• Microbiology and Biotechnology Letters
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• v.35 no.3
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• pp.220-225
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• 2007

In order to develop a potent antidandruffy agent from medicinal plants, various extracts of medicinal plants and herbs were prepared and investigated its antifungal activity against Malassezia furfur. Among 85 kinds of the secondary screened medicinal plants and herbs, water extract of Chrysanthemum zawadskii had the highest antifungal activity against dandruffy-producing Malassezia furfur. The antifungal compound was also maximally extracted when the powder of Chrysanthemum zawadskii was treated with water at $50^{\circ}C$ for 6 hours. The antifungal compound from Chrysanthemum zawadskii was partially purified by silica gel column chromatography and the partial purified compound was soluble at $70^{\circ}C$ for 20 min and pH $3.0{\sim}9.0$.

• Culinary science and hospitality research
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• v.23 no.2
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• pp.117-125
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• 2017

This study investigated the physicochemical and sensory characteristics of yanggaeng prepared with various amounts of Chrysanthemum zawadskii powder (as ratios of 0.5%, 1%, 1.5%, 2% to the total materials). The result displayed that the $^{\circ}Brix$ value, pH of yanggaeng decreased significantly with the addition of Chrysanthemum zawadskii powder. Increasing the amount of Chrysanthemum zawadskii powder in the yanggeng tended to increased acidity. In chromaticity determination, the values of lightness (L) showed a decrease. However, the value of redness (a) increased by increasing levels of Chrysanthemum zawadskii powder. Texture measurement scores in terms of hardness, springiness, and adhesiveness for yanggaeng showed that sample groups were lower than those of the control group. Cohesiveness and chewiness were higher in the additive group than in the control group. DPPH radical scavenging activity and nitrites cavenging activity were significantly increased Chrysanthemum zawadskii (p<0.001). The DPPH content was highest in the 62.40% Chrysanthemum zawadskii yanggaeng. As Chrysanthemum zawadskii powder increased, antioxidative activity also increased. Sensory evaluation scores in terms of color, odor, taste, texture, and overall preference of groups with 0.5% of Chrysanthemum zawadskii powder did not show any significant differences when compared to the control group. Based on the above results, this study suggests that the addition of 0.5% Chrysanthemum zawadskii powder may be the best substitution ratio for yanggaeng.

• Preventive Nutrition and Food Science
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• v.17 no.3
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• pp.234-238
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• 2012

The volatile aroma constituents of Chrysanthemum zawadskii var. latilobum K. were separated by hydro distillation extraction (HDE) method using a Clevenger-type apparatus, and analyzed by gas chromatography-mass spectrometry (GC/MS). The yield of C. zawadskii var. latilobum K. flower essential oil (FEO) was 0.12% (w/w) and the color was light green. Fifty-five volatile chemical components, which make up 88.38% of the total aroma composition, were tentatively characterized. C. zawadskii var. latilobum K. FEOs contained 27 hydrocarbons, 12 alcohols, 7 ketones, 4 esters, 1 aldehyde, 1 amine, and 3 miscellaneous components. The major functional groups were terpene alcohol and ketone. Borneol (12.96), (${\pm}$)-7-epi-amiteol (12.60), and camphor (10.54%) were the predominant volatiles. These compounds can be used in food and pharmaceutical industries due to their active bio-functional properties.

• Natural Product Sciences
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• v.14 no.2
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• pp.138-142
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• 2008

The composition of essential oils from Chrysanthemum zawadskii var. latilobum and C. indicum were analyzed and compared. The results of gas chromatography-mass spectrometry revealed there were distinctly different compositional patterns between C. zawadskii var. latilobum and C. indicum essential oils. The combinatorial effect of the oil of C. zawadskii var. latilobum and C. indicum, with various antibiotics was assessed against antibiotic-susceptible and -resistant strains of Staphylococcus aureus and Streptococcus pneumoniae. The essential oil fraction significantly inhibited most of the tested antibiotic-susceptible and -resistant strains of S. pneumoniae, with minimum inhibiting concentrations (MICs) ranging from 0.5 to 4.0 mg/ml. The fractional inhibiting concentration indices (FICIs) of the oils when combined with antibiotics against S. aureus and S. pneumoniae ranged from 0.26 to 0.75, and showed synergistic or additive effects.

