• Title, Summary, Keyword: Chrysanthemum boreale M.

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Isolation of Cytotoxic Substances from Chysanthemum Boreale M. (산국으로부터 항암활성 성분의 분리)

  • Yang, Min-Suk;Nam, Sang-Hae
    • Applied Biological Chemistry
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    • v.38 no.3
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    • pp.273-277
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    • 1995
  • Sixty kinds of medicinal plants were examined upon the cytotoxicity against L1210 mouse leukemia cells. Isolation and purification of effective antitumor substances from Chysanthemum boreale had been performed which appeared strong cytotoxicity, In cytotoxicity test of the each solvent fractions, $ED_{50}$ values of chloroform fraction against L1210, K562 and A549 cells were shown as 3.98, 4.28, 3.84 (${\mu}g/ml$), respectively. Compound I and II were purified from the chloroform fraction. Between the purified compounds, $ED_{50}$ values of Compound I against L1210, K562 and A549 cells were shown as 0.55, 0.0003, 0.001 (${\mu}g/ml$), respectivety. Whereas Compound II was shu as 4.79 against K562.

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A Study on the Activities of Five Natural Plant Essential Oils on Atopic Dermatitis (자생식물 Essential Oil 5 종의 항 아토피피부염 활성 연구)

  • Jeong, Jeong-Hwa;Nguyen, Thao Kim Nu;Choi, Min-Jin;Nguyen, Ly Thi Huong;Shin, Heung-Mook;Lee, Byung-Wook;Yang, In-Jun
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.47 no.1
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    • pp.23-30
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    • 2021
  • This study is an experiment to evaluate the anti-atopy efficacy of five kinds of natural plant essential oils; Artemisia annua L. (AA), Citrus junos Sieb. ex TANAKA (CJ), Chrysanthemum boreale Makino (CB), Pinus koraiensis (PK), and Pinus densiflora for. erecta (PD). Through Agar diffusion test, five species of native plant essential oils were treated in a total of four strains, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans. In order to invest the anti-inflammatory effect, five kinds of natural plant essential oils were treated in HaCaT cells-induced by TNF-α and IFN-γ (TI). AA, CJ, CB, PK and PD showed antibacterial effects on Candida albicans at a concentration of 10 mg/mL. We also found that the thymus and activation-regulated chemokine (TARC) expression was suppressed in 0.1 ㎍/mL of PK, 1 ㎍/mL of AA, CB, and PK. macrophage-derived chemokine (MDC) expression was suppressed in 1 ㎍/mL of AA and PK. IL-6 expression was suppressed in 0.1, 1 ㎍/mL of AA, PK in HaCaT cells. Hence it suggests that AA, CB, and PK have the anti-inflammatory effects, and it could contribute to atopic dermatitis relief by reducing the infiltration of immune cells to inflamed area.

Quality Characteristics and Antioxidant Activity of Chrysanthemum indicum L., C. boreale M., and C. zawadskii K. Flowers Candies (감국, 산국 및 구절초꽃 캔디의 항산화활성과 품질특성)

  • Lee, Sang-Hoon;Hwang, In-Guk;Lee, Ha-Kyoo;Shin, So-Lim;Chang, Young-Deug;Lee, Chul-Hee;Jeong, Heon-Sang
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.10
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    • pp.1406-1413
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    • 2009
  • In order to investigate the quality characteristics of candies prepared with Compositae species flower extracts, the samples of Chrysanthemum indicum Linne (CI), C. boreale Makino (CB), and C. zawadskii var. latilobum (Maxim.) Kitam (CZ) were extracted with hot water and concentrated, and then added with various concentrations of 5$\sim$20% for candy preparation. L-value of candy color was decreased, but a- and b-value were increased with increasing amount of added extracts. pH was decreased, but total acidity was increased with increasing amount of added extracts. Moisture content of CI, CB, and CZ candies were 2.61$\sim$4.11, 2.46$\sim$4.57, and 2.27$\sim$4.43%, respectively. Hardness of candies were decreased with increasing amount of added extracts. CZ candy of 20% added extract showed the highest total polyphenol, flavonoid content, electron donating ability and anol, flavonaid content at 2.71 mg/g, 0.86 mg/g, 76.24%, and 3.17 mg AA eq/g, respectively. In sensory evaluation, the CI and CZ candies of 10% added extract obtained higher sensory scores.

