• Title, Summary, Keyword: Chrysanthemum boreale M.

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Islation of Aldose Reductase Inhibitors from the Flowers of Chrysanthemum boreale

  • Shin, Kuk-Hyun;Kang, Sam-Sik;Seo, Eun-Ah;Shin, Seung-Won
    • Archives of Pharmacal Research
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    • v.18 no.2
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    • pp.65-68
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    • 1995
  • The methanol extract from the whole parts of the flowers of Chrysanthemum boreals was found to exhibit a significant inhibition of a rat lens aldose reductase (RLAR) activity in vitro. Bioassay guided systematic fractionation of the methanol extract led to the isolation of four flavonoids which were identified as acacetin (1), apigenin (II), luteolin (III) and linarin (IV). Compounds I-III were demonstrated to exhibit a significant inhibition of RLAR. Luteolin (II) was found to be the most potent AR inhibitor with $IC_{50}$ value of $5{\times}10^{-7}M$.

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Effects of Pig Manure Application on Nitrogen Uptake, Yield and Active Components of Chrysanthemum boreale M. (돈분퇴비 시용이 산국의 질소흡수 및 수량과 유호성분에 미치는 영향)

  • Lee, Kyung-Dong;Yang, Min-Suk
    • Korean Journal of Medicinal Crop Science
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    • v.11 no.5
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    • pp.371-376
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    • 2003
  • To develop an efficient cultivation system to increase the productivity and the high quality of Chrysanthemum boreale M., the effects of pig manure (PM) application on the yield and the effective component were investigated in the pot scale (1/2000a scale). PM applied at the equivalent of six rates (with rate of 0, 2000, 4000, 6000, 8000, and 12000 kg $10a^{-1}$). Maximum plant biomass yield was achieved at 9510 kg $10a^{-1}$ and at 9940 kg $10a^{-1}$ for flower biomass. Nitrogen recovery efficiency was more than 42% for all nitrogen treatments and reached 66.6% at 4000 kg $10a^{-1}$. Proline $(7.4{\sim}9.2\;g\;kg^{-1})$ was the most abundant amino acid in the flower of C. boreale M. and the contents of amino acids increased with increasing PM application rate. Contents of cumambrin A. a sesquiterpene compound known to reduce blood-pressure, decreased with increasing PM application. The highly negative correlation was found ($R^2$ = -0.723, P<0.01) between content of cumambrin A and PM application. However, the amount of cumambrin A in flowers increased as PM rate increased, because of increasing flower yield. In conclusion, PM fertilization increases yields and enhances overall quality.

Effect of Soil Amendment Application on Yields and Effective Components of Chrysanthemum boreale M. (산국의 수량과 유효성분에 대한 토양개량제의 효과)

  • Lee, Kyung-Dong;Lee, Yong-Bok;Yang, Min-Suk;Kim, Pil-Joo
    • Korean Journal of Soil Science and Fertilizer
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    • v.35 no.1
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    • pp.27-37
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    • 2002
  • With increasing the concerns of health improving foods. the demands of C. boreale M., which is a perennial flower and has been historically used for a natural medicine, become higher, recently, However, wild C. boreale M. collected in a mountatinous area is limited and not enough to cover all demands. The cultivation system and fertilization strategy are required to produce much amount of C. boreale M. with a good quality. We investigated the effects of soil amendment application on plant growth and effective components of C. boreale M. to develop efficient cultivation system. C. boreale M. was cultivated in a pot scale, and lime, fly ash, poultry manure compost and swine manure compost as an amendment applied with rate of 2, 20, 150 and $150Mg\;ha^{-1}$, respectively. Here, chemical fertilizers were applied with the same level ($N-P_2O_5-K_2O=150-80-80kg\;ha^{-1}$) in all treatments. Flower yields of C. boreale M., edible part as a natural medicine, were increased to 37 and 27% by swine and poultry manure compost application, respectively. Poultry manure compost amending (NPK+PMC) increased 3.6 times of proline content and 58% of total amino acids in the flower part more than chemical fertilization (NPK). But the contents of amino acids did not increase with amending liming materials like lime and fly ash. Cumambrin A, which is a sesquiterpene compound and has the effect of blood-pressure reduction, increased to 34 and 19% by lime and fly ash applications, respectively. Cumambrin A was significantly correlated with calcium content in the flower part of C. boreale M. Conclusively, soil amendments like compost and liming materials might contribute to increase the yields and quality of C. boreale M.

