• Journal of the Korean Society of Environmental Restoration Technology
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• v.12 no.3
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• pp.72-82
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• 2009

Research was initiated to investigate early establislment characteristics and germination pattern of perennial ryegrass (PR, Lolium perenne L.). Eight varieties of PR were evaluated in the study. An alternative environmental condition for a PR germination test required by International Seed Testing Association (ISTA) was applied in the experiment, consisting of 8-hr light at $25^{\circ}C$ and 16-hr dark at $15^{\circ}C$ (ISTA conditions). Daily and cumulative germination pattern were measured and analyzed daily for 30 days. Significant differences were observed in germination pattern, days to the first germination, days to 50% germination, days to 75% germination, and final germination rate. Germination rates were variable among the varieties, being 66.00 to 93.75% at the end of study. There were considerable variations in early germination characteristics and germination pattern among PR varieties. The first germination in all entries tested was initiated from 3 to 5 DAS (days after seeding) under ISTA conditions, being 2 days in differences among the varieties. It was 4 to 7 DAS in days to the 50% germination, being 1 to 3 days later from the first germination and three days were variable among them. Days to the 75% germination were 5.93 to 10.50 DAS under ISTA conditions, being 4.57 days in variety differences. Pennant II and Brightstar II were the fastest varieties. The slowest ones were Revenge GLX and Brightstar SLT. Considering days to the first germination, days to 75% germination, and germination pattern etc, Pennant II and Brightstar II were regarded as excellent varieties under ISTA conditions. From tills study, information on differences in germination characteristics and pattern would be usefully applied for golf course design and construction, when established with PR.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.43 no.3
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• pp.157-160
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• 1998

To find out the effects of humidification and hardening of rice (Oryza sativa L. cv. Ilpumbyeo) seeds on the germination, both normal and artificially aged seeds with 60% germination rate were humidified at 40, 60, and 80% RHs and $25^{\circ}C$ for five weeks or hydrated for 6, 12, 18, and 24 hours at $25^{\circ}C$ from one to five cycles for hardening. Relative humidity and duration of humidification did not affect the germination rate of normal seeds, while reduced the time to get 50% germination rate ($T_{50}$) by 1.0～1.6 days compared to that of normal seeds at $25^{\circ}C$. Aged seeds humidified at 40 and 60% RHs did not affect the germination rate and $T_{50}$ regardless of relative humidity and duration of treatment, while at 80% RH, the germination rate decreased and $T_{50}$ increased significantly with the duration of humidification. Hardening of normal seeds reduced $T_{50}$ by 0.7～1.1 days without changes in the germination rate. However, the germination rate and $T_{50}$ of aged seeds soaked in water for 6, 12, and 18 hours were similar regardless of soaking/drying cycles, while hardening of artificially aged seeds with 1～4 cycles in 24 hours soaking increased the germination rate by 11-16% and reduced $T_{50}$ by 1.4～2.0 days.

• Korean Journal of Plant Resources
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• v.13 no.1
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• pp.18-24
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• 2000

Pepper (Capsicum annum L.) used as a spice or fruit vegetable has been transplanted for cultivation to accompany the production of its seedlings in general. The experiment was done to measure the effect of its cultivars (Daemyng; Wanggochu), priming (chemicals; concentration; period), light quality (red; far-red; blue; dark) during priming, and germination temperature (25 or 15$^{\circ}C$ constant; 25/15$^{\circ}C$ alternating) on the rate of germination done under incandescent lamps until 9 days after sowing. Seed germination was better when primed with Ca(NO$_3$)$_2$ than with KNO$_3$. Priming for 6 to 12 days using the former chemical enhanced the final germination rate and shortened the elapsed days to 50% germination, $T_{50}$, compared to no-priming. Under $25^{\circ}C$ constant germination temperature, primed seeds at darkness or far-red light showed the highest rate until 5 days after sowing or the lowest one since 7 days after sowing, respectively. The germination response at 25/15$^{\circ}C$ alternating temperature did not followed the lowest rate of 15$^{\circ}C$ constant germination temperature but inclined to that of $25^{\circ}C$ constant recorded the greatest one. Under 3 germination temperature red light treated during priming elevated the rate since 7 days after sowing compared to the other light treatments, and the germination rate of each cultivar was affected by no-priming or priming, light quality during priming. which imply their interaction to the seed germination.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.13 no.4
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• pp.30-41
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• 2010