Biological Activities of Sesquiterpene Lactones isolated from Several Compositae Plants Part 3 -Inhibitory Activity on Nitric Oxide Release and ACAT- (수종의 국화과 식물에서 분리한 Sesquiterpene Lactone들의 생리활성(제3보) -Nitric oxide 방출 및 ACAT 저해활성-)

  • Jang, Dae-Sik;Park, Ki-Hun;Ko, Hack-Lyong;Lee, Hyun-Sun;Kwon, Byoung-Mog;Yang, Min-Suk
    • Korean Journal of Pharmacognosy
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    • v.30 no.1
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    • pp.74-78
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    • 1999
  • Nine sesquiterpene lactones, which were isolated from Hemisteptia lyrata Bunge, Chrysanthemum zawadskii Herbich var. latilobum Kitamura and Chrysanthemum boreale Makino were evaluated for the inhibition upon the nitric oxide release from the Raw cell and for the ACAT (acyl Co A: cholesterol acyltransferase) inhibitory assay. In the nitric oxide release inhibitory experiment, hemistepsin B $(IC_{50}\;=\;0.05\;{\mu}g/ml,\;0.15\;{\mu}M),$ cumambrin B, tulipinolide and costunolide exhibited strong inhibition with $IC_{50}$ values $0.05\;{\mu}g/ml\;(0.15\;{\mu}M),\;0.1\;{\mu}g/ml\;(0.38\;{\mu}M),\;0.2\;{\mu}g/ml\;(0.69\;{\mu}M)$ and $0.2\;{\mu}g/ml(0.86\;{\mu}M),$ respectively. In the ACAT inhibitory assay, angeloylcumambrin B, tigloylcumambrin B, tulipinolide and costunolide exhibited strong inhibition with $IC_{50}$ values $33\;{\mu}g/ml\;(95.4\;{\mu}M),\;22\;{\mu}g/ml\;(63.6\;{\mu}M),\;38\;{\mu}g/ml\;(151\;{\mu}M)$ and $17\;{\mu}g/ml(73.3\;{\mu}M),$ respectively, whereas other compounds did not exhibit significant activity against ACAT above $100\; {\mu}g/ml.$.

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Effect of cumambrin A Treatment on Blood Pressure in Spontaneously Hypertensive Rats (고혈압 흰쥐에서 cumambrin A의 혈압 강하 효과)

  • Lee Han-ki;Hong Yong-geun
    • The Journal of Korean Physical Therapy
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    • v.14 no.3
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    • pp.297-307
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    • 2002
  • We have isolated a sesquiterpene lactone, cumambrin A from the dried flowers of Chrysanthemum boreale Makino and reported its chemical structure. The aim of the present study was to investigate whether the exogenous administration of cumambrin A has a pharmacological effect on normalization of blood pressure in the spontaneously hypertensive rats (SHR). In vitro studies: Relaxative response induced by cumambrin A was increased with dose-dependent manner and showed maximizing response at a concentration of 5?0-4 M. Further, this relaxative response was significantly increased at a condition of endothelium present than that of endothelium denuded. in vivo studies: The normalizing effect of cumambrin A on blood pressure was also increased with time-dependent manner and then gradually recovered to normal condition at approximately 4 hrs after cumambrin A treatment.

