• Title/Summary/Keyword: Cyprinus carpio

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Experimental Infection of Clonorchis sinensis to Cyprinus curpio nudes (간흡충의 이스라엘잉어(향어)에 대한 감염실험)

  • 이재구;김평길
    • Parasites, Hosts and Diseases
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    • v.21 no.1
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    • pp.11-18
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    • 1983
  • As a part of observation on the suitability as a second intermediate host of Clonorchis sinensis, various infection experiments were tried to Cyprinus carpio nodus. The results obtained were summarized as follows: 1. The cercariae which attempted to contact with Cyprinus carpio nodus in the water were observed under the stereomicroscope. After contact, cercariae began to separate its tails from the bodies at 4 minutes increasingly, and then the numbers ranged to 80% at 13 minutes after the encounter. But very few cercariae could actually invade into the epidermis of the fish. 2. When Cyprinus carpio nudes were exposed to a number of cercariae in the beaker, only a few cercariae could invade through the epidermis. Most of the invaded cercariae were killed before forming the cyst. Also rare encysted cercariae were found to be dead within 48 hours. 3. Cyprinus carpio nudes were reared with Parafossarulus manchouricus shedding numerous cercariae in the indoor aquarium. When observed after 12 days, to determine the susceptibility, no metacercaria was found infected. 4. Cyprinus carpio nudum were reared with Parafossarulus manchouricus shedding numerous cercariae in Kimhae lake for 2 months in summer of 1982, in order to check the natural infection. No metacercaria of Clonorchis sinensis was found from the fish. 5. In the cases of control fish, Pseudoraibora larva, numerous cercariae were inspected on the course of invasion through epidermis of the fish under the stereomicroscope. And many metacercariae were also found from the Ssh whenever they were kept in beaker, indoor aquarium, or the lake for natural infection. The results suggested that Cyprinus carpio kudus might not be proper intermediate host of Clonorchis sinensis.

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Cytogenetic Analysis of Induced Hybrid between Common Carp (Cyprinus carpio) and Crucian Carp (Carassius auratus) (잉어(Cyprinus carpio)와 붕어(Carassius auratus)간 잡종의 세포유전학적 분석)

  • 남윤권;오승용;조재윤;김동수
    • Journal of Aquaculture
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    • v.11 no.1
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    • pp.77-81
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    • 1998
  • Cytogenetic analyses were carried out with induced hybrid between common carp (Cyprinus carpio) female and crucian carp (Carassius auratus) male. The erythrocytic measurement revealed that cellular and nuclear size of induced hybrids were intermediate between those of paremtal species. The modal chromosome numbers of common carp, crucian carp and its hybid were same as 2n=100. The DNA content of induced hybrids determined based on flow cytometry was 3.7pg/cell which corresponding to intermediate value between the carp (3.6ph/cell) and crucian carp (3.8pg/cell)

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An Enzyme-Linked Immunosorbent Assay for Carp (Cyprinus carpio) Vitellogenin and Assay for Oestrogenic Chemicals

  • Jeong, Jing-Woon;Park, Eon-Jung;Kim, Andre;Park, Jang-Su;Park, Heung-Jai
    • Proceedings of the Korean Environmental Sciences Society Conference
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    • 2001.05a
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    • pp.238-239
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    • 2001
  • The egg-yolk precursor vitellogenin(VTG) is secreted by the liver of female and male fish, in response to estrogenic compound. Carp(Cryprinus carpio) vitellogenin of one major protein is 160kDa, two minor proteins is 110kDa and 170kDa. We were induced vitellogenin by inject of 17-estradiol and purified in a two step procedure by separating it from plasma protein precipitated by 35% saturated ${(NH_4)}_2SO_4$ and then from the remainder by Mono-Q chromatography. We found major carp(Cyprinus carpio) vitellogenin band at 160kDa. Effect of different concentration of oestrogen on vitellogenin synthesis in carp(Cyprinus carpio) exposed for 4 weeks. Show differential effects on vitellogenin synthesis 7 days after treatment. Plasma vitellogenin was measured 3 times for each by an enzyme linked immunosorbent assay(ELISA). ELISA was developed for the detection of the egg yolk precursor vitellogenin in plasma of carp(Cyprinus carpio). The ELISAS performance was optimized and characterized.

