• Title/Summary/Keyword: Coptidis rhizoma extract

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Evaluation of Antibacterial Effects of a Combination of Coptidis Rhizoma, Lonicerae Flos, Paeonia Japonica Extracts, and Dioctahedral Smectite Against Salmonella Typhimurium in Murine Salmonellosis

  • Jung, Won-Chul;Lee, Yeon-Ok;Cha, Chun-Nam;Lee, Yeo-Eun;Kim, Gon-Sup;Lee, Hu-Jang
    • Journal of Food Hygiene and Safety
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    • v.25 no.1
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    • pp.6-9
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    • 2010
  • The present study was undertaken to estimate the antibacterial effect of a combination of C. rhizoma, L. Flos, and P japonica (1:1:1) extracts (CLP1000) and a combination of the herbal extract mixture and dioctahedral smectite (CLPS1000) against murine salmonellosis. At the concentration of CLP1000 and CLPS1000 0.5 mg/ml, the antibacterial effect was not showed on Salmonella typhimurium (S. typhimurium). On the other hand, the antibacterial effect against S. typhimurium was observed at the concentration of CLP1000 and CLPS1000 1.0 mg/ml. Oral administration of Smectite, CLPl000, and CLPS1000 at the dose of 10 mg/ml showed a therapeutic effect for S. typhimurium infected BALB/c mice. The mortality of Smectite, CLP1000 and CLPS1000-treated mice was 90%, 90%, and 70% at 12 days, respectively, while that of untreated mice was 100% at 9 days after a lethal dose of S. typhimurium infection. The results of our study strongly indicate that CLPS1000 has potential as an effective of salmonellosis.

The Oriental medical study for Constitutions and Anticancer effect on Cancer therapy (암치료에 있어서 체질과 항암효과에 관한 한의학적 연구)

  • Sung, Hyun-Jea;Shin, Hyun-Kyu;Park, Kap-Joo;Kang, Bong-Joo;Eun, Young-Ah;Kim, Eun-Hae;Choung, Se-Young
    • Korean Journal of Oriental Medicine
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    • v.3 no.1
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    • pp.85-104
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    • 1997
  • As one of the chemical and experimental study on cancer treatment in Korea oriental medicine, we investigated the clinical characteristics on the treatment of cancer patients in oriental medical hospital. Furthermore, we carried out the cytotoxicity test of extracts of herbal medicine. The results are as follows: About Clinical Aspects of the Orient낄 Medical Cancer Treatment- (1) Clinical experience is that 420 Oriental medical hospitals were surveyed. Number of cancer patiens treated by one oriental medical doctor was under 10(about 60%)or from 10 to 20(about 30%). (2) Diagnostic Procedure Is that more than 80% of cancer patients were diagnosed by medical doctor of western hospital. (3) Therapeutic Method is that combination of oriental and western medical treatment was used in oriental medical hospital. (4) Relationship between cancer and constitutions is that to the relationship between cancer and constitutions, about 75% responded and most were considered to be Um. About Experimental Study- Toevaluate the direct cytotoxicity of hot water and methanol extracts of 34 oriental medical prescriptions on stomach cancer cell, SRB assay was carried out. (1) The $IC_{50}$ value of water extract of Samsoum was $19{\mu}g/ml$ and that of methanol extract was $15{\mu}g/ml$ so that we confirmed Samsoum has a strong direct cytotoxicity. (2) The $IC_{50}$ values of water extract of Coptidis Rhizoma, methanol extract of Caesalpiniae Lignum and Draba Semen were $10{\mu}g/ml$, $9{\mu}g/ml$ and $12.5{\mu}g/ml$ respectively, so that we confirmed Coptidis Rhizoma, Caesalpiniae Lignum and Drabs Semen have a strong direct cytototoxicity. (3) The $IC_{50}$ lalue of combinatory prescription of Rhei Rhizoma, Forsythie Fructus and Caesalpiniae Lignum was about $40{\mu}g/ml$.

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Rhei Rhizoma Mainly Blended Prescriptions According to the Fomula, Manipulation, Related Co-herb in Dongeuibogam (『동의보감(東醫寶鑑)』 중 대황(大黃)이 주약(主藥)으로 배오(配伍)된 방제(方劑)의 제형(劑形), 포제(?製), 약대구성(藥對構成)에 따른 활용(活用))