Effect of Hot-air Drying Temperature on Volatile Compounds in Chrysanthemum boreale M. Flowers (열풍 건조온도에 따른 산국의 휘발성 성분 변화)

  • Bae, Sung-Mun;Lee, Seung-Cheol
    • Korean Journal of Food Science and Technology
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    • v.40 no.4
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    • pp.466-469
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    • 2008
  • This study examined the effect of drying temperature on the qualitative properties of Chrysanthemum boreale M. flowers. The flower samples were dried in a hot air dryer at $40^{\circ}C$, $50^{\circ}C$, and $60^{\circ}C$, respectively, to attain a $23{\pm}1%$ moisture content. The time required to reach the target moisture content was 8.5-69 hr, and there was a very high negative correlation between log (spending time) and temperature. The Hunter color L- and b-values of the flowers were decreased with increasing drying temperature, whereas the a-value was increased. The volatile compounds contained in the dried flowers were determined by a solid-phase microextraction method. Twelve primary volatile compounds were detected and then quantified based on the GC chromatograms of the samples. The total contents of volatile compounds were increased with increasing drying temperature, and germacrene D and camphor were the main compounds in all samples.

Effect of NPK Fertilization on the Yields and Effective Components of Chrysanthemum boreale M. (산국의 수량과 유효성분에 대한 NPK의 효과)

  • Yang, Min-Suk;Jung, Yeun-Kyu;Sohn, Bo-Kyoon;Cho, Ju-Sik;Lee, Seong-Tae;Kim, Pil-Joo;Lee, Kyung-Dong
    • Applied Biological Chemistry
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    • v.46 no.2
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    • pp.134-139
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    • 2003
  • To establish the fertilization condition to increase the productivity of Chasanthemum boreale M. with high quality, the effects of three nutrients (N, P, K) on the yields and the effective components were investigated in the pot scale. NPK was applied by chemical fertilizers with $(N-P_2O_5,-K_2O=250-160-160\;kg/ha)$ as a main treatment, and NP $(N-P_2O_5,-K_2O=250-160-160\;kg/ha)$, NK $(N-K_2O =250-160\;kg/ha)$, and PK $(P_2O_5K_2O=160-160\;kg/ha)$ treatments were settled as comparison. Dry yields of C. boreale M. was increased significantly to 4 fold higher by nitrogen. Nitrogen increased apparently plant growth and inorganic nutrient uptake. In the flower, which is most useful and edible part as a herbal medicine, main amino acids were glutamic acid and aspartic acids, and the total content was increased significantly by three elements of application. In addition, the content of cumambrin A, which is known to have the effect of blood-pressure reduction, was increased source to 6.2 times by nitrogen higher than that in PK treatment. Potassium was more effective in biosynthesis of cumambrin A than phosphorus, but the biological pathway was not clear, still.

Isolation of Guaianolides with ACAT Inhibitory Activity from the Leaves and Stems of Chrysanthemum boreale Makino (산국의 잎과 줄기에서 ACAT 저해활성을 가지는 Guaianolides의 분리)

  • Lee, Jong Rok;Park, Moon Ki
    • Journal of Environmental Science International
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    • v.26 no.11
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    • pp.1275-1284
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    • 2017
  • Acyltransferase (AT) catalyzes the transfer of an acyl moiety from acyl-coenzyme A (acyl-CoA) to an acceptor. ATs play important roles in the maintenance of homeostasis in the human body and have been linked to various diseases; therefore, several ATs have been proposed as potential targets for the treatment or prevention of such diseases. The AT family includes acyl-CoA:cholesterol AT (ACAT), diacylglycerol AT, and monoacylglycerol AT for the metabolism of lipids. Furthermore, recent molecular biological studies revealed the existence of their isozymes with distinct functions in the body. ACAT plays a critical role in the formation of cholesteryl esters from cholesterol and fatty acids, and is a potential target for treating hypercholesterolemia. During an experiment designed to discover biologically active compounds from herbal medicines, we isolated two known guaianolide sesquiterpene lactones from Chrysanthemum boreale Makino (Compositae). The lactones were characterized from their spectroscopic data (NMR, IR, MASS). These compounds were subjected to ACAT inhibition assay. Here, we report the isolation and structural elucidation of the compounds 8-o-acetyl-2-methoxy-10-hydroxy-3,11(13)-guaiadiene-12,6-olide and 8-acetyl-3,10-hydroxy-4(15),11(13)-guaiadiene-12,6-olide. In the ACAT inhibition assay, compound 1 showed strong inhibitory activity, with an $IC_{50}$ value $45{\mu}g/mL$, whereas compound 2 did not exhibit significant inhibitory activity with an over $100{\mu}g/mL$.

