using features proposed in this paper, get a better segmentation rate(70-90%) than previously reported papers, and this method give shape to get objectivity and fixed quantity in diagnosis of cells. The methods described in this paper be used immediately for discrimination of neoplastic cells.

• Korean Chemical Engineering Research
• /
• v.61 no.2
• /
• pp.247-257
• /
• 2023

Precise counting of cell number stands in important position within clinical and research laboratories. Conventional methods such as hemocytometer, migration/invasion assay, or automated cell counters have limited in analytical time, cost, and accuracy., which needs an alternative way with time-efficient in-situ approach to broaden the application avenue. Here, we present simple coding-based cell counting method using image analysis tool, freely available image software (ImageJ). Firstly, we encapsulated RFP-expressing bacteria in a droplet using microfluidic device and automatically performed fluorescence image-based analysis for the quantification of cell numbers. Also, time-lapse images were captured for tracking the change of cell numbers in a droplet containing different concentrations of antibiotics. This study confirms that our approach is approximately 15 times faster and provides more accurate number of cells in a droplet than the external analysis program method. We envision that it can be used to the development of high-throughput image-based cell counting analysis.

• Journal of information and communication convergence engineering
• /
• v.9 no.4
• /
• pp.466-470
• /
• 2011

This paper proposes the new method based on fuzzy logic which recognizes between normal and abnormal. The object image was the Thyroid Gland cell image that was diagnosed as normal and abnormal(two types of abnormal : follicular neoplastic cell, and papillary neoplastic cell), respectively. The nuclei were successfully diagnosed as normal and abnormal. The multiple feature parameters (pre-obtained 16 feature parameters of image data) were used to extract the features of each nucleus. As a consequence of using fuzzy logic algorithm, proposed in this paper, average recognition rate of 98.25% was obtained.

• Proceedings of the Korea Information Processing Society Conference
• /
• 2010.11a
• /
• pp.1118-1121
• /
• 2010

With the advent of cell phone digital cameras, and sophisticated photo editing software, digital images can be easily manipulated and altered. Although good forgeries may leave no visual clues of having been tampered with, they may, nevertheless, alter the underlying statistics of an image. Most digital camera equipped in cell phones employ a single image sensor in conjunction with a color filter array (CFA), and then interpolates the missing color samples to obtain a three channel color image. This interpolation introduces specific correlations which are likely to be destroyed when tampering with an image. We quantify the specific correlations introduced by CFA interpolation, and describe how these correlations, or lack thereof, can be automatically detected in any portion of an image. We show the efficacy of this approach in revealing traces of digital tampering in lossless and lossy compressed color images interpolated with several different CFA algorithms in test cell phones.