• Title/Summary/Keyword: Cell synchronization

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Effects of Cell Cycle Regulators on the Cell Cycle Synchronization of Porcine induced Pluripotent Stem Cells

  • Kwon, Dae-Jin;Hwang, In-Sul;Kwak, Tae-Uk;Yang, Hyeon;Park, Mi-Ryung;Ock, Sun-A;Oh, Keon Bong;Woo, Jae-Seok;Im, Gi-Sun;Hwang, Seongsoo
    • Development and Reproduction
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    • v.21 no.1
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    • pp.47-54
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    • 2017
  • Unlike mouse results, cloning efficiency of nuclear transfer from porcine induced pluripotent stem cells (piPSCs) is very low. The present study was performed to investigate the effect of cell cycle inhibitors on the cell cycle synchronization of piPSCs. piPSCs were generated using combination of six human transcriptional factors under stem cell culture condition. To examine the efficiency of cell cycle synchronization, piPSCs were cultured on a matrigel coated plate with stem cell media and they were treated with staurosporine (STA, 20 nM), daidzein (DAI, $100{\mu}M$), roscovitine (ROSC, $10{\mu}M$), or olomoucine (OLO, $200{\mu}M$) for 12 h. Flow Cytometry (FACs) data showed that piPSCs in control were in G1 ($37.5{\pm}0.2%$), S ($34.0{\pm}0.6%$) and G2/M ($28.5{\pm}0.4%$). The proportion of cells at G1 in DAI group was significantly higher than that in control, while STA, ROSC and OLO treatments could not block the cell cycle of piPSCs. Both of viability and apoptosis were affected by STA and ROSC treatment, but there were no significantly differences between control and DAI groups. Real-Time qPCR and FACs results revealed that DAI treatment did not affect the expression of pluripotent gene, Oct4. In case of OLO, it did not affect both of viability and apoptosis, but Oct4 expression was significantly decreased. Our results suggest that DAI could be used for synchronizing piPSCs at G1 stage and has any deleterious effect on survival and pluripotency sustaining of piPSCs.

A Study on Partial Correlation based PSS Detection Schemes for Initial Cell Search in LTE Systems (LTE 시스템에서 초기 셀 탐색을 위한 부분 상관값 기반 PSS 검출 방식에 관한 고찰)

  • Joo, Jung Suk
    • Journal of the Institute of Electronics and Information Engineers
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    • v.51 no.9
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    • pp.3-10
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    • 2014
  • The synchronization signal in Long Term Evolution (LTE) systems contains the primary synchronization signal (PSS) and the secondary synchronization signal (SSS), and the overall synchronization performance is dominated by PSS detection. In this paper, we propose a PSS detection scheme employing three partial correlation based PSS detectors with different partial correlation lengths, in which calculated partial correlation values are reused in order to reduce computational complexity. A coherent selection scheme using tentative frequency offsets is also proposed to select one reliable result from outputs of partial correlation based PSS detectors. It is shown through computer simulation that the proposed scheme can have a robust detection performance in wide range of frequency offsets.

A study on Generalized Synchronization in the State-Controlled Cellular Neural Network(SC-CNN)

  • Rae Youngchul;Kim Yi-gon;Tinduka Mathias
    • International Journal of Fuzzy Logic and Intelligent Systems
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    • v.5 no.4
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    • pp.291-296
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    • 2005
  • In this paper, we introduce a generalized synchronization method and secure communication in the State-Controlled Cellular Neural Network (SC-CNN). We make a SC-CNN using the n-double scroll. A SC-CNN is created by applying identical n-double scroll or non-identical n-double scroll and Chua's oscillator with weak coupled method to each cell. SC-CNN synchronization was achieved using GS(Generalized Synchronization) method between the transmitter and receiver about each state variable in the SC-CNN. In order to secure communication, we have synthesizing the desired information with a SC-CNN circuit by adding the information signal to the hyper-chaos signal using the SC-CNN in the transmitter. And then, transmitting the synthesized signal to the ideal channel, we confirm secure communication by separating the information signal and the SC-CNN signal in the receiver.

The Synchronization and Secure Communication of Hyper-chaos circuit using SC-CNN (SC-CNN을 이용한 하이퍼카오스 회로에서의 동기화 및 비밀 통신)

  • 배영철;김주완
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.6 no.7
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    • pp.1064-1068
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    • 2002
  • In this paper, we introduce a hyper-chaos synchronization method using State-Controlled Cellular Neural Network(SC-CNN). We make a hyper-chaos circuit using SC-CNN with the n-double scroll. A hyper-chaos circuit is created by applying identical n-double scrolls with weak coupled method, to each cell. Hyper-chaos synchronization was achieved using drive response synchronization between the transmitter and receiver about each state in the SC-CNN. From the result of the recovery signal through the demodulation method in the receiver, We shown that recovery quality of state variable $$\chi$_3$ is superior to that of ${$\chi$_2}, {$\chi$_1}$ in secure communication.

