• Title/Summary/Keyword: Damage tolerance

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Confirmation of Drought Tolerance of Ectopically Expressed AtABF3 Gene in Soybean

  • Kim, Hye Jeong;Cho, Hyun Suk;Pak, Jung Hun;Kwon, Tackmin;Lee, Jai-Heon;Kim, Doh-Hoon;Lee, Dong Hee;Kim, Chang-Gi;Chung, Young-Soo
    • Molecules and Cells
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    • v.41 no.5
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    • pp.413-422
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    • 2018
  • Soybean transgenic plants with ectopically expressed AtABF3 were produced by Agrobacterium-mediated transformation and investigated the effects of AtABF3 expression on drought and salt tolerance. Stable Agrobacterium-mediated soybean transformation was carried based on the half-seed method (Paz et al. 2006). The integration of the transgene was confirmed from the genomic DNA of transformed soybean plants using PCR and the copy number of transgene was determined by Southern blotting using leaf samples from $T_2$ seedlings. In addition to genomic integration, the expression of the transgenes was analyzed by RT-PCR and most of the transgenic lines expressed the transgenes introduced. The chosen two transgenic lines (line #2 and #9) for further experiment showed the substantial drought stress tolerance by surviving even at the end of the 20-day of drought treatment. And the positive relationship between the levels of AtABF3 gene expression and drought-tolerance was confirmed by qRT-PCR and drought tolerance test. The stronger drought tolerance of transgenic lines seemed to be resulted from physiological changes. Transgenic lines #2 and #9 showed ion leakage at a significantly lower level (P < 0.01) than ${\underline{n}}on-{\underline{t}}ransgenic$ (NT) control. In addition, the chlorophyll contents of the leaves of transgenic lines were significantly higher (P < 0.01). The results indicated that their enhanced drought tolerance was due to the prevention of cell membrane damage and maintenance of chlorophyll content. Water loss by transpiration also slowly proceeded in transgenic plants. In microscopic observation, higher stomata closure was confirmed in transgenic lines. Especially, line #9 had 56% of completely closed stomata whereas only 16% were completely open. In subsequent salt tolerance test, the apparently enhanced salt tolerance of transgenic lines was measured in ion leakage rate and chlorophyll contents. Finally, the agronomic characteristics of ectopically expressed AtABF3 transgenic plants ($T_2$) compared to NT plants under regular watering (every 4 days) or low rate of watering condition (every 10 days) was investigated. When watered regularly, the plant height of drought-tolerant line (#9) was shorter than NT plants. However, under the drought condition, total seed weight of line #9 was significantly higher than in NT plants (P < 0.01). Moreover, the pods of NT plants showed severe withering, and most of the pods failed to set normal seeds. All the evidences in the study clearly suggested that overexpression of the AtABF3 gene conferred drought and salt tolerance in major crop soybean, especially under the growth condition of low watering.

Cold Tolerance Characteristic Test of High Yield Tongil-type Rice Breeding Lines for Processing

  • Kang-Su Kwak;Sea-Kwan Oh;Kuk-Hyun Jung;Dae-Ha Seo
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.278-278
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    • 2022
  • Recently, the yield potential of high yield Tongil-type rice varieties has greatly increased, reaching 817kg/10a(Geumgang No.1). Moreover, in order to promote rice consumption and strengthen the competitiveness of the rice processing industry, the R&D of high yield Tongil-type rice varieties for each processing purpose, such as rice noodles, grain feed and industrial materials, has been continuously carried out. However, because Tongil-type rice varieties or lines are generally very vulnerable to cold damage, cold tolerance test can be said to be absolutely necessary to improve the cultivation safety. This study is the result of the seedling and field cold tolerance characteristic tests carried out in 2021 of high yield Tongil-type rice breeding lines. For the cold tolerance characteristic test of seedlings, total 303 high yield rice breeding lines for processing were treated in cold water of 13℃ and irrigation depth of 4cm for 10 days from the third-leaf age, then it was evaluated by comparing the degree of discoloration and withering with the checked varieties(Boramchan, Hanahreum No.2). Also, for the test of field, total 186 high yield rice breeding lines for processing were treated in cold water of 17℃ by keeping pouring day and night from 30 days after transplanting to ripening stage, then it was evaluated by comparing the degree of discoloration, delay of heading, shortening rate of stem length and percent of fertile grain etc. with the checked varieties. And the cold tolerance evaluative criteria were classified as strong(1~3), medium(4~6) and weak(7~9) in overall cold tolerance. (Seedling test) As for the degree of cold tolerance of the check variety, 'Boramchan' and 'Hanahreum No.2' showed a response of 'medium' and 'weak', respectively. However, there was no 'strong' line in the high yield rice breeding lines, 2 lines showed a 'medium' response, and 301 lines showed a 'weak' response. Therefore, except for a few lines(0.7%), most lines(99.3%) showed a 'weak' response. (Field test) In terms of the overall cold tolerance of the check variety, both 'Boramchan' and 'Hanahreum No.2' showed a 'medium' response. Similarly, there was no 'strong' line in the case of high yield rice breeding lines, 20 lines showed a 'medium' response, and 166 lines showed a 'weak' response. Therefore, except for some(10.8%) lines, most(89.2%) lines showed a 'weak' response. From the above results, we selected about 100 individuals with less seed shattering and degeneration of the ear tip, and with a relatively high percent of fertile grain, and are continuing to select lines with improved cold tolerance in the F4~F5 group in this year. As such, most of the Tongil-type rice varieties have poor cold tolerance and thus have low cultivation safety at low temperatures. However, it is important to select improved lines through generational progress because there are some lines that still have a certain level of cold tolerance among them.