  • Joh, Hae-In;Kook, Yoon-Bum
    • Herbal Formula Science
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    • v.25 no.4
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    • pp.553-574
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    • 2017
  • The purpose of this study is to find out effects of prescriptions according to the formula, manipulation of Rhei Rhizoma, configuration. The following results were reached through investigations on the prescriptions using Rhei Rhizoma as a main component. Objectives : Analysis of prescriptions According to the formula : Liquid Extract Prescriptions were used widely on three parts to treat fever and damp heat in the interior organs. Powder Prescriptions were taken with hot water, thin porridge, tea etc. to treat damp heat, congestion of phlegm, acute episodes. Liquid Mixed Pill treat congestion of QI, damp heat, phlegm. Honey Mixed Pill treat accumulated fever, distension, acute excessive fever. Paste Pill treat blood stagnation, excessive toxic-fever, epidemic diseases. External Application treat inflammation by injury, swelling due to severe fever by internal damage. Methods : Analysis of prescriptions According to the manipulation of Rhei Rhizoma : Prescriptions including Liquor processed Rhei Rhizoma treat excessive toxic-fever, congestion of phlegm, blocking orifices on the upper side. Steamed Rhei Rhizoma strengthen effects of making evacuate and cooling of heat. Processed Rhei Rhizoma with vinegar strengthen effects of removing blood stagnation by activating blood movement, releasing gathering. Results : Analysis of prescriptions According to the Composition of Rhei Rhizoma : 41% of the total prescriptions were on the area of less than 20%. In case of lower groups show increased frequency of combination with Pharbitidis Semen, Persicae Semen, Scutellariae Radix and manipulation of baking, steaming, roasting. In case of higher groups show increased frequency of treating excess syndrome, critical illness, acute severe illness, and using proccesed Rhei Rhizoma with vinegar. Treatment of damp heat on the liver and gallbladder, disorder of the spleen and stomach is done mostly by prescriptions on the area of less than 30%. Conclusions : Rhei Rhizoma-Coptidis Rhizoma pair treat damp heat, heat toxins in blood, and Constipation caused by excessive heat. Rhei Rhizoma-Glycyrrhizae Radix pair relieve effects of Rhei Rhizoma passing blocked feces, removing the poison, activating blood movement, releasing gathering with the effects of Glycyrrhizae Radix relaxing tension by harmonizing Middle. Rhei Rhizoma-Magnoliae Cortex pair are used to treat damp heat in middle area, excessive heat in the stomach and intestine. Rhei Rhizoma-Pharbitidis Semen pair act on both blood system and QI system treating edema, damp, stagnation, heat toxins, feces. Rhei Rhizoma-Persicae Semen pair treat blood stagnation with fever on blood system.

Effects of the Coptidis Rhizoma Extract on the Membranous Nephropathy induced by Cationic Bovine Serum Albumin in Mice (황연(黃連)이 Cationic Bovine Serum Albumin 투여로 유발된 Membranous Nephropathy Mouse Model에 미치는 영향)

  • Chae, Eun-Young;Cho, Chung-Sik;Kim, Cheol-Jung
    • The Korea Journal of Herbology
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    • v.24 no.1
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    • pp.99-110
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    • 2009
  • Objectives : The current treatment regimens for patients with nephrotic syndrome due to membranous nephropathy(MN) are based on steroids or immunosuppressive therapy with the aim of reducing proteinuria and improving outcome. Although these treatments attenuate the deterioration of renal function in MN patients, it has been suggested that all are burdened by significant toxicity. Therefore, more specific and less toxic therapies are needed. This study was to evaluate the effects of Coptidis Rhizoma Extract(CRE) on the MN induced by cBSA in mice. Methods : Mice were divided into 4 groups. One group named for 'Normal' was injected with a saline solution not to be immunized. The rest groups were treated as follows; After mice were immunized with 0.2 mg of cBSA and Freund's complete adjuvant one time every two weeks for 6 weeks, they received intra-peritoneal injection of 10 mg/kg of cBSA daily for 4 weeks. Also, they were divided into 3 groups. The first named for 'Control' was not given CRE. The second for 'CRE-250' was given oral administration of 250 mg/kg of CRE daily for 4 weeks. The third for 'CRE-500' was given 500 mg/kg of CRE. All of mice were sacrificed 4 weeks after the first immunization. We measured a body weight and 24hrs proteinuria as well as serological analysis. The morphologic changes of renal glomeruli were also observed with a light microscope and an electron microscope. Results : The levels of 24 hrs proteinuria, triglyceride, IgG, IL-6 were significantly decreased in both CRE groups. And the level of IgM was significantly decreased in CRE-250 group. In histological findings of kidney tissue, thickening of GBM and deposition of electron-density were consideraly decreased in both CRE groups. Conclusions : The present study suggests that CRE is highly effective when treating mice with MN induced by cBSA. More clinical data and studies are to be done for efficient application.