• Journal of the Korean Society of Food Science and Nutrition
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• v.38 no.7
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• pp.824-831
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• 2009

In order to investigate the quality characteristics of powdered teas using Compositae species flower, the samples of Chrysanthemum indicum L. (CI), Chrysanthemum boreale Makino (CB), and Chrysanthemum zawadskii var. latilobum (Maxim.) Kitam (CZ) were extracted with hot water and concentrated, and then mixed with different forming agents of dextrin (D) and maltodextrin (MD). The mixing ratio of D and MD was ranged from 9:1 (DMD91) to 8:2 (DMD82) or D and MD only. The solubility of the powdered tea was higher in added dextrin. The highest total polyphenol and flavonoid content of the powdered tea were 6.75 and 3.24 mg/g at CBDMD91, respectively. Total antioxidant activities of C. indicum, C. boreale, and C. zawadskii powdered tea ranged at $2.51{\sim}2.63$, $4.37{\sim}4.50$, and $3.44{\sim}3.55\;mg$ AA eq/g, respectively. In sensory evaluation, the C. indicum and C. zawadskii powdered teas obtained higher sensory score of all evaluation items than C. boreale powdered tea. The optimal mixing ratio at 36% of D and 4% of MD in forming agent was selected to enhance the sensory characteristics and antioxidant activities of powdered tea.

• The Korean Journal of Food And Nutrition
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• v.31 no.3
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• pp.378-387
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• 2018

This study investigated the volatile flavor composition of essential oils from Chrysanthemum zawadskii var. latilobum Kitamura and Aster yomena Makino. The essential oils obtained by the hydrodistillation extraction method from the aerial parts of the plants were analyzed by gas chromatography (GC) and GC-mass spectrometry (GC-MS). One hundred (95.04%) volatile flavor compounds were identified in the essential oil from the C. zawadskii var. latilobum Kitamura. The major compounds were valencene (10.82%), ${\delta}$-cadinol (9.77%), hexadecanoic acid (8.70%), 2-methyl-4-(2,6,6-trimethylcyclohex-1-enyl) but-2-en-1-ol (3.67%), and 2-(2,4-hexadiynylidene)-1,6-dioxaspiro[4,4]non-3-ene (3.57%). Ninety-eight (93.83%) volatile flavor compounds were identified in the essential oil from the Aster yomena Makino. The major compounds were and 3-eicosyne (13.61%), 9,10,12-octadecatrienoic acid (7.8%), ${\alpha}$-caryophyllene alcohol (6.83%), 9-octadecynoic acid (6.03%), and ${\alpha}$-caryophyllene (5.74%). Although the two plants are apparently very similar, the chemical composition of the essential oils was significantly different in quality and quantity. In the case of C. zawadskii var. latilobum Kitamura, the sesquiterpene, valencene was found to be 10.82%, but it was not identified in A. yomena Makino. ${\delta}$-Cadinol appeared higher in C. zawadskii var. latilobum Kitamura than in A. yomena Makino. A clear characteristic of A. yomena Makino essential oil is that it has a high content of caryophyllene derivatives. The ${\alpha}$-caryophyllene alcohol contained in A. yomena Makino was relatively high at 6.83%, although the compound was not identified in C. zawadskii var. latilobum Kitamura. Also ${\alpha}$-caryophyllene was shown to be higher in A. yomena Makino than in C. zawadskii var. latilobum Kitamura.

• The Korean Journal of Food And Nutrition
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• v.29 no.3
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• pp.431-437
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• 2016

Macrophages play a pivotal role in the innate and adaptive immune systems. This study investigated the immuno-modulatory activities of polysaccharides separated from Chrysanthemum zawadskii var. latilobum (CZPS) in macrophages. Polysaccharides from Chrysanthemum zawadskii var. latilobum were extracted by the ethanol precipitation method. RAW 264.7 mouse macrophage cell line was treated with CZPS (4 to $128{\mu}g/mL$), and there was no cytotoxicity at a dose below $32{\mu}g/mL$. The levels of nitric oxide (NO) and pro-inflammatory cytokines (tumor necrosis factor (TNF)-${\alpha}$ and interleukin (IL)-6, IL-$1{\beta}$) production in the CZPS treated group ($32{\mu}g/mL$) were $6.5{\pm}0.12{\mu}m$ (NO), $1252.8{\pm}79.85$ (TNF-${\alpha}$), $305.4{\pm}29.41$ (IL-6), and $683.3{\pm}59.71$ (IL-$1{\beta}$), respectively, and they were significantly increased when compared to the control group; $2.2{\pm}0.03{\mu}m$ (NO), $452.3{\pm}38.34$ (TNF-${\alpha}$), $31.7{\pm}5.75$ (IL-6), and $184.1{\pm}11.52$ (IL-$1{\beta}$). Additionally, protein expression of inducible nitric oxide synthase (iNOS) and phosphorylation of MAPKs and NF-${\kappa}B$ expression were significantly increased upon CZPS treatment. Therefore, these results indicated that polysaccharides separated from Chrysanthemum zawadskii var. latilobum (CZPS) may have a potential immunomodulatory activity in macrophages through MAPKs and NF-${\kappa}B$ signaling, and this information is useful for the development of immune enhancing adjuvant materials using a natural ingredient.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.9 no.6
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• pp.86-94
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• 2006