Effect of Hydrosol Extracted from Chrysanthemum boreale Makino Flower on Proliferation and Migration in Human Skin Keratinocyte (산국화(Chrysanthemum boreale Makino) 유래 Hydrosol의 피부 각질형성세포 증식 및 이주 유도 활성에 미치는 효과)

  • Kim, Do Yoon;Hwang, Dae Il;Yoon, Mi-so;Choi, In Ho;Lee, Hwan Myung
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.42 no.1
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    • pp.95-101
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    • 2016
  • In the present study, we extracted the hydrosol from flower of Chrysanthemum boreale Makino (CBMF hydrosol) by steam distillation and tested the effect of the CBMF hydrosol on skin regeneration using normal human keratinocytes (HaCaTs). CBMF hydrosol induced proliferation as well as migration in HaCaTs in a dose-dependent manner. Treatment with $1{\mu}g/mL$ CBMF hydrosol increased proliferation to $143.71{\pm}3.37%$ and migration to $139.98{\pm}5.72%$ compared with a control group. CBMF hydrosol also significantly enhanced the phosphorylations of extracellular signal-regulated kinase (Erk) 1/2 and serine/threonine-specific protein kinase (Akt) in HaCaTs. Moreover, CBMF hydrosol dose-dependently induced sprout outgrowth in HaCaTs. These results demonstrate that CBMF hydrosol has skin regeneration and wound healing activity in HaCaTs. Therefore, CBMF hydrosol could be used as a potential cosmetic material.

Effects of Sesquiterpene Lactones Isolated from Chrysanthemum boreale M. against Sarcoma180 Implanted in ICR Mice (산국으로부터 분리한 Sesquiterpene Lactones의 흰쥐 복수암에 대한 효과)

  • 남상해;최상도;최진상;장대식;최상욱;양민석
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.26 no.1
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    • pp.144-147
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    • 1997
  • For the investigation of antitumor agents, two kinds of sesquiterpene lactones were isolated and purified from Chrysanthemum boreale M. and designated as Compound I and ]U . And then in vivo antitumor test of the sesquiterpene lactones was carried out against ICR mice. In vivo test against Sarcoma180 implanted ICR mice, life prolongation effects of Compound I and II were showed as 143% and 134% at the dose of 10mg/kg, respectively. Besides, at the tested group mixed Compound I and II each 1mg/kg was showed rather high life prolongation effect as 158%.

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Biological Activities of Sesquiterpene Lactones isolated from Several Compositae Plants. Part 2 -FPTase Inhibitory Activity- (수종의 국화과 식물에서 분리한 Sesquiterpene Lactone들의 생리활성(제2보) -FPTase 저해활성-)

  • Jang, Dae-Sik;Kwon, Byong-Mog;Yang, Min-Suk
    • Korean Journal of Pharmacognosy
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    • v.30 no.1
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    • pp.70-73
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    • 1999
  • Nine sesquiterpene lactones, which were isolated from Hemisteptia lyrata Bunge. Chrysanthemum zawadskii Herbich var. latilobum Kitamura and Chrysanthemum boreale Makino were evaluated for the farnesyl-protein transferase (FPTase) inhibitor, conducted by the scintillation proximity assay (SPA). The angeloylcumambrin B and tigloylcumambrin B inhibited a recombinant rat FPTase with $IC_{50}$ value of $78\;{\mu}g/ml$ $(225\;{\mu}M)$ and $90\;{\mu}g/ml$ $(260 \;{\mu}M)$, respectively.

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Antioxidant Activity and Protective Effects of Extracts from Chrysanthemum boreale on t-BHP Induced Oxidative Stress in Chang Cells (산국대 추출물의 항산화 활성 및 간세포 보호 효과)

  • Kim, Yon-Suk;Hwang, Jin-Woo;Park, Pyo-Jam;Jeong, Jae-Hyun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.1
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    • pp.60-66
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    • 2014
  • The aim of this study was to evaluate the antioxidant activity and protective effect of extracts from the stems and leaves of Chrysanthemum boreale (CBSL) on t-BHP induced oxidative stress in human liver cells (Chang cells). Antioxidant activities in the extracts were determined for various radical scavenging activities including ferric reducing antioxidant power, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity, and oxygen radical absorbance capacity (ORAC). CBSL showed a very good scavenging effect of DPPH radical ($IC_{50}$ $0.009{\pm}0.002$ mg/mL), alkyl radical ($IC_{50}$ $0.004{\pm}0.001$ mg/mL), and hydroxyl radical ($IC_{50}$ $6.742{\pm}0.152$ mg/mL). CBSL also showed a strong antioxidant effect in the ORAC assay. In the MTT assay on human liver cells (Chang cells), the extracts showed protective effects by increasing cell viability, decreasing ROS, and restoring mitochondria membrane potential upon t-BHP induced oxidative stress. Our findings suggest that CBSL extracts are a potential therapeutic with protective antioxidant effects upon oxidative stress.