• Title/Summary/Keyword: DTA

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Mechanism and Activation Parameters $({\Delta}H^{\neq},\;{\Delta}S^{\neq}$ and ${\Delta}V^{\neq})$ of Electron Transfer Reaction Between $Co^{II}CyDTA\;and\;Fe^{III}$CN Complex Ions (Co(II)-CyDTA와 Fe(III)-CN 착이온간의 전자이동반응에서 활성화파라미터 $({\Delta}H^{\neq},\;{\Delta}S^{\neq}$${\Delta}V^{\neq})$ 와 반응메카니즘)

  • Yu Chul Park;Seong Su Kim
    • Journal of the Korean Chemical Society
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    • v.33 no.3
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    • pp.273-280
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    • 1989
  • The spectra of the $Co^{II}CyDTA$(CyDTA: cyclohexyldiaminetetraacetic acid) complex have been measured in aqueous solution of pH = 6-13.2. The red shift of the spectrum in the more basic solution was ascribed to the transformation of $CoCyDTA^{2-}$ into $CoCyDTA(OH)^{3-}$. The equilibrium constant, $K_{OH} = [CoCyDTA(OH)^{3-}]/[CoCyDTA^{2-}][OH^-]$ was $75M^{-1}$ at $40^{\circ}C$. The electron transfer reactions of $CoCyDTA^{2-}$ and $CoCyDTA(OH)^{3-}$ with $Fe(CN)_6^{3-}$ have been studied using spectrophotometric technique in the range of pH applied to the determination of equilibrium constant. The pseudo first-order rate constants observed ($k_{obs}$) were not changed upto pH = 10.8, but increased with increasing pH in the range of pH = $10.8{\sim}13.0$. The rate law reduced in the range of pH = 6-13 was $k_{obs} = (k_3[CoCyDTA^{2-}] + k_4[CoCyDTA(OH)^{3-}])/(1+K_1[CoCyDTA^{2-}])$. The rate constants of the reactions (3a) and (3b), $k_3$ and $k_4$ respectively have been determined to be 0.529 and $4.500M^{-1}sec^{-1}$ at $40^{\circ}C$. The activation entropies (147{\pm}1.1JK^{-1} mol^{-1}$ at pH = 10.8) and activation volumes $(6.25cm^3mol^{-1}, pH = 10.8)$ increased with increasing pH, while the activation enthalpy (12.44 ${\pm}$ 0.20 kcal/mole) was independent of pH. Using the pH effect on the rate constants, the activation entropies and the activation volumes, the mechanism of the electron transfer reaction for $Co^{II}-Fe^{III}$ system was discussed.

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Preparation of Diphtheria Toxin A Chain from Escherichia coli

  • Lee, Jong-Soo;Yoon, Kyoung-Bum;Park, Jong-Won;Choi, Suk-Jung
    • BMB Reports
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    • v.30 no.2
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    • pp.144-149
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    • 1997
  • An expression vector was constructed containing the gene encoding diphtheria toxin A (DTA) which was placed after a T7 promoter. Cytoplasmic expression of the DTA gene resulted in the formation of an insoluble inclusion body. The inclusion body was collected after the complete lysis of the cell, and subsequent washing with 0.1% Triton X-100 released 16~30% of DTA protein from the inclusion body along with other contaminating proteins. The released DTA protein was purified by dialysis. The remaining pellet was dissolved in 8 M urea containing 5% ${\beta}-mercaptoethanol$, and the denatured DTA was renatured by the dilution-dialysis method. The total yield was 35%, and about 5 mg DTA was obtained from 1 L culture. The DTA protein has a free sulfhydryl group exposed to the protein surface, and was shown to have a tendency to dimerize through disulfide formation in the absence of ${\beta}-mercaptoethanol$. The utility of the sulfhydryl group was tested for the construction of recombinant toxins.

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Development of a Quadrivalent Combined DTaP-HepB Vaccine with a Low Toxicity and a Stable HBsAg Immunogenicity

  • Bae, Cheon-Soon;Park, Kwung-Nam;Ahn, Sang-Jeom;Kim, Jong-Su;Hur, Byung-Ki
    • Journal of Microbiology and Biotechnology
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    • v.12 no.5
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    • pp.787-792
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    • 2002
  • When developing a combined DTaP-HepB vaccine, toxicity and HBsAg immunogenicity are both important considerations. Thus, for a combined DTaP-HepB vaccine with a low toxicity, the effect of the DTaP content and $Al(OH)_3$, gel concentration on the vaccine toxicity was investigated. Within the range studied, the higher the concentrations, the higher the vaccine toxicity. The importance of the tetanus toxoid content in the combined DTaP-HepB vaccine was also revealed. A higher concentration of the tetanus toxoid was found to have a negative effect on the stability of the HBsAg immunogenicity in the combined vaccine. Accordingly, considering the factors affecting toxicity and HBsAg immunogenicity, a novel DTaP-HepB vaccine (30 Lf/ml of diphtheria toxoid, 5 Lf/ml of tetanus toxoid, 10 $\mu\textrm{g}$ PN/ml of acellular pertussis, 24 $\mu\textrm{g}$/ml of HBsAg, and 500 $\mu\textrm{g}$ Al/ml of $Al(OH)_3$ gel) was developed. It has a low toxicity and a stable HBsAg immunogenicity and also satisfies the potency criteria of K-FDA for a combined DTaP vaccine.

