• Title, Summary, Keyword: Chrysanthemum boreale M.

Search Result 28, Processing Time 0.029 seconds

Antibacterial Activities of Extracts from Chrysanthemum boreale M. (산국 추출물의 항균력)

  • Yang, Min-Suk;Nam, Sang-Hae
    • Applied Biological Chemistry
    • /
    • v.38 no.3
    • /
    • pp.269-272
    • /
    • 1995
  • Antibacterial activity test of solvent fractions, sesquiterpenoid lactones, and Compound I and II extracted from Chrysanthemum boreale M. and Chrysanthemum indium L. were performed against four microorganisms. Among the tested substances, antibacterial activities were appeared against B. subtilis and V. parahaemolyticus at the chloroform fraction, sesquiterpenoid lactones and Compound I extracted from C. boreale. But chloroform fraction and sesquiterpenoid lactones extracted from C. indicum were showed weakly than those of C. boreale. Compound II and all fractions extracted from C. indicum were not appeared against the all tested microorganisms.

  • PDF

Structural Analysis and Biological Activities of Sesquiterpene Lactones Isolated from the Leaves and Stems of Chrysanthemum boreale Makino (산국의 잎과 줄기에서 분리한 Sesquiterpene Lactone들의 구조규명 및 생리활성)

  • Lee, Jong Rok;Park, Moon Ki
    • Journal of Environmental Science International
    • /
    • v.26 no.11
    • /
    • pp.1285-1295
    • /
    • 2017
  • Chrysanthemum boreale Makino is widely distributed in Korea, China, Japan and Southeast Asian countries. C. boreale is one of the herbs used for treating various inflammatory diseases in oriental medicine. The present study was conducted to identify biologically active compounds from the leaves and stems of C. boreale. We isolated two sesquiterpene sactones from the leaves and stems of C. boreale using silica gel column chromatography and recyclic high perfomance liquid chromatography. The lactones were characterized by their spectroscopic data (NMR, IR, MASS). These compounds were subjected to Farnesyl Protein Transferase (FPTase) inhibition, Nitric Oxide (NO) release inhibition and apoptosis inhibition. The structur of the following isolated compound were elucidated 8,10-${\small{O}$-Acetyl-2-methoxy-10-hydroxy-3,11(13)-guaiadiene-12,6-olide and 4,10-dihydroxy-8-${\small{O}$-Acetyl-2,11(13)-guaiadiene-12,6-olide. In the NO release inhibition assay, compound 2 showed strong activities, with an $IC_{50}$ value of $7{\mu}g/mL$, whereas compound 1 did not exhibit significant activity with an $IC_{50}$ value of over $14{\mu}g/mL$ against murine macrophage.

Effects of Chrysanthemum boreale M. Water Extract on Serum Liver Enzyme Activities and Kupffer Cells of Carbon Tetrachloride-Induced Rats

  • Jeon, Jeong-Ryae;Park, Jyung-Rewng
    • Food Science and Biotechnology
    • /
    • v.14 no.2
    • /
    • pp.290-296
    • /
    • 2005
  • Effects of water extract obtained from Chrysanthemum boreale M. (CE) on serum enzyme activities and Kupffer cells of carbon tetrachloride ($CCl_4$)-induced rats were investigated. Thirty-two healthy male Sprague-Dawley rats were divided into normal (N), $CCl_4$-induced (T), CE-supplemented (C), and $CCl_4$-induced and CE-supplemented (TC) groups. $CCl_4$ injection significantly increased aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, and alkaline phosphatase activities in serum. Significant increases in total cholesterol and triglyceride concentrations were also observed in $CCl_4$-induced rats. Oral administration of CE at 300 mg/kg body weight significantly decreased serum enzyme levels and suppressed $CCl_4$ hepatotoxicity-induced lipid profile changes. Histological findings showed fatty change, fibrosis and increased number of Kupffer cells in T group. Electron microscopic examination showed increased lysosome content and dilation of rough endoplasmic reticulum within Kupffer cells in T group, whereas CE supplement attenuated liver injury in $CCl_4$-induced liver. These results indicated CE could significantly alleviate CC4-induced hepatotoxicity injury.

Isolation and Characterization of Constituent Compounds from Leaves and Stems of Chrysanthemum boreale Makino (산국 잎과 줄기의 유효성분 분리 및 특성 연구)