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Creep Deformation and Rupture Behavior of Alloy 690 Tube (Alloy 690 전열관의 크리프 변형 및 파단 거동)

  • Kim, Woo-Gon;Kim, Jong-Min;Kim, Min-Chul
    • Transactions of the Korean Society of Pressure Vessels and Piping
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    • v.16 no.1
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    • pp.49-55
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    • 2020
  • Creep rupture data for Alloy 690 steam generator tubes in a pressurized water reactor are essentially needed to demonstrate a severe accident scenario on thermally-induced tube failures caused by hot gases in a damaged reactor core. The rupture data were obtained using the tube specimens under different applied-stress levels at 650℃, 700℃, 750℃, 800℃, and 850℃. Important creep constants were proposed using various creep laws in terms of Norton power law, Monkman-Grant (M-G) relation, damage tolerance factor (λ), and Zener-Hollomon parameter (Z). In addition, a creep activation energy (Q) value for Alloy 690 tube was reasonably determined using experimental data. Creep behaviors such as creep strength, creep rates, rupture elongation showed the results of temperature dependence well. Modified M-G plot improved a correlation of the creep rate and rupture life. Damage tolerance factor for Alloy 690 tubes was found to be λ =2.20 in an average value. Creep activation energy for Alloy 690 tube was optimized for Q=350 (kJ/mol). A plot of Z parameter obeyed a good linearity, and the same creep mechanism was inferred to be operative in the present test conditions.

High-Temperature Mechanical Behaviors of Type 316L Stainless Steel (Type 316L 스테인리스강의 고온 기계적 거동)

  • Kim, Woo-Gon;Lee, Hyeong-Yeon
    • Transactions of the Korean Society of Pressure Vessels and Piping
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    • v.16 no.1
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    • pp.92-99
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    • 2020
  • High-temperature mechanical behaviors of Type 316L stainless steel (SS), which is considered as one of the major structural materials of Generation-IV nuclear reactors, were investigated through the tension and creep tests at elevated temperatures. The tension tests were performed under the strain rate of 6.67×10-4 (1/s) from room temperature to 650℃, and the creep tests were conducted under different applied stresses at 550℃, 600℃, 650℃, and 700℃. The tensile behavior was investigated, and the modeling equations for tensile strengths and elongation were proposed as a function of temperature. The creep behavior was analyzed in terms of various creep equations: Norton's power law, modified Monkman-Grant relation, damage tolerance factor(λ), and Z-parameter, and the creep constants were proposed. In addition, the tested tensile and creep strengths were compared with those of RCC-MRx. Results showed that creep exponent value decreased from n=13.55 to n=7.58 with increasing temperature, λ = 6.3, and Z-parameter obeyed well a power-law form of Z=5.79E52(σ/E)9.12. RCC-MRx showed lower creep strength and marginally different in creep strain rate, compared to the tested results. Same creep deformation was operative for dislocation movement regardless of the temperatures.

Evaluation on Tensile Properties and Fracture Toughness of Glass Fiber/Aluminum Hybrid Laminates (유리섬유/알루미늄 혼성 적층판의 인장특성과 파괴인성 평가)

  • Woo Sung-Choong;Choi Nak-Sam
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.29 no.6 s.237
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    • pp.876-888
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    • 2005
  • Tensile properties and fracture toughness of monolithic aluminum, fiber reinforced plastics and glass fiber/aluminum hybrid laminates under tensile loads have been investigated using plain coupon and single-edge-notched specimens. Elastic modulus and ultimate tensile strength of GFMLs showed different characteristic behaviors according to the Al kind, fiber orientation and composition ratio. Fracture, toughness of A-GFML-UD which was determined by the evaluation of $K_{IC}$ and $G_{IC}$ based on critical load was similar to that of GFRP-UD and was much higher than monolithic Al. Therefore, A-GFML-UD presented superior fracture toughness as well as prominent damage tolerance in comparison to its constituent Al. By separating Al sheet from GFMLs after the test, optical microscope observation of fracture zone of GFRP layer in the vicinity of crack tip revealed that crack advance of GFMLs depended on the orientation of fiber layer as well as Al/fiber composition ratio.