A Comparative Study on Exciting Force for Luminaire of Naval Vessels According to Sailing Condition (함정 운항에 따른 조명등기구 가진력 비교 연구)

  • Park, Mi-You;Cho, Heung-Gi;Lee, Kowan-Woo;Yu, Young-Soo;Cho, Kyu-Lyong
    • Journal of the Korea Institute of Military Science and Technology
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    • v.15 no.4
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    • pp.390-397
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    • 2012
  • According to sailing condition, an exciting force for luminairie of naval vessels was measured and analyzed. A naval combat service support boat and P${\bigcirc}{\bigcirc}$(patrol killer guided missile) were selected as a target naval vessel. Due to their small size and fast speed, they received excessive vibration than other bigger naval vessels like destroyer. The vibration of luminairie was measured on their cruising and maximum speed. This could analyze exciting force for luminairie of naval vessels. Using this analyzed data, the validity of military standard for naval vessel was verified.

A Study on Improvement Plan for LST-II LCM Cradle Damage (LST-II급 함정 함수 LCM 거치대 손상에 대한 개선방안 연구)

  • Choi, Sang-Min;Beak, Yong Kawn;Jung, Young In;Hwang, In Ha;Baek, Jae Sung
    • Journal of Korean Society for Quality Management
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    • v.47 no.1
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    • pp.139-150
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    • 2019
  • Purpose: LST-II is a special naval ship to carry out landing operations by transporting tanks, armored vehicles and military vehicles. Bad weather, maximum wave height of 4-5 m, caused damage to the LCM cradles while the LST-II No.O ship was moving to Thailand for training in February 2016. Methods: Based on the results of the field check, DTaQ conducted a study on the causes analysis and improvement measures. Results: The improvement plan that was derived was verified through a structural analysis and the improvement plan was applied to the follow-up ships. Conclusion: The improvement of LCM cradle has increased the safety of the crew and landing forces, and improved the operational efficiency.

A New Quantification Method for Multi-Unit Probabilistic Safety Assessment (다수기 PSA 수행을 위한 새로운 정량화 방법)

  • Park, Seong Kyu;Jung, Woo Sik
    • Journal of the Korean Society of Safety
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    • v.35 no.1
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    • pp.97-106
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    • 2020
  • The objective of this paper is to suggest a new quantification method for multi-unit probabilistic safety assessment (PSA) that removes the overestimation error caused by the existing delete-term approximation (DTA) based quantification method. So far, for the actual plant PSA model quantification, a fault tree with negates have been solved by the DTA method. It is well known that the DTA method induces overestimated core damage frequency (CDF) of nuclear power plant (NPP). If a PSA fault tree has negates and non-rare events, the overestimation in CDF drastically increases. Since multi-unit seismic PSA model has plant level negates and many non-rare events in the fault tree, it should be very carefully quantified in order to avoid CDF overestimation. Multi-unit PSA fault tree has normal gates and negates that represent each NPP status. The NPP status means core damage or non-core damage state of individual NPPs. The non-core damage state of a NPP is modeled in the fault tree by using a negate (a NOT gate). Authors reviewed and compared (1) quantification methods that generate exact or approximate Boolean solutions from a fault tree, (2) DTA method generating approximate Boolean solution by solving negates in a fault tree, and (3) probability calculation methods from the Boolean solutions generated by exact quantification methods or DTA method. Based on the review and comparison, a new intersection removal by probability (IRBP) method is suggested in this study for the multi-unit PSA. If the IRBP method is adopted, multi-unit PSA fault tree can be quantified without the overestimation error that is caused by the direct application of DTA method. That is, the extremely overestimated CDF can be avoided and accurate CDF can be calculated by using the IRBP method. The accuracy of the IRBP method was validated by simple multi-unit PSA models. The necessity of the IRBP method was demonstrated by the actual plant multi-unit seismic PSA models.