  • Park, Sook Jahr;Park, Moon Ki;Lee, Jong Rok
    • Journal of Environmental Science International
    • /
    • v.28 no.11
    • /
    • pp.993-1004
    • /
    • 2019
  • Chrysanthemum boreale Makino (C. boreale) is widely distributed in Asian countries, and has traditionally been used to treat various inflammatory diseases including bronchitis. In this study, we aimed to isolate biologically active compounds from leaves and stems of C. boreale. Chemical components were purified by column chromatograpy and recyclic HPLC, and characterized from their spectral data (IR, MS, NMR). Biological activity experiments were conducted for Farnesyl-protein transferase (FPTase) activity, apoptosis and nitirc oxide (NO) release. As a results, three sesquiterpene lactones were isolated. Compound 1 (4-methoxy-8-O-acetyl-10-hydroxy-2,11(13)-guaiadiene-12,6-olide) showed strong cytotoxic activities having an average growth inhibition of 50% ($GI_{50}$) value of $1.89{\mu}g/m{\ell}$ against human colon adenocarcinoma cells. Compound 1 also showed a low half maximal inhibitory concentration ($IC_{50}$) value of $10{\mu}g/m{\ell}$ for NO release. In the caspase 3 activity, compound 1 and compound 2 (8-O-(2-carbonyl-2-butyl)-3,10-dihydroxy-4,11(13) -guaiadiene-12,6-olide) exhibited 94% and 90% apoptosis inhibition activity, respectively. Compound 3 (4,8-O-diacetyl -10-hydroxy-2(3),11(13)-guaiadiene-12,6-olide) showed a strong inhibitory effect on FPTase activity with 90% inhibitory activity at a concentration of $100{\mu}g/m{\ell}$. These results clearly show the presence of lactone compounds in the leaves and stems, which may partially contribute to the pharmacological activity of C. boreale.

Induction of Apoptosis in Human Oral Epidermoid Carcinoma Cells by Essential Oil of Chrysanthemum boreale Makino

  • Cha, Jeong-Dan;Jeong, Mi-Ran;Lee, Young-Eun
    • Food Science and Biotechnology
    • /
    • v.14 no.3
    • /
    • pp.350-354
    • /
    • 2005
  • The effect of the essential oil obtained from Chrysanthemum boreale Makino on the apoptosis of KB cells was investigated. Cytotoxicity and cellular DNA content were analyzed by MTT assay, flow cytometry, agarose gel electrophoresis, and Hoechst 33258 staining. The caspase-3 and poly (ADP-ribose) polymerase (PARP) proteins were estimated by Western blotting method. The various cytotoxic effects of the essential oil which are hallmarks of apoptosis, including DNA fragmentation, apoptotic body formation, and sub-G1 DNA content, all progressed in a dose-dependent manner. Treatment with an apoptosis-inducing concentration of the essential oil caused rapid and transient induction of caspase 3 activity. Further, the efficacious induction of PARP cleavage and caspase-3 activation was observed at an essential oil concentration of 0.1 and 0.2 mg/mL for 12 hr.

Anti-cariogenic Properties of α-Pinene, a Monoterpene in Plant Essential Oil

  • Park, Bog-Im;You, Yong-Ouk;Mo, Ji-Su;An, So-Youn;Choi, Na-Young;Kim, Kang-Ju
    • International Journal of Oral Biology
    • /
    • v.42 no.1
    • /
    • pp.25-31
    • /
    • 2017
  • Dental caries is the most common chronic disease in the dental field. Streptococcus mutans (S. mutans) is the most important bacteria in the formation of dental plaque and dental caries. In a previous study, we confirmed that the essential oil of Chrysanthemum boreale has antibacterial activity against S. mutans. Alpha-pinene is one of the major chemical components of Chrysanthemum boreale essential oil. In the present study, we investigated the inhibitory effects of ${\alpha}-pinene$ on cariogenic properties such as growth, acid production, biofilm formation, and bactericidal activity on S. mutans. Alpha-pinene at a concentration range of 0.25-0.5 mg/mL significantly inhibited the growth of S. mutans and acid production of S. mutans. Biofilm formation was significantly inhibited at > 0.0625 mg/mL ${\alpha}-pinene$, similar to the data from scanning electronic microscopy. Under confocal laser scanning microscopy, the bacterial viability was decreased by ${\alpha}-pinene$ in a dose-dependent manner. These results suggested that ${\alpha}-pinene$ may be a useful agent for inhibiting the cariogenic properties of S. mutans.

Quality Characteristics and Antioxidant Activity of Chrysanthemum indicum L., Chrysanthemum boreale M. and Chrysanthemum zawadskii K. Powdered Teas (감국, 산국 및 구절초꽃 분말 차의 항산화활성과 품질특성)