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Comparative Pharmacokinetics of Berberine After Oral Administration of Pure Berberine, Coptidis Rhizoma Extract, and Decoctions of Two Different Complex Herbal Formulas to Rats

  • Chen, Jianbo;Yan, Xitao;Kim, Kyung Tae;Kim, Sang Hyuck;Lee, Eun-Sil;Lee, Young Keun;Son, Chang Gyu;Lee, Eun Hee;Park, Yong Ki;Cho, Cheong Weon;Kim, Young Ho;Byun, Youngjoo;Kang, Jong Seong
    • Bulletin of the Korean Chemical Society
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    • v.34 no.5
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    • pp.1559-1562
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    • 2013

Protective Effects of BJS-mix001, in Indomethacin induced Gastric Damages in Rats (BJS-Mix001이 Indomethacin 유발 랫트 위점막 손상에 미치는 영향)

  • Lim, So-Yeon;Byun, Joon-Seok;Kim, Dae-Jun;Kwak, Min-A
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.26 no.2
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    • pp.181-188
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    • 2012
  • The object of this study was to observe the gatro protective effects of BJS-mix001, a mixed herbal formula consisted of 4 herbal drugs Pinelliae Rhizoma : Coptidis Rhizoma : Massa Medicata Fermentata : Ostreae Testa = 1 : 1 : 1 : 1 (g/g) mixtures, which were main component of oriental medicine for treating various digestive diseases, in indomethacin induced gastric damages in rats. Three different dosages of BJS-mix001 (200, 100 and 50 mg/kg) were once orally administered 30 min before indomethacin treatment. Six hrs after indomethacin treatment, changes on the gross lesion scores, fundic histopathology, MPO activity and anti oxidant activities were observed. The results were compared with omeprazole, antioxidant and proton pump inhibitor 10 mg/kg and DA-9601, a standardized extract of the herb Artemisia asiatica 100 mg/kg treated group, respectively. As results of all three different dosages of BJS-mix001 in the indomethacin induced gastric damaged rats, significant decreased gastric damages were detected as compared with the indomethacin treated control rats. BJS-mix001 also strengthened the antioxidative defense systems - decreased the level of lipid peroxidation and catalase activity but increased the level of superoxide dismutase and glutathione contents. BJS-mix001 showed similar gastro protective effects as compared with equal dosage of DA-9601, and BJS-mix001 50 mg/kg showed slighter effects as compared with omeprazole 10 mg/kg, in the present study. The results obtained in this study suggest that BJS-mix001 showed favorable effects in the indomethacin induced gastric damages mediated by strengthening of the antioxidative defense systems.

Effect of Samhwangsashimtang Extract on Lipopolysaccharide-Stimulated Inflammatory Response and Macrophage Activity (삼황사심탕(三黃瀉心湯) 추출물의 lipopolysaccharide 유도에 의한 염증 조절과 대식세포 활성에 대한 연구)

  • Kang, Hee;Kwon, Han-Al;So, Hyung-Jin;Lee, Jeong-Min;Lew, Jae-Hwan;Choi, Ho-Young
    • The Korea Journal of Herbology
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    • v.27 no.6
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    • pp.23-28
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    • 2012
  • Objectives : Samhwangsashim-tang(SHSST), a mixture of Rhei radix et rhizoma, Scutellariae radix, and Coptidis rhizoma, has been regarded as being able to treat bleeding, gastric discomfort, dry mouth, insomnia and purpura due to Blood Heat. Currently, this herbal formula is applied to gastritis, gastric ulcer, hypertension, atherosclerosis or other types of vascular inflammatory disorders. Methods : We extracted this herbal mixture with 30% ethanol and examined for its effects on systemic inflammatory responses and in vitro macrophage activity. Mice were orally given to SHSST for 7 days and then lipopolysaccharide(LPS) was intraperitoneally injected. Tumor necrosis factor-${\alpha}$(TNF-${\alpha}$) levels in serum were measured 1 h after LPS challenge. Peritoneal macrophages were isolated from thioglycollate-injected mice and used for in vitro cellular activity. Cell death was measured using the MTT method and annexin V/propidium iodide staining. LPS-stimulated signaling molecules necessary for TNF-${\alpha}$ expression were determined by Western blotting. Results : Oral administration of SHSST for 7 days resulted in a significant reduction in LPS-stimulated TNF-${\alpha}$ release into serum. In vitro treatment of SHSST was cytotoxic in a concentration-dependent manner. However, SHSST caused a concentration-dependent reduction in necrosis and increase in apoptosis in mouse peritoneal macrophages. SHSST inhibited the activation of NF-${\kappa}B$, p38 and JNK signaling molecules in response to LPS. Conclusion : Taken together, our results demonstrated that SHSST was effective in lowering LPS-stimulated TNF-${\alpha}$ serum levels, possibly through its modulation of NF-${\kappa}B$, p38 and JNK in macrophages.

