• 제목/요약/키워드: DAT

검색결과 425건 처리시간 0.025초

Preparation of Dopamine Transporter-specific Antibodies Using Molecular Cloned Genes

  • Lee, Shee-Yong;Im, Suhn-Young;Kim, Kyeong-Man
    • Archives of Pharmacal Research
    • /
    • 제22권3호
    • /
    • pp.262-266
    • /
    • 1999
  • Dopamine transporter (DAT) plays the most important role in terminating the actions of dopamines released into the synaptic cleft. DAT is also the target of various psychotropic drugs such as cocaine and amphetamine. In this study were prepared DAT-specific antibodies using the 2nd extracellular loop of rat DAT as an antigen. The 2nd extracellular loop of the rat DAT was expressed in bacterial as a fusion protein with glutathione-S-transferase, and injected ito rabbits to raise antibodies. Produced antibodies clearly recognized the rat DAT in ELISA, immunoblotting, and immumoprecipitation. As expected from the high sequence homology between the rat and human DAT, the antibodies raised for the rat DAT cross-reacted with the human DAT in the immunoblotting. Considering the specificity for DAT with wide range of applications such as ELISA, immunoblotting, and immunoprecipitation, these antibodies would be valuable tool for understanding the pharmacological actions of dopamine transporter and drug addition.

  • PDF

Criteria of Nitrate Concentration in Soil Solution and Leaf Petiole Juice for Fertigation of Cucumber under Greenhouse Cultivation in Gyeonggi region

  • Park, Jung-Soo;Roh, Ahn-Sung;Jang, Jae-Eun;Kang, Chang-Sung;Kim, Hee-Dong
    • 한국토양비료학회지
    • /
    • 제48권4호
    • /
    • pp.295-304
    • /
    • 2015
  • To develop a technique for efficient management of fertility for cucumber in greenhouse, a quick test method to quantify nitrate ($NO_3{^-}$) content in soil solution and leaf petiole juice using a simple instrument that are easy to use for farmers was investigated. N fertilizer (urea) was applied at 0, 50, 100 and 200% levels of the recommended application rate from 30 days after transplanting to harvest by soil fertigation treatments. Stable results were obtained from analysis of nitrate ($NO_3{^-}$) using top $10^{th}$ or $11^{th}$ leaf petioles collected between 10 to 11 am in the morning. Under the semiforcing culture, $NO_3{^-}$ content of leaf petiole juice was highest at 60 days after transplanting (DAT) at all fertigation treatments. Appropriate $NO_3{^-}$content of leaf petiole juice was $2,418{\pm}78{\sim}2,668{\pm}118$ at 45 DAT, $3,032{\pm}90{\sim}3,332{\pm}63$ at 60 DAT, $2,709{\pm}50{\sim}3,158{\pm}155$ at 75 DAT, $2,535{\pm}49{\sim}2,907{\pm}83$ at 90 DAT, and $2,242{\pm}48mg\;L^{-1}$ at 105 DAT. In addition, appropriate $NO_3{^-}$ content of soil solution was $167{\pm}9{\sim}212{\pm}15$ at 45 DAT, $83{\pm}10{\sim}112{\pm}12$ at 60 DAT, $49{\pm}3{\sim}92{\pm}6$ at 75 DAT, $71{\pm}9{\sim}103{\pm}9$ at 90 DAT, and $73{\pm}9mg\;L^{-1}$ at 105 DAT. The cucumber yield at 100% N level of fertigation was $7,770kg\;10a^{-1}$ and no difference in yield was found at 200% N level of fertigation. However, there was 12% decrease in yield at 50% N fertigation and, 17% decrease at 0% N fertigation. Under retarding culture, $NO_3{^-}$ concentration of leaf petiole juice was highest at 55 days after transplanting (DAT) at all fertigation treatments. Appropriate $NO_3{^-}$ content of leaf petiole juice was $2,464{\pm}102{\sim}2,651{\pm}33$ at 45 DAT, $3,025{\pm}71{\sim}3,314{\pm}84$ at 55 DAT and $2,488{\pm}92mg\;L^{-1}$ at 65 DAT, respectively. Appropriate $NO_3{^-}$ content of soil solution was $111{\pm}10{\sim}155{\pm}14$ at 45 DAT, $93{\pm}7{\sim}147{\pm}14$ at 55 DAT, $67{\pm}4mg\;L^{-1}$at 65 DAT, respectively. The cucumber yield at 50% N fertigation was not different from $1,697kg\;10a^{-1}$ of 100% N fertigation level and even with that of the 200% N fertigation. However, there was 21% decrease in yield at 0% N fertigation.