Research was initiated to investigate germination characteristics and germination pattern of creeping bentgrass (CB, Agrostis palustris Huds.). Seven varieties of CB were evaluated in the study. An alternative condition for a CB germination test required by International Seed Testing Association (ISTA) was applied in the experiment, consisting of 8-hr light at $25^{\circ}C$ and 16-hr dark at $15^{\circ}C$ (ISTA conditions). Daily and cumulative germination patterns were measured and analyzed on a daily basis. Significant differences were observed in germination pattern, days to the first germination, days to 50% germination, days to 85% germination, and germination percentage. Germination rates were different among the treatments at the end of study. There were significantly considerable variations in early germination characteristics and germination pattern among CB varieties. The first germination in all entries was initiated between 4 and 5 DAS (days after seeding) under ISTA conditions. Generally, the first germination percentage was 5 to 25%. However, such Penn-series varieties as Penn A-1, Penn A-4 and Penncross were much more vigorous over others, resulting in 50 to 75% in the first germination percentage. It took 4 to 10 DAS in reaching to days to the 50% germination, being 5 days in difference among the varieties. Days to the 85% germination were 5.90 to 11.75 DAS under ISTA conditions, being 5.85 days in variety difference. It means 1 to 2 days after days to 50% germination. Penn A-1 was the fastest variety, while T-1 the slowest one. Considering days to the first germination, days to 85% germination, and germination pattern etc, Penn A-1, Penn A-4, Penncross and L-93 were regarded as excellent varieties under ISTA conditions. From this study, in-depth information on germination characteristics and pattern would be usefully applied for golf course design and construction, when established with CB.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.17 no.5
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• pp.1-17
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• 2014

Research was initiated to investigate early establishment characteristics and germination pattern of Kentucky bluegrass (KB, Poa pratensis L.) under natural room temperature and to analyze differences between alternative and natural conditions for a practical application. Fifteen cultivars of KB were evaluated in the study. Daily and cumulative germination pattern were measured and analyzed on a daily basis. Differences were observed in germination percentage, germination pattern, days to the first germination and days to 75% germination. Germination percentage was variable with cultivars at the end of study. It was 75.75 to 90.25% under natural condition. There were considerable variations in early germination characteristics and germination pattern among KB cultivars according to germination conditions. The first germination in all entries tested was initiated between 6 and 9 DAS (days after seeding) under ISTA (International Seed Testing Association) condition. But it was observed between 9 and 12 DAS under natural condition, being 3 to 5 days later as compared with ISTA condition. On the first date of a germinating process, the germination rate was 0.25% to 25.00% under ISTA condition, while 0.50% to 13.25% under natural condition. Days to the 75% germination were 15.08 to 28.80 DAS under ISTA condition and 17.78 to 28.75 DAS under natural condition. Midnight II, Excursion and Midnight were the fastest cultivars under ISTA condition, while Odyssey the fastest one under natural condition. Regardless of germination condition, the slowest cultivar was Voyager II, being over 4 weeks to 75% germination percentage. For the most cultivars, days to 75% germination under natural room temperature condition were 1 to 8 days slower than under ISTA condition. From this study information on differences in germination characteristics and pattern among cultivars would be usefully applied for KB establishment such as golf course construction. Prior to on-site seeding, a comprehensive germination test is practically recommended due to a possible decline in germination capability during domestic distribution and storage after import.

• Weed & Turfgrass Science
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• v.3 no.1
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• pp.29-40
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• 2014

Research was initiated to investigate early establishment characteristics and germination pattern of Kentucky bluegrass (KB, Poa pratensis L.). Fifteen cultivars were evaluated under alternative conditions (8 hours light at $25^{\circ}C$ and 16 hours dark at $15^{\circ}C$). Significant differences were observed in germination characteristics and germination pattern among KB cultivars. A final germination percentage differed in cultivars, being 75.25 to 89.50%. The first germination was initiated between 6 and 9 DAS (days after seeding). As for the first germination percentage, 'Brilliant' and 'Midnight II' produced 14.50% and 23.00%, respectively, while the others were most below 5%. Days to 75% germination were between 15.08 and 28.80 DAS. 'Excursion', 'Midnight II', 'Odyssey', 'Midnight' and 'Courtyard' were fastest. The slowest cultivar was 'Voyager II', being over 28 DAS, which were 13 to 14 days slower than the fastest ones. Considering the first germination percentage, days to the first germination, days to 75% germination, and germination pattern, 'Midnight II', 'Excursion' and 'Midnight' were regarded as excellent cultivars under alternative conditions. From this study, information on differences in germination characteristics and patterns would be practically useful for a golf course construction when established with KB.

