• Title, Summary, Keyword: Coptidis rhizoma extract

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The Experimental Study on Anti-bacterial Potency of Coptidis rhizoma extract on Staphylococcus aureus & Staphylococcus epidermidis (황련 전탕액이 Staphylococcus aureus와 Staphylococcus epidermidis에 미치는 항균효과에 대한 실험적 연구)

  • Seo, Hyung-Sik
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.19 no.2
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    • pp.71-76
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    • 2006
  • Objective : This experimental study was performed to investigate the effect of Coptidis rhizoma extract on Staphylococcus aureus and Staphylococcus epidermidis that induce keratitis. Methods : MIC was measured by flowing to $50{\mu}l$ according to density Coptidis rhizoma extract(100%, 10%, 1%, 0.1%). Anti-bacterial potency was measured by the size of inhibition zone with change of volume. Results : 1. MIC on Staphylococcus aureus in Coptidis rhizoma extract was 100%, $50{\mu}l$ and anti-bacterial potency increase compare with quantity. 2. MIC on Staphylococcus epidermidis in Coptidis rhizoma extract was 100% & 10%, $50{\mu}l$ and anti-bacterial potency increase compare with quantity. Conclusions : The Present author think that Coptidis rhizoma extract must be made a new eye drops.

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황련의 증량에 따른 전탕액이 Pseudomonas aeruginosa에 미치는 항균효과에 대한 실험적 연구

  • Seo, Hyeong-Sik
    • Journal of Pharmacopuncture
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    • v.10 no.1
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    • pp.17-21
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    • 2007
  • Objective : This experimental study was performed to investigate the effect of Coptidis rhizoma extract compared with quantity on Pseudomonas aeruginosa that induce keratitis. Methods : MIC was measured by dropping to 50 ${\mu}l$ according to density Coptidis rhizoma extract(100%, 10%, 1%, 0.1%) compared with quantity(40g, 80g, 120g, 160g). Anti-bacterial potency was measured by the size of inhibition zone with change of volume. Results : MIC on Pseudomonas aeruginosa in Coptidis rhizoma extract compared with quantity(40g, 80g, 120g, 160g) was not showed all samples(100%, 10%, 1%, 0.1%). Conclusions : Coptidis rhizoma extract compared with quantity was not showed anti-bacterial potency on Pseudomonas aeruginosa. The results differ that Coptidis rhizoma extract was showed anti-bacterial potency in 1999 year. Anti-bacterial Potency of Coptidis rhizoma extract compared with quantity on Pseudomonas aeruginosa must be studied.

Inhibitory effects of Coptidis Rhizoma on the LPS-induced production of nitric oxide and $TNF-{\alpha}$ in mouse macrophage cells (황련의 쥐 대식세포로부터 LPS에 의해 유도되는 nitric oxide 및 $TNF-{\alpha}$의 생성억제효과)

  • Jung, Hyo-Won;Park, Yong-Ki
    • The Korea Journal of Herbology
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    • v.21 no.2
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    • pp.165-173
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    • 2006
  • Objectives : Coptidis Rhizoma has been known traditional medicine with antimicrobial activities. We investigated inhibitory effects of Coptidis Rhizoma extract on lipopolysaccharide(LPS)-induced nitric oxide production from mouse macrophages. Methods : After Coptidis Rhizoma extract was pretreated in BV2, mouse brain macrophages and RAW264.7 mouse macrophages, cells were activated with LPS. To investigate cytotoxicity Coptidis Rhizoma extract, cell viability was measured by MTT assay. The production of nitric oxide(NO) and inducible nitric oxide synthase(iNOS) was determined in each culture supernatant and mRNA by Griess reaction and RT-PCR. The production of $TNF-{\alpha}$ from cells was measured by ELISA. Results : Coptidis Rhizoma extract significantly inhibited LPS-induced NO production in BV2 and RAW264.7 cells. Coptidis Rhizoma extract also greatly suppressed mRNA expression of iNOS in BV2 and RAW264.7 cells activated by LPS. Conclusion : These data suggests that Coptidis Rhizoma extract may have an anti-inflammatory effect through the inhibition of NO production.