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First report on Myxobous artus infection in leather carp (Cyprinus carpio nudus) in Korea (향어(Cyprinus carpio nudus)의 Myxobolus artus 국내 첫 감염사례 보고)

  • Jun-Young Song;Ahran Kim
    • Journal of fish pathology
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    • v.36 no.2
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    • pp.409-414
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    • 2023
  • Ellipsoidal-shaped spores with two polar capsules were detected in leather carp (Cyprinus carpio nudus) muscle. 18S rDNA gene analysis of the spore showed a 99.58% match to Myxobolus artus, a myxozoan parasite. As a result of phylogenetic analysis using the Bayesian inference model and maximum likelihood model among other Myxobolus species, the isolate in the present study belonged to the M. artus cluster. This is the first case report of M. artus infection detected in domestic aquaculture organisms in Korea.

Monitoring of carp edemavirus disease and herpesviral hematopoietic necrosis in Cyprinus carpio and Carassius auratus imported from China (중국에서 수입된 잉어 및 붕어의 잉어부종바이러스병 및 허피스바이러스성 조혈기괴사증 모니터링)

  • Kwon, Se Ryun
    • Journal of fish pathology
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    • v.33 no.1
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    • pp.1-6
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    • 2020
  • In this study, carp edemavirus disease and herpesviral hematopoietic necrosis were examined in carp (Cyprinus carpio) and crucian carp (Carassius auratus) imported from China. From August 2016 to May 2017, 100 carp and 300 crucian carp were examined. There were no clinical symptoms in all the tested fish, and no internal or external parasitic infections were identified except for the detection of Argulus sp. in one leather carp. Carp edema virus disease was examined by PCR in C. carpio, but all were negative. In C. auratus, herpesviral hematopoietic necrosis was tested by PCR and 4 out of 300 were positive. The results of this study are expected to be useful as basic data for conducting risk analysis on the relevant organisms and counterpart countries or for designating new pathogens for quarantine.

Effects of Dietary Lipid and Paprika Levels on Growth and Skin Pigmentation of Red- and White-colored Fancy Carp Cyprinus carpio var. koi (사료의 지질 및 파프리카 함량이 비단잉어(Cyprinus carpio var. koi) 홍백 치어의 성장 및 체색에 미치는 영향)

  • Kim, Yi-Oh;Lee, Sang-Min
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.45 no.4
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    • pp.337-342
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    • 2012
  • A feeding trial was conducted to investigate effect of three paprika levels (0%, 5% and 10%) with two lipid levels (4% and 10%) in diets on growth performance and skin coloration of red- and white-colored fancy carp, Cyprinus carpio var. koi. Two replicate groups (an average body weight of $4.3{\pm}0.2g$) of fish fed one of eight experimental diets for 8 weeks. Survival, weight gain and feed efficiency of the fish were not significantly affected by both dietary paprika and lipid levels (P>0.05). After 8 weeks feeding period, the values of $a^*$, $L^*$ and $b^*$ of fish skin were significantly changed by dietary paprika level (P<0.05) but not lipid level. The $a^*$ value of fish fed the diets containing paprika was higher than that of fish fed control diet (P<0.05). Additional inclusion of 50-100 ppm canthaxanthin in diets containing 5% and 10% paprika did not improve $a^*$ value of fish skin. Based on the results of this study, it can be concluded that dietary inclusion of 5% paprika powder could increase the redness of red- and white-colored fancy carp fingerling.