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Effect of Cumambrin A Treatment on Blood Pressure in Spontaneously Hypertensive Rats (고혈압 흰쥐에서 Cumambrin A의 혈압 강하 효과)

  • Hong, Yong-Geun;Yang, Min-Suk;Pak, Yun-Bae
    • Korean Journal of Pharmacognosy
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    • v.30 no.2
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    • pp.226-230
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    • 1999
  • We have isolated a sesquiterpene lactone, cumambrin A from the dried flowers of Chrysanthemum boreale Makino and reported its chemical structure. The aim of the present study was to investigate whether the exogenous administration of cumambrin A has a pharmacological effect on normalization of blood pressure in the spontaneously hypertensive rats (SHR). In vitro studies: Relaxative response induced by cumainbrin A was increased with dose-dependent manner and showed maximizing response at a concentration of $5{\times}10^{-4}M$. Further, this relaxative response was significantly increased at a condition of endothelium present than that of endothelium denuded. In vivo studies: The normalizing effect of cumambrin A on blood pressure was also increased with time-dependent manner and then gradually recovered to normal condition at approximately 4 hrs after cumambrin A treatment.

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Effect of Cumambrin A on the Relaxation of Rat Aorta (흰쥐에서 Cumambrin A의 대동맥 이완작용)

  • Hong, Yong-Geun;Yang, Min-Suk;Pak, Yun-Bae
    • Korean Journal of Pharmacognosy
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    • v.36 no.1
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    • pp.17-20
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    • 2005
  • We previously reported that the exogenous administration of cumambrin A, a sesquiterpene lactone from the dried flowers of Chrysanthemum boreale Makino has a pharmacological effect on normalization of blood pressure in the spontaneously hypertensive rats (SHR). In the present study, we further investigated the effect of cumambrin A on the relaxation of phenylephrine-induced precontracted rat aortic artery rings. The potency of cumambrin A was than compared to verapamil, a well known $Ca^{2+}-channel$ blocker. The results demonstrate that the isolated rat aortic arteries are relaxed to basal tension at a concentration of $5{\times}10^{-5}\;M$ cumambrin A treatment. The results also show that the phenylephrine-induced contraction is inhibited by a pretreatment of cumambrin A. Co-treatment of cumambrin A and verapamil showed a strong synergetic effect on the relaxation of rat aortic artery rings. Thus, these data demonstrate that cumambrin A is a potent relaxant of rat aortic smooth muscle and suggest that cumambrin A modulates intracellular or extracellular $Ca^{2+}$ mobilization.

Induction of Quinone Reductase, an Anticarcinogenic Marker Enzyme, by Medicinal Herb Extracts

  • Kwon, Chong-Suk;Kim, Ji-Hyeon;Son, Kun-Ho;Kim, Young-Kyoon;Lee, Jeong-Soon;Lim, Jin-Kyu;Kim, Jong-Sang
    • Preventive Nutrition and Food Science
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    • v.7 no.4
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    • pp.358-366
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    • 2002
  • To search for novel cancer preventive agents, we assessed the quinone reductase (QR)-inducing activities of medicinal herb extracts in cultured murine hepatoma cells (hepalclc7 cells). Among 216 herb extracts tested in this study, 8 kinds of herbal extracts were found to induce QR activity in hepalclc7 cells by more than 2-fold when used at the concentration of 25 $\mu\textrm{g}$/$m\ell$. The methanol extracts of Aster koraiensis NK and Pulsatilla koreana Nakai induced QR by 252 and 223 % , respectively, at the concentration of 25 $\mu\textrm{g}$/$m\ell$. Most of the herbal extracts with QR inducing-activity increased the enzyme activity in a typical dose-dependent manner. The QR activity in BP$^{r}$ cl cells was induced move than 50 % by the extracts of Pulsatilla koreana Nakai, Inula helenium, Physalis alkekengi var, francheti (Masters) Makino, Chrysanthemum zawadskii var. latilobum Kitamuva, Auemisia keiskeana Miquel, Chfsanthemum boreale Makino. In conclusion, hlsatilla koreana Nakai, Aster koraiensis N.K, and Chfsanthemum zawadskii var. iatilobum Kitamura, which showed relatively high QR induction, merit further animal study to evaluate their potential as cancer preventive agents.