• Asian Journal of Turfgrass Science
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• v.25 no.1
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• pp.1-10
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• 2011

This study was conducted to investigate early germination characteristics and germination pattern of new cultivars of creeping bentgrass (CB, Agrostis pall/stris Huds.) under natural room temperature conditions and to analyze differences between alternative and natural conditions. Seven cultivars of CB were tested in the study. Daily and cumulative germination patterns were measured and analyzed on a daily basis. Significant differences were observed in germination pattern, days to the first germination, days to 50% germination, days to 85% germination, and germination rate among cultivars under different environments. The first germination in all entries was initiated between 4 and 5 days after seeding (DAS) under alternative conditions, while between 6 and 8 DAS under natural conditions, being 2 to 3 days later as compared with that of alternative conditions. As for the first germination percentage, it was generally 5 to 25% under alternative conditions. However, 'Penn A-1', 'Penncross', and 'Penn A-4' cultivars showed a vigorous germination, producing 50 to 70%. Under natural room temperature conditions, it was 5 to 10% for the most entries, except 'Penncross', 'L-93', and 'Penn A-4' cultivars which vigorously produced 20 to 50%. Days to the 50% germination in CB resulted in 4 to 10 DAS under alternative conditions, while 6 to 15 DAS under natural conditions, being 2 to 5 days later than under alternative conditions. Days to the 85% germination were 5.90 to 11.75 DAS under alternative conditions and 7.95 to 12.50 DAS under natural conditions. 'Penn A-1' and 'L-93' were the fastest cultivars under alternative and natural conditions, respectively. Regardless of the temperature conditions, the longest cultivar in germination was 'T-1', being over 11 DAS. In-depth comparative data on germination characteristics and germination patterns among CB cultivars would be usefully applied for a practical CB turf establishment in golf course construction etc.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.44 no.3
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• pp.263-266
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• 1999

Tobacco (Nicotiana tabacum L. ‘KF109’) seeds were primed in polyethylene glycol 6000 (PEG 6000) solutions to determine a) what osmotic potential of the solution would be optimal for priming, i.e., critical potential level for preventing germination, and b) what temperature and duration would be the most effective in priming. The germination was completely prevented below -0.8 MPa of PEG 6000, that indicates a optimum water potential for seed priming. Seeds were primed for 0, 1, 2, 3, 5, 10 and 15 days at 15, 20 and $25^{\circ}C$, respectively, under the-0.8 MPa PEG 6000 solution to find out the most effective temperature and duration for priming. The effectiveness of priming, particularly in germination speed, was observed more distinctly when the primed seeds were germinated at 15$^{\circ}C$ than 2 5$^{\circ}C$. The greatest reduction of the time to 50% germination (T/sob 50/) was when the seeds were primed at $25^{\circ}C$. The reduction rate of the $T_{50}$ was rapid when primed from 1 day to 8 days and then slowed down in the seeds primed for longer than 8 days. The time from 10 to 90% germination ( $T_{10-90}$ increased in the primed seeds for longer than 8 days which showed the reversed effect of synchronous germination. However, $T_{50}$ was reduced continuously in the seeds even primed over 8 days. Thus, the optimum condition for tobacco seeds priming with PEG 6000 solution was -0.8 MPa in osmotic potential of the solution at $25^{\circ}C$ for 8 days.ays.

• Journal of the Korean Society of Tobacco Science
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• v.25 no.1
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• pp.47-52
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• 2003

The purposes of this study were to examine effect by Solid Matrix Priming(SMP) ; method far enhanced rapid and uniform seedling emergence in tobacco seeds. The results were as follows. No enhancement of germination rate was by SMP treatment. In KF114 during 9 days treatment and in KB108 during 7 days treatment, germinative energy was highest, and T50 and mean germination time were the shortest. Cells in embryo and hypocotyl were divided and extended during SMP treatment. The contents of fatty acid were increased in SMP treatment. The changes of glucose content were little.

• Weed & Turfgrass Science
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• v.6 no.3
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• pp.235-248
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• 2017

This study was initiated to investigate seed germinating vigor, germination speed, and establishment characteristics of Festuca species under natural conditions ($5-25^{\circ}C$). Germination rate was 62.0% in CRF 'Audubon' to 97.0% in TF 'Olympic Gold'. It was greater with TF over fine-type fescues, being TF>SF>CF, HF>CRF. The germination speed, measured as days to seed germination of 50% through 80%, was fastest with TF, medium to high with CF, medium with HF and SF, and slowest with CRF. However, it was approximately 4 days slower under natural conditions, as compared with that under alternative conditions ($15-25^{\circ}C$). Germination peak time was much faster with TF. It ranged from 0.73 to 1.84 days with TF, being 1.45 days in average. But it varied with 2.16 to 11.75 days among fine-type species. The peak time was longer in order of SFCF>SF>HF>CRF.