재식밀도를 달리한 한국 잔디의 생장반응 (Growth Response of Korean Lawngrass (Zoysia Japonica Steud.) Imposed by Different Plant Densities)

  • 심재성
    • 아시안잔디학회지
    • /
    • 제3권1호
    • /
    • pp.5-12
    • /
    • 1989
  • Tiller numbers per Unit area were increased as plant density heightened followed by little difference in tiller number in 110 days after transplanting(ll0DAT), this being caused by the immediate increment of tiller since' 90DAT During the growth period, the greatest number of green leaves per unit area was attained in 90DAT of both 160-density and 200-density plots, and in l00DAT of 40-, 80-, and I 20-density plots. Thus, the period to reach the maximum green leaf number with the high plant density was earlier than with the' low plant density. The increment of plant density increased the DM weight of ahoveground part and, with 40-densitytreatment. DNI weight was increased 6.1 times as much up to 110DAT as up to 7ODAT but 2 to 2.9 times with other ehe'nsity treatments. The' rate of increase in stolon length was greatest at the period between 80 and 90DAT when DM weight of stolon showed a large increment. Meanwhile, the average number of stolon was 1.7 at 70DAT but was increased up to 10 at 110DAT probably due to accelerative appeance of Ist stolon branch since 8.0DAT. DM weight of stolon was found to exceeded that of root after 90DAT Thus it was concluded that, when turfs is estahlished from sprig. it may be desirable for first mowing practice to be made at least within l00 days after planting.

  • PDF

건설 사업관리 DAT 활용분야 및 적용방법 분석 (Effective Areas for DAT Utilization in Construction Projects)

  • 서큰솔;박종순;정영수
    • 한국건설관리학회논문집
    • /
    • 제11권2호
    • /
    • pp.15-24
    • /
    • 2010
  • 건설 사업관리의 자료 수집 및 분석에 있어 아직도 많은 부분이 현장관리자의 수작업에 의존하고 있다. 이런 수작업에 의한 관리 방법의 대표적인 문제점으로서는, 자세한 현장자료의 획득이 어려울 뿐만 아니라, 가능하더라도 이를 위해서는 상당한 비용과 노력이 소요된다. 따라서 효율적인 사업관리를 위한 첨단 DAT(Data Acquisition Technology) 기술이 적용을 통한 자료수집 자동화가 시도되고 있으나, 대부분 기존 연구가 단일 공종이나 혹은 일부 주요 관리만을 대상으로 한 DAT 적용 연구로 이루어져 프로젝트 전체관점에서 분석한 종합적 연구는 전무한 상태이다. 따라서 본 연구는 DAT를 건설 사업관리와 관련하여 포괄적으로 분석함으로써 활용분야를 살펴보고 대체 활용 방향을 제안한다.

모바일 게임 개발에서 4-DAT를 이용한 개발 프로세스 알고리즘 (Development Process Algorithm using 4-DAT on Mobile Game Development)

  • 이지원;박성준
    • 한국콘텐츠학회:학술대회논문집
    • /
    • 한국콘텐츠학회 2010년도 춘계 종합학술대회 논문집
    • /
    • pp.76-78
    • /
    • 2010
  • 본 논문에서는 소프트웨어 개발 프로세스 분석 도구인 4-DAT를 이용하여 XP와 스크럼을 비교분석하여 모바일 게임 개발에 최적화된 개발 방법을 제안한다. 새로운 개발 방법은 기존의 4-DAT가 포함하고 있는 4차원의 고려사항을 3차원으로 재정의하여 통합한 것으로 모바일 환경에서 기존의 XP와 스크럼이 가지는 제약성을 보완할 수 있다. 실험의 방법으로서 4-DAT가 가지는 변수를 적용하여 기초 자료를 도출하였고, 재구성한 3-DAT를 기반으로 상황 변수를 적용하여 분석하였다.