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The Experimental Study on Anti-bacterial Potency of Coptidis rhizoma extract compare with quantity on Staphylococcus aureus (황련의 증량에 따른 전탕액이 Staphylococcus aureus에 미치는 항균효과에 대한 실험적 연구)

  • Seo, Hyeong-Sik
    • Journal of Pharmacopuncture
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    • v.10 no.3
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    • pp.47-52
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    • 2007
  • Objective This experimental study was performed to investigate the effect of Coptidis rhizoma extract compared with quantity on Staphylococcus aureus that induce keratitis. Methods Minimal inhibitory concentration(MIC) was measured by dropping to $50{\mu}l$ according to density Coptidis rhizoma extract(100%, 10%, 1%, 0.1%) compared with quantity(40g, 80g, 160g). Anti-bacterial potency was measured by the size of inhibition zone with change of volume. Results 1. MIC on Staphylococcus aureus in Coptidis rhizoma extract was showed anti-bacterial potency compared with quantity and density in 100% and 10% of all samples(40g, 80g, 160g). 2. MIC on Staphylococcus aureus in Coptidis rhizoma extract(40g, 80g, 160g) was showed anti-bacterial potency compared with quantity all samples($20{\mu}l,\;30{\mu}l,\;40{\mu}l,\;50{\mu}l$) in 100% density. 3. MIC on Staphylococcus aureus in Coptidis rhizoma extract(40g, 80g, 160g) was showed anti-bacterial potency compared with quantity all samples ($20{\mu}l,\;30{\mu}l,\;40{\mu}l,\;50{\mu}l$) in 100% density. Anti-bacterial potency of 80g Coptidis rhizoma extract decreased compared with 40g. Anti-bacterial potency of 160g Coptidis rhizoma extract decreased compared with 40g in $20{\mu}l,\;30{\mu}l$. Conclusions Coptidis rhizoma extract was showed anti-bacterial potency compare with quantity and density. In herbal drug, antibacterial potency compare with quantity and density must be studied.

황련의 증량에 따른 전탕액이 Staphylococcus epidermidis에 미치는 항균효과에 대한 실험적 연구

  • Seo, Hyeong-Sik
    • Journal of Pharmacopuncture
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    • v.10 no.1
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    • pp.79-84
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    • 2007
  • Objective : This experimental study was performed to investigate the effect of Coptidis rhizoma extract compared with quantity on Staphylococcus epidermidis that induce keratitis. Methods : MIC was measured by dropping to 50${\mu}l$ according to density Coptidis rhizoma extract(100%, 10%, 1%, 0.1%) compared with quantity(40g, 80g, 160g). Anti-bacterial potency was measured by the size of inhibition zone with change of volume. Results : 1. MIC on Staphylococcus epidermidis in Coptidis rhizoma extract was showed anti-bacterial potency compared with quantity and density in 100% and 10% of all samples(40g, 80g, 160g). 2. MIC on Staphylococcus epidermidis in Coptidis rhizoma extract(40g, 80g, 160g) was showed anti-bacterial potency compared with quantity all samples($20{\mu}l$, $30{\mu}l$, $40{\mu}l$, $50{\mu}l$) in 100% density, but anti-bacterial potency of 80g, $50{\mu}l$ Coptidis rhizoma extract decreased compared with 40g, $50{\mu}l$. 3. MIC on Staphylococcus epidermidis in 40g Coptidis rhizoma extract was showed in $50{\mu}l$, $40{\mu}l$ of 10% density, 80g Coptidis rhizoma extract was showed in $50{\mu}l$ of 10% density, 160g Coptidis rhizoma extract was showed in $50{\mu}l$, $40{\mu}l$, $30{\mu}l$ of 10% density. Conclusions : Coptidis rhizoma extract was showed anti-bacterial potency compare with quantity and density. In herbal drug, anti-bacterial potency compare with quantity and density must be studied.

