• Title/Summary/Keyword: Cytotoxicity

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Cytotoxicity of Copper Nanoparticles in Cultured Human Bronchial Epithelial Cells (BEAS-2B) (구리로 만든 나노입자의 기관지상피세포에 미치는 독성)

  • Park Eun-Jung;Park Kwangsik
    • Toxicological Research
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    • v.21 no.4
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    • pp.303-307
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    • 2005
  • Nanomaterials, which ranges in size from 1 to 100 nm, have been used to create uqnique devices at the nanoscale level possessing novel physical and chemical functional properties. However, the toxicities of nanomaterials have not been fully tested and the risk of nanomaterials is emerging issues in these days. In this study, the cytotoxicity of copper nanoparticles was tested in cultured human bronchial epithelial cells. As a results, copper nanoparticles showed cytotoxicity similar with cupric ion and the apoptotic mechanisms of DNA fragmentation and caspase-3 activation were involved. Induction of heme oxygenase-1 and thioredoxin reductase by copper nanoparticles indicated that cytotoxicity of copper nanoparticles is likely to be mediated through oxidative stress.

Protective Effect of Crataegus pinnatifida and Cinnamomum cassia on Ethanol-induced Cytotoxicity and DNA Damage in HepG2 Cells

  • Kim, Nam Yee;Song, Eun Jeong;Heo, Moon Young
    • Natural Product Sciences
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    • v.20 no.4
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    • pp.237-242
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    • 2014
  • Plant extracts produced from branches of Crataegus pinnatifida and barks of Crataegus pinnatifida inhibited ethanol-induced cytotoxicity and DNA damage in liver cells. Furthermore, these two extracts inhibited the expression and activities of CYP2E1 enzyme. Cinnamomum cassia had a better effect on inhibition of DNA damage than Crataegus pinnatifida, as well as showed a high tendency to inhibit CYP2E1 expression and catalytic activities. It is considered that extracts produced from Crataegus pinnatifida or Cinnamomum cassia have an effect to reduce ethanol-induced cytotoxicity and DNA damage in liver cells. Therefore, we suggest to use Crataegus pinnatifida and Cinnamomum cassia and their ingredients as potential candidate substances to prevent and treat ethanol-induced cytotoxicity and genotoxicity in liver cells.

Asparagus cochinchinensis inhibits the ethanol-induced cytotoxicity in Hep G2 cells

  • Kim, Jeong-Joong
    • Advances in Traditional Medicine
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    • v.1 no.1
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    • pp.89-96
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    • 2000
  • A human hepatoma cell line, Hep G2 cells are a reliable for the study of alcohol-induced hepatotoxicity. In this study, the author investigated the effect of an aqueous extract of Asparagus $cochinchinensis_{MERRIL}$ (Liliaceae) roots (ACAE) on ethanol (EtOH)-induced cytotoxicity in Hep G2 cells. ACAE dose-dependently inhibited the EtOH-induced tumor necrosis $factor-{\alpha}\;(TNF-{\alpha})$ secretion. ACAE also inhibited the EtOH and $TNF-{\alpha}-induced$ cytotoxicity. Furthermore, the author found that ACAE inhibited the $TNF-{\alpha}-induced$ apoptosis of Hep G2 cells. These results suggest that ACAE may prevent the EtOH-induced cytotoxicity through inhibition of the apoptosis of Hep G2 cells.

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Synthesis, Cytotoxicity and Topoisomerase II Inhibitory Activity of Benzonaphthofurandiones

  • Rhee, Hee-Kyung;Kwon, Young-Joo;Chung, Hwa-Jin;Lee, Sang-Kook;ParkChoo, Hea-Young
    • Bulletin of the Korean Chemical Society
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    • v.32 no.7
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    • pp.2391-2396
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    • 2011
  • Benzonaphthofurandiones containing four coplanar fused aromatic rings were synthesized and evaluated for their cytotoxicity against five human cancer cell lines, and their inhibitory activity on topoisomerases. These benzonaphthofurandiones were prepared by condensation of 2,3-dichloronaphthoquinone and three aromatic diols with base catalysts in alcohol. The synthesized compounds were o-alkylated with six dialkylaminoalkyl halides. The hydroxy derivatives (8a-8g) exhibited relatively potent cytotoxicity among the prepared compounds. These compounds were evaluated as excellent inhibitors against topoisomerase II (topo II). Especially, the hydroxy analogue with branched methyl side chain (8e) showed high cytotoxicity against cancer cell lines and good inhibitory activity on topo II.

Screening on the Cytotoxicity of Medicinal Plants against L1210 and HL60 Cancer Cells (L1210과 HL60 암세포에 대한 야생식물의 세포독성 검색)

  • Lee, Jun-Sung;Min, Byung-Sun;Bae, Ki-Hwan
    • Korean Journal of Pharmacognosy
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    • v.27 no.3
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    • pp.173-177
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    • 1996
  • For the search of anticancer compounds from natural products, 43 plants were extracted with benzene and methanol, separately, and the extracts were screened for the cytotoxicity against L1210 and HL60 cancer cell lines. From the results, 22 samples in benzene extracts showed cytotoxicity against L1210 cells and 23 samples against HL60 cells, respectively. However, any methanol extracts did not exhibit cytotoxicity against two cancer cell lines, it suggested that cytotoxic compounds seemed to have low polarity. $ED_{50}$ values less than $5\;{\mu}g/ml$ were observed in 14 and 9 samples in benzene extracts against L1210 and HL60 cancer cells, respectively.