  • PDF

도파민 수송체의 기능적 특성 및 발현에 관한 연구 (Functional Characterization and Regional Expression of Dopamine Transporter)

  • 이상훈;이송득;성기욱;이동섭;이용성;고재경
    • 약학회지
    • /
    • 제39권2호
    • /
    • pp.161-168
    • /
    • 1995
  • Brain dopamine systems play a central role in the control of movement, hormone release, and many complex behavior. The action of dopamine at its synapse is terminated predominately by high affinity reuptake into presynaptic terminals by dopamine transporter (DAT). The dopamine transporter(DAT) is membrane protein localized to dopamine-containing nerve terminals and closely related with cocaine abuse, Parkinsonism, and schizophrenia. In present study, the recombinant plasmid pRc/CMV-DAT, constructed by subcloning of a cDNA encoding a bovine DAT into eukaryotic expression vector pRc/CMV, was stably transfected into CV-1 cells(monkey kidney cell line). The DAT activities in the cell lines selected by Geneticin$^{R}$ were determined by measuring the uptake of $[^3H]$-dopamine. The transfected cell lines showed 30-50 fold higher activities than untransfected CV-1 cell line, and this result implies that DAT is well expressed and localized in transfected cells. The transfected cells accumulated $[^3H]$-dopamine in a dose-dependent manner with a $K_{m}$ of 991.6nM. Even though high doses of norepinephrine, epinephrine, serotonin, and choline neurotransmitters inhibited the uptake of $[^3H]$-dopamine, DAT in transfected cell line was proven to be much more specific to dopamine. The psychotropic drugs such as GBR12909, CFT, normifensine, clomipramine, desipramine, and imipramine inhibited significantly the dopamine uptake in tissue culture cells stably transfected with DAT cDNA. Radioactive in situ hybridization was done to map the cellular localization of DAT mRNA-containing cells in the adult rat central nervous system. The strong hybridization signals were detected only in the substantia nigra pars compacta and ventral tegmental area. The restricted anatomical localization of DAT mRNA-containing cells confirms the DAT as a presynaptic marker of dopamine-containing cells in the rat brain.

  • PDF

주의력결핍 과잉운동장애에서 도파민 수송체 유전자 다형성 (Dopamine Transporter Gene Polymorphism in ADHD)

  • 신동원
    • 생물정신의학
    • /
    • 제9권1호
    • /
    • pp.8-14
    • /
    • 2002
  • Patients with attention deficit hyperactivity disorder(ADHD) have symptoms of inattention, hyperactivity, impulsivity. Symptoms of ADHD are responsive to medications such as methylphenidate, dextroamphetamine, pemoline, and bupropion. The functional change of the dopamine transporter is related to the therapeutic effect of these drugs. This can be one reason for the dopamine transporter to be emphasized in the research field of ADHD. ADHD has a genetic tendency. Since dopamine transporter gene(DAT1) knock out mice were reported to be several times more active than normal mice in a novel situation, lights has been shed on DAT1 as a candidate gene for ADHD. Though there have been several studies which reported an association between DAT1 and ADHD, the association between DAT1 and ADHD is not conclusive. Since Vandenbergh reported the DAT1 polymorphism with variable number of tandem repeats(VNTR), and the racial differences in allelic frequencies of the DAT1, wide ethnic variation in the distribution of the DAT1 polymorphism had been confirmed. Wide ethnic variation in the distribution of the DAT1 suggested that there might be ethnic difference in the association between DAT1 and ADHD. Before applying previous findings to Koreans, verification might be needed for Korean patients with ADHD.

  • PDF

코카인 결합과 관련된 도파민 수송체의 아미노산 구조 (Amino Acid Structure of Dopamine Transporter Responsible for Cocaine Binding)