The Simplified PWM Method using Serial Communication in Cascaded H-Bridge Multilevel Inverter (직렬통신을 이용한 H-브릿지 멀티레벨 인버터의 PWM 구현방법)

  • Park Young-Min;Ryu Han-Seong;Lee Hyun-Won;Lee Se-Hyun;Lee Chung-Dong;Yoo Jl-Yoon
    • The Transactions of the Korean Institute of Power Electronics
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    • v.9 no.6
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    • pp.620-627
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    • 2004
  • As h-bridge multilevel inverter is connected with series of single phase power cell, so it obtain high voltage using low voltage power semi-conductor and output voltage similar to sine wave. In this topology, the number of power cell increases in proportion to the output voltage level. Therefore, there are drawbacks that are responsibility against operating ability of main controller and signal wire increase. However, we can overcome this problems by the substitution of serial communication for the PWM signal in power cell control. Additionally, it has merits of reliability and maintenance. This paper deals with the synchronization and phase-shift method of power cell PWM using CAN(Controller Area Network) communication interrupt in H-bridge multilevel inverter. The advantages of proposed method are signal-line simplification using serial communication between main controller and cell controller, burden reduction in main controller, modularization of power cell, easy protection of each power cell, expandability improvement and reliability increase of control signal and power cell. This paper establishes propriety and reliability of proposed method through experiment of 13-level H-bridge multilevel inverter.

Design of a 3GPP LTE system Information(MIB) detection simulator (3GPP LTE 시스템정보(MIB) 추출에 대한 연구)

  • Gwag, Gyoung-Hun;Oh, Hyuk-Jun
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2015.10a
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    • pp.827-829
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    • 2015
  • This paper presents process of the UE obtains the cell system information in order to achieve communication with the LTE network on the LTE. This paper deals with the process of extracting MIB(Master Information Block) cell system information in LTE. To study the 3GPP LTE standard spec series 36.200 and 36.300, the simulation is implemented to extract the MIB in MATLAB program. The simulation process is divided into three parts. It consisted of a part that extracts the PSS, SSS and MIB. Called a cell search process for detecting the PSS and SSS, the process is to be done in order to extract only the cell system information. The simulation program is verified based on the signal captured from the real Air.

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A Design of Petri net-based AIM Supervisory Control System (페트리네트 기반 AIM 관리 제어 시스템의 설계)

  • Kong S.H.;Kim H.R.;Suh I.H.
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2005.06a
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    • pp.203-206
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    • 2005
  • This paper presents a design experience of supervisory control system for agile and intelligent manufacturing(AIM). For effectively program job instructions, a Petri net-type graphical language is proposed and it can be applied to a various task such as concurrency and synchronization. PGL is consisted of PGL editor, PGL analyzer and PGL translator; PGL editor generates a job instruction program using graphic symbol. PGL analyzer prevents a deadlock or resource allocation of unit cell. PGL translator transfers to adequate sequential job commands of each unit cell.

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The secure communication in hyper-Chaos

  • Youngchul Bae;Kim, Juwan;Kim, Yigon
    • Proceedings of the Korean Institute of Intelligent Systems Conference
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    • 2003.09a
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    • pp.575-578
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    • 2003
  • In this paper, we introduce a hyper-chaos secure communication method using Hyper-chaos consist of State-Controlled Cellular Neural Network (SC-CNN). A hyper-chaos circuit is created by applying identical n-double scroll with weak coupled method to each cell. Hyper-chaos synchronization was achieved using embedding synchronization between the transmitter and receiver about in SC CNN. And then, we accomplish secure communication by synthesizing the desired information with a hyper-chaos circuit by embedding the information signal to the only one state variable instead of all state variables in the driven-synchronization method. After transmitting the synthesized signal to the identical channel, we confirm secure communication by separating the information signal and the hyper-chaos signal in the receiver.

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A Nonlinear Synchronization Scheme for Hindmarsh-Rose Models

  • Kim, Jung-Su;Allgower, Frank
    • Journal of Electrical Engineering and Technology
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    • v.5 no.1
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    • pp.163-170
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    • 2010
  • Multiple subsystems are required to behave synchronously or cooperatively in many areas. For example, synchronous behaviors are common in networks of (electro-) mechanical systems, cell biology, coupled neurons, and cooperating robots. This paper presents a feedback scheme for synchronization between Hindmarsh-Rose models which have polynomial vector fields. We show that the problem is equivalent to finding an asymptotically stabilizing control for error dynamics which is also a polynomial system. Then, an extension to a nonlinear observer-based scheme is presented, which reduces the amount of information exchange between models.

The Secure Communication using Complexity (복잡계를 이용한 비밀 통신)

  • 배영철
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.8 no.2
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    • pp.365-370
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    • 2004
  • In this paper, complexity secure communication was presented. The complexity circuit is used to State-Controlled Cellular Neural Network(SC-CNN). We make a complexity circuit using SC-CNN with the N-double scroll. A complexity circuit is created by applying identical n-double scrolls with coupled method, to each cell. complexity synchronization was achieved using drive response synchronization between the transmitter and receiver about each state in the SC-CNN. From the result of the recovery signal through the demodulation method in the receiver. We shown that recovery quality in the receiver is the similar to other secure communication methods.