Ameliorative Effects of Hwangnyeonhaedok-tang on Atopic Dermatitis (황련해독탕(黃連解毒湯)의 아토피 피부염 개선 효과(效果))

  • Ki, Ho-Pil;Jang, Seon Il;Yun, Young-Gab
    • Herbal Formula Science
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    • v.21 no.1
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    • pp.80-90
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    • 2013
  • Objectives : The water extract of Hwangnyeonhaedok-tang (HHT), composed of the Scutellariae Radix, Coptidis Rhizoma, Phellodendri Cortex and Gardeniae Fructus has been traditionally used to treat fever, inflammation, gastritis and hypertension in east asia. However, little is known about the ameliorative effects of HHT on atopic dermatitis. Therefore, the purpose of this study was to investigate the therapeutic effect of HHT on atopic dermatitis Methods : We investigated the inhibitory effects of HHT on the production of proinflammatory cytokines in rat peritoneal mast cells (RPMCs), on the scratching behavior in ICR mice, and on atopic dermatitis symptoms in 2,4-dinitrofluorobenzene (DNFB)-induced atopic dermatitis-like model hairless mice. Results : Levels of TNF-${\alpha}$ and IL-$1{\beta}$ increased by PMA plus A23187 co-treatment were significantly inhibited by HHT in a dose-dependent manner. HHT also inhibited the histamine release from RPMCs stimulated by compound 48/80, which promotes histamine release. The oral administration of HHT reduced the scratching behavior induced by compound 48/80 and histamine in ICR mice. Furthermore, the intradermal treatment of HHT reduced the ear edema, skin lesions, and atopic molecular marker (IgE and IL-4) in DNFB-induced atopic dermatitis model mice. Conclusions : These results suggest that HHT may be used as a potential treatment for AD as a prescription for treatment of atopic dermatitis.

Neuroprotective Effects of Berberine in Neurodegeneration Model Rats Induced by Ibotenic Acid

  • Lim, Jung-Su;Kim, Hyo-Sup;Choi, Yoon-Seok;Kwon, Hyock-Man;Shin, Ki-Soon;Joung, In-Sil;Shin, Mi-Jung;Kim, Yun-Hee
    • Animal cells and systems
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    • v.12 no.4
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    • pp.203-209
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    • 2008
  • Berberine, an isoquinoline alkaloid found in Coptidis Rhizoma(goldenthread) extract, has multiple pharmacological effects such as anti-inflammatory, antimicrobial and anti-ischemic effects. In the present study, we examined the effects of berberine on neuronal survival and differentiation in a hippocampal precursor cell line and in the memory deficient rat model. Berberine increased in a dose dependent manner the survival of hippocampal precursor cells as well as differentiated cells. In addition, berberine promoted neuronal differentiation of hippocampal precursor cells. In the memory deficient rat model induced by stereotaxic injection of ibotenic acid into entorhinal cortex(Ibo model), hippocampal cells were increased about 2.7 fold in the pyramidal layer of CA1 region and about 2 fold in the dentate gyrus by administration of berberine after 2 weeks of ibotenic acid injection. Furthermore, neuronal cells immunoreactive to calbindin were increased in the hippocampus and entorhinal cortex area by administration of berberine. Taken together, these results suggest that berberine has neuroprotective effect in the Ibo model rat brain by promoting the neuronal survival and differentiation.

Antimicrobial Effect of Medicinal Plants against Methicillin-Resistant Staphylococcus aureus (MRSA) (약용식물의 항생제 내성균주에 대한 항균활성)

  • Ji, Young-Ju;Lee, Ji-Won;Lee, In-Seon
    • Journal of Life Science
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    • v.17 no.3
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    • pp.412-419
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    • 2007
  • In the present study, we investigated antimicrobial activity of the medicinal plants against various strains of methicillin-resistant Staphylococcus aureus (MRSA) and Staphylococcus aureus (S. aureus). Among the tested, the plants extracts of Asiasarum heterotropoides var. mandshuricum, Coptidis rhizoma, Reynoutria elliptica Migo., Solidago virga-aurea var. gigantea Miq.seed exhibited significant antimicrobial activities against MRSA KCCM 11812, 40510 and S. aureus ATCC 25923. The methanol extract of Asiasarum heterotropoides var. mandshuricum showed strong antimicrobial activity against MRSA KCCM 11812, 40510 and S. aureus ATCC 25923 at the 5 mg/disc. A synergistic effect was found in combined extracts of Asiasarum heterotropoides var. mandshuricum and Coptidis rhizoma as compared to each extracts alone. The result suggests that medicinal plant extracts can be used as an effective natural antimicrobial agent in food.