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Cytotoxic and Cytotoxicity-Potentiating Effects of the Curcuma Root on L1210 Cell

  • Ahn, Byung-Zun;Lee, Jeong-Hyung
    • Korean Journal of Pharmacognosy
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    • v.20 no.4
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    • pp.223-226
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    • 1989
  • A cytotoxic sesquiterpene against L1210 cell has been isolated from the root of Curcuma domestica. Its structure was identified as ${\beta}-sesquiphellandrene$. The cytotoxicity-potentiating substance was (+)-ar-turmerone. (+)-ar-Turmerone potentiated the cytotoxicity of ${\beta}-sesquiphellandrene$(5 fold in $ED_{50}$ value) and an unknown sesquiterpene which was isolated from the root as well, and that of aurapten(6.3 fold) isolated from the unripe fruit of Poncirus trifoliata. Moreover, it potentiated the cytotoxic activities of MeCCNU 10 fold and cyclophosphamide 10 fold. Except the fact that all the effective cytotoxic substances possess relatively good lipophilicity, no relationship between structures of the cytotoxic substances and the cytotoxicity-enhancing effect of (+)-ar-turmerone could be observed.

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Comparison of Eye Irritation Potency with Skin Irritation and Cytotoxicity Potency of Anti-wrinkle Agents (주름방지용 화장품원료의 안점막 자극성과 피부자극성 및 세포독성과의 비교)

  • 이은희;이종권;김주환;정경미;정해관;이선희;정수연;홍진태
    • Toxicological Research
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    • v.17 no.2
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    • pp.143-149
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    • 2001
  • In the present study, we examined eye irritation oj six anti-wrinkle agents (ascorbic acid, glycolic acid, all trans-retinoic acid, ginseng extract, retinol, EB). We also compared eye irritation with skin irritation and cytotoxicity in HaCaT cells by these agents. The highest eye irritation was found in glycolic acid, but all trans-retinoic acid showed the highest skin irritation. The rank of eye irritation was not correlated with the cytotoxicity of agents. This result shows that eye irritation potency by these agents were not correlated with skin irritation potency, and cytotoxicity in HaCaT cells.

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The Inhibitory Effects of Poncirin against Nickel Induced Cytotoxicity (Poncirin의 니켈에 대한 세포독성억제효과)

  • Yang, Seung-Jin;Kwak, Dong-Keun;Han, Du-Suk
    • The Korea Journal of Herbology
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    • v.21 no.2
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    • pp.121-127
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    • 2006
  • Objectives : Nickel is a major metal used in the nickel-chromium alloys of most orthodontic appliances, partial denture and implants. This study was carried out for the examination of the cytotoxicity on nickel sulfide in cultured NIH3T3 fibroblasts, and poncirin effect on nickel-induced cytotoxicity. Methods : Cell viability for the MTT assay and cell adhesion activity for the XTT assay. Results : The $IC_{50}$ of nickel sulfide by the MTT assay was $93.7\;{\mu}M$. Poncirin was significantly increased the cell viability and cell adhesion activity. Conclusion : Nickel was highly toxic and poncirin has the inhibitory effects against nickel induced cytotoxicity.

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Cytotoxicity of Petroleum Ether Extract of Euonymus alatus (귀전우 Petroleum Ether 추출물의 세포독성)

  • Eun, Jae-Soon;Park, Sang-Ho;Kweon, Jin;Kim, Young-Ahn;Kang, Sung-Young;Oh, Chan-Ho;Jeon, Hoon
    • Korean Journal of Pharmacognosy
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    • v.26 no.3
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    • pp.253-258
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    • 1995
  • The purpose of this research was to investigate the effects of petroleum ether extract of Euonymus alatus (EAP) on the proliferation of human tumor cells. EAP inhibited the proliferation of HeLa, Hep G2, KHOS/NP and A431 cells. The cytotoxicity of doxorubicin on human tumor cells and Balb/c 3T3 cells were increased by the combination of EAP. EAP did not affect the proliferation of Balb/c 3T3 cells, mouse spleen cells and human lymphocytes. These results suggest that EAP has the cytotoxicity on human tumor cells without cytotoxicity on Balb/c 3T3 cells, mouse spleen cells and human lymphocytes, and increase the cytotoxicity of doxorubicin.

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Cytotoxicity and Antioxidative Activity of Artemisiae Argi Folium Alcoholic Extracts and Their Fractions (애엽(艾葉) 에탄올 및 메탄올 추출물과 용매별 분획의 세포독성과 항산화활성)

  • Lee, Kyoung-In;Cho, Joo-Hyun;Pyo, Byoung-Sik
    • Korean Journal of Medicinal Crop Science
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    • v.19 no.6
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    • pp.421-425
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    • 2011
  • This experiment was carried out to obtain the cytotoxicity and antioxidative activity of Artemisiae Argi Folium. The total polyphenol contents in the ethyl acetate fraction of the ethanol extract and the methanol extract were 430.27mg/g and 427.84mg/g, respectively. In DPPH radical scavenging ability, $SC_{50}$ values of the ethyl acetate fraction of the ethanol extract and the methanol extract were 32.64 ${\mu}g/m{\ell}$ and 27.70 ${\mu}g/m{\ell}$ as the same level of statistical with ascorbic acid. In the cytotoxicity measurement by MTT assay, the chloroform and hexane fraction, and each extract were exhibited higher cytotoxicity than the other fractions. In particular, the ethyl acetate fractions appeared high activity in DPPH radical scavenging ability were began to show cytotoxicity in 125 ${\mu}g/m{\ell}$. As a result, the ethyl acetate fraction of Artemisiae Argi Folium extract was the most highly active fraction in antioxidative activity. However, for the use of extracts and fractions from Artemisiae Argi Folium to related fields, the setting of appropriate concentration is required.