  • Lee, Sang-Hoon;Hwang, In-Guk;Nho, Jin-Woo;Chang, Young-Deug;Lee, Chul-Hee;Woo, Koan-Sik;Jeong, Heon-Sang
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.38 no.7
    • /
    • pp.824-831
    • /
    • 2009
  • In order to investigate the quality characteristics of powdered teas using Compositae species flower, the samples of Chrysanthemum indicum L. (CI), Chrysanthemum boreale Makino (CB), and Chrysanthemum zawadskii var. latilobum (Maxim.) Kitam (CZ) were extracted with hot water and concentrated, and then mixed with different forming agents of dextrin (D) and maltodextrin (MD). The mixing ratio of D and MD was ranged from 9:1 (DMD91) to 8:2 (DMD82) or D and MD only. The solubility of the powdered tea was higher in added dextrin. The highest total polyphenol and flavonoid content of the powdered tea were 6.75 and 3.24 mg/g at CBDMD91, respectively. Total antioxidant activities of C. indicum, C. boreale, and C. zawadskii powdered tea ranged at $2.51{\sim}2.63$, $4.37{\sim}4.50$, and $3.44{\sim}3.55\;mg$ AA eq/g, respectively. In sensory evaluation, the C. indicum and C. zawadskii powdered teas obtained higher sensory score of all evaluation items than C. boreale powdered tea. The optimal mixing ratio at 36% of D and 4% of MD in forming agent was selected to enhance the sensory characteristics and antioxidant activities of powdered tea.

Effects of Nitrogen Fertilization on the Yield and Effective Components of Chrysanthemum boreale M. (질소시비가 산국의 수량과 유효성분에 미치는 영향)

  • Lee, Kyung-Dong;Yang, Min-Suk;Lee, Young-Bok;Kim, Pil-Joo
    • Korean Journal of Soil Science and Fertilizer
    • /
    • v.35 no.1
    • /
    • pp.38-46
    • /
    • 2002
  • Chrysanthemum boreale M. (hereafter, C. boreale M.), a perennial flower, has been historically used as a natural medicine in Korea. With increasing concerns for health-improving foods, the demand for C. boreale M. has become higher than ever. Howevr, the amount of wild C. boreale M. collected from mountainous areas is not enough to cover all demands. The cultivation system and fertilization strategy are required to meet increasing demand on C. boreale M. with a good quality. We investigated the effects of nitrogen application on plant growth and effective components of C. boreale M. to suggest optimum rate of nitrogen fertilization. C. boreale M. was cultivated in a pot scale (1/2000a scale), and nitrogen applied with rate of 0(N0), 50(N50), 100(N100), 150(N150), 200(N200), and $250(N250)kg\;ha^{-1}$. Phosphate and potassium were applied at the same level ($P_2O_5-K_2O=80-80kg\;ha^{-1}$) in all treatments. Maximum yield achieved in 246 and $226kg\;ha^{-1}$ N treatment on the whole plant and the flower part, a valuable part as a herbal medicine, respectively. Proline was the most abundant amino acid in the flower of C boreal M. and the contents of amino acids increased with increasing nitrogen application rate in flower. Nitrogen recovery efficiency was high more than 41% in all nitrogen treatments and increased to 61.8% in nitrogen N100 treatment. From the nitrogen content, the high nitrogen uptake, the low residue of mineral N and the reasonably good apparent fertilizer recovery, it can be inferred that C. boreale M. made efficient use of the available nitrogen. In flower, contents of Cumambrin A. which is a sesquiterpene compound and has the effect of blood-pressure reduction, decreased with increasing nitrogen application. However, the amount of Cumambrin A in flower increased as nitrogen rate increased, because of increasing flower yield. Conclusively, nitrogen fertilization could increase yields and enhance quality. The optimum nitrogen application rate might be on the range of $225{\sim}250kg\;ha^{-1}$ in a mountainous soil.

Effect of ABA and Salicylic Acid on Yields, Mineral Contents and Active Components of Chrysanthemum boreale M. (산국의 수량, 무기물 및 유효성분에 미치는 ABA와 SA의 효과)

  • Lee, Kyung-Dong;Tawaha, Abdel R.M.;Yang, Min-Suk
    • Korean Journal of Medicinal Crop Science
    • /
    • v.12 no.6
    • /
    • pp.508-514
    • /
    • 2004
  • Chrysanthemum boreale M. is an important medicinal plant that has been historically used in herbal medicine and in the health food throughout East Asia. This study was conducted to investigate the influence of abscisic acid (ABA) and salicylic acid (SA) on plant growth, mineral content and effective components, such as essential oil, amino acid and cumambrin A, by means in order to increase the productivity and the quality of flowerheads in the plant. Yields of flowerheads were increased by 12.7%, 21.7% and 15.5% by ABA, SA and both treatments, respectively, as compared with the control. Inorganic nutrient content was changed by PGRs; SA treatment was increased by nitrogen, phosphorus and magnesium content but decreased by potassium of C. boreale M. flowerheads. Total content of amino acid was increased by SA but decreased by ABA treatment. Essential oil content and yields were increased to 9.7% and 33.8% by SA treatment. Moreover, the content of terpene, monoterpenoids and sesquiterpenoids, were improved by ABA treatment, especially, germacrene-D content was increased by 39.1%, as compared to control. In addition, yields of cumambrin A, sesquiterpene compound exhibiting blood-pressure activity, increased in all PGRs treatments, but its concentration in the C. boreale M. flowerheads only increased by ABA and both treatment. The experiment suggests that PGRs using ABA and SA could increase the yields and quality of C. boreale M. flowerheads.