Effects of subfractions of Coptidis Rhizoma extract on the nitric oxide production in LPS-stimulated BV2 microglial cells (황련 추출물의 분획화 및 BV2 microglial cells에서 LPS에 의해 유도되는 nitric oxide 생성억제효과 검정)

  • Jung, Hyo-Won;Park, Yong-Ki
    • The Korea Journal of Herbology
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    • v.22 no.2
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    • pp.73-78
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    • 2007
  • Objectives : Uncontrolled activation of microglia may directly toxic to neurons by releasing various substances such as inflammatory cytokines, nitric oxide(NO), prostaglandin E2 and superoxide. In this study, the effects of the several subfractions isolated from Coptidis Rhizoma extract were investigated on NO production in LPS-stimulated BV2 microglial cells, Methods : Coptidis Rhizoma extract prepared with 80% methanol, and then fractionated with ethylacetate, chloroform, n-butanol and water. BV2 cells were pretreated four subfractions of Coptidis Rhizoma with various concentrations, and then stimulated with LPS. Cytotoxicity of each fraction was measured by MTT assay. NO production was determined in culture surpernatants by Griess reagent. Results : Ethylacetate, chloroform and butanol fractions of Coptidis Rhizoma extract significantly decreased LPS-induced NO production in BV2 cells as a dose-dependent manner without cytotoxicity. Ethylacetate fraction of Coptidis Rhizoma extract was most effective on inhibition of NO production in LPS-stimulated BV2 cells compared with other fractions. Conclusion : This data indicates that Ethylacetate fraction of Coptidis Rhizoma extract shows strong antiinflammatory effects through inhibition of LPS-induced microglial activation.

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The Experimental Study on the continuous Anti-bacterial Potency of Coptidis rhizoma extract on Cultivation of Staphylococcus species(S. aureus, S. epidermidis) (황련(黃連) 전탕액(煎湯液)이 Staphylococcus species(S.aureus, S.epidermidis)의 배양일에 따른 항균효과의 지속성에 관한 연구)

  • Seo, Hyeong-Sik
    • Journal of Pharmacopuncture
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    • v.10 no.3
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    • pp.71-76
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    • 2007
  • Objectives This experimental study was performed to investigate the continuous anti-bacterial potency of Coptidis rhizoma extract on cultivation of Staphylococcus species(S. aureus, S. epidermidis) that induce eye disease. Methods Minimal inhibitory concentration(MIC) was measured by dropping to $50{\mu}l$ diluted Coptidis rhizoma extract(100%, 10%, 1%, 0.1%) on S. aureus, S. epidermidis that were cultivated from 2 to 6 days. Anti-bacterial potency was measured by the size of inhibition zone with change of volume($20{\mu}l,\;30{\mu}l,\;40{\mu}l,\;50{\mu}l$). Results 1. Anti-bacterial potency of Coptidis rhizoma extract on S. aureus was appeared in 100%, 10% and was the same as anti-bacterial potency of 2 days and 6 days. Anti-bacterial potency with change of volume(100%) was increased in propotion to increase volume on all samples. Anti-bacterial potency with change of volume(10%) was increased in propotion to increase volume on all samples except $20{\mu}l$. Anti-bacterial potency of Coptidis rhizoma extract on S. aureus was appeared continuous. 2. Anti-bacterial potency of Coptidis rhizoma extract on S. epidermidis was appeared in 100%, 10% and was the same as anti-bacterial potency of 2 days and 6 days. Anti-bacterial potency with change of volume(100%) was increased in propotion to increase volume on all samples. Anti-bacterial potency with change of volume(10%) was appeared in $50{\mu}l$. Anti-bacterial potency of Coptidis rhizoma extract on S. epidermidis was appeared continuous. Conclusions Anti-bacterial potency of Coptidis rhizoma extract on cultivation of S. aureus & S. epidermidis was showed continuous.

Effects of a Mixture of Scutellariae Radix, Coptidis Rhizoma, Coptidis Rhizoma on the Blood Pressure in Spontaneously Hypertensive Rats (황금(黃芩), 황연(黃連), 황백(黃柏) 혼합물(混合物) 전탕액(煎湯液)이 자연발증(自然發證) 고혈압(高血壓) 백서(白鼠)의 혈압(血壓)에 미치는 영향(影響))

  • Yu, Yun-Cho;Lee, Ho-Sub
    • The Journal of Korean Medicine
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    • v.20 no.2
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    • pp.141-145
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    • 1999
  • The aim of this experiment was to investigate the effects of a mixture of Scutellariae Radix, Coptidis Rhizoma and Coptidis Rhizoma water extracts on the blood pressure, plasma renin activity, plasma levels of aldosterone and atrial natriuretic peptide (ANP) in spontaneously hypertensive rats (SHR). The results of this study were as follows: 1. Systolic blood pressure decreased significantly after the administration of a mixture of Scutellariae Radix, Coptidis Rhizoma, and Coptidis Rhizoma water extract 0.2 ml/200g. 2. Plasma renin activity and plasma levels of ANP were not changed after the administration of a mixture of Scutellariae Radix, Coptidis Rhizoma and Coptidis Rhizoma water extracts, 3. Plasma levels of aldosterone was increased significantly after the administration of a mixture of Scutellariae Radix, Coptidis Rhizoma and Coptidis Rhizoma water extracts 0.1 ml/200g.