  • 장미윤;전대준;오동렬;이용성;이상훈
    • 약학회지
    • /
    • 제43권6호
    • /
    • pp.743-750
    • /
    • 1999
  • Human and bovine dopamine transporters (DAT) demonstrate discrete functional differences in the dopamine (DA) transport and cocaine binding. The functional analyses on the chimeras of human and bovine DAT have revealed that the region from the $133^{rd}{\;}to{\;}186^{th}$ residue(encompassing the $3^{rd}$ trans-membrane domain (TM) is responsible for the substrate transport and cocaine binding. The present studies have been done to find out the specific amino acid(s) which is essential for the binding of cocaine to DAT by interchanging the amino acids in that region between human and bovine DAT. When isoleucine, the $152^{nd}$ residue of chimera B3 (bovine DAT sequence) was transformed back to valine, the human DAT residue at the identical position, the cocaine binding was remarkably recovered to 98% of the human DAT values. In addition, the cocaine binding of the human DAT was decreased by 57% by substituting isoleucine for valine at position 152. When isoleucine at position 152 of the chimera B3 was converted to the other amino acids to provide an possible molecular basis for the functional role of the $152^{nd}$ residue, only the conversion to alanine among acids tested significantly the cocaine by 34%, but these effect were not as much as those by the conversion to valine. In conclusion, valine at position 152 is a crucial amino acid for the interaction of cocaine to the DAT.

  • PDF

3-DAT 기반의 모바일 게임 개발 프로세스 평가 방법 (3-DAT Based Evaluation Method for Mobile Game Development Process)

  • 이지원;김경식;박성준
    • 한국게임학회 논문지
    • /
    • 제10권6호
    • /
    • pp.15-23
    • /
    • 2010
  • 4-DAT는 일반적인 소프트웨어 개발 방법인 XP와 Scrum을 분석하는 도구이다. 본 논문에서는 3-DAT를 기반으로 모바일 게임 개발 환경에 맞는 새로운 팽가 방법을 제안한다. 4-DAT 평가 방법은 모바일 게임 개발에 있어서 평가 항목 및 불필요한 요소가 있기 때문에 3차원으로 최적화된 명가 방법을 적용하였다. 본 논문에서는 새롭게 정의한 3-DAT 기반의 팽가 방법을 위해 P. Abrahamsson, Boehm. B, R. Tumer의 이론을 기초로 하여 5항목, 20세부 기준의 개발 상황 연수를 정의하였다. 실험 방법은 4개월 동안 진행된 5개의 모바일 게임 개발 데이터를 기반으로 개발 상황 연수와 재구성된 3-DAT에 적용하였고, 구현한 시뮬레이터를 통해 3차원 그래프를 도출하였다. 실험 내용은 개발팀의 의존성, 편중성, 유연성에 대한 요소를 분석하였다. 실험 결과로 단기간의 모바일 게임 개발 시 의존성이 높고, 편중성이 낮으며, 유연성이 높을 때 위험성이 적은 개발을 완료 할 수 있음을 알 수 있었다.

진도관리 자동화를 위한 자료수집기술(DAT) 선정 방법 (Data Acquisition Technology (DAT) Selection Algorithm for Automated Progress Measurement and Management)

  • 강승희;정영수
    • 한국건설관리학회논문집
    • /
    • 제13권1호
    • /
    • pp.77-86
    • /
    • 2012
  • 건설 프로젝트의 성과 측정 및 관리에 있어 진도관리는 기본적이며 핵심적인 업무기능이다. 오랜 기간 동안 다양한 진도 관리 기법이 연구 개발되고 있음에도 불구하고, 자료수집을 위한 과도한 업무부담은 체계화된 진도관리의 장애요소로 지적되어 왔다. 최근 자료수집기술(Data Acquisition Technology, DAT)의 발전은 자동화된 진도관리의 기회를 제공하고 하고 있다. 그럼에도 불구하고 지금까지의 DAT 연구는 특정 공종 또는 특정 기술에 집중하고 있어, 건설사업 전체의 진도관리 자동화에 대한 연구는 이루어지지 않았다. 이러한 관점에서, 본 연구는 건설사업 공종별 최적의 DAT를 선정하는 방법론을 제안하였다. DAT 선정방법은 각 공종별 진도측정의 방법, 대상, 범위, 기술 등의 유형을 분류하여, 이를 DAT 특성별로 최적치를 도출하는 알고리즘으로 구성된다. 제안된 방법론의 검증을 위하여 사례 프로젝트를 이용한 기계적 분석을 실시하고 또한 실무자 평가를 수행한 결과, 대부분 공종에서 동일한 결과를 얻었다. 또한 사례현장 평가결과 중의 하나로서, 노무자의 층별 출입정보를 RFID를 활용하여 측정하는 것이 가장 효율적이며 가장 많은 공종에 적용이 가능한 것으로 나타났다.