• Title/Summary/Keyword: D_1%24

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Transmucosal Delivery of Luteinizing Hormone-Releasing Hormone(LHRH): Enzymatic Proteolysis of $[D-Ala^6]$ LHRH and Inhibitory Effect of Medium Chain Fatty Acid Salts in Rabbit Mucosa (황체호르몬 유리호르몬(LHRH)의 경점막 수송: 토끼 점막균질액 중에서 $[D-Ala^6]$ LHRH의 효소적 분해 특성 및 중쇄지방산염의 안정화 효과)

  • Park, Jeong-Sook;Chung, Youn-Bok;Han, Kun
    • YAKHAK HOEJI
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    • v.38 no.2
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    • pp.202-210
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    • 1994
  • To investigate the feasibility of mucosal delivery of $[D-Ala^6]$ LHRH, a potent analogue of LHRH, enzymatic proteolysis of $[D-Ala^6]$ LHRH and inhibitory effect of medium chain fatty acid salts(MFA) were studied using rabbit mucosal homogenate. $[D-Ala^6]$ LHRH incubated in homogenates of rectal(RE), nasal(NA) and vaginal(VA) mucosa were assayed by HPLC. The degradation of $[D-Ala^6]$ LHRH followed the first order kinetics. The degradation products were found as $[D-Ala^6]$ $LHRH^{1-7}$(m-i), to a lesser extent, $[D-Ala^6]$ $LHRH^{1-9}$(m-ii) and $[D-Ala^6]$ $LHRH^{1-3}$(m-iii) by the method of amino acid analysis(PITC method). The formation of$[D-Ala^6]$ $LHRH^{1-7}$ was not inhibited by the addition of disodium ethylenediaminetetraacetic acid but inhibited by sodium tauro-24,25-dihydrofusidate, suggesting that endopeptidase 24.11(EP 24.11) cleaves the $Leu^7-Arg^8$ bond of $[D-Ala^6]$ LHRH and is the primary $[D-Ala^6]$ LHRH degrading enzyme. The patterns of $[D-Ala^6]$ LHRH degradation indicated that EP 24.11 exists in each mucosal homogenate with the order of RE>NA>VA. MFA significantly inhibited the proteolysis of $[D-Ala^6]$ LHRH. The addition of sodium caprate(1.0%) or sodium laurate(0.5%) to the each mucosal homogenate completely protected $[D-Ala^6]$ LHRH from the degradation.

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Changes in plasma lipoxin A4, resolvins and CD59 levels after ischemic and traumatic brain injuries in rats

  • Jung, Jun-Sub;Kho, A Ra;Lee, Song Hee;Choi, Bo Young;Kang, Shin-Hae;Koh, Jae-Young;Suh, Sang Won;Song, Dong-Keun
    • The Korean Journal of Physiology and Pharmacology
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    • v.24 no.2
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    • pp.165-171
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    • 2020
  • Ischemic and traumatic brain injuries are the major acute central nervous system disorders that need to be adequately diagnosed and treated. To find biomarkers for these acute brain injuries, plasma levels of some specialized pro-resolving mediators (SPMs, i.e., lipoxin A4 [LXA4], resolvin [Rv] E1, RvE2, RvD1 and RvD2), CD59 and interleukin (IL)-6 were measured at 0, 6, 24, 72, and 168 h after global cerebral ischemic (GCI) and traumatic brain injuries (TBI) in rats. Plasma LXA4 levels tended to increase at 24 and 72 h after GCI. Plasma RvE1, RvE2, RvD1, and RvD2 levels showed a biphasic response to GCI; a significant decrease at 6 h with a return to the levels of the sham group at 24 h, and again a decrease at 72 h. Plasma CD59 levels increased at 6 and 24 h post-GCI, and returned to basal levels at 72 h post-GCI. For TBI, plasma LXA4 levels tended to decrease, while RvE1, RvE2, RvD1, and RvD2 showed barely significant changes. Plasma IL-6 levels were significantly increased after GCI and TBI, but with different time courses. These results show that plasma LXA4, RvE1, RvE2, RvD1, RvD2, and CD59 levels display differential responses to GCI and TBI, and need to be evaluated for their usefulness as biomarkers.