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Anti-inflammatory Effect of Coptidis Rhizoma (황련의 항염증효과)

  • Yoon, Kwang-Ro;Kim, Young-Jin;Lee, Eun;Lee, Joon-Moo
    • The Korea Journal of Herbology
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    • v.24 no.3
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    • pp.79-86
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    • 2009
  • Objectives : The present study investigated Inflammatory effect of Coptidis Rhizoma in lipopolysaccharideexposed rats and Raw 264.7 cells. Methods: The plasma concentration of IL-1$\beta$, IL-6 and TNF-$\alpha$ peaked at 5 h after LPS injection, and the values of the Coptidis Rhizoma extract groups were lower than those of the control group. In the increment of cytokines concentration at 2 h and 5 h after LPS injection, the Coptidis Rhizoma groups were lower than that of control group. The plasma concentration of IL-10 peaked at 5 h after LPS injection, and the values of the Coptidis Rhizoma extract groups were higher than those of the control group. In the increment of cytokines concentration at 2 h and 5 h after LPS injection, the Coptidis Rhizoma groups were higher than that of control group. Liver cytokines measurement was done at 5 h after LPS injection. The concentration of liver IL-1$\beta$ and IL-6 in the Coptidis Rhizoma groups was lower than that of the control group. The concentrations of liver TNF-$\alpha$, and IL-10 showed no significant differences among all the treatment groups. Results: In the studies of lipopolysaccharide-exposed Raw 264.7 cells, the concentration of IL-1$\beta$, IL-6 and TNF-$\alpha$ in the lipopolysaccharide-exposed cells groups was higher than that of control group (normal group), and in the lipopolysaccharide-exposed cells groups, these values showed a tendency to decrease in the Coptidis Rhizoma groups. The concentration of IL-10 in the lipopolysaccharide-exposed cells groups was higher than that of control group (normal group), and in the lipopolysaccharide-exposed cells groups, the values showed a tendency to increase in the Coptidis Rhizoma groups. Conclusions: These results indicate that the Coptidis Rhizoma extracts have an functional material for Inflammatory activities.

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Acute Toxicity Study on Coptidis Rhizoma in Mice (황련의 급성독성에 관한 연구)

  • 마진열;성현제;주혜정;김인락;황금희;정규용
    • Toxicological Research
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    • v.15 no.1
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    • pp.103-107
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    • 1999
  • In order to evaluate acute toxicity of Coptidis rhizoma, 6 week- and 13 week-old male ICR mice received Coptidis rhizoma extract (600~4,800 mg/kg body weight) orally, and toxicological responses were observed for consecutive 7 days. In the mice received relatively high concentration of Coptidis rhizoma($\geq$1,200mg/kg), death occurred within 3 hrs after oral administration, and its ratio in 13 week-old mice was conspicuously higher than that in 6 week-old mice. $LD_{50}$ of Coptidis rhizoma were estimated to bi 2,575 mg/kg and 1,490 mg/kg body weight in 6 week and 13 week-old mice, respectively. Coptidis rhizoma-treated animals manifested a variety of abnormal clinical findings such as ptosis, crouching, lethargy, convulsion, bizarre behavior and truning sideway. These abnormalities also ranked highly in the 13 week-old mice compared to those in the 6 week-old mice. In addition to abnormal behaviors, Coptidis rhizoma($\geq$1,200 mg/Kg) significantly elevated the urinary contents of bilirubin, urobilirubin, protein and glucose, and values in 13 week-old mice was higher than those in 6 week-old animals. No toxicological response was observed at concentration less than 600 mg/kg. Our results clearly demonstrate that susceptibility of mice to Coptidis rhizoma may be related with age, indicating that younger age mice is more resistant to the Coptidis rhizoma than the older, and toxicological mechanism of Coptidis rhizoma may be closely associated with its pharmacological mechanism.

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