This study was to analyze vegetation and soil characteristic, and ordination of Bupleurum euphorbioides community located in Mt. Seorak area, Mt. Sobaek area, and Mt. South Deogyu area. Bupleurum euphorbioides community was classified into Carex lanceolata subcommunity and Chrysanthemum zawadskii var. alpinum subcommunity, and Chrysanthemum zawadskii var. alpinum subcommunity was classified into Calamagrostis arundinacea and Carex breviculmis group. In the study sites, soil organic matter, nitrogen, available phosphorous, changeable potassium, changeable calcium, changeable magnesium concentration, cation exchange capacity, and soil pH were ranged from 9.0~15.2%, 0.32~0.47%, 3.0~8.6ppm, 0.3~0.9me/100g, 0.5~1.5me/100g, 0.2~0.6me/100g, 11.4~16.1me/100g, and 4.3~4.6, respectively. And, soil texture was sandy loam or loam. The Carex breviculmis group of Chrysanthemum zawadskii var. alpinum subcommunity was found in area of high elevation and had high exchangeable K, Ca, and Mg concentration. The Calamagrostis arundinacea group of Chrysanthemum zawadskii var.alpinum subcommunity was found in area of low elevation and had lower exchangeable K, Ca, and Mg concentration than in the other subcommunity. The Carex lanceolata subcommunity was found in area of medium elevation and had medium exchangeable K, Ca, and Mg concentration. The Carex lanceolata subcommunity was located in a path of top the mountain peak, and has been destroyed. We need any action to protect the subcommunity.

• Preventive Nutrition and Food Science
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• v.10 no.4
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• pp.340-343
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• 2005

Induction of NAD(P)H:(quinone-acceptor) oxidoreductase (QR) which promotes obligatory two electron reduction of quinones and prevents their participation in oxidative cycling and thereby the depletion of intracellular glutathione, has been used as a marker for chemopreventive agents. Induction of phase II enzyme is considered to be an important mechanism of cancer prevention. In our previous study, we assessed the quinone reductase QR-inducing activities of 216 kinds of medicinal herb extracts in cultured murine hepatoma cells, BPRc1 and hepalc1c7 cells. Among the 216 herbal extracts tested in that study, extracts from Chrysanthemum zawadskii showed significant induction of QR. In this study, we examined QR-inducing activity of solvent fractions of the herbal extract. The dichloromethane fraction of the herb showed the highest QR induction among the samples fractionated with four kinds of solvents with different polarity. The fraction also significantly induced the activity of glutathione S-transferase (GST), one of the major detoxifying enzymes, at $4{\mu}g/mL\;and\;2{\mu}g/mL$ in hepalc1c7 and BPRc1 cells, respectively. In conclusion, dichloromethane-soluble fraction of Chrysanthemum zawadskii which showed relatively strong induction of detoxifying enzymes merits further study to identify active components and evaluate their potential as cancer preventive agents.

• Applied Biological Chemistry
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• v.42 no.2
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• pp.176-179
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• 1999

In the bioactivity test of nine sesquiterpene lactons isolated from Hemisteptia lyrata, Chrysanthemum zawadskii and Chrysanthemum boreale, we investigated their antimicrobial activities against ten bacteria and six phytopathogenic fungi. Hemistepsin A and hemistepsin B isolated from H. lyrata and tulipinolide isolated from C. boreale exhibited strong antibacterial activity. In the antifungal test, hemistepsin B, angeloylcumambrin B, tigloylcumambrin B and costunolide showed broad activity. Both antibacterial and antifungal experiments exhibited quite clear specificity against microbial strains.