A Study on The Mass Production Weapon System Parts Localization System Engineering Development Management Process Application based on ISO/IEC/IEEE 15288 (ISO/IEC/IEEE 15288 기반 양산단계 무기체계 부품국산화 체계공학 개발관리 절차 적용 연구)

  • Kim, Jang-Eun;Shim, Bo-Hyun;Cho, Yu-Seup;Sung, In-Chul;Han, Dong-Seog
    • Journal of Korean Society for Quality Management
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    • v.44 no.3
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    • pp.541-552
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    • 2016
  • Purpose: In this study, we propose that how to approach a effective system engineering and optimize system engineering management process for the mass production weapon system parts localization development process and success in DTaQ. Methods: To approach a effective system engineering for the mass production weapon system parts localization, we analyze a weapon system acquisition process and system engineering process of Republic of Korea and DTaQ parts localization business regulations in advance. after results of analysis of them, we implement a optimized parts localization development system engineering based on ISO/IEC/IEEE 15288. Results: In order to apply International Standard ISO/IEC/IEEE 15288 to the mass production weapon system parts localization development process, we compare the mass production weapon system parts localization acquisition environment with ISO/IEC/IEEE 15288 and analyze them. therefore, It is possible to implement a part of concept stage and development stage of ISO/IEC/IEEE total life cycle stage for the mass production weapon system parts localization development process. To achieve the technical review milestones of DTaQ parts localization business regulations in the selected stages of ISO/IEC/IEEE, the development and management agency perform 2 high rank process and 19 low rank process specified in ISO/IEC/IEEE. Conclusion: When the development and management agency perform the mass production weapon system parts localization development using the proposed system engineering approach, they should easily meet milestone through the clarified requirement and simplified System Engineering output documents in limited development period.

Determination of the Nucleation Rate Curve for Lead Titanate in the PbO-TiO$_2$-B$_2$O$_3$-BaO by Diffferential Thermal Analysis (PbO-TiO$_2$-B$_2$O$_3$-BaO 계 유리에서 PbTiO$_3$ 결정의 핵생성 곡선 결정을 위한 열시차분석법의 응용)

  • 이선우;심광보;오근호
    • Journal of the Korean Ceramic Society
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    • v.35 no.6
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    • pp.640-646
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    • 1998
  • Nucleation and crystallzation of a quaternary glass system for lead titanate glass-ceramics were in-vestigated using DTA(differential thermal analysis ) with variation of nucleation temperature and crystal growth time. Glass samples containing 60mol% of PbO-{{{{ { TiO}_{2 } }} were prepared from melts by the conventional normal cooling method in a cylindrical brass mould. The glass sample was nucleated between 40$0^{\circ}C$ and 50$0^{\circ}C$ for a given time and showed the maximum nucleation rate at 46$0^{\circ}C$ The DTA crystallization peak temperature decreased with increasing nucleating time and decreasing heating rate during DTA runs which indicated an increase of the number of nuclei produced in the system.

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The immunogenicity and safety of three-component DTaP vaccine in Korean infants (우리나라 영아에서 3가 정제 백일해 항원 DTaP 백신의 면역원성 및 안전성)

  • Kang, Jin Han;Kim, Jong Hyun;Lee, Jung Hyun;Lee, Soo Young;Hong, Young Jin;Kim, Chang Hwi
    • Clinical and Experimental Pediatrics
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    • v.50 no.4
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    • pp.355-362
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    • 2007
  • Purpose : We conducted the study to evaluate the immunogenicity and safety of three component DTaP vaccine ($Infanrix^{(R)}$) in a group of Korean healthy infants on a three-dose primary vaccination. And we compared the immunogenicity of this DTaP vaccine with two component DTaP vaccine which has been widely used in Korea. Methods : We enrolled one hundred fifty one healthy infants aged 8-9 weeks. These infants were vaccinated at age 2, 4 and 6 months of age with three component DTaP vaccine. Solicited adverse events were actively monitored for 72 hours following each vaccination, and all adverse events after each vaccination were observed for three weeks. Anti-diphtheria toxoid Ab., anti-tetanus toxoid Ab., anti-pertussis toxin Ab., anti-filamentous hemagglutinin Ab., and anti-pertactin Ab. were measured using ELISA for assessing immunogenicity of study vaccine in 60 infants. Immunogenicity analysis of two component DTaP vaccine was performed with same methods in 14 infants as control. Results : The seroconversion rates of anti-diphtheria toxoid Ab, anti-tetanus toxoid Ab. anti-filamentous hemagglutinin Ab. were 100% in both group. Seroconversion rate of anti-pertactin Ab in study group was 100%, but the rate in control group was 50%. However, geometric mean concentration of anti-pertussis toxin Ab. was higher in control group. Mild local and systemic reactions were observed within three days after vaccination, and no serious adverse events related study vaccine were happened during study period. Conclusion : Our study results suggest that three component DTaP vaccine ($Infanrix^{(R)}$) is a well-tolerable and high immunogenic vaccine, especially anti-Pertactin Ab. of the study vaccine is very immunogenic. It can be available as routine DTaP vaccination in our infants.