Syntheses and realization of Quaternary Galois Field Sum-Of-Product(QGFSOP) expressed 1-variable functions Permutational Literals (치환리터럴에 의한 Quaternary Galois Field Sum-Of-Product(QGFSOP)형 1-변수 함수의 합성과 실현)

  • Park, Dong-Young;Kim, Baek-Ki;Seong, Hyeun-Kyeong
    • Journal of Advanced Navigation Technology
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    • v.14 no.5
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    • pp.710-717
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    • 2010
  • Even though there are 256 possible 1-qudit(1-variable quantum digit) functions in quaternary logic, the most useful functions are 4!=24 ones capable of representing in QGFSOP expressions by possible permuting of 0,1,2, and 3. In this paper, we propose a permutational literal(PL) representation and a QPL(Quaternary PL) gate which use the operands of a multiplicand A and an augend D in $Ax^C$+D(GF4) operation as a control variable of multi-cascaded PLs. And we also present new PL synthesis algorithms to synthesize QGFSOP expressed 24 (1-qudit) functions by applying three PL operators as ab(mutual permutation), + D(addition), and XA (multiplication). Finally architectures, circuits, and a CMOS implementation to realize proposed PL synthesis algorithms for $Ax^C$+D(GF4) functions are presented.

Design of a Single-Balanced Diode Mixer at 24GHz (24GHz대역 단일 평형 다이오드 주파수 혼합기의 설계 및 제작)

  • 강상록;박창현;김장구;조현식;한석균;최병하
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2003.10a
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    • pp.66-70
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    • 2003
  • In this paper a plannar singly balanced diode Miter for 24GHz band application is designed and implemented using a microstrip line and two schottky barrier beam lead mixer diodes. The implemented mixer have a conversion loss of 6 [dB], LO/RF isolation of 23 [dB], input 1dB compression point of 4 [dBm]. this diode mixer would be useful for homedyne radar.

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The Effects of 1,25- Dihydroxyvitamin $D_3$ on Expression of IGF-I Gene and Cellular Proliferation in MC3T3-E1 Cells (골아세포의 IGF-I 유전자 발현 및 세포증식에 대한 1,25-dihydroxyvitamin $D_3$의 영향)

  • Choi, Hee-Dong;Lee, Jae-Mok;Suh, Jo-Young
    • Journal of Periodontal and Implant Science
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    • v.30 no.1
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    • pp.39-52
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    • 2000
  • Polypeptide growth factor belong to a class of potent biologic mediator which regulate cell differentiation, proliferation, migration and metabolism. 1,25-dihydroxyvitamin $D_3$ decrease cell proliferation, and stimulate alkaline phosphatase activity which express in osteoblast during cell differentiation period. IGF-I is known to stimulate cell proliferation and differentiation too. 1,25-dihydroxyvitamin $D_3$ is known to increase IGF-I binding sites and IGF binding protein which inhibite the effect of IGF. The purpose of this study is to evaluate potential role of IGF-I as mediator that control the action of 1,25-dihydroxyvitamin $D_3$. MC3T3-E1 cell were seeded $5{\times}10^5/ml$ at 100mm culture plate in ${\alpha}-MEM$ containing 10% fetal bovine serum. After 48 hour incubation period, medium were changed ${\alpha}-MEM$ containing 5% fetal bovine serum. After 24 hours, $10^{-9}M$ 1,25-dihydroxyvitamin $D_3$ added. Total mRNA was extracted at 0, 6, 24, 48, 72 hour. PRPCR method was programed for the detection of IGF-I mRNA. In the both groups of 1,25-dihydroxy vitamin $D_3$ treated and control, alternative splicing form of IGF-I, IGF-IA and IGF-IB were expressed. In the 1,25-dihydroxyvitamin $D_3$ treated group, IGF-I mRNA expression was matained until 24 hour, there after expression was decresed. MC3T3-E1 cell were seeded $2.5{\times}10^4/ml$ at 24well plate in ${\alpha}-MEM$ containing 10% fetal bovine serum. After 48 hour incubation period, medium were changed ${\alpha}-MEM$ containing 3% fetal bovine serum. After 24 hours, $10^{-9}M$ 1,25-dihydroxyvitamin $D_3$ and 10 ng/ml IGF-I were added separately or together. Cell were cultured for 1 and 3 days, $2{\mu}Ci/ml\;[^3H]$ -thymidine was added for the last 24h of culture of each days. ${[^3H]}$-thymidine incorporation in to DNA was measured and expressed counter per minute(CPM). DNA synthetic activity was significantly decreased by 1,25-dihydroxyvitamin $D_3$ both at 1 day and 3 day, and in the combination group of 1,25-dihydroxyvitamin $D_3$ and IGF-I, DNA synthetic activity was also decreased both at 1 day and 3 days. IGF-I did not affect the DNA synthetic activity compared to control group both at 1 day and 3 day. From the above results, 1,25-dihydroxyvitamin $D_3$ was potent inhibitor of cell proliferaton in MC3T3-E1 cells. It assumed that the effect of 1,25-dihydroxyvitamin $D_3$ on osteoblast proliferation may be mediated in part by decreased level of IGF-I.