A Study on Statistical Characteristics of Fatigue Life of Carbon Fiber Composite (탄소섬유 복합재 피로수명의 통계적 특성 연구)

  • Joo, Young-Sik;Lee, Won-Jun;Seo, Bo-Hwi;Lim, Seung-Gyu
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.47 no.1
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    • pp.35-40
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    • 2019
  • The objective of this paper is to identify the fatigue properties of carbon-fiber composite which is widely applied for the development of aircraft structures and obtain data for full-scale fatigue test. The durability and damage tolerance evaluation of composite structures is achieved by fatigue tests and parameters such as fatigue life factor and load enhancement factor. The specimens are made with carbon-fiber/epoxy UD tape and fabric prepreg. Fatigue tests are performed with several stress ratios and lay-up patterns. The Weibull shape parameters are analyzed by Sendeckyj model and individual fatigue lives with Weibull distribution. And the fatigue life factor and load enhancement factor considering reliability are evaluated.

Impact Property of S-2 Glass Woven Composites with Different Matrices and Stitching (S-2 유리섬유 평직복합재의 기지재료 및 스티칭에 따른 충격 특성 비교)

  • Byeon, Jun-Hyeong;Hwang, Byeong-Seon;Eom, Mun-Gwang;Lee, Jeong-Hun;Nam, Won-Sang;Song, Seung-Uk;Lee, Chang-Hun
    • Proceedings of the Korean Society For Composite Materials Conference
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    • 2005.11a
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    • pp.31-34
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    • 2005
  • For the damage tolerance improvement of conventional laminated composites, stitching process has been utilized for providing through-thickness reinforcements. 2D prefonl1S were stacked with S-2 glass plain weave, and 3D preforms were fabricated using the stitching process. For the matrix system, epoxy and phenol resins were considered. To examine the damage resistance performance the low velocity drop weight impact test has been carried out, and the impact damage was examined by scanning image. CAI (Compressive After Ih1paet) tests were also conducted to evaluate residual compressive strength. Compared with 2D epoxy composites, 2D phenol composites showed drastic reduction in the compressive strength prior to impact because of the higher contents of voids. The damage area of 2D phenol composites were also larger than that of 2D epoxy composites. However, by introducing the stitching, the damage area of 3D phenol composites was reduced by 60%, while the CAI strength improvement was negligible.

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Kinetic Changes of COX-2 Expression during Reperfusion Period after Ischemic Preconditioning Play a Role in Protection Against Ischemic Damage in Rat Brain

  • Kang, Young-Jin;Park, Min-Kyu;Lee, Hyun-Suk;Choi, Hyoung-Chul;Lee, Kwang-Youn;Kim, Hye-Jung;Seo, Han-Geuk;Lee, Jae-Heun;Chang, Ki-Churl
    • The Korean Journal of Physiology and Pharmacology
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    • v.12 no.5
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    • pp.275-280
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    • 2008
  • A brief ischemic insult induces significant protection against subsequent massive ischemic events. The molecular mechanisms known as preconditioning (PC)-induced ischemic tolerance are not completely understood. We investigated whether kinetic changes of cyclooxygenase (COX)-2 during reperfusion time-periods after PC were related to ischemic tolerance. Rats were given PC by occlusion of middle cerebral artery (MCAO) for 10 min and sacrificed after the indicated time-periods of reperfusion (1, 2, 4, 8, 12, 18 or 24 h). In PC-treated rats, focal ischemia was induced by occlusion of MCA for 24 h and brain infarct volume was then studied to determine whether different reperfusion time influenced the damage. We report that the most significant protection against focal ischemia was obtained in rats with 8 h reperfusion after PC. Administration of indomethacin (10 mg/kg, oral) or rofecoxib (5 mg/kg, oral) 48 h prior to PC counteracted the effect of PC. Immunohistochemical analysis showed that COX-2 and HO-l protein were induced in PC-treated rat brain, which was significantly inhibited by rofecoxib. Taken together, we concluded that the kinetic changes of COX-2 expression during the reperfusion period after PC might be partly responsible for ischemic tolerance.