• Microbiology and Biotechnology Letters
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• v.2 no.2
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• pp.103-109
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• 1974

In the cource of the studies on L-glutamic acid production from acetic acid, 383 strains capable of assimilating acetate as sole source of carbon were isolated from 279 kinds of soil sample. Out of them, 5 strains which produced relatively larger amount of L-glutamate from acetate were selected and named Brevibacterium flavum nov. sp. D1005B, Corynebacterium glutamicum nov. sp. D1025A, Brevib. flavum nov. sp. D2209B, Coryneb. acetoacidophilum nov. sp. D2212B and Coryneb. acetoacidophilum nov. sp. D2349A respectively.

• Biotechnology and Bioprocess Engineering:BBE
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• v.9 no.4
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• pp.326-329
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• 2004

Corynebacterium acetoacidophilum RYU3161 was cultivated in a L-histidine-limited fed-batch culture. To investigate the effect of cell growth on the L-proline production, 5 L fed-batch culture was performed using an exponential feeding rate to obtain the specific growth rates $(\mu)$ of 0.04, 0.06, 0.08, and 0.1 $h_1$. The results show that the highest production of L-proline was obtained at $\mu$ = 0.04 $h_1$. The specific L-proline production rate $(Q_p)$ increased pro-portionally as a function of the specific growth rate, but decreased after it revealed the maxi-mum value at $\mu$ = 0.08 $h_1$. Thus, the highest productivity of L-proline was 1.66 g $L^-^1 h^-^1$ at $\mu$ = 0.08 $h_1$. The results show that the production of L-proline in C. acetoacidophilum RYU3161 has mixed growth-associated characteristics.

• 한국생물공학회:학술대회논문집
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• 2001.11a
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• pp.426-429
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• 2001

The continuous production of L -proline was studied using L-histidine auxotrophic mutant of Corynebacterium acetoacidophilum under various substrate limited conditions. Among the $NH_4\;^+$ $PO_4\;^3$ and L -histidine limited conditions, the highest production of L -proline was observed under the L-histidine limited condition. Under $NH_4\;^+$ and $PO_4\;^3$ limited conditions, no or poor L-proline production was observed, respectively. For the kinetic parameters under L -histidine limitation the specific rate of L -proline production was increased with dilution rate upto $0.1hr^{-1}$ but decreased above $0.1hr^{-1}$. The volumetric rate of L -proline production was showed similar pattern with specific rate. The dried cell weight was gradually increased according to decrease the dilution rate. Specific rate of glucose consumption was proportionally increased with dilution rate. The results of continuous culture (higher production of L-proline at dilution rate $0.1hr^{-1}$) will be used in fed-batch culture for the control of cell growth rate and mass production of L-proline.

• Journal of Microbiology and Biotechnology
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• v.9 no.5
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• pp.613-618
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• 1999

L-Proline-producing mutant strains were developed by exposing L-glutamic acid-producing bacteria to N-metyl-N-nitro-nitrosoguanidine and UV irradiation. A L-histidine auxotroph of Corynebacterium acetoacidophilum RYU3161(KCTC 0616BP), which was resistant to sulfaguanidine and proline analogs (DHP, AZC, TAC), was isolated. The activity of the mutant strain's $\gamma$-glutamyl kinase was 45% higher than that of the parent strain. The optimum level of L-histidine for production of L-proline was 0.16 g/l. In a 5-1 jar fermenter, the mutant strain produced L-proline at a high concentration (35 g/l) level within 48 h of cultivation.