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Steroid compounds from the marine sponge Raspilia hirsute

  • Rho Jung-Rae
    • Journal of the Korean Magnetic Resonance Society
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    • v.10 no.1
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    • pp.89-95
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    • 2006
  • The methanolic extract of the marine sponge Raspilia hirsute collected from Keomun Island resulted in three types of sterols: a mixture of (24S)-Poriferasta-5, 25-diene-$3\beta$, 24-diol and (24R)-Stigmasta-5, 25-diene-$3\beta$, 24 -diol (1), 25,26,27-Trinorcholest-5-en-$3\beta$,24-diol (2), and Pregn-5-en-20-on-$3\beta$-ol (3). The isolation and structural determination of these sterols are reported here. Compound 1 showed moderate cytotoxicity against human Leukemia cell line K562.

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Cholecalciferol Metabolism Metabolic Conversion and Mode of Action (Cholecalciferol 대사연구(代謝硏究)에 관(關)한 일정리(一整理))

  • Yoon, Jin-Sook
    • Journal of Nutrition and Health
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    • v.12 no.1
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    • pp.9-16
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    • 1979
  • Cholecalciferol은 피하에서 자외선과 체온의 작용을 받아 7-dehydrocholesterol로부터 Previtamin D를 거쳐 합성이 되며 이어서 간에 빨리 축적, 25 hydroxylation을 거치게 되고 신장에서 가장 활성을 띤 형태인 $1.25-(OH)_{2}CC$로 변하게 된다. 한편 신장에서는 체내의 Ca, P이 정상으로 존재하게 되면 1-hydroxylation이 억제되는 대신 24-hydroxylation이 일어나 또 다른 active form인 $24,\;25-(OH)_{2}CC$가 되는데 24-hydroxylation의 역활이 무엇인가에 대해서는 아직 구체적으로 밝혀지지 않았다. $24,\;25-(OH)_{2}CC$$1.25-(OH)_{2}CC$는 모두 공통적으로 또 다른 active form인 $1.24,\;25-(OH)_{3}CC$를 형성할 수도 있다. 그중 $1.25-(OH)_{2}CC$는 Steroid H.으로 일컬어지기도 하는 Vit. D metabolite중에서 가장 활성을 가진 형태이며 표적기관으로 크게 소장, 뼈, 신장을 들 수 있다. 소장에서의 역활은 무엇보다도 Ca흡수에 관련되는 것으로 소장에서의 Ca흡수와 Ca BP합성에 관여한다. 골격 형성과 뼈의 mineralization에 관여하는 Vit. D metabolite를의 효과에 관해서는 아직까지 일관성있는 보고가 없다. 한편 $1.25-(OH)_{2}CC$는 side chain oxidation을 거쳐 $CO_{2}$와 미지의 물질을 생성하는데 이 mechanism이 어떤 의미를 갖는지는 분명치 않다. 그밖에 또 다른 표적기관으로서 최근 타액선의 존재가 알려졌으며 Vit. D가 배설되는 경로에 대해서는 새로운 바가 없다. Vit. D가 담즙을 통해서 우선적으로 배설되고 소량이 뇨를 통해 배설되는데 metabolite들의 배설경로는 더욱 규명되어야 할 과제이다.

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Effects of Water Temperature and Photoperiod on the Oxygen Consumption Rate of Juvenile Dark-banded Rockfish, Sebastes inermis (수온과 광주기에 따른 볼락, Sebastes inermis 치어의 산소 소비율)

  • Oh Sung-Yong;Noh Choong-Hwan
    • Journal of Aquaculture
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    • v.19 no.3
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    • pp.210-215
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    • 2006
  • An experiment was conducted to investigate the effects of four water temperatures (10, 15, 20, and $25^{\circ}C$) in combination with three photoperiods (24L:0D, 12L: 12D, and OL:24D) on the oxygen consumption rate of juvenile dark-banded rockfish, Sebastes inermis (mean body weight $20.5{\pm}0.7g$). The oxygen consumption rates of S. inermis were measured in triplicate for 24 hours using a continuous flow-through respirometer. Different combinations of water temperatures and photoperiods resulted in significant differences in the mean oxygen consumption rate of S. inermis (P<0.001). The oxygen consumption increased with increasing water temperatures for all photoperiod treatments (P<0.01). Mean oxygen consumption rates at 10, 15,20 and $25^{\circ}C$ ranged $178.3\sim283.5,\;386.7\sim530.7,\;529.2\sim754.3$ and $590.0\sim785.5mg\;O_2kg^{-1}h^{-1}$, respectively. $Q_{10}$ values ranged $3.17\sim5.51$ between 10 and $15^{\circ}C,\;1.87\sim2.10$ between 15 and $20^{\circ}C$ and $1.08\siml.24$ between 20 and $25^{\circ}C$, respectively. Fish held in continuous darkness (OL:24D) used consistently less okygen than fish exposed to continuous light (P<0.05). The mean oxygen consumption offish in a 12L:12D photoperiod was higher than that offish in 24L:0D and 0L:24D photoperiods under all temperature treatments except $10^{\circ}C$. The oxygen consumption of fish exposed to the 12L:12D photoperiod was significantly higher during the light phase than during the dark phase under all temperature treatments except $10^{\circ}C\;(P<0.05)$. This study provides empirical data for estimating oxygen consumption of S. inermis under given condition. This result has application for culture management and bioenergetic model for growth of this species.

Effect of Varying Lighting Regimes on Broiler Performance (Broiler생산에 있어 점등방법이 그 생산능력에 미치는 영향)

  • 유창우;오세정
    • Korean Journal of Poultry Science
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    • v.14 no.1
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    • pp.55-61
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    • 1987
  • Four treatments were conducted to determine the effects of lighting regimes on the body weight gain and feed conversion of broiler chicks during 7 weeks: 1) 24 hours consistant lighting regime, 2) intermittent lighting regime of 1 hour lighting and 3 hours dark, 3) 20 hours lighting and 4 hours dark regime, 4) natural lighting regime. Each treatment was composed of three replications and 180 broiler male chicks were used in this experiment (45 chicks for each treatment,15 chicks for each replication). The results of this experiment were summarized as follows; 1. The body weight gains of 1 hour lighting+3 hours dark regime were heavier than those of any other treatments during whole period, but no significant differences were found. In 1-4 weeks, the body weight gains of 1 hour lighting+3 hours dark regime and 20 hours lighting+4 hours dark regime were much heavier than those of natural lighting regime and 24 hours consistant lighting regime, but in 5-7 weeks, no differences were found among the 4 treatments. 2. The feed conversions of 1 hour lighting+3 hours dark regime were improved more than those of any other treatments during whole period, but no significant differences were found. In 1-4 weeks, the feed conversions of 1 hour lighting+3 hours dark regime and 20 hours lighting+4 hours dark regime were much more improved than those of natural lighting regime and 24 hours consistant lightine regime, but in 5-7 weeks, no differences were found among the 4 treatments. 3. These results indicated that the intermittent lighting regimes such as 1 hour lighting+3 hours dark and 20 hours lighting+4 hours dark are more efficient on the body weight gain and feed conversion of broiler chicks than natural lighting regime and 24 hours consistant lighting regime.

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Steroidal Saponins from Dracaena humilis (Dracaenaceae) and their Chemotaxonomic Significance

  • Mouzie, Cedric Mbiesset;Ponou, Beaudelaire Kemvoufo;Fouedjou, Romuald Tematio;Teponno, Remy Bertrand;Tapondjou, Leon Azefack
    • Natural Product Sciences
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    • v.27 no.2
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    • pp.122-127
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    • 2021
  • A new steroidal saponin, (23S,24S)-spirosta-5,25(27)-diene-1𝛽,3𝛽,23,24-tetrol 1-O-((2,3-diacetyl-α-L-rhamnopyranosyl)-(1→2)-[𝛽-D-xylopyranosyl-(1→3)]-α-L-arabinopyranoside)-24-O-𝛽-D-glucopyranoside (humilisoside) together with the known 𝛽-sitosterol 3-O-glucopyranoside, adenosine, dioscin, and methylprotodioscin were isolated from the leaves of Dracaena humilis. Their structures were elucidated by spectral techniques including mass spectrometry (ESIMS, HRESIMS, tandem MS-MS), 1D NMR (1H, 13C NMR), 2D NMR (HSQC, 1H-1H COSY, HMBC, NOESY), chemical method as well as by comparison with spectroscopic data reported in the literature. The chemotaxonomic significance of the isolation of these compounds is discussed. This is the first report on the phytochemical investigation of D. humilis.