Influence of Dietary Lipid Level and Feeding Frequency on the Growth and Body Composition of Juvenile Fancy Carp Cyprinus carpio var. koi (사료의 지질함량과 공급횟수가 비단잉어(Cyprinus carpio var. koi) 치어의 성장 및 체조성에 미치는 영향)

  • Kim, Yi-Oh;Lee, Sang-Min
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.50 no.6
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    • pp.745-749
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    • 2017
  • We investigated the effects of different dietary lipid levels (7% or 14%) and feeding frequency (1-4 meals per day) on the growth performance and body composition of juvenile fancy carp Cyprinus carpio var. koi. Three replicate groups of fish (initial mean weight, 11.7 g) were fed to visual satiety with sinking dry pellet diets for 8 weeks. Neither feeding frequency nor lipid level affected fish survival. Weight gain was affected by feeding frequency but not dietary lipid level. Weight gain significantly increased with increased feeding frequency. Feed efficiency and protein efficiency ratio were affected by feeding frequency, but not dietary lipid level. Daily feed intake was affected by feeding frequency and dietary lipid level. Whole body moisture, crude lipid and ash contents were affected by feeding frequency, but not dietary lipid level. Based on these results, we conclude that the appropriate feeding frequency for the optimum growth of fancy carp fingerlings is four meals per day using sinking dry pellet.

Dual effect of Low- frequency Electromagnetic Field on Muscle Histopathology of Caspian Sea Cyprinus carpio

  • Samiee, Farzaneh;Samiee, Keivandokht
    • Journal of fish pathology
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    • v.30 no.1
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    • pp.51-61
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    • 2017
  • The effect of electromagnetic field on aquatic organisms has received little attention. In the current study, the effect of 50Hz electromagnetic field on muscle histopathology of Caspian Sea Cyprinus carpio, a species of economic importance, was investigated. A total of 120 healthy fish were used in this study. They were classified randomly in one of two groups as follows: Control or unexposed EMF group and experimental group with 5 different magnetic field intensities (0.1, 1, 3, 5 and 7mT) at 2 different exposure times including 30 and 60 minutes. Fish in the experimental group were exposed only once. Two weeks after exposure, dorsal muscles sectioned transversely, stained and were examined using a light microscope. Histopathologic assessments showed significant difference between control and EMF exposed groups at both 30 min. (p<0.01) and 60 min. (p<0.001) exposure times. We report for the first time that electromagnetic field in interaction with muscular tissue of Cyprinus carpio exhibits a dual effect which depends on the field intensity, and exposure time. At short exposure time (30 min.), EMF stimulates muscle growth process. At longer exposure time (60 min.), EMF can damage muscle tissue and result in muscle necrosis. More research is required to elucidate precise mechanisms involved in muscle hypertrophy and pathologic changes.

A case of Aeromonas veronii infection in Israeli carp (Cyprinus carpio): phylogenetic analysis and antimicrobial resistance (이스라엘 잉어(Cyprinus carpio)에서 Aeromonas veronii 감염증의 증례: phylogenetic analysis와 항생제 내성)

  • Yi, Seung-Won;You, Myung-Jo;Lee, Hae-Beom;Shin, Gee-Wook
    • Korean Journal of Veterinary Service
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    • v.35 no.3
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    • pp.239-243
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    • 2012
  • We reported an outbreak of Aeromonas (A.) veronii responsible for ulcerative dermatitis in Israeli carp (Cyprinus carpio). The major clinical signs were darkening body, abdominal extension, exophthalmos and severe ulcerative necrosis in the skin. The necropsy showed yellowish ascites, necrosis in liver and enlargement of kidney and spleen in the morbid fish. In blood agar for culturing bacteria, three different colonies were identified as A. veronii, Plesimonas shigelloides and Shewanella putrefaciens by phylogenetic identification using 16S rRNA or gyrB gene sequences. A. veronii was the most dominant species among them and was resistant to ampicillin, nalidixic acid and oxytetracycline.