• Journal of Environmental Science International
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• v.20 no.10
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• pp.1265-1272
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• 2011

In this study, the extraction characteristics of soluble solid from Rumex crispus(Curled dock) was studied from the investigation of the effects of experimental conditions on extraction rate; extraction ratio, composition of extractants, extraction time and pH of extractant, etc. The proximate composition of Rumex crispus was 2.58% crude lipid, 5.59% crude protein, 7.39% crude ash, 6.13% moisture and 78.31% carbohydrate, respectively. Turbidity of extract by distilled water was higher and increased with extraction time and extraction temperature, where as the turbidity didn't increase by ethanol and methanol in 20 folds of extraction ratio. Turbidity was inversely proportional to the extraction ratio for the three extractants at 25$^{\circ}C$ and 1 hour extraction. But turbidity of extract was highest by composition of 50% methanol-water extractant than any other compositions of extractants. Eighteen and fifteen free aminoacids were detected in extracts with distilled water, methanol and ethanol extractant, respectively, and it's contents were order of glutamic acid>proline>aminobutyric acid>alanine. The extraction rate of soluble solid from Rumex crispus was order of distilled water>methanol>ethanol within experimental extraction ratio. In extraction with distilled water, the contents of soluble solid was inversely proportional to the pH of extractant.

• Journal of The Korean Society of Grassland and Forage Science
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• v.38 no.4
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• pp.325-330
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• 2018

This study was carried out to assess the composting temperature effects on germination of curled dock and barnyard millet seeds. After the seeds wrapped in gauze were buried in the compost heap, the seeds were taken out under monitering the temperature for measuring germination rate at 1.5, 3, 10 and 20 days of composting. The germination rate of the seeds was directly related to the temperature of the compost heap regardless of the type of composting method. The seeds that buried in the compost for 1.5 days at a temperature of $60{\sim}70^{\circ}C$ were completely lost their germination ability. However, the germination rate of the seeds retained about 10% even after 20 days when compost temperature was below $51^{\circ}C$. Reulsting data suggests that, it is necessary to keep the compost temperature at least $55^{\circ}C$ for more than 3 days in order to suppress the germination of the seeds. On the other hand, if the temperature of the compost rises by $60{\sim}70^{\circ}C$, the seeds will lose their gemination ability with in 1.5 days. In conclusion, aerobic composting would be more effective in suppressing germination ability of curled dock seed and barnyard millet seeds.

• YAKHAK HOEJI
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• v.57 no.6
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• pp.406-411
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• 2013

Rumex crispus (Curled Dock, Polygonaceae) is a perennial wild plant used in traditional medicine as a laxative, astringent, and to treat blood and skin disease. The ethanol extract of R. crispus was obtained and its carbohydrate contents were analyzed using high-performance anion-exchange chromatography with pulsed amperometric detection. The anabolic effects of R. crispus in human osteoblastic MG-63 cells were investigated using the WST-8 assay, alkalinephosphatase (ALP) assay, and mineralization assay. The ethanol extract increased the proliferation of MG-63 cells and stimulated ALP activity in a dose-dependent manner over a 72-hrs period. Additionally, the ethanol extract dose-dependently stimulated the formation of bone nodules in MG-63 cells treated for 12 days. The ethyl acetate fraction from the ethanol extract did not affect osteoblast viability but induced an increase in ALP activity. In conclusion, the ethanol extract of R. crispus increases the proliferation and bone-forming activity of osteoblasts, and hence it could be used in the development of bone-forming stimulatory nutraceuticals and osteoporosis-related medicines.

• Korean Journal of Plant Tissue Culture
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• v.26 no.1
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• pp.7-14
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• 1999

In order to survey anthraquinone productivity from in vitro root culture, transformed hairy roots of Rumex crispus were induced from leaf segments by infection with Agrobacterium rhizogenes strain $A_4$ and compared with adventitious roots. The optimum condition of adventitious root formation from leaf segments was 5 $\mu$M NAA added to MS medium. Mannopine was detected in the extract of hairy roots by paper electrophoresis, but not in adventitious roots. Secondary root tips of both adventitious roots and hairy roots elongated without lateral root branching in hormone free MS medium, but primary root tips showed more rapid growth with extensive lateral root branching. MS basal medium was the best for growth of the adventious roots and hairy roots for anthraquinone content. Adventitious root tips and hairy root tips cultured in liquid MS medium supplemented with 0.05 $\mu$M NAA and 0.1 $\mu$M kinetin (contained 5% sucrose) showed the maximal growth and anthraquinone content. Anthraquinone content of hairy roots was increased by the culture periods, but was reduced after 25 days of culture.

• Asian Journal of Turfgrass Science
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• v.20 no.2
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• pp.157-165
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• 2006

This study was initiated to investigate herbicidal selectivity margin with annual bluegrass and bentgrass according to growth stages through herbicidal spectrum of bispyribac-sodium and therefore to examine application possibility to prevent annual bluegrass selectively. The herbicidal efncacy by pre-emergence application was generally low. But a high herbicidal efficacy was observed over 90% for Shehpherd's purse. Foliar treatment resulted in a higher herbicidal efficacy in all tested plants except dallisgrass, lovegrass, curled dock and bentgrass as compared with pre-emergence application. We found bispyribac-sodium could be used as a selective weeding chemical between annual bluegrass and bentgrass. In regard to dose-responses to the growth stage, annual bluegrass was more susceptible to bispyribac-sodium than bentgrass for the same dosage. Particularly, bispyribac-sodium application in inflorescence emergence stage showed a greater herbicidal selectivity margin of 6.8 times with annual bluegrass over creeping bentgrass after 28 days from the application. These data demonstrated that annual bluegrass was more sensitive to bispyribac-sodium over creeping bentgrass.

• YAKHAK HOEJI
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• v.58 no.5
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• pp.307-313
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• 2014

Rumex crispus (curled dock), which is a perennial wild plant, has long been used as a laxative, astringent, and medicine to treat blood and skin diseases. We recently reported that the roots of R. crispus are an effective nutraceutical for bone. This study prepared ethanol extracts of the leaves and roots of R. crispus, and analyzed the major constituents using liquid chromatography and mass spectrometry. In addition, their effects on the proliferation and differentiation of human osteoblast-like MG-63 cells, such as cell viability, alkaline phosphatase (ALP) activity, collagen content, and mineralization, were compared. The chromatograms of the chemical constituents of the two extracts exhibited quite different profiles: quercetin and quercitrin were identified as major peaks in the leaf extract, whereas cinnamtannin B1 and procyanidin isomers were the major peaks for the root extract. Neither extract was cytotoxic at concentrations of < $25{\mu}g/ml$. ALP activity and collagen synthesis-which are markers of the early stage of osteogenesis-in MG-63 cells were significantly increased upon the addition of the root extract compared with the addition of the leaf extract. In contrast, the leaf extract had a more stimulatory effect on mineralization-which is marker of the late stage of osteogenesis-in MG-63 cells than did the root extract. In conclusion, extracts of both leaves and roots of R. crispus stimulated the bone-forming activity of osteoblasts; in particular, the root extract was more effective in the early stage of osteoblast differentiation, while the leaf extract was more effective in the late stage. This difference in anabolic activity may be due to differences in the constituents of the leaves and roots. The leaves and roots of R. crispus appear to complement each other